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Citations for Roche :
451 - 500 of 577 citations for u 78057 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Epidermal and dermal sheets were prepared after incubation overnight at 4°C with 4 U/ml dispase II (Roche) in PBS by gently separating dermis and epidermis each as intact sheets (Rahn et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... in a total volume of 40 µl to 2.4 pmole trypSL in the presence of 1 mM ATP and 20 U RNasin (Roche) in vaccinia capping buffer ...
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bioRxiv - Immunology 2022Quote: ... tissues were minced and placed into gentleMACS C tubes (Miltenyi 130-093-237) containing 15 mL enzyme digestion solution (10 mM HEPES, 0.2 U/mL Liberase TM [Roche 5401127001] ...
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bioRxiv - Genomics 2020Quote: ... the pellets were washed with ice-cold nuclei suspension buffer (1X PBS containing 2% BSA and 0.2 U/µl Protector RNase Inhibitor, ROCHE) and filtered through a 30μm cell strainer (Fisher Scientific) ...
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bioRxiv - Genomics 2021Quote: ... Then 100 nL of KAPA PCR mix (2.775 X HiFi Buffer, 0.85 mM dNTPs, 0.05 U KAPA HiFi polymerase / µL)(Roche Cat# 07958846001) was added to all wells containing a single nucleus via two 50 nL filtered dispenses ...
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bioRxiv - Bioengineering 2020Quote: Muscles were dissected and minced to a pulp using forceps then digested with collagenase B solution (0.75 U/mL final, from Roche) with dispase II (1.2 U/mL final ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were harvested and incubated in buffer A (50 mM MMT pH 8.0/300 mM KCl/10 mM MgCl2/5 % glycerol) containing 1 g L-1 lysozyme/2.5 U mL-1/SmDNAse//complete protease inhibitor cocktail (Roche) for 1 h prior to lysis using EmulsiFlex C5 (Avestin ...
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bioRxiv - Neuroscience 2021Quote: ... the beads were resuspended in EMSA 1X with 100 U/ml RNAse inhibitor and 1X protease inhibitor cocktail (Roche). FRMP binding assay was performed by incubating biotinylated transcripts (250 ng ...
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bioRxiv - Cell Biology 2021Quote: ... 12-20 ml of pre-warmed to 37 °C enzyme buffer solution (EBS) with 2.3 U of Liberase Blendzyme 3 recombinant collagenase (Roche) were cannulated into the vena cava to isolate hepatocytes ...
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bioRxiv - Biochemistry 2022Quote: ... 10 mM MgCl2, 10% glycerol, 300 mM NaCl, 0.2 U/mL DNase 1, 1 mM PMSF, complete proteinase inhibitor mixture; Roche) and homogenized with an EmulsiFlex for 20 min ...
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bioRxiv - Microbiology 2022Quote: ... cells were resuspended in a 50 mM sodium phosphate buffer at pH 7.8 (buffer A) supplemented with DNase I (100 U, DNase I recombinant, RNase-free Roche), and disrupted using a French Pressure Cell (3 cycles at 1100 psi) ...
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bioRxiv - Immunology 2022Quote: Enrichment and staining of lung immune cells were performed as detailed elsewhere [2,3] after treatment with 400 U/ml type IV collagenase and DNase I (Roche) for a 30-minute incubation at 37°C and homogenization by use of GentleMacs (Miltenyi Biotech) ...
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bioRxiv - Biophysics 2022Quote: ... The sinoatrial node explants from old animals were first incubated for 20 min in Low-Calcium Tyrode’s containing twice the concentration of collagenase B used in the young (Cf = 1.08 U/ml, Roche 11088807001), followed by the same 30 min digestion with the three other enzymes at the same concentration used for young tissues ...
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bioRxiv - Neuroscience 2020Quote: ... DRGs were dissociated in 1 μg/ml collagenase A plus 2.4 U/ml dispase II (enzymes, Roche Applied Sciences) in HEPES-buffered saline (HBSS ...
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bioRxiv - Neuroscience 2020Quote: Whole cerebral organoids were washed three times in 1× PBS and dissociated in 2 mL of Accutase (StemPro) containing 0.2 U/μL Dnase I (Roche) for 45 min at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... Platelets (3 x 105 platelets/μl) were allowed to spread on the coated surface after the addition of 0.01 U/ml thrombin (Roche) and Ca2+ ...
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bioRxiv - Microbiology 2021Quote: ... RNA samples were incubated for 30 min at 37°C with 20 U of RNase-free DNase I (Roche) in a final reaction volume of 30 μl ...
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bioRxiv - Biochemistry 2020Quote: ... 100 mM KCl, 1 mM MgCl2, 0.5 mM β-mercaptoethanol, 0.1 mM GTP, 3 U/ml benzonase, 1X Roche Complete EDTA-free protease inhibitor ...
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bioRxiv - Biophysics 2021Quote: ... Platelet activation was achieved by complementing the Tyrodes buffer with 2 mM Ca2+ and the addition of 0.01 U/ml Thrombin (10602400001, Roche) to the platelet suspension.
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bioRxiv - Immunology 2020Quote: ... minced with scissors and digested at 37°C for 20-30 minutes in digestion buffer (0.52 U/ml Liberase TM [Roche] and 60 U/ml DNase I [Roche] in RPMI 1640 [Cellgro] with 5% fetal bovine serum) ...
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bioRxiv - Neuroscience 2021Quote: The tissue was dissociated in 300 µL of 0.25% trypsin-EDTA solution supplemented with 10 U/mL recombinant DNase I (Roche) for 15 minutes at 37°C ...
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bioRxiv - Genomics 2021Quote: ... KAPA PCR mix (2.775 X HiFi Buffer, 0.85 mM dNTPs, 0.05 U KAPA HiFi polymerase / µL, Roche Cat# 07958846001). The chip was sealed for heated incubation and spun down at 1200 x g for 1 min after each dispense ...
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bioRxiv - Immunology 2020Quote: Single-cell suspensions of the lung were prepared by digesting minced lung lobes for 30 min at 37°C with 0.2 U/ml Liberase TL (Roche) and 80 U/ml DNase (Life Tech ...
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bioRxiv - Genomics 2021Quote: ... containing 25 Abs260 ribosome units (1 A260 = 12 μg/μL) [5] were digested with 60 U of MNase (Roche) and 60 U of SUPERase·In (Ambion ...
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bioRxiv - Microbiology 2022Quote: ... according to the manufacturer’s instructions and maintained in RPMI GlutaMax supplemented with 10% FCS and 30 U/ml recombinant IL-2 (Roche).
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bioRxiv - Neuroscience 2023Quote: ... Supernatants were removed and pellets were re-suspended in 2% BSA/PBS containing RNase inhibitor (0.2 U/μL) (Roche). Nuclei were incubated with fluorescently conjugated antibodies against the neuronal marker NEUN (RBFOX3/NEUN (1B7 ...
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bioRxiv - Molecular Biology 2023Quote: ... Mouse atria were minced and digested with mild agitation for 30 minutes at 37°C in DMEM containing 1% P/S and 0.14 Wunsch U ml-1 Liberase (5401119001, Roche). MAFs were enriched via negative selection with magnetic beads against mouse CD45 (leukocytes) ...
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bioRxiv - Cancer Biology 2022Quote: ... Triton X-100 in PBS for 20 min and followed by 1 hr DNAse (1,000 U/ml; #04536282001, Roche) treatment at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: ... ovaries were crosslinked in 250 µL YSS buffer (50 mM pH 8.0 Tris HCl, 50 mM NaCl, 0.1% NP40, 1 U/mL of RNAsin (Roche, 3335402001), protease inhibitor (Thermo Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... were placed in 250µL of ice-cold disassociation media (sterilized 1x PBS and 0.04% bovine serum albumin, 1.2 U/mL Dispase II (Roche 04942078001), 1 mg/mL Collagenase IV (Gibco 17104–019) ...
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bioRxiv - Cancer Biology 2023Quote: ... Bones were cut into small fragments using scissors in a dissociating media containing 10 U/ml DNase I and Collagenase P (1.2 mg/ml, 11213865001, Roche). Bones and BM were digested at 37°C for 20 min under agitation ...
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bioRxiv - Immunology 2022Quote: ... and the lamina propria was minced and digested in RPMI medium containing 2.5 mg/ml Liberase TL and 20 U/ml DNase I (both from Roche) at 37°C for 1 h ...
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bioRxiv - Genetics 2022Quote: ... Samples were divided into three 500 µL aliquots that were then digested with 22.5 U of MNase (Nuclease S7 from Roche) at 50 min at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 μL of 180 mM of adenosine triphosphate (ATP) and 0.5 U of Glucose-6-phosphate dehydrogenase(G6PDH) (Roche). After the absorbance at 340 nm was recorded for 20 min to determine a baseline ...
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bioRxiv - Microbiology 2024Quote: ... Lungs of infected mice were excised and digested enzymatically at 37°C for 60 min in 10 mL of digest buffer (0.34 U/mL LiberaseTM (Roche) and 100 ug/mL DNaseI (ThermoFisher) ...
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bioRxiv - Microbiology 2024Quote: ... previously digested with the same enzymes and incubated for 1 hour at 37°C with 1 µl of alkaline phosphatase from calf intestine (20.5 U/µl; Roche). Ligation reactions were carried out overnight at 16°C using 1µl of T4 DNA Ligase (20 U/µl ...
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bioRxiv - Immunology 2024Quote: ... resuspended in RPMI-1640 (Fisher) supplemented with 10% Fetal Bovine Plasma (FBS) and 20 U/mL deoxyribonuclease I (Roche), concentrated by centrifugation (300 x g for 15 min at 20°C) ...
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bioRxiv - Molecular Biology 2024Quote: ... The pellet was resuspended with 1 ml of single nuclei wash buffer containing 0.2 U/µl RNase inhibitor (3335402001, Roche) and transferred to a microcentrifuge tube ...
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bioRxiv - Cancer Biology 2024Quote: The epidermis was separated from the dermal layer via digestion in Dispase II (final conc. 2.7 U/mL in PBS, Roche) for 30 min on ice ...
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bioRxiv - Neuroscience 2024Quote: ... in nuclei resuspension buffer of 1x PBS with 2% BSA and 0.2 U/µl Protector RNase Inhibitor (Roche, USA). Resuspended nuclei were then overlaid on 750 μl of 29% Optiprep in nuclei resuspension buffer before centrifuging at 13,000 g for 30 min at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Any contaminating DNA in the samples was removed by treating 15 µg of RNA with 10 U of DNase I (Roche) for 15 min at 37°C in the presence of 80 U of recombinant RNase inhibitor (Takara Bio) ...
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bioRxiv - Plant Biology 2022Quote: ... The ELVd probe was obtained via in vitro transcription of a linearized plasmid for 1 h at 37°C with 20 U of T3 bacteriophage RNA polymerase (Roche) in 40 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2020Quote: ... 25 mM Tris pH 8.0, 0.05% SDS, 2 mM MgCl2, 10 U/ml benzonase, and cOmplete™ EDTA-free protease inhibitor cocktail [Roche, 27368400]). Extracts were then incubated on ice for 10 min before protein concentration was calculated by Bradford assay (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... thermophila intron were obtained by in vitro transcription of the corresponding linearized plasmids with 20 U of T3 bacteriophage RNA polymerase (Roche) in 20-μl reactions containing 40 mM Tris-HCl ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Paw tissues were minced and digested in 1 mg/ml collagenase A / 2.4 U/ml dispase II (Roche Applied Sciences) in HEPES buffered saline (Sigma ...
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bioRxiv - Bioengineering 2020Quote: ... Tissues were finely diced manually with standard razor blades and digested for 45 min at 37°C with 1.67 Wünsch U/mL (0.5 mg/mL) Liberase TL (Roche Diagnostics, Sigma Aldrich) and 0.2 mg/mL DNase I (Roche Diagnostics ...
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bioRxiv - Neuroscience 2021Quote: ... Coli tRNA at RT for 10 minutes and resuspended in EMSA 1X with 100 U/ml RNAse inhibitor and 1X protease inhibitor cocktail (Roche). The assay was then performed in presence of 250 ng of biotinylated transcript ...
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bioRxiv - Biochemistry 2021Quote: ... 0.1 mg/mL lysozyme (∼20,000 U/mg; Carl Roth) and one tablet cOmplete™ ULTRA EDTA-free protease inhibitor tablet (Roche). Cells were lysed by homogenization using an EmulsiFlexC5 (Avestin Inc. ...
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bioRxiv - Microbiology 2021Quote: ... A second DNase I treatment to remove all residual genomic DNA was performed using 30 U of recombinant DNase I (Roche) and 10 U of Protector RNase inhibitor (Roche ...
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bioRxiv - Developmental Biology 2022Quote: ... and prepared for flow cytometry immunostaining as follows: Matrigel-embedded organoids were digested using a HBSS solution containing 10 U/ml dispase II (Roche) and 125 U/ml of collagenase type IV (Gibco ...