Labshake search
Citations for Roche :
451 - 500 of 6051 citations for Mouse Ras Related Protein M Ras MRAS ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2021Quote: ... and then resuspended in 200 μL of SCE buffer (1 M sorbitol, 10 mM EDTA, 10 mM DTT, 100mM sodium citrate, 1 Roche mini-EDTA-free protease inhibitor tablet per 50 mL ...
-
bioRxiv - Molecular Biology 2019Quote: ... Solidified plugs were transferred in 14 ml tubes with round bottom and incubate with 400 µl/plug of PK buffer (EDTA 0.125 M [pH 9.5], Sarkosyl 1%, PK 1 mg/ml (Roche, ref# 03115801001) and incubated 1-2 hours at 37°C with gentle agitation ...
-
bioRxiv - Genomics 2020Quote: ... before incubation at 37°C with 1 µg of double stranded DNA (atr amplicon, Jones et al., 2003) in the presence of 1x M buffer (Roche). Filtrated supernatants (10 µl ...
-
bioRxiv - Biochemistry 2020Quote: ... The thorax and the abdomen were then washed with 3 ml of pre-chilled 0.1 M KBP pH 7.4 homogenization buffer (HB) containing 1x protease inhibitor (Roche Complete ULTR). The complex was homogenized in 20 ml of HB using 40 ml glass Dounce homogenizer with a loose B pestle (Wheaton Science ...
-
bioRxiv - Neuroscience 2021Quote: ... were dissociated onto poly-d-lysine (50 ug/mL diluted in 0.1 M Borate Buffer, pH 8.6) and laminin (10 ug/mL; Roche, Basel, Switzerland) coated 6 well plastic plate ...
-
bioRxiv - Cell Biology 2021Quote: ... air-dried and resuspended in 50 µl of 8 M urea in 50 mM triethylammoniumbicarbonate including protease inhibitor cocktail (Roche). Afterwards proteins were reduced with 5 mM dithiothreitol for 1h at 25°C and alkylated with 40 mM chloroacetamide for 30 min in the dark ...
-
bioRxiv - Biochemistry 2021Quote: Cells were washed 2x with PBS to remove excess biotin and lysed in highly stringent washing buffer 5 (WB5; 8 M urea, 1% SDS in 1X PBS) supplemented with 1x protease inhibitor cocktail (Roche) and 50 μM NEM ...
-
bioRxiv - Cell Biology 2021Quote: ... 150 mM KOAc, 2 mM MgCl2, 1 mM CaCl2, 0.2 M sorbitol, 10mM PMSF, 2x protease inhibitor cocktails [Roche, 11873580001]), dropped into liquid nitrogen ...
-
bioRxiv - Pathology 2022Quote: ... and brain tissues were homogenized using a Teflon-glass homogenizer in ice-cold homogenization buffer (0.32 M sucrose, 5 mM EDTA, 10 mM Tris-HCl, and pH 7.4, phosphatase inhibitor cocktail tablet; Roche, Mannheim, Germany), centrifuged at 4500 rpm for 10 min at 4°C and the supernatants were collected.
-
bioRxiv - Animal Behavior and Cognition 2022Quote: ... hippocampi from each animal were homogenized in ice-cold Homogenization buffer (2 M sucrose, 500 mM HEPES (pH 7,4)) containing complete Protease Inhibitor (Roche/Sigma) and spun down for 10 min at 1000 x g at 4 °C ...
-
bioRxiv - Biochemistry 2022Quote: ... 250,000 cells (per antibody/cell line combination) were washed twice (20 mM HEPES pH 7.5, 0.15 M NaCl, 0.5 mM spermidine, 1x Roche complete protease inhibitors) and attached to ConA-coated magnetic beads (Bangs Laboratories ...
-
bioRxiv - Developmental Biology 2019Quote: ... The frozen cells and organoids were crushed by using Multi-beads shocker (Yasui Kikai, Japan) and subsequently lysed by sonication in 9.8 M urea with protease inhibitor cocktail (cOmplete™; Roche) and phosphatase inhibitor cocktail (PhosSTOP™ ...
-
bioRxiv - Neuroscience 2019Quote: ... Neurons were washed three times with PBS and harvested directly in 8 M Urea lysis buffer supplemented with phosphatase inhibitor (PhosSTOP, Roche) and protease inhibitor (cOmplete mini EDTA-free ...
-
bioRxiv - Cancer Biology 2020Quote: ... Nuclei were pelleted at 1350xg for 5 min at 4°C and lysed in 5 mL LB2 (10 mM Tris-Cl pH 8.0, 5 M, 200 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, 1 mM PMSF, Roche protease inhibitors) for 10 min at RT with rotation ...
-
bioRxiv - Immunology 2020Quote: Cell lysates from flow-sorted bone marrow osteoclast progenitors grown in presence of M-CSF and RANKL over time were harvested in 1% NP40 lysis buffer containing 1X cOmplete Protease Inhibitor cocktail (Roche). Samples were separated by SDS-PAGE and transferred to nitrocellulose membrane ...
-
bioRxiv - Neuroscience 2020Quote: Mouse brains were homogenized with lysis buffer (50 mM Tris pH 7.6, 0.15 M NaCl and cOmplete protease inhibitor cocktail (Roche Diagnostics 11697498001)) using a Multi-bead shocker MB (Yasui-Kikai) ...
-
bioRxiv - Cancer Biology 2022Quote: ... staining experiments were performed on 4µm thick formalin fixed and paraffin embedded (FFPE) tissue sections using a Ventana automated tissue staining system (BenchMark Ultra, Roche). For IHC ...
-
bioRxiv - Immunology 2022Quote: ... Cell pellets were lysed by the lysis buffer 10 M urea containing the cOmplete™ protease inhibitor cocktail (Roche, #11697498001). The cell tubes were ultrasonicated at 4 ℃ for two cycle (1 min per cycle ...
-
bioRxiv - Neuroscience 2023Quote: ... cells were washed with ice-cold PBS and scraped into lysis buffer (1 mM EPPS, 8 M urea, protease (complete mini, EDTA-free) inhibitors (Roche) and PhosSTOP phosphatase inhibitor (Roche)) ...
-
bioRxiv - Neuroscience 2023Quote: ... Five P2 mouse brains were homogenized in 5 mL of homogenization buffer (5 mM Tris-HCl, 0.32 M sucrose, 1 mM MgCl2) supplemented with EDTA-free protease inhibitors (cOmplete™, Roche), then centrifuged at 1,660 x g for 15 min at 4 °C ...
-
bioRxiv - Molecular Biology 2023Quote: ... 25 μL 1 M spermidine, 500 µL 10% Triton X-100, and 10 ml glycerol in 38 ml dH2O, 1 Roche Complete Protease Inhibitor EDTA-Free (Millipore Sigma 11873580001)) ...
-
bioRxiv - Microbiology 2023Quote: ... Then the different pellets were resuspended for 30 min in denaturing buffer (20 mM Tris, 500 mM NaCl, 6 M Guanidine, 20 mM Imidazole and protease inhibitor cocktail (Roche), pH 7.5) ...
-
bioRxiv - Cell Biology 2023Quote: Cells were washed 2x with 1x PBS to remove excess biotin and lysed in highly stringent washing buffer 5 (WB5; 8 M urea, 1% SDS in 1x PBS) supplemented with 1x protease inhibitor cocktail (Roche) and 50 μM NEM ...
-
bioRxiv - Neuroscience 2023Quote: Mouse brains at 5W were isolated and homogenized in homogenization buffer [(0.32 M sucrose, 10 mM HEPES, 2 mM EDTA and 1× complete protease inhibitor cocktail (Roche Diagnostics), pH 7.4)] ...
-
bioRxiv - Cell Biology 2022Quote: ... and cells were scraped in an ice-cold homogenization buffer (10 mM Tris-HCl pH 7.4, 1 mM EGTA, 0.5 mM EDTA, 0.25 M sucrose, supplemented with Roche protease inhibitor cocktail) and homogenized with ten strokes of a loose-fitting Dounce homogenizer ...
-
Borrelia PeptideAtlas: A proteome resource of common Borrelia burgdorferi isolates for Lyme researchbioRxiv - Biochemistry 2023Quote: ... The bacterial pellets were dispersed in lysis buffer of 8 M urea in 100 mM ammonium bicarbonate and protease inhibitor cocktail (CoMplete, (Roche)) ...
-
bioRxiv - Developmental Biology 2023Quote: ... followed by quick wash with PBS and lysis with 400 µM of lysis buffer (50LJmM Tris pH7.5, 150LJmM NaCl, 1% Triton-X supplemented with 2x protease inhibitor cocktail (Roche, 11836145001), 1x phosphatase inhibitor cocktail (Roche ...
-
bioRxiv - Cell Biology 2023Quote: ... 2 M urea, 0.5% SDS, 1 mM EDTA pH8.0, 1 mM DTT, and 1× protease inhibitor cocktail [Roche, cat#4693132001]). Biotinylated RBPs were captured by affinity purification ...
-
bioRxiv - Bioengineering 2023Quote: ... or 1 M NaCl (for ori designs), 20 µg/mL DNase (#A3778, ITW Reagents) and protease inhibitor cocktail (04693132001, Roche)) ...
-
bioRxiv - Immunology 2023Quote: Cells were washed with PBS and lysed with ice-cold CelLytic M Cell Lysis Reagent containing protease inhibitor cocktail (Roche) and phosphatase inhibitors (Sigma) ...
-
bioRxiv - Immunology 2024Quote: ... Cells were then rinsed fast with ice cold PBS and lysed using M-PER lysis buffer (supplemented with protease inhibitor (Roche) and HALT phosphatase inhibitor (Thermo ScientificTM #87786 ...
-
bioRxiv - Pathology 2024Quote: ... The proteins were redissolved in 8 M Urea/50 mM TEAB buffer containing protease- and phosphatase inhibitors (EDTA free cOmpleteTM, PhosSTOPTM Roche) and treated 30 minutes at 37 °C with 50 units/100 µg tissue benzonase (Sigma Aldrich ...
-
bioRxiv - Cell Biology 2022Quote: ... monoclonal mouse anti-HA (MMS-101R; Covance) or monoclonal mouse anti-GFP antibodies (11–814–460-001; Roche) prior to secondary antibody treatment with polyclonal goat anti-mouse conjugated to horseradish peroxidase (115–035-146 ...
-
bioRxiv - Cell Biology 2022Quote: ... cell lysate was incubated with agarose beads conjugated with either mouse IgG or mouse anti-GFP antibodies (Roche) for 3h at 4°c under gentle agitation ...
-
bioRxiv - Cell Biology 2020Quote: ... mouse anti-GFP (Roche, # 11814460001, 1/1000), rabbit anti-calreticulin (Abcam ...
-
bioRxiv - Cell Biology 2020Quote: ... mouse anti-GFP (1:1000, Roche 11814460001), goat anti-SYP-1 (1:500 ...
-
bioRxiv - Molecular Biology 2020Quote: ... mouse anti-GFP (7.1+13.1, Roche, 11814460001) and normal rabbit IgG (Millipore 12-370).
-
bioRxiv - Cell Biology 2019Quote: ... HRP-conjugated anti-mouse secondary antibody (Roche) and ECL (PIERCE ...
-
bioRxiv - Cell Biology 2019Quote: ... anti-myc from mouse (9E10; 11667149001; Roche) and anti-HA from mouse (12CA5 ...
-
bioRxiv - Microbiology 2019Quote: ... anti-GFP mouse 1:100 (11814460001; Roche), anti-HA rabbit 1:50 (H6908 ...
-
bioRxiv - Microbiology 2019Quote: ... mouse anti-GFP (1:1000, Roche Diagnostics) and rabbit anti-mCherry (1:1000 ...
-
bioRxiv - Cell Biology 2019Quote: ... mouse anti-GFP (11814460001; Roche, 1:200). Slides are coated with 100 µl of primary antibody for 1 hour followed by washing with PBS three times with 5 min incubation each time ...
-
bioRxiv - Genetics 2021Quote: Mouse bi-clonal anti-GFP antibody (Roche Diagnostics ...
-
bioRxiv - Molecular Biology 2021Quote: ... primary mouse anti-DIG (Sigma Roche 11333062910) was added at the dilution of 1 ...
-
bioRxiv - Microbiology 2021Quote: ... 1:1000 anti-GFP (Mouse. Roche 11814460001), 1:5000 anti-rabbit HRP (Cell Signaling Technology ...
-
bioRxiv - Cell Biology 2021Quote: ... 10µg anti-GFP mouse (Roche, Catalog #11814460001) and 1mL of NP-40 lysis buffer was added to the DYNAbeads tube ...
-
bioRxiv - Cell Biology 2021Quote: ... anti-GFP (mouse monoclonal, Roche, 1:400), anti-SYP-1 (goat ...
-
bioRxiv - Cell Biology 2021Quote: ... Mouse anti-GFP monoclonal antibody (Roche, 11814460001) was covalently coupled to magnetic beads at 12 μg antibody per 100 μL Dynabeads Protein A (ThermoFisher) ...
-
bioRxiv - Microbiology 2021Quote: ... mouse anti-HA antibody (Roche; 1:1000), rabbit-anti-γH2A.X antibody (Cell signaling cat # 9718S ...
-
bioRxiv - Cell Biology 2021Quote: ... mouse GFP (11814460001, Roche, IF: 1/100); mouse HA (32-6700 ...