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Citations for Roche :
451 - 500 of 688 citations for M CSF Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: The rat pancreas was isolated following injection of 1 mg/mL collagenase P (Roche, Indianapolis, IN, USA) through the common bile duct ...
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bioRxiv - Microbiology 2019Quote: ... 1 h) and labeled with primary antibodies at the following dilutions: rat anti-HA (1:1000; Roche), mouse anti-Myc (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... The following antibody cocktails were used: rat anti-HA (1:200, Cat. No. 10145700, Roche Diagnostics, USA) with rabbit anti-CamKIIα (1:200 ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were incubated with 1:500 mouse anti-actin26 and 1:1000 rat anti-HA (3F10, Roche) followed by 1:1000 anti-mouse Alexa 488 (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... Following an overnight incubation at 4 °C with rat anti-HA (1:500, monoclonal, Roche, Cat # 11867423001) or mouse anti-GAPDH (1:25.000 ...
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bioRxiv - Cell Biology 2023Quote: Adult rat ventricular cardiomyocytes were freshly isolated by Langendorff perfusion with Liberase TM (0.225 mg/mL, Roche) in modified Krebs-Henseleit buffer (135mM NaCl ...
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bioRxiv - Microbiology 2023Quote: ... the polyclonal murine anti N-terminal MSPDBL2 serum and a rat monoclonal α-HA antibody (Roche 3F10) were used ...
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bioRxiv - Cell Biology 2024Quote: ... primary antibody incubation was performed in blocking buffer with anti-HA-tag (rat, Roche, 11867423001, 1:250) or anti-CD9 (mouse ...
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bioRxiv - Cancer Biology 2024Quote: ... Isolated hepatocytes were seeded at a density of 4-500,000 and 1,000,000 cells in rat tail collagen I (5 μg/cm2, Roche) pre-coated 6-well plates and T25 flasks ...
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bioRxiv - Biophysics 2021Quote: ... and then resuspended (6 mL buffer per 1 g of pellet) in the lysis buffer (50 mM HEPES, pH 7.5, 0.15 M NaCl, protease inhibitor cocktail (Roche), 1mM PMSF ...
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bioRxiv - Cell Biology 2020Quote: ... harvested cells were incubated with 25 μg/ml digitonin in buffer H (20 mM Hepes-KOH, pH 7.4, 0.25 M sucrose, 1 mM DTT, complete protease inhibitor cocktail [Roche] ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in 350 μl of lysis buffer (20 mM Tris pH 7.5, 0.5 M o NaCl, 1 mM EDTA, 1X protease inhibitor cocktail [Complete; Roche]) and 200 μl of glass beads (425-600 μm ...
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bioRxiv - Cell Biology 2022Quote: ... Pellets were resuspended in 120 μl lysis buffer (0.1 M NaOH, 0.05M EDTA, 2% SDS, 2% beta-mercaptoethanol, PhosStop (Roche), cOmplete protease inhibitor cocktail (Roche)) ...
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bioRxiv - Neuroscience 2019Quote: ... then immersed in 0.1 M maleate buffer (pH 7.5) for 10 minutes before blocking in 1% Blocking Reagent (Roche) for 10 minutes ...
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bioRxiv - Neuroscience 2020Quote: Hippocampi were collected 24 h after KA treatment and protein extracts were obtained by processing samples with lysis buffer (Tris-Base 0.5 M, pH 7.4; SDS 10 %) containing phosphatase and proteinase inhibitors (Roche). Lysates were clarified by centrifugation at 14000 x g for 10 min and protein concentration was measured using the bicinchoninic acid assay ...
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bioRxiv - Neuroscience 2021Quote: Mice brain tissues were homogenized in lysis buffer (50 mM Tris pH 7.6, 0.15 M NaCl and Complete protease inhibitor cocktail (Roche)) 11 ...
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bioRxiv - Molecular Biology 2020Quote: Frozen mouse tissues were homogenized in CelLytic M cell lysis reagent (MilliporeSigma) with cOmplete™ protease inhibitors (Roche). Tissue lysates were centrifuged at 10,000 × g for 5 min at 4 °C and supernatants were collected for further testing ...
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bioRxiv - Plant Biology 2021Quote: ... and 0.33 M D-sorbitol supplemented with one tablet (per 50 mL) of cOmplete protease inhibitor cocktail (Roche)] using a Waring blender ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in 350 μl of lysis buffer (20 mM Tris, pH 7.5, 0.5 M NaCl, 1 mM EDTA, 1x protease inhibitor cocktail [Complete; Roche]) and 200 μl of glass beads (425-600 μm ...
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bioRxiv - Cell Biology 2022Quote: ... The sonicated sample was layered over 3 ml of S3 solution (0.88 M sucrose, 0.5 mM MgCl2, 1x Complete protease inhibitor cocktail (Roche)) in a new Falcon tube and centrifuged at 3000 × g for 10 min at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... The resuspended pellet was layered carefully over 3 ml of S2 solution (0.35 M sucrose, 0.5 mM MgCl2, 1x Complete protease inhibitor cocktail (Roche)) and centrifuged at 1430 × g for 5 min at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... cells pellet was resuspended in 2 ml of lysis buffer (20mM sodium phosphate, 0.5 M NaCl, 20 mM imidazole; pH 7.4 with a protease inhibitor tablet (Roche) per 1 g of wet cell pellets ...
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bioRxiv - Neuroscience 2020Quote: ... The tissue was homogenized in 100 μL of ice-cold 0.1 M PBS (pH 7.4) supplemented with a protease inhibitor cocktail (Roche). The homogenate was divided into two fractions for further total mRNA and protein extraction ...
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bioRxiv - Neuroscience 2019Quote: ... 12 strokes, 900 rpm) using homogenizer buffer (0.32 M Sucrose, 5 mM HEPES pH 7.4, Protease inhibitor cocktail (Roche)) ...
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bioRxiv - Neuroscience 2020Quote: ... 1 M NaCl and protease inhibitor cocktail (cOmplete, Mini, Roche; two tablets per 10 mL of lysis buffer). The lysate was sonicated before AHA-labeled proteins were covalently coupled to alkyne-tagged agarose beads using reagents provided in the kit ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was removed and the pellet was dissolved in 500 µl of ice-cold nuclear solubilization buffer (25 mM HEPES [pH 6.8], 1 M sorbitol, 0.5% NP-40, 250 μg/ml PMSF, cOmplete [EDTA-free protease inhibitor cocktail; Roche]). Following incubation for 30 min at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... 0.1 M PIPES-NaOH pH 6.9, 2 mM EGTA, 1 mM MgSO4, 0.1 mM EDTA, cOmplete protease inhibitors [Roche]) (5 min ...
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bioRxiv - Cancer Biology 2022Quote: ... resuspended in homogenization buffer (0.32 M sucrose, 5 mM HEPES pH 7.4, 1 mM EDTA) containing protease inhibitor Complete (Roche) and then homogenized by multiple passing through a 0.24 mm syringe.
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bioRxiv - Molecular Biology 2021Quote: Protein lysates were prepared from snap-frozen dorsal skin with urea buffer (8 M Urea, 40 mM Tris-HCl, 2.5 mM EDTA, pH 8.0) containing phosphatase (PhosSTOP, Roche) and protease inhibitors (cOmplete Protease Inhibitor Cocktail Tablet EDTA-free ...
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bioRxiv - Cell Biology 2020Quote: ... then resuspended in 4°C homogenization buffer (0.6 M sorbitol, 10 mM Tris pH 7.4, 1 mM EDTA, 1 mM PMSF, 2× Roche cOmplete protease inhibitor cocktail ...
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bioRxiv - Immunology 2020Quote: ... frozen iRBC pellets were thawed and lysed directly in ultra-pure water containing protease inhibitor mix M (Roche) and 1mM PMSF (Sigma-Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... at a maximum of 5g/tube in 5mL of X-vivo15 media containing 0.13U/m Liberase TL (Roche), 10U/mL DNase (benzonase nuclease ...
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bioRxiv - Immunology 2022Quote: ... Cells were pelleted by centrifugation and resuspended in lysis buffer (40 mM Tris-HCl pH 8.0, 0.3 M NaCl, cOmplete protease inhibitor (Roche), 5 μg/mL DNase I ...
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bioRxiv - Developmental Biology 2022Quote: ... Sections were re-fixed in 4% PFA for 5 min and incubated in 0.2 M HCl for 10 min at RT before treatment with tri-ethanolamine buffer (662.5 µl tri-ethanolamine, 112 µl 1 M HCl, 49.1 µl H2ODT, 125 µl acetic anhydride; Roche). Slides were preincubated at 65°C in hybridization buffer (50% formamide (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2022Quote: Pelleted cells were lysed in 500 μl 8 M urea (pH 8.0) with 0.2% Rapidgest (Waters) and protease inhibitors (Roche) using three 20-s sonication pulses in a VialTweeter (DrHielscher) ...
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bioRxiv - Cell Biology 2022Quote: ... the chromatin pellet was suspended with buffer B (20 mM Tris-HCl, pH 8.0, 0.5 M NaCl, 10 mM EDTA, and 1× complete protease inhibitor cocktail; Roche), and then mononucleosome was extracted ...
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bioRxiv - Cell Biology 2022Quote: ... 1.5 mM MgCl2, 0.34 M sucrose, 10% glycerol, 1 mM DTT, 50 mM sodium fluoride, protease inhibitors [Roche]). Triton X-100 (0.1% ...
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bioRxiv - Systems Biology 2023Quote: ... Cell pellets were resuspended in 400µL of ice-cold disruption buffer (PBS containing 10% Glycerol, 1mM MgSO4, Benzonase 0,05U/m, Roche complete cocktail EDTA free 1 tablet/10mL ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.2 g of powder was dissolved in 1.2 ml of microsome buffer (50 mM MOPS, 0.5 M sorbitol, 5 mM EGTA, complete protease inhibitor (Roche) at pH 7.6) ...
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bioRxiv - Immunology 2023Quote: ... Input DNA was obtained following the sample phenol-chloroform purification steps post overnight de-crosslinking of 10 μg of sonicated sample at 65 °C in the presence of 0.2 M NaCl and 10 μg/μl of RNase A and 0.8 μg/μl of Proteinase K (Roche). DNA was quantified with the Qubit dsDNA HS Assay Kit (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... 1.5 mM MgCl2, 0.34 M sucrose, 10% glycerol, 1 mM DTT, 50 mM sodium fluoride, protease inhibitors [Roche]). Triton X-100 (0.1% ...
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ENGRAILED-1 transcription factor has a paracrine neurotrophic activity on adult spinal α-motoneuronsbioRxiv - Neuroscience 2023Quote: ... 50 mM Tris-HCl pH8.0, 0.3 M NaCl, Triton X-100 1%, EDTA-free Complete Proteases inhibitor cocktail from Roche and 250U/ml of benzonase endonuclease from Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... 1.5 mM MgCl2, 0.34 M sucrose, 10% glycerol, 1 mM DTT, 50 mM sodium fluoride, protease inhibitors [Roche]). Then was added Triton X-100 at a final concentration of 0.1% and the cells were incubated for 5 min on ice ...
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bioRxiv - Microbiology 2023Quote: ... 5 mM MgCl2, 0.1 M EDTA, 10 % glycerol and 0.5 mM DTT, supplemented with a protease inhibitor cocktail from Roche) or Trap buffer (10 mM HEPES pH = 7.5 ...
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bioRxiv - Immunology 2024Quote: ... RNA was harvested by removal of culture medium and addition of 200 µl of 1 M DTT (Roche) supplement Kingfisher RNA lysis buffer (Thermofisher) ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were harvested and resuspended in twice the cell pellet volume of lysis buffer (50 mM potassium phosphate pH 8.0, 0.5 M NaCl, 20 mM Imidazole, cOmplete protease inhibitor cocktail (Roche)) ...
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bioRxiv - Biochemistry 2021Quote: Rat total RNA was prepared from 100 mg of kidneys with the use of TriPure reagent (Roche, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... permeabilized and immunostained with the following primary antibodies - rat monoclonal anti-HA (clone 3F10, Roche: 1:250 dilution), mouse monoclonal anti-myc (clone 9B11 ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were incubated in PEMBALG containing monoclonal anti-HA antibody produced in rat (1:1000 dilution, Roche) for one hour ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then incubated with 40 µL of primary antibody (1:1,000 rat anti-HA mAb 3F10, Roche) at 4°C overnight in a solution containing 3% BSA in PBS ...