Labshake search
Citations for Roche :
451 - 500 of 1626 citations for 6 ethoxy 7H purin 2 amine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: ... SHIV-Ag85B was prepared by transfecting pSHIV-Ag85B into 293T cells using FuGENE 6 Transfection Reagent (Roche Diagnostics, Indianapolis, IN), and the culture supernatant was stored at 48 h after transfection in liquid nitrogen until use.
-
bioRxiv - Molecular Biology 2022Quote: ... The reaction was set by using qPCRBIO Probe Mix Hi-ROX (Nippongenetics) and TaqMan (5’: 6-FAM, 3’: TAMRA) or UPL (Universal Probe Library, Roche) probes ...
-
bioRxiv - Cancer Biology 2022Quote: A vector control plasmid and WT and mutant Plk3 expression plasmids were transfected into 293T cells using FuGENE 6 transfection reagent (Roche) as described previously (Li et al. ...
-
bioRxiv - Plant Biology 2022Quote: ... Glucose was then quantified by measuring NADH production using an assay based on hexokinase and glucose-6-phosphate dehydrogenase (Roche). Starch content was calculated in glucose equivalents.
-
bioRxiv - Developmental Biology 2022Quote: ... bleached with 6% hydrogen peroxide in PBS for 1h and treated with 10 μg/ml Proteinase K (Roche, Mannheim, Germany) for 20 min (5 min for epithelia ...
-
bioRxiv - Immunology 2023Quote: ... 1 mM EDTA) and then detected with 2,2’-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium (ABTS) substrate (Roche Diagnostics) diluted at 1:1000 in H2O2 ...
-
bioRxiv - Immunology 2023Quote: ... 1 mM EDTA) and then detected with 2,2’-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium (ABTS) substrate (Roche Diagnostics) diluted at 1:1000 in H2O2 ...
-
bioRxiv - Microbiology 2023Quote: ... Then the different pellets were resuspended for 30 min in denaturing buffer (20 mM Tris, 500 mM NaCl, 6 M Guanidine, 20 mM Imidazole and protease inhibitor cocktail (Roche), pH 7.5) ...
-
bioRxiv - Cell Biology 2023Quote: ... D-glucose was measured spectrophotometrically3 by reading the increase in NADPH(H+) absorbance at 340 nm produced in two coupled reactions catalyzed by hexokinase and glucose-6-phosphate dehydrogenase (G6PD) (Roche diagnostics Corporation ...
-
bioRxiv - Molecular Biology 2023Quote: For nuclear cell lysis 5×10^6 cells per condition were resuspended in 1mL of Lysis Buffer + protease inhibitor (cOmplete, Roche) (10mM Tris-HCl pH 7.4 ...
-
bioRxiv - Immunology 2023Quote: HEK293 cells were seeded at 400,000 cells per well in 6-well plates and transfected the day after with indicated cDNA constructs with FuGENE6 (Roche). Reverse transfection of siRNAs was carried out using RNAiMAX according to the manufacturer’s instructions (Invitrogen Thermo Fisher Scientific) ...
-
bioRxiv - Immunology 2023Quote: HEK293 cells were seeded 72 hours prior to the experiment at 400,000 cells per well in 6-well plates and transfected the next day with wt or mutated myc-ALPK1 constructs with FuGENE6 (Roche) according to manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2022Quote: Proteins were harvested from ∼30 mg of tissue (in vivo) or two 6-well plates (in vitro) and homogenised in Ripa buffer with complete mini-proteinase inhibitors (Roche). Preparation of nuclear and cytoplasmic extracts from human fibroblasts was performed using a NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher) ...
-
bioRxiv - Developmental Biology 2023Quote: ... Embryos were washed 6 times with MABT and were then incubated with anti-DIG AP-conjugated Fab fragment (Roche, 11093274910), Alexa-Fluor 488 goat anti-Mouse IgG1 antibody (ThermoFisher A-21121 ...
-
bioRxiv - Biochemistry 2023Quote: ... values of corresponding cDNAs with the QuantStudio 6 Pro system (Applied Biosciences, A43180) and FastStart Essential DNA Green Master (Roche) at a final concentration of 1X ...
-
bioRxiv - Cancer Biology 2023Quote: ... pUMVC for retrovirus) and 250 ng envelope vector (pCMV-VSV-G) are mixed with 20 µL Fugene 6 (Roche, USA) diluted in OptiMEM ...
-
Bni5 tethers myosin-II to septins to enhance retrograde actin flow and the robustness of cytokinesisbioRxiv - Cell Biology 2023Quote: ... Cells were then lysed by sonication 6 times for 15 sec each in one of the following buffers each containing the Protease Inhibitors Cocktail tablets (Roche) and 1 mg/mL lysozyme (L6876 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Libraries were amplified by ligation-mediated PCR for 6 cycles using a KAPA HiFi HotStart high-fidelity DNA polymerase (Roche) and purified using AMPure XP beads.
-
bioRxiv - Pathology 2023Quote: ... in HEK 293T cells (3×105 cells per well in 6-well plates) using X-treme gene transfection reagent (Roche). Pseudotyping was achieved by co-transfecting pHEF-VSVg (400 ng/well) ...
-
bioRxiv - Neuroscience 2024Quote: ... proteins were extracted by resuspending cells from 6-well plates in 100 μl of RIPA or 7M Urea Buffer containing protease inhibitor (11873580001, Roche) and ...
-
bioRxiv - Molecular Biology 2023Quote: ... a total of 0.4–0.5 μg of plasmid and dsRNAs were transfected into BmN4 cells (4–6 × 104 cells per glass bottom 35 mm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche) and cells were fixed 5–6 days later ...
-
bioRxiv - Microbiology 2024Quote: ... spleens were collected from naïve C57BL/6 (CD45.1+) and a single cell suspension was obtained by incubation with Liberase Blendzyme 3 (Roche, Bradford, CT) and DNase (Sigma ...
-
bioRxiv - Microbiology 2024Quote: ... Typhi harboring the different transcriptional reporters were grown at 37 °C in LB supplemented with glucose-6-phosphate (G6P) (Roche) (concentrations ranging from 7.6 μM to 10 mM ...
-
bioRxiv - Molecular Biology 2024Quote: ... Ovaries were cut into 6-8 pieces and enzymatically digested in L-15 media containing Liberase TM (Roche, Indianapolis, IN) and DNase I (Worthington Biochemicals ...
-
bioRxiv - Cancer Biology 2024Quote: ... PDOs were pooled from 6 wells and incubated for 1.5 hours on ice in Cell Recovery Buffer supplemented with phosphatase inhibitors (Roche, 4906845001). PDOs were lysed using RIPA buffer (Boston BioProducts ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cells were transfected with 1 µg of shRNA/cDNA and 1 µg of viral packaging plasmids (250 ng pMD2.G and 750 ng psPAX2) using 6 µl of Xtreme Gene 9 transfection reagent (Roche). After 24 hours of transfection ...
-
bioRxiv - Molecular Biology 2023Quote: ... Transfection of 106 cells in a 6-well plate was performed using 5 μl of transfection agent (X-tremeGENE HP Reagent, Roche), 10 μL of bacmid and 85 μL of SF900II-SFM medium (Gibco) ...
-
bioRxiv - Biochemistry 2021Quote: Cardiac myofibrils were prepared from left ventricular trabecular strips pre-stretched overnight to a sarcomere length of about 2 µm in rigor buffer (20 mM HEPES pH 7, 140 mM KCl, 2 mM MgCl2, 2 mM EGTA, 1 mM DTT, Roche complete protease inhibitor) at 4°C ...
-
bioRxiv - Genomics 2022Quote: ... Samples were ground to powder in liquid nitrogen and then dissolved in 2 mL lysis buffer (8 M urea, 2% SDS, 1x Protease Inhibitor Cocktail (Roche Ltd. Basel, Switzerland), followed by sonication on ice for 30 min and centrifugation at 13 000 rpm for 30 min at 4 °C ...
-
bioRxiv - Genomics 2021Quote: ... and then resuspended in Antibody buffer (10 mM HEPES pH 150 mM NaCl, 2 mM spermidine, 2 mM EDTA, 0.1% BSA, and Roche complete EDTA-free protease inhibitor) with primary anti-Pol2S5p antibody (Cell Signaling Technology cat ...
-
Polo-like kinase 1 independently controls microtubule-nucleating capacity and size of the centrosomebioRxiv - Cell Biology 2020Quote: ... 2 mM MgCl2 and EDTA-free protease inhibitor cocktail (Roche)) and lysed in an ice-cold sonicating water bath for 5 minutes ...
-
bioRxiv - Neuroscience 2020Quote: ... 25 μl of 2× HIFI HotStart Ready Mix (Kapa Biosystems), and 0.2 M SMART PCR Primer and polymerase chain reaction (PCR ...
-
bioRxiv - Molecular Biology 2019Quote: ... PMSF with 2 cOmplete protease inhibitor cocktail tablets (Roche 04693116001) per 50 mL of buffer) ...
-
bioRxiv - Molecular Biology 2021Quote: ... 2 μg/ml RNase A and protease inhibitor mixture (Roche). Proteins were bound to GST resin (GE Healthcare ...
-
bioRxiv - Molecular Biology 2020Quote: ... 2 μg/ml Rnase A and protease inhibitor tablets (Roche)) ...
-
bioRxiv - Molecular Biology 2019Quote: ... 2 mM TCEP and a cocktail of protease inhibitors (Roche) using a syringe fitted with a 26G ½ needle ...
-
bioRxiv - Immunology 2019Quote: ... supplemented with 2% FCS and 1mg/mL dispase II (Roche) overnight at 4°C ...
-
bioRxiv - Systems Biology 2019Quote: ... 2% SDS and protease inhibitors (complete mini., EDTA-free; Roche). Cells were lysed by sonication ...
-
bioRxiv - Genomics 2021Quote: ... 0.0075% DIG and 2 U/ul Protector RNase inhibitor (Roche)) for 1 min on ice ...
-
bioRxiv - Developmental Biology 2020Quote: ... The embryos were blocked with 2% Blocking Reagent (Roche, Mannheim) for 1h ...
-
bioRxiv - Neuroscience 2020Quote: ... and 10 ng/mL fibroblast growth factor 2 (FGF2, Roche). Oligodendrocyte precursor cells were removed by brusquely shaking the culture flasks several times when changing the medium after 2 or 3 days ...
-
bioRxiv - Immunology 2019Quote: ... and 60 U/mL human IL-2 (Proleukin, Roche Diagnostics). The A20 cells were grown in RPMI-1640 (Gibco ...
-
bioRxiv - Cell Biology 2021Quote: ... 1% Triton X-100) with 2% complete protease inhibitor (Roche) for 15 min on ice ...
-
bioRxiv - Neuroscience 2021Quote: ... and a phosphatase inhibitor cocktail (2×PhosSTOP from Roche Diagnostics). Proteins were quantified using the Bio-Rad DC Protein assay (Bio-Rad) ...
-
bioRxiv - Cancer Biology 2021Quote: ... 2% SDS) in the presence of cOmplete and PhosSTOP (Roche). Protein-containing supernatants were pre-cleared for 1 h at 4°C with Dynabead® protein G (Thermo Fisher Scientific) ...
-
bioRxiv - Cancer Biology 2020Quote: ... and 60 U/mL human IL-2 (Proleukin, Roche Diagnostics). The F9 teratocarcinoma cells were grown in DMEM (Gibco ...
-
bioRxiv - Cell Biology 2020Quote: ... 0.1% 2-mercaptoethanol) in the presence of protease inhibitors (Roche) and phosphatase inhibitors (1 mM sodium orthovanadate ...
-
bioRxiv - Cell Biology 2021Quote: ... PMSF (2 mm) and Protease Inhibitor Tablet (Roche, Basel, Switzerland), RNase A (1 mg·mL−1 ...
-
bioRxiv - Cell Biology 2021Quote: ... PMSF (2 mm) and Protease Inhibitor Tablet (Roche, Basel, Switzerland), RNase A (1 mg·mL−1 ...
-
bioRxiv - Neuroscience 2022Quote: ... 2 mM EDTA pH 8.0) containing Protease Inhibitor Cocktail (Roche). Nuclei were lysed using a Misonix XL-2000 Probe Sonicator with 5-10 second ON / 20 second OFF intervals until the lysate was clear (2 rounds for P0 ...