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Citations for Roche :
451 - 500 of 2344 citations for 3 4 Dimethyl 2' 4 methylpiperazinomethyl benzophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Transfected DRG neurons were fixed 15 min in 4 % paraformaldehyde (PFA) and hybridized with DIG-labeled GFP cRNA probes (Roche) as described previously (Merianda et al. ...
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Elevated Hoxb5b expands vagal neural crest pool and blocks enteric neuronal development in zebrafishbioRxiv - Developmental Biology 2021Quote: ... Probed embryos were blocked for 1-2 hours at ambient temperature in 5% Goat sera in PBST before detection overnight (∼16 hours) at 4°C using an anti-Digoxigenin-Fab fragments conjugated to Alkaline Phosphatase enzymes (1:1000 dilution, Roche) in 5% Goat Sera in PBST ...
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bioRxiv - Bioengineering 2021Quote: Cell-seeded scaffolds were collected at weeks 0 and 4 and digested in proteinase K solution (0.5 mg/ml in phosphate buffer, pH 6.5, Roche, Basel, Switzerland) for 48 hours at 56 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were hybridized with antisense RNA probe for IR84a (nucleotides 82-780) or IR8a (nucleotides 4-529) sequence labelled using in vitro transcription with digoxigenin-11-UTP (Roche). The signals were visualized with a nonradioactive detection system using anti-digoxigenin Fab fragment conjugated to alkaline phosphatase (Roche).
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bioRxiv - Neuroscience 2021Quote: ... the lysates were centrifuged (10 min, 20000 g, 4 °C) and the resulting supernatants were added to 25 µl Protein G Agarose (Roche), rotating for 1 hour at 4 °C ...
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bioRxiv - Pathology 2021Quote: ... and homogenized to 20% w/v in PMCA I buffer (1x PBS 150 mM NaCl, 4 mM EDTA) with cOmplete protease inhibitor (Roche). Samples were homogenized on a BeadBlaster for 10 rounds ...
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bioRxiv - Cancer Biology 2020Quote: ... were co-transfected with 2ug of the Cas9/sgRNA vector PX458 and 4 ul of ssODN HDR template (20 uM) using Xtreme gene (Roche). Two days following transfection ...
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bioRxiv - Biochemistry 2021Quote: ... Then qPCR was performed in triplicate using 4 μl sample in 8-9 μl reaction with FastStart Universal SYBR Green Master Mix (Roche) and primers flanking each MseI cassette (Table S3).
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bioRxiv - Cell Biology 2021Quote: ... 500 mM NaCl, 4 mM β-mercaptoethanol, 10 mM ATP, 2.5 mM MgCl2, cOmplete EDTA-free protease inhibitor cocktail-Roche). The beads were eluted with NTA elution buffer (1x PBS ...
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bioRxiv - Cell Biology 2021Quote: ... the sections were incubated overnight at 4 °C in digoxigenin antibodies conjugated to horseradish peroxidase (anti-digoxigenin-POD; Fab fragment; 1:100; Roche), rinsed in TBS (0.1 M Tris-HCl with 0.9% NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... and RNAseq libraries (2 biological replicates per condition comprised of a pool of 4 PA-gel cultures each) prepared using KAPA mRNA HyperPrep Kit (Roche) and IDT dual indexed sequencing adaptors ...
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bioRxiv - Cell Biology 2022Quote: All controls contained 4% (v/v) 25x concentrated protease inhibitor cocktail solution (cOmplete Protease inhibitor cocktail tablets, Roche, Mannheim, Germany) in accordance with the PPP samples.
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bioRxiv - Cell Biology 2022Quote: ... washed with PBS and resuspended in lysis buffer (0.5% Triton X-100, 4% w/v SDS, 0.5xPBS) supplemented with protease inhibitor cocktail (Roche, Basel, Switzerland). The lysates were then resuspended in 5 x SDS-PAGE loading buffer containing 25mM DTT ...
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SARS-CoV-2 Point Mutation and Deletion Spectra, and Their Association with Different Disease OutcomebioRxiv - Microbiology 2022Quote: PCR products were adjusted to 4 × 109 molecules/μl before generating DNA pools that were purified using Kapa Pure Beads (Kapabiosystems, Roche), and quantified using Qubit as previously described (38–40) ...
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bioRxiv - Neuroscience 2021Quote: ... 0.4 mM phenylmethylsulfonyl fluoride (PMSF) and 4 µM pepstatin) and digested with the chosen endoglycosidase (PNGase F, New England Biolabs; or Endoglycosidase H, Roche) in a small volume of the appropriate buffer ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were incubated overnight at +4 °C with the antibody anti-digoxigenin-AP Fab fragment (#11093274910, Roche, 1:1000) diluted in blocking solution in a humidity chamber ...
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bioRxiv - Neuroscience 2020Quote: ... cells were washed once with room temperature PBS and then lysed in a solution of 1 volume 5x SDS-PAGE sample buffer (containing fresh 5% (v/v) beta-mercaptoethanol) to 4 volumes of Immunoprecipitation buffer (IPB; (Thomas et al. 2012)) containing fresh Protease Inhibitor Cocktail (Roche) and 1 μM microcystin-LR ...
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bioRxiv - Neuroscience 2020Quote: ... Blots were then incubated overnight at 4 °C with primary antibody of interest diluted in blocking solution (Western Blotting Reagent, Roche). The appropriate species of HRP-conjugated secondary antibody (from Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated overnight at 4°C with primary antibody for rat anti-HA (1:200; 12013819001, RRID:AB_390917, Roche, Basel, Switzerland), rabbit anti-GFP (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... This was spun down (5 minutes at 1800 RMP, 4°C) and the pellet then digested in 10ml collagenase/dispase (1mg/ml; Roche) and DNase I type IV (40µg/ml ...
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bioRxiv - Cell Biology 2022Quote: ... incubation at 4 °C rotating for 3 h, followed by incubation (4 °C, rotating) with 30 μl of pre-washed Protein A agarose bead slurry (#11134515001, Roche) for an additional hour ...
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bioRxiv - Pathology 2020Quote: Lungs harvested and cut into small pieces were digested (1h, 37c) with 4 mg/ml collagenase D (Roche, Mannheim, Germany). Tissue was meshed through a 40-µm cell strainer ...
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bioRxiv - Pathology 2020Quote: ... Tween-20 0.1%) for 1 hour and incubated overnight at 4 °C with the appropriated antibodies: HA (dilution 1:1000, Roche, #867423001), ß-tubulin (dilution 1:5000 ...
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bioRxiv - Developmental Biology 2021Quote: ... 2% sheep serum in PBST) for one hour and overnight at 4 °C with alkaline phosphatase-conjugated anti-DIG antibodies (Roche) diluted at 1:4000 in the blocking solution ...
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bioRxiv - Molecular Biology 2021Quote: Embryo extracts were pre-cleared with empty beads equilibrated in IPP 150 for 30 min at 4°C in the presence of 1x complete protease inhibitor cocktail (Roche). Pre-cleared extracts (300-500 µg ...
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bioRxiv - Microbiology 2021Quote: Wildtype genes were amplified by PCR from JE2 genomic DNA using the primers shown in table 4 and KAPA HiFi polymerase (Roche). The PCR product was cloned into the tetracycline inducible plasmid pRMC2 using KpnI and SacI restriction sites and T4 DNA ligase (NEB) ...
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bioRxiv - Cell Biology 2021Quote: Human pluripotent stem cells (hPSCs) were maintained in E8 medium and passaged every 4 days onto matrigel-coated plates (Roche). The following hPSC lines were used in the study ...
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bioRxiv - Cell Biology 2020Quote: ... Pelleted tissue was weighted and resuspended in the enzymatic digestion mix composed by 2,4 U/ml dispase II (4 ml/g of muscles) (Roche 04942078001) dissolved in Dulbecco’s phosphate buffered saline (D-PBS ...
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bioRxiv - Immunology 2020Quote: ... Bacterial pellets obtained by centrifugation at 13,500g for 10 min at 4°C were resuspended in 1 ml of PBS containing protease inhibitors (cOmplete Mini, Roche; 1 tablet per 10 ml of PBS following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The samples were subsequently diluted by adding 150 μL of water and 4 μL was used for RT-qPCR performed on a LightCycler96 platform (Roche) using the iTaq Universal Probes One-Step RT-qPCR kit (BioRad ...
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bioRxiv - Microbiology 2020Quote: ... coli cells were resuspended in 200 mL of cold Lysis buffer (50 mM HEPES pH 8, 300 mM NaCl, 1 mM EDTA, 5 % glycerol, 4 tablets of protease inhibitors, Roche). The cells were lysed and the membrane fraction was collected as described in the YukC purification protocol below ...
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bioRxiv - Developmental Biology 2020Quote: ... samples were incubated overnight at 4°C with an anti-DIG-AP antibody (Roche, diluted 1:2000 in blocking solution). The next day ...
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bioRxiv - Immunology 2021Quote: ... total cellular protein extracts were obtained by lysing cells for 30 min at 4°C in lysis buffer (Pierce RIPA Buffer) in the presence of protease inhibitors (cOmplete Protease Inhibitor Cocktail, ROCHE). Samples were centrifuged for 20 min at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... blocked with blocking solution for 1 hour and finally incubated overnight at 4°C with anti-Fluorescein-POD antibody (Roche) diluted 1:500 in blocking solution ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed once with PBS and resuspend with Tris-HCl containing 4 mM EDTA and 1× complete protease inhibitor cocktail (Roche). Dounce homogenizer is used to fractionate the cell membranes and then 3 min 600 g centrifugation was used to obtain Post-Nuclear Supernatants (PNS) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... in PBS for 30 min and incubated overnight at 4°C with 1:1,500 anti-HA high affinity (Roche, 3F10). After three PBS washes ...
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bioRxiv - Cell Biology 2023Quote: Cells were lysed in SDS lysis buffer (4% SDS, 20% glycerol, 125 mM Tris-HCl pH=6.8) supplemented with protease inhibitors (cOmplete ™ Mini (Roche)) ...
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bioRxiv - Neuroscience 2023Quote: Pools of 2 to 4 organoids were resuspended in cold lysis buffer (Cell Signalling Technology) supplemented with protease and phosphatase inhibitor cocktails (Roche) and sonicated twice for 3 seconds at 10% intensity in the Branson digital Sonifier SFX 150 (Emerson) ...
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bioRxiv - Molecular Biology 2022Quote: ... and lysed by 4 freeze/thaw cycles in the presence of 1mM PMSF and cOmplete protease inhibitor cocktail tablet (Roche). Lysates were clarified by centrifugation at 39,000xg for 1.5hrs ...
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bioRxiv - Molecular Biology 2022Quote: ... UV cross-linked (1200J/m2) and hybridized with a DIG-labelled (GCCTAA)4 probe at 37°C using DIG Easy Hyb (Roche) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2022Quote: ... dissociated with an equal mixture of 4 mg/ml of collagenase D and dispase II (Roche Applied Science, Indianapolis, IN) and cultured in F-12K (Corning ...
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bioRxiv - Molecular Biology 2022Quote: ... Sonicated samples were centrifuged and the supernatant (soluble fraction) was incubated for 1 h at 4°C with beads crosslinked to anti-GFP antibody (11814460001; Roche) or to M2 anti-FLAG antibody (F1804 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... centrifuged for 5 min at 1000 rpm and stored at 4 °C until measurement for LDH activity according to the manufacturers protocol (Roche, LDH Cytotoxicity Detection Kit ...
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bioRxiv - Neuroscience 2024Quote: ... Whole mount larvae and adult brain sections layered on glass slides were blocked for at least one hour in PBT with 2 mg/ml bovine serum albumin and 2% sheep serum at room temperature and then incubated overnight at 4°C with alkaline phosphatase-coupled anti-DIG antiserum (11093274910, Roche) diluted 1/5000 in blocking solution ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting supernatants were incubated for 16 h while rotating at 4°C with 8 µg/ml anti-GFP antibody (Roche). Protein A/G PLUS-Agarose (Santa Cruz ...
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bioRxiv - Molecular Biology 2024Quote: ... The structure of the lacO–tetO reporter locus was checked by a set of 4 PCRs using Expand Long Template PCR System (Roche) and the following primer pairs ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were washed and incubated overnight at 4 °C with an alkaline phosphatase-conjugated anti-digoxigenin antibody (1:2500-1:4000, Roche). To visualize the RNA-probe binding ...
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bioRxiv - Genomics 2023Quote: One hundred mg of 14-day-old seedlings were ground and nuclei were isolated with 4°C Buffer (0,25M Sucrose, 10mM Tris-HCl, 10mM MgCL2, 1% Triton, 5mM ß- mercaptoethanol) containing proteinase inhibitor cocktail (Roche) and filtered in 63 µm ...
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bioRxiv - Neuroscience 2023Quote: ... The larvae were then incubated overnight at 4°C with anti-Dig-AP (1:2000 in 5% normal goat serum, 11093274910, Roche) and washed before detecting alkaline phosphatase using NBP/BCIP(Roche ...
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bioRxiv - Immunology 2022Quote: ... and 11-week infected half brains and liver lobes of mice (n=4/group) was extracted and purified using a High Pure PCR Template Prep Kit (Roche). DNA concentration of each sample was determined via NanoDrop ...