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Citations for Merck :
151 - 200 of 686 citations for ssc mir 323 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: ... washed again 3 times 15 min and developed using Millipore Crescendo ECL (Merck). For western blot quantification ...
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bioRxiv - Developmental Biology 2021Quote: ... washed 3 times in PBS with 1x cOmplete Protease Inhibitor Cocktail (Merck, 11697498001).
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bioRxiv - Immunology 2020Quote: ... by filtrating four times over 100 kDa cut-off cellulose centrifugal filter (Merck).
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bioRxiv - Microbiology 2023Quote: ... Cells were washed four times with PBS and lysed with 2% saponin (Merck) for 30 minutes ...
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bioRxiv - Microbiology 2021Quote: ... PCR grade water (Lichrosolv®; Merck, Darmstadt, Germany). The qPCR was performed in triplicates using the CFX96 Touch™ Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... or Power SYBR Green PCR Master Mix (Merck). For all groups ...
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bioRxiv - Genomics 2023Quote: ... PCR reactions were conducted using KAPA Taq (Merck) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Detection of newly synthesized proteins was carried out by an anti-puromycin antibody (clone 12D10, mouse-monoclonal, MABE343; Merck), an anti-HuD antibody (ab96474 ...
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bioRxiv - Neuroscience 2022Quote: ... 50 μL/well of detection antibody (anti-FLAG-D2) diluted in Erenna Assay buffer (catalog #02-0474-00; Merck) at the final concentration of 1 μL/mL were added to the assay plate and incubated for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... The in situ proximity ligation assay (PLA) in combination with immunofluorescence microscopy was performed using the Duolink Detection (Merck) or the NaveniFlex (Navinci diagnostics ...
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bioRxiv - Cancer Biology 2023Quote: ... Detection by enhanced chemiluminescence was performed using the Immobilon Forte HRP Western substrate (ref WBLUF0500, Merck Millipore, Darmstadt, Germany) or SuperSignal West Femto Maximum Sensitivity Substrate (ref 34096 ...
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bioRxiv - Neuroscience 2023Quote: Primary antibodies used for the detection of the autophagic mechanisms by immunoblot were anti-LC3B antibody (Merck Group, L7543) and anti-SQSTM1/p62 (Merck Group ...
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bioRxiv - Cell Biology 2023Quote: ... Detection by enhanced chemiluminescence was performed using the Immobilon Forte HRP Western substrate (ref WBLUF0500, Merck Millipore, Darmstadt, Germany) or SuperSignal West Femto Maximum Sensitivity Substrate (ref 34096 ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were incubated for 1.5 hours at RT with an antibody against Hp1α (Merck Millipore, MAB3584, 1:500 dilution) and RNA Polymerase II (Santa Cruz ...
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bioRxiv - Neuroscience 2019Quote: ... Slides were air dried overnight in the dark at RT and cover-slipped using entellan-toluene solution (Merck Chemicals) the following day.
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bioRxiv - Microbiology 2021Quote: Cells grown on glass coverslips were fixed in 4% PFA for 30 min at RT and permeabilized with 0.2% Triton X-100 (Sigma/Merck) in PBS for 10 min at RT ...
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bioRxiv - Cell Biology 2022Quote: ... the nuclei were visualized with Hoechst 33258 (Merck, B2883, 2 µg/ml in H2O at RT for 5 min).
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bioRxiv - Neuroscience 2023Quote: ... 1c and Fig.3 were first fixed with 4% PFA solution for 30 minutes at RT and quenched with 0.1 M glycine (Merck) solution in PBS for 15 minutes at RT ...
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bioRxiv - Bioengineering 2023Quote: ... for 20 min at room temperature (RT) and permeabilised overnight at 4 °C in 0.5% Triton X-100 (Merck) solution in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... specimens were post-fixed for 2 h at RT in 1% osmium tetroxide in 0.05 M cacodylate buffer pH 7.4 (Merck, Germany) and dehydrated stepwise in a graded ethanol series followed by 100% acetone ...
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bioRxiv - Neuroscience 2024Quote: ... and then blocked for 1 h at RT with 0.2% Triton-X in PBS + 1% normal goat serum (NGS, Merck). Antibodies used and their concentration is found in Supplementary Table 4 ...
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bioRxiv - Cell Biology 2024Quote: ... The immunostainings were performed at RT using tris-buffered saline (TBS) containing 1.6% (v/v) fish gelatine (G7765, Merck) as the basal buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were clarified by centrifugation (10,000 x g, 30 min, RT) and incubated for 1 hour with Ni-NTA His bind resin (Merck) rolling at RT ...
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bioRxiv - Cancer Biology 2020Quote: ... slides were washed with deionized water three times and counterstained with Mayer’s hematoxylin (Merck). Slides were then rinsed with deionized water for 5 minutes and mounted with a coverslip using Aquatex mounting medium ...
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bioRxiv - Neuroscience 2022Quote: ... slices were washed two times with 1X Phosphate Buffer (30 mM Na2HPO4·12H2O [Merck, 10039-32-4] ...
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bioRxiv - Developmental Biology 2022Quote: ... and detergent were removed by washing five times with 8M Urea (Merck, Darmstadt, Germany) 50mM Tris (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2021Quote: ... Wells were washed 3 times with 200 µL PBS containing 0.1% Tween-20 (Merck) (PBS-T ...
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bioRxiv - Cell Biology 2021Quote: ... Coverslips were washed three times with PBS and mounted in Moviol 4-88 (Merck). Wide-field fluorescence microscope Leica (Leica ...
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bioRxiv - Molecular Biology 2020Quote: ... Fresh fecal samples were diluted with nine times buffered peptone water (BPW) (Merck, Germany) and 25 g of sample was added to 225 mL of BPW pre-enrichment media ...
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bioRxiv - Cell Biology 2022Quote: ... passed 10 times though a 27 G needle and laid on an Optiprep (Merck) gradient ...
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bioRxiv - Developmental Biology 2023Quote: ... and detergent were removed by washing five times with 8M Urea (Merck, Darmstadt, Germany) 50mM Tris (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2023Quote: ... We dipped shoots 20 times in a 0.1% Tween 20 solution (Merck, NJ, USA) followed by 20 rinses with milli-Q water to remove surface trace element contamination (Schmidt et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... Secondary antibodies fused to HRP were used for detection (Goat anti-mouse HRP 1:3000, BioRad; Goat anti-rat HRP 1:3000, Merck Millipore ...
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bioRxiv - Microbiology 2020Quote: ... A was 1 mM ammonium formate pH 9 (for lincomycin detection, prepared by titration of formic acid 98–100%, Merck, Germany with ammonium hydroxide 28–30% ...
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bioRxiv - Microbiology 2020Quote: ... for ELISpot detection in combination with multiscreen immunoprecipitation (IP) ELISpot polyvinylidene difluoride (PVDF) 96-well plates (Merck Millipore, Darmstadt, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were washed 3x with TBS-T and subjected to chemiluminescence detection with Immobilon Western Chemiluminescent HRP substrate (Merck Millipore) using a Gel-Doc XR+ System (Bio-Rad) ...
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bioRxiv - Immunology 2021Quote: ... was mixed 1:1 and incubated at RT for 2-3 min or ECL substrate is added (Immobilon crescendo western HRP substrate, WBLUR0100, Merck). Membranes were then exposed to film and developed or visualised by chemiluminescence using the G:BOX Chemi gel doc Imaging System Instrument (Syngene) ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were permeabilized with 0.1 % Triton-X100 in PBS for 20 min at RT and samples were blocked with freshly prepared 3 % bovine serum albumin (BSA, Merck) in PBS for 1 h at RT ...
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bioRxiv - Cell Biology 2022Quote: ... for 1 h at room temperature (RT) and revealed by chemiluminescence using Immobilon Crescendo or Forte Western HRP substrate (Millipore Merck).
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bioRxiv - Cell Biology 2020Quote: ... MiRNA in situ hybridization was performed in formaldehyde and carbodiimide (EDC)-fixed TA cryo-sections (0,16M 90 min at RT, #25952-53-8, Merck KGaA). After washes with 0,2% glycine (#G8898 ...
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bioRxiv - Cell Biology 2020Quote: ... alkylated with 40 mM Chloroacetamide (CAA) at RT for 30 min and protein amount was quantified using the Direct Detect Spectrometer from Merck. Protein digestion was performed using the Single-Pot Solid-Phase-enhanced Sample Preparation approach SP3 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were fixed with 4% (vol/vol) paraformaldehyde for 10 min at RT and permeabilized with 0.3% Tween 20 (#822184, Merck Millipore). All the primary and secondary antibodies were diluted in blocking buffer consisting of 0.3 M glycine ...
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bioRxiv - Immunology 2020Quote: ... The sections were blocked in PBS containing 1% bovine serum albumin (BSA) for 1 h at RT and stained in blocking buffer containing primary antibody (anti-PP6C, Merck Millipore cat ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed and stained with indicated primary antibodies for 2 h at RT followed by incubation with PLA probes (Merck, Duolink® In Situ PLA® Probe Anti-Rabbit PLUS ...
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bioRxiv - Immunology 2022Quote: ... the sections were treated with 10% methanol in PB for 20 min and then incubated for 1 h at RT in incubation buffer: 5% normal donkey serum (NDS: Merck) and 0.2% Triton X-100 (Merck ...
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bioRxiv - Neuroscience 2023Quote: ... Plates were washed three times with 0.1% PBST and incubated at RT for 1 hour with horseradish peroxidase-conjugated secondary antibody (Cat No. NA934, Merck Millipore) diluted 1:1,000 in 0.05% PBST ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were incubated for 1 h at RT with a mouse anti-2A primary antibody (cat. no. MABS2005, Merck) diluted 1:2000 in 1% (w/v ...
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bioRxiv - Developmental Biology 2023Quote: ... Haematoxylin and Eosin staining was performed on few ST muscle sections by incubating the slides at RT for 1 min in hematoxylin solution (Merck). Slides were then washed for 10 min in running tap water before incubating for 1 min in eosin solution (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... in Tris- buffered salione containing 0.1% Tween-20 (v/v) (TBST) fore 60 mins at RT and incubated overnight with primary antibody against REST (1:500) (07-579, Merck), H3K9me2 (1:500 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were fixed using formaldehyde (1% V/V in H2O) for 10 min at RT before supplementation with 125 mM glycine solution (Merck) and agitation for 5 min ...