Labshake search
Citations for Merck :
251 - 300 of 686 citations for rno mir 137 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2020Quote: ... media were harvested and concentrated by 20 times using Amicon Ultra-15 Centrifugal Filter Units (Ultracel-10K, Merck).
-
bioRxiv - Molecular Biology 2022Quote: Time-lapse fluorescence microscopy (TLFM) was performed on B04A plates with an ONIX flow control system (Merck-Millipore) (24 ...
-
bioRxiv - Cell Biology 2022Quote: ... and concentrated at 4 °C approximately 300 times using a 10 kDa Centricon Plus-70 centrifugal unit (Merck Millipore ...
-
bioRxiv - Cell Biology 2022Quote: ... Pooled eluates were then concentrated approximately 40 times using a centrifugal filter (Amicon Ultra-2 10k, UFC201024, Merck Millipore ...
-
bioRxiv - Immunology 2023Quote: ... The samples were then rinsed two times with 0.2% PBT and mounted in Fluorsave (FluorSave Reagent 345789, Merck). The samples were visualized with a conventional fluorescent microscope (BX53 ...
-
bioRxiv - Molecular Biology 2023Quote: ... This protein sample was concentrated 10 times using a 3.5 kDa Amicon Ultra 15 mL device (#UFC500324, Merck) at 3,000 x g 80 minutes ...
-
bioRxiv - Microbiology 2023Quote: ... centrifuged 5 min at 2000rpm and concentrated 10 times using Amicon® Ultra 15 mL Filters (Merck #UFC910096). The concentrated virus aliquoted and stored at -80°C.
-
bioRxiv - Biophysics 2023Quote: ... the vesicle suspension was extruded 31 times through a Whatman Nucleopore™ track-etched polycarbonate membrane (Merck, Germany) with 200 nm pore size using a Mini-Extruder (Avanti Polar Lipids ...
-
bioRxiv - Developmental Biology 2024Quote: ... Brains were rinsed three times for at least ten minutes in a 0.1% DMSO and 0.1% Triton X-100 (Merck) PBS 1X solution (PBT ...
-
bioRxiv - Microbiology 2024Quote: ... Membranes were washed 3 times with PBS-T for 5 min and incubated with mouse-HRP (A4416; Merck) and rabbit-HRP (GENA9640V ...
-
bioRxiv - Genomics 2021Quote: ... we added 15μL PCR mix containing 12.5μL KAPA HiFi HS mastermix (Merck), 0.25μL 10μM Smart-seq2 ISPCR primer (SigmaAldrich ...
-
bioRxiv - Synthetic Biology 2021Quote: ... PCR amplification was performed by KOD Hot Start DNA polymerase (Merck Millipore). All enzymes ...
-
bioRxiv - Synthetic Biology 2021Quote: ... PCR amplifications were performed using KOD Hot Start DNA polymerase (Merck Millipore). All restriction enzymes ...
-
bioRxiv - Biophysics 2019Quote: ... coli MG1655 genome by PCR into pET15b (Merck Millipore, Billerica, MA, USA) by Gibson assembly (New England Biolabs ...
-
bioRxiv - Synthetic Biology 2023Quote: ... PCR reactions were performed using the KOD Hotstart DNA polymerase (Merck Millipore). Plasmid isolation ...
-
bioRxiv - Synthetic Biology 2024Quote: ... was used PCR reactions were performed using KAPA2G Fast Hotstart Readymix (Merck) and purified using Monarch® PCR & DNA Cleanup Kit (New England Biolabs) ...
-
bioRxiv - Developmental Biology 2021Quote: ... after which they were washed three times in PBST and blocked overnight at 4°C in 10% serum (Merck) in PBS/0.3% TritonX100 ...
-
bioRxiv - Immunology 2021Quote: ... The plates were then washed 5 times with HSWB followed by incubation of goat anti-mouse antibody (Merck, Germany) conjugated with HRP (diluted 1:3000 in LSWB + 1% BSA ...
-
bioRxiv - Cell Biology 2019Quote: ... Gels were then washed three times with PBS and coated with either 10 µg/ml fibronectin (Merck-Millipore, 341631) (U2OS and U-251 cells ...
-
bioRxiv - Cell Biology 2021Quote: ... Then cells were washed 3 times with deionized water (Milli-Q® IQ 7003 Ultrapure Lab Water System, Merck), and dehydrated by washing them in a graded series of ethanol ...
-
bioRxiv - Plant Biology 2021Quote: ... NaOCl and 0.02% (v/v) Triton X-100 for 10 min and washed five times with ultrapure water (Milli-Q; Merck). Following seed stratification at 4°C for 2 d ...
-
bioRxiv - Systems Biology 2020Quote: ... the cells were washed three times with PBS and saved in PBS supplemented with 0.02% sodium azide (Merck, S2002) at 4°C until image acquisition.
-
bioRxiv - Cell Biology 2022Quote: ... On the next day the membranes were washed three times for tenminutes in Tris-buffered saline + 0.05% Tween (#91414, Merck) followed by incubation with IRDye Goat anti-Mouse 800CW (#926-32210 ...
-
bioRxiv - Biochemistry 2019Quote: ... Samples were washed three times with 1X PBS and IgG was eluted using 0.1M formic acid (Merck, Darmstadt, Germany) followed by immediate neutralisation with 1M ammonium bicarbonate (Acros Organics ...
-
bioRxiv - Immunology 2020Quote: ... the reduced peptide solution was purified five times using ZipTip Pipette Tips with C18 resin and 0.6 µl bed volume (Merck,) and eluted in 32.5% ACN/0.2% TFA ...
-
bioRxiv - Cell Biology 2021Quote: ... the cells were washed three times with PBS and for 1sec preextracted using PBS supplemented with 0.1% (v/v) Triton-X100 (Merck) to reduce the nucleoplasmic EGFP signals ...
-
bioRxiv - Immunology 2022Quote: ... samples were re-loaded four times before washing with 1 mL of 2% acetonitrile (ACN) (#1000292500, Sigma-Aldrich, Merck) in 0.2% acetic acid ...
-
bioRxiv - Bioengineering 2022Quote: ... and washed four times in citrate-PBS supplemented with 0.01% bovine serum albumin (BSA, 10735086001, Merck KGaA, Darmstadt, Germany). PBMCs were frozen at 105 cells/ml in freezing medium containing RPMI-1640 (RPMI ...
-
bioRxiv - Biophysics 2023Quote: ... The suspension was extruded 31 times through a polycarbonate membrane (Whatman® Nuclepore™ Track-Etched Membranes, Merck, Germany) with pore size of 200 nm using a mini-extruder (Avanti Polar Lipids).
-
bioRxiv - Cell Biology 2023Quote: ... The samples were washed three times in 200 µL of the same buffer in a 30-kDa Amicon Ultra 0.5-mL centrifugal filter (Merck) to remove free ubiquitin ...
-
bioRxiv - Biophysics 2023Quote: Glass cover slides were washed several times with isopropanol and ultrapure water (18.2 MΩ, 0.5 ppm organics, Merck Millipore), dried with nitrogen flow and were then further cleaned by exposing to lowpressure air plasma ...
-
bioRxiv - Neuroscience 2023Quote: ... they were washed 3 times with 1X PBS for 5 minutes and incubated with DAPI (4’6-Diamidino-2-phenylindole; 1 µg/mL, MERCK) for 5 minutes ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were washed three times with 1 x PBS and mounted using an antifade mounting media (Sigma-Aldrich/Merck). The images were captured using a Zeiss AXIO Observer.Z1 Inverted Fluorescence microscope (Zeiss) ...
-
bioRxiv - Cell Biology 2024Quote: ... Blots were washed three times in TBS-T followed by incubation with horseradish peroxidase (HRP)-conjugated secondary antibodies (Merck). Following washing in TBS-T ...
-
bioRxiv - Neuroscience 2023Quote: ... cells were washed twice with PBS at room temperature (RT) and lysed in 1x (radio immunoprecipitation assay buffer (RIPA buffer, Merck Millipore, cat. no. 20-188) containing 0.1% SDS ...
-
bioRxiv - Microbiology 2022Quote: ... The PCR reaction was carried out using KOD Hot Start DNA polymerase (Merck) according to manufacturer’s instruction ...
-
bioRxiv - Synthetic Biology 2023Quote: ... PCR reactions were performed using a high-fidelity polymerase (KOD-Xtreme, Merck Millipore) according to manufacturer protocol ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... We carried out polymerase chain reactions (PCRs) with Hot Start polymerase (MERCK MILLIPORE) and performed colony PCRs with Taq polymerase (NEB) ...
-
bioRxiv - Microbiology 2020Quote: ... First strand cDNA preparations were diluted 1:10 with RNase-free water and subjected to RT-qPCR using SYBR Green JumpStart Taq ReadyMix (Merck Life Science UK Ltd., Gillingham, UK), with primers specified in Table S2 ...
-
bioRxiv - Cancer Biology 2021Quote: ... cells in IMDM+Glutamax medium were cryopreserved in liquid nitrogen until time of analysis complemented 1:1 with 80% FCS and 20% dimethyl sulfoxide (DMSO) (Merck).
-
bioRxiv - Developmental Biology 2021Quote: ... they were washed again in PBS (three times, each for 5 min) and mounted with antifade solution (Cat.no. S7114, Sigmsa-Aldrich, Merck, USA). Images were acquired using a Leica DM 2500 fluorescent microscope (Leica ...
-
bioRxiv - Immunology 2020Quote: ... After incubation cells were washed 3 times with PBS containing 0.5% BSA and subsequently fixed 1% (w/v) paraformaldehyde (PFA, Merck Millipore) in PBS ...
-
bioRxiv - Biochemistry 2020Quote: ... were incubated in standard assay buffer at 37°C and 2 µL aliquots were removed at appropriate time points and spotted on a silica gel 60 F454 plate (Merck). The separation was carried out in butanol:ethanol:milliQ water (5:3:2 ...
-
bioRxiv - Cell Biology 2019Quote: ... After the activation gels were washed three times with PBS and coated with either fibronectin (Merck-Millipore, catalog number 341631) (20 μg/ml ...
-
bioRxiv - Cell Biology 2021Quote: ... The blocks were then washed with DDW 2 times for 10 min and incubated in 1% (w/v) uranyl acetate (Merck)/70% (v/v ...
-
bioRxiv - Bioengineering 2022Quote: ... 100 nM of RNA polyhedra samples folded in TE/Mg2+/Na+ buffer were concentrated four times using 3 kDa MWCO Amicon Ultra centrifugal filters (Merck). 5 µl of the concentrated sample was applied on 300 mesh Cu grids coated with lacey carbon (Agar Scientific) ...
-
bioRxiv - Microbiology 2020Quote: ... Bacterial suspension of 106 CFU/ml would be obtained by a 100 times dilution before being spreaded on Mueller Hinton Agar (MHA - Merck) [12] ...
-
bioRxiv - Microbiology 2021Quote: ... were collected from the top of the gradient and the fraction material was cleaned by washing three times and resuspended in 0.02 µm-filtered FSW using 100 kDa Amicon-ultra filters (Merck Millipore). Vesicles were detected in fractions with densities of 1.05–1.07 g ml−1 (fractions 3-5 from the top).
-
bioRxiv - Neuroscience 2022Quote: ... The supernatant was discarded and the newly formed pellet was subsequently washed three times in total with 70% ethanol (Merck) and centrifuged at 20814 x g for 5mins ...
-
bioRxiv - Biochemistry 2021Quote: ... Reactions were stopped at each time point using 4 N formic acid and analyzed by PEI-Cellulose-F TLC (Merck) developed in 0.4 M potassium phosphate buffer (pH 3.4) ...