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Citations for Merck :
51 - 100 of 1119 citations for Zika Virus NS1 Proteins Uganda Suriname Strains Duo Pack since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: Gram staining of bacterial strains was performed using a Gram staining kit (Merck). Strain Ha5T cultured in LMG medium 404 broth for 24 hours was determined for cellular morphology using scanning electron microscope (FEI Quanta FEG 650) ...
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bioRxiv - Plant Biology 2022Quote: ... coli strains derived from BL21(DE3) and Rosetta 2 (Merck Millipore, Burlington, USA), BL21(DE3)-R3-pRARE2 and BL21(DE3)-R3-lambda-PPase ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The virus supernatants were directly overlaid on H1299 cells in the presence of polybrene (Merck, Germany) at a final concentration of 4 μg/ml ...
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bioRxiv - Microbiology 2022Quote: ... 10mL of virus was concentrated using centrifugation through Amicon® Ultra-15 Centrifugal Filter Units (Merck). Efficacy against steel was assessed at room temperature and 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Total RNA was isolated from the parasites (wild type RH strain) using TRIsoln (Merck). This was used to generate cDNA using a gene specific reverse primer and RevertAid Reverse Transcriptase (Thermo Scientific) ...
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bioRxiv - Biochemistry 2021Quote: Transformation of Escherichia coli strain BL21(DE3) Rosetta-2 pLysS (Novagen Merck, Darmstadt Germany) were made with a pET3d-His6-CrFBA3 plasmid ...
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bioRxiv - Microbiology 2022Quote: ... aureus strains were grown in Tryptic Soy Broth (TSB, ref. 22092 by Merck-Millipore), B ...
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bioRxiv - Microbiology 2023Quote: ... Bacterial strains were inoculated into 5 mL LB medium (Lysogeny Broth, Merck, Darmstadt, Germany) and grown for 5 hours ...
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bioRxiv - Microbiology 2023Quote: ... Bacterial strains were maintained on Lysogeny Broth (LB)-Miller or LB-Miller agar (Merck) and grown aerobically at 37°C overnight ...
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bioRxiv - Cancer Biology 2019Quote: ... The virus was harvested 48 hours post transfection and filtered through a 0.45 μm PES filter (Merck-Millipore ...
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bioRxiv - Cell Biology 2021Quote: ... Respiratory syncytial virus RSV-G was immunoblotted with commercially available goat polyclonal anti-RSV serum (#AB1128, Merck). Coxsackievirus VP1 was immunoblotted with commercially available mouse monoclonal anti-VP1 (3A8 ...
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ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ... virus was also concentrated using Amicon Ultra-15 Centrifugal Filter Units with 100 kDa cutoff (Merck Millipore). Mice were injected s.c ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 mL of virus supernatant is concentrated using Amicon Ultra-4 30K centrifugal filters (Merck, cat# UFC803024). For transduction of suspension cells ...
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bioRxiv - Microbiology 2023Quote: ... Strains were streaked out from stocks on tryptic soy agar (TSA; Merck KGaA, Darmstadt, Germany) and incubated overnight at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: The strains and samples were extracted using the RED Extract Plant PCR kit from Merck KGaA ...
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bioRxiv - Microbiology 2020Quote: ... and replaced with an appropriate amount of virus diluted in 0.5 mL of Dulbecco’s modified Eagle medium (Merck) with 1% FCS ...
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bioRxiv - Microbiology 2020Quote: ... virus inoculum was removed from each well and cells were overlaid with 300 μL of 0.6% carboxymethylcellulose (Merck) diluted in DMEM supplemented with 2% FBSi ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were transduced with 1 MOI of concentrated virus containing 8 ug/mL Polybrene (Merck, TR-1003-G). Two days later ...
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bioRxiv - Bioengineering 2023Quote: ... cells were stained with 1 µg (1:100 dilution) of a anti-Rabies Virus CVS-11 antibody (Merck) or anti-VZV gE mouse antibody (Abcam ...
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bioRxiv - Microbiology 2021Quote: To grow the bacterial strains Mueller Hinton broth (MHb) (Merck, Life Science UK Limited, Dorset, UK)) was used ...
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bioRxiv - Microbiology 2019Quote: For each experiment the strains were subcultured on Sabouraud dextrose agar medium (SDA; Merck, Darmstadt, Germany) for 48 h at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... The plasmid was used to transform Escherichia coli BL21 Rosetta2 (DE3) expression strain (Merck, Darmstadt, Germany), grown to exponential phase in 1 L lysogeny broth (LB ...
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bioRxiv - Immunology 2023Quote: ... Diphtheriae strain was incubated at 37°C in a shaking incubator in BHI broth (Merck, 53286) and was grown to an OD600 of 0.4 to 0.6 ...
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bioRxiv - Microbiology 2024Quote: ... Single colonies of the propagating strains were inoculated into LB medium (Lysogeny Broth, Merck, Darmstadt, Germany) and incubated at 37°C shaking at 170 rpm until reaching the exponential phase (approximately five hours) ...
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bioRxiv - Microbiology 2021Quote: ... One mL of virus stock and 9 mL of MEM-E supplemented with 1% pyruvate (Merck KGaA, Darmstadt, Germany) were added to a conical tube with 16 × 106 SK-6 cells and shaken for 30 minutes at 104 rpm and 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... 293T or 293GP virus producing cells were transfected using the GeneJuice reagent according to the manufacturer’s recommendations (Merck Millipore). Two days after transfection ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... One milliliter of the virus supernatants was directly overlaid on the cells in the presence of polybrene (Merck, Germany) at a final concentration of 4 μg/ml ...
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bioRxiv - Microbiology 2020Quote: ... 96-well plates with confluent cell layers were fixed and virus was inactivated by immersion in 100% methanol (MERCK) for 20 min ...
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bioRxiv - Genomics 2023Quote: ... The transduction was performed by adding the virus particle directly onto the cells supplemented with 6μg/mL polybrene (Merck). The cells were refreshed 24h after transduction and the antibiotics (puromycin/blasticidin ...
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bioRxiv - Microbiology 2023Quote: Purified proteins and native protein standard (Supelco-Merck) were loaded in 4-15% gradient mini protean precast native gel (Bio-Rad ...
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bioRxiv - Plant Biology 2020Quote: ... the strain was grown on solid King’s medium B (Pseudomonas agar F, Merck KGaA, 64271 Darmstadt, Germany) supplemented with 50 μg mL−1 rifampicin at 25°C in the dark for 3 days ...
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bioRxiv - Systems Biology 2020Quote: ... albicans strain SC5314 (ATCC MYA-2876) were cultivated on Tryptic Soy Agar (TSA) ReadyPlatesTM (Merck, Darmstadt, Germany) at 37°C overnight ...
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bioRxiv - Microbiology 2021Quote: 10His-tagged MA gene was expressed in Escherichia coli (E. coli) strain BL21 (DE3) (Merck, Darmstadt, Germany). The 10His-tagged MA protein was initially purified with nickel-NTA affinity column chromatography followed by TEV cleavage ...
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bioRxiv - Microbiology 2023Quote: ... The strains were first cultivated in separate pure cultures overnight on Tryptic soy agar (TSA; Merck, Germany) at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... coli RIL strains carrying the appropriate plasmid were grown in 1 l Luria-broth (LB) (Merck Millipore) supplemented with kanamycin (100 μg/ml ...
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bioRxiv - Microbiology 2021Quote: ... the pUL21 gene was amplified from virus stock by PCR with the oligonucleotide primers 5′-ATGGAGCTTAGCTACGCCAC-3′ and 5′-TTTATTGGGGTCTTTTACACAGACTGTC-3′ using KOD polymerase (Merck) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... For detection of particle incorporation virus supernatant was further concentrated by centrifugation at 4°C for 2h at 21000xg on 5% Optiprep (Merck), the supernatant was removed and pellet was resuspended and subjected to Western Blot analysis ...
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Telomerase deficiency in humans is associated with systemic age-related changes in energy metabolismbioRxiv - Cell Biology 2022Quote: Extraction of RNA from cell lines/strains was carried out using TRI Reagent (Merck Life Sciences, Dorset UK) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... One strain tube thawed rapidly at 37 °C and cultured in 10 ml Brain heart infusion (BHI - Merck) at 37 °C over 24 hours ...
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bioRxiv - Genomics 2021Quote: ... rosea strains was inoculated at edge of a 9-cm-diameter potato dextrose agar (PDA; Merck, Kenilworth, NJ) Petri plate covered with a durapore membrane filter (Merck ...
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bioRxiv - Microbiology 2022Quote: ... was applied onto the dried treatment (10mL of virus was concentrated using centrifugation through Amicon® Ultra-15 Centrifugal Filter Units (Merck). After 5 minutes at room temperature 150μl of DMEM (high glucose ...
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bioRxiv - Cell Biology 2023Quote: ... P1 virus was used to infect 50 ml of SF9 culture and P2 virus was harvested after 3-5 days through centrifugation and subsequent filtration through 0.45 μm PVDF membrane (Merck Millipore, SLHV033RS). P2 virus was either propagated further or stored at 4ºC in the dark ...
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bioRxiv - Neuroscience 2020Quote: ... individual COs were transferred to an Eppendorf tube containing CMM medium with 1:100 of virus containing medium and 5µg/ml Polybrene (Merck; TR-1003-6) and incubated over-night ...
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bioRxiv - Molecular Biology 2021Quote: hEnSC were seeded at a confluency of 105 cells/well in 6 well plates and 1.5 ml of concentrated virus added to each well in the presence of 8 μg/ml polybrene (Merck, TR-1003-G). Cells were then spinoculated at 500 x g for 1 hr at RT and incubated for 12 hrs ...
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bioRxiv - Molecular Biology 2021Quote: ... hTERT cells were transduced with 1.5 ml of concentrated pMY-SUZ12-FLAG-IRES-bls virus in the presence of 8 μg/ml polybrene (Merck, TR-1003-G). Cells were then spinoculated at 500 x g for 1 hr at RT and incubated for 12 hrs ...
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bioRxiv - Molecular Biology 2022Quote: ... The medium containing the virus was collected 48 hours post-transfection and filtered through a 0.45 µm polyethersulfone (PES) filter (Merck Millipore, Billerica, MA).
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bioRxiv - Plant Biology 2020Quote: ... The strains were routinely grown on solid King’s Medium B (KMB; Pseudomonas agar F, Merck KGaA, 64271 Darmstadt, Germany) supplemented with rifampicin 100 μg/mL at 24°C for 4 days ...
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bioRxiv - Developmental Biology 2023Quote: ... F1 generation of 8-week female hybrid mice (generated via C57BL6 female and CBA/W strain crosses) were intraperitoneally injected with 7.5IU of PMSG (pregnant mare serum gonadotrophin; Merck) and reinjected after 48 hours 7.5IU hCG (human chorionic gonadotrophic hormone ...
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bioRxiv - Plant Biology 2024Quote: A pTYB2-based vector for the expression of PSM polypeptides and a pCDF-based vector that contained heme oxygenase 1 and phytochromobilin synthase genes for the synthesis of PΦB (Muramoto et al., 1999; Kohchi et al., 2001) were transformed into the Escherichia coli Rosetta 2 strain (Merck). E ...
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bioRxiv - Microbiology 2024Quote: ... cerevisiae strains was investigated in SC -Ura medium supplemented with 1.5 ppm prothioconazole dissolved in 50 % DMSO (Merck, NJ) and measuring the OD600 in SpectraMax Gemini™ XPS/EM microplate reader (Molecular Devices ...