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Citations for Merck :
451 - 500 of 531 citations for Vitamin D Depleted Serum since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... All primary and secondary antibodies were diluted in a blocking solution consisting of PBS and 0.2% (v/v) bovine serum albumin (BSA, Merck). Cells were first washed with ice-cold PBS and incubated for 5 min in a blocking solution on ice ...
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bioRxiv - Neuroscience 2024Quote: Whole iPSC-brain organoids were permeabilized with 0.5% Triton X-100 in blocking solution with 2% Bovine Serum Albumin (BSA; Merck) in PBS for 3 hours at RT ...
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bioRxiv - Neuroscience 2024Quote: ... and then blocked for 1 h at RT with 0.2% Triton-X in PBS + 1% normal goat serum (NGS, Merck). Antibodies used and their concentration is found in Supplementary Table 4 ...
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bioRxiv - Neuroscience 2021Quote: ... coverslips containing fixed neurons were permeabilized and non-specific antigens blocked by incubating in D-PBS with 0.2% Triton-X (Merck, cat. X100-500ML) and 10% donkey serum (Merck ...
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bioRxiv - Neuroscience 2020Quote: ... subjects were given an overdose of sodium pentobarbital (Beuthanasia-D Special, 22 mg/100g body weight, Merck Animal Health, Madison, NJ, USA). Once at surgical plane ...
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bioRxiv - Neuroscience 2022Quote: ... Subjects were given an overdose of sodium pentobarbital (Beuthanasia-D Special, 22 mg/100g body weight, Merck Animal Health, Madison, NJ, USA). Once at surgical plane ...
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bioRxiv - Microbiology 2023Quote: ... 50 µl of each dilution were mixed with 10 µl of 0.4 mg ml-1 4-methyl-umbelliferyl-β-D- galactopyranoside (MUG) substrate (Merck, Darmstadt, Germany) that was prepared in dimethyl sulfoxide (DMSO ...
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bioRxiv - Synthetic Biology 2023Quote: ... VpALDH and SlAlaR were combined (each at 0.05 μM) with 1 U mL−1 D-amino acid oxidase (DAAO) from porcine kidney (Merck product no. A5222) and 2 U mL−1 catalase from bovine liver (Merck-MilliPoreSigma ...
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bioRxiv - Neuroscience 2023Quote: ... subjects were given an overdose of sodium pentobarbital (Beuthanasia-D, 22 mg/100 g body weight, Merck Animal Health, Madison, NJ, USA) and then transcardially perfused with approximately 250 mL of 25mM phosphate buffered saline (PBS ...
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bioRxiv - Plant Biology 2021Quote: ... A dilution series ranging from 1.25 μg ml−1 to 10 μg ml−1 bovine serum albumin (BSA, Merck KGaA) was used for calibration and processed together with the samples.
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bioRxiv - Microbiology 2021Quote: ... PBMCs (2 × 106 cells) were plated onto 48-well plates (NalgeNunc) in RPMI-1640 with 5% inactivated male human AB serum (Merck) for 3 hours ...
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bioRxiv - Microbiology 2021Quote: ... and human lung epithelial cell lines (Calu-3) were expanded in high glucose DMEM (Vero) or MEM (Calu-3) with 10% fetal bovine serum (FBS; Merck), with 100 U/mL penicillin and 100 μg/mL streptomycin (Pen/Strep ...
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bioRxiv - Developmental Biology 2021Quote: ... for 5 min at room temperature to loosen the zona pellucida and then treated with a hypotonic solution (1:1 mixture of 1.2% sodium citrate and 60% fetal bovine serum, FBS; Merck KGaA) for 10 min at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were permeabilized with 0.1 % Triton-X100 in PBS for 20 min at RT and samples were blocked with freshly prepared 3 % bovine serum albumin (BSA, Merck) in PBS for 1 h at RT ...
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bioRxiv - Neuroscience 2021Quote: ... was diluted (1:1000) in 500 μl 0.1% PBST with 0.2% normal donkey serum (S30-100ML, Merck Millipore, Hertfordshire, UK) and sections were protected from light and incubated at RT with agitation for 2 hours ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were incubated in low serum medium (0.5% FBS) containing 20 µM retinoic acid (RA) (Sigma-Aldrich; Merck KGaA) for 5 days ...
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bioRxiv - Neuroscience 2019Quote: ... A background block incubation in PBS with 1% TritonX with 10% Donkey serum was conducted before retinas were incubated in primary antibody solution (PBS with 1% Triton-X with 2.5% donkey serum with 1:250 dilution of rabbit anti-SWS cone opsin antibody, AB5407 Merck Millipore) overnight at 4°C ...
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bioRxiv - Physiology 2021Quote: ... with 0.2 % m/v Triton-X100 and 5% Normal Goat Serum (m/v) (S26-LITER, Merck Life Science UK LTD) (PBST.NGS ...
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bioRxiv - Biochemistry 2020Quote: ... the medium was replaced with a serum-free media supplemented with 2 nM brain-derived neurotrophic factor (BDNF) (Merck Millipore). After 2 days in the BDNF-containing media ...
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bioRxiv - Neuroscience 2021Quote: ... The cells were maintained in high glucose Dulbecco’s modified Eagle’s medium (HyClone, SH30243.02) supplemented with 10% EmbryoMax fetal bovine serum (Merck, ES-009-B) at 37°C with 5% CO2 and passaged every 3-4 days ...
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bioRxiv - Cancer Biology 2021Quote: ... blocked with 10% bovine serum albumin (BSA) 30 min at 37°C and incubated with primary anti-γH2A.X mouse antibodies (Merck Millipore) or rabbit anti-LC3B antibodies (Cell Signaling Technology ...
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bioRxiv - Microbiology 2020Quote: ... washed with PBS at room temperature before permeabilized for 30 min in PBS containing 0.1% Triton X-100 and 10% normal goat serum and incubated overnight at 4 °C in PBS containing 10% normal goat serum and primary antibodies: anti-Cytokeratin 14 (K14) antibody (SP53; Merck), anti-Neurofilament heavy polypeptide (NF200 ...
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bioRxiv - Cancer Biology 2020Quote: ... Human melanoma cell lines WM35 and WM793B were cultured in Dulbecco’s Modified Eagle’s Medium supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution (Merck, Darmstadt, Germany). For generation of conditioned melanoma media for immune cell inhibition experiments tryptophan (Merck ...
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bioRxiv - Cell Biology 2020Quote: ... in ice-cold serum-free RPMI media (R5886, Merck, with 100 U/μg/ml penicillin/streptomycin [P/S, P0781, Merck] and 2 mM L-glutamine [L-glut ...
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bioRxiv - Immunology 2020Quote: ... The sections were blocked in PBS containing 1% bovine serum albumin (BSA) for 1 h at RT and stained in blocking buffer containing primary antibody (anti-PP6C, Merck Millipore cat ...
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bioRxiv - Developmental Biology 2021Quote: ... They were incubated 1h at RT in a blocking buffer (20% BR + 20% goat serum) and then overnight with an anti-DIG-AP antibody (1:2000, Merck) in the blocking buffer ...
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bioRxiv - Neuroscience 2021Quote: ... MDMi cells were incubated with a primary antibody against Iba1 (1:1,000; 019-19744, FUJIFILM Wako Chemicals) in blocking buffer (2% bovine serum albumin, 0.1% TritonX100 (Merck, Darmstadt, Germany), and 5% normal donkey serum (017-000-121 ...
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bioRxiv - Neuroscience 2022Quote: ... slides were blocked for 30 min in 0.3 % Triton / 10 % normal goat serum / TBS followed by overnight primary antibody incubation (1:1000; rb TH; Merck Millipore) at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... the sections were treated with 10% methanol in PB for 20 min and then incubated for 1 h at RT in incubation buffer: 5% normal donkey serum (NDS: Merck) and 0.2% Triton X-100 (Merck ...
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bioRxiv - Physiology 2022Quote: ... They were then permeabilized with 0.02% Triton X-100 in PBS for 15 min and blocked with 5% bovine serum albumin (Sigma-Aldrich, Merck) in PBS for 3 h ...
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bioRxiv - Microbiology 2022Quote: ... ljungdahlii strain and its derivatives was carried out in 125 mL serum bottles (Sigma-Aldrich, Merck Life Science, Gillingham, UK) with a working volume of 30 mL ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were then placed in 10% horse serum/PB for 1 h before being incubated in primary goat anti-ChAT (1:500; #AB144P, Merck) for 2 d at 4°C to aid identification of CINs ...
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bioRxiv - Microbiology 2022Quote: ... cells were cultured in 25 cm2 flasks at 37°C and 5% CO2 using advanced Dulbeccos modified Eagle medium (DMEM; TFS) with the addition of 10% fetal bovine serum (Merck), 50 U/mL penicillin and 50 μg/mL streptomycin (TFS) ...
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bioRxiv - Pathology 2023Quote: ... Then sections were blocked for 45 min using a blocking buffer containing 5% goat or donkey serum (depending on the host species of the secondary antibody) (#G9023 and #D9663, Merck) and 0.3M glycine (#56-40-6 ...
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bioRxiv - Neuroscience 2023Quote: ... brain sections were washed three times for 15 min with 1X TBS and then incubated for 1.5 h in blocking solution: 2.5% Donkey Serum (Sigma, Merck, Germany, S30); 2.5% Goat Serum (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were starved (0% serum) for 24h and then treated for 96h with 5mM Metformin (Merck, cat. nr M0605000) or 2ng/ml TGF-β1 (Peprotech cat nr 10021) ...
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bioRxiv - Cancer Biology 2023Quote: ... T cell medium (human serum was replaced with FCS during thawing) and incubated for 15 minutes with 25U/mL benzonase (Merck). After washing ...
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bioRxiv - Cell Biology 2023Quote: ... Nuclei suspension was filtered, centrifuged and resuspended in RNAse-free PBS (AM9624, ThermoFischer Scientific) with 2% Bovine Serum Albumin (A2153, Merck) and 0.2 U/µL RNAse inhibitor (3335399001 ...
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bioRxiv - Biophysics 2020Quote: ... functionalized with either 100 μg/mL poly-D-lysine overnight for microglia cells or with 0.2 mg/mL fibronectin (FC010, Merck, 1:5 in PBS) for 2h at 37°C for fibroblasts ...
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bioRxiv - Developmental Biology 2021Quote: We mated Exosc10+/+ or Exosc10+/− females with stud Exosc10+/− male mice and recovered two-cell stage (gestational day 2; GD2) embryos by flushing approximately 0.5 ml of EmbryoMax Advanced KSOM medium (Merck-Sigma, MR-101-D) into the oviduct ...
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bioRxiv - Microbiology 2023Quote: ... These intestinal pieces were washed thoroughly with PBS and digested for 30 min at 37 °C with 0.5 mg/mL collagenase D (Merck/Sigma-Aldrich, cat#11088858001) in RPMI medium (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: ... Each imaging session was performed 15 minutes after intraperitoneal injecting D-Luciferin infusion (working concentration 15mg/ml, dose 10ml/Kg, MilliporeSigma, Merck KGaA, Darmstadt, Germany), considering the eLuciferase average flux (photons/second ...
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bioRxiv - Genetics 2023Quote: ... and neo-resistance MEFs were established from WT Crl:CD1 (ICR) or ICRneo [27] foetuses at 14 d post-coitus and inactivated using 10 μg/mL mitomycin C (#M0503; Merck KGaA, Darmstadt, Germany). MEFs were cultured in the primary medium [Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Neuroscience 2023Quote: ... We replenished the medium the next day and started selection against replicating non-neuronal cells on day two using neuronal medium containing 2 μM Cytosine β-D- arabinofuranoside (AraC) (Merck Life Sciences). We gradually diluted AraC out by replacing half of the medium with fresh neuronal medium every other day ...
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bioRxiv - Microbiology 2020Quote: ... IL-18 and TNFα were measured in serum samples using MILLIPLEX MAP Porcine Cytokine and Chemokine Magnetic Bead Panel - Immunology Multiplex Assay (Merck Millipore) following the manufacturer instructions ...
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bioRxiv - Physiology 2019Quote: ... following which they were treated with media containing 20% human serum (fed or fasted) and 1 µM puromycin (Merck Millipore Limited) for a further 4 h ...
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bioRxiv - Biochemistry 2020Quote: ... Next the medium was replaced with a serum-free media supplemented with 2 nM brain-derived neurotrophic factor (BDNF) (Merck Millipore). After 2 days in the BDNF-containing media ...
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bioRxiv - Biochemistry 2020Quote: ... cells were washed 3x in PBS followed by blocking in 16% (v/v) normal goat serum (Merck Milipore, Burlington, MA, USA) supplemented with 0.1% (v/v ...
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bioRxiv - Neuroscience 2021Quote: ... The enzymatic digestion was stopped by adding a plating medium (Dulbecco’s modified Eagle medium, D5796, Merck, containing 10% fetal bovine serum). Tissues were dissociated by pipetting ...
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bioRxiv - Cell Biology 2020Quote: ... with 4.5 g/l (high) glucose and L-glutamine supplemented with 10 % fetal bovine serum (volume fraction, Sigma Aldrich/Merck KGaA) without antibiotics at 37 °C and 5 % CO2 in a humidified atmosphere and regularly confirmed to be mycoplasma negative ...