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Citations for Merck :
401 - 450 of 3996 citations for Testosterone 100 Ug Ml In Methylene Chloride 3 4 13C2 99%; 17 18O 98% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Then Advanced DMEM/F12 medium was added supplemented with 100 µg/ml cycloheximide (Merck) to stop new protein synthesis and organoids incubated for the indicated release times at 37°C ...
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bioRxiv - Neuroscience 2019Quote: ... UK) and were perfused transcardially with at least 30 ml 4% paraformaldehyde (Merck, Dorset, UK) using a peristaltic pump ...
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bioRxiv - Biochemistry 2022Quote: ... 4 % glycerol) freshly supplemented with 5µg/ml Poly(dI-dC) (Merck Life Science cat. P4929) and 0.5 mM DTT ...
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bioRxiv - Immunology 2023Quote: ... permeabilized with 0.1% saponin in PBS and incubated with 4 μg/ml Hoechst 33342 (Merck) for 10 min at room temp ...
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bioRxiv - Synthetic Biology 2023Quote: ... 500 µl 0.2 µg/ml 4’,6’-diamidino-2-phenylindole (DAPI, Merck, catalog no. D9542) in PBS was added for 15 min at RT ...
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bioRxiv - Immunology 2024Quote: ... cells were treated for 4 h with 5 mg/ml Brefeldin A (Merck, Darmstadt, Germany) after 140 h incubation time ...
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bioRxiv - Microbiology 2023Quote: ... The strain was made ampicillin resistant by serial selections on Luria Broth (LB) agar (Carl Roth, Belgium) with increasing concentrations ampicillin (5 µL/mL to 100 µL/mL) (Merck, Belgium). Each time ...
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bioRxiv - Neuroscience 2023Quote: ... 10 µg of hGCase-1/17 or hGCase-1/23 or mouse IgG (Merck Millipore, #NI03) were coupled to 50 µl of Protein A magnetic Dynabeads in 200 µl Ab Binding and Washing Buffer for 30 minutes at RT on a rotating wheel ...
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bioRxiv - Cancer Biology 2022Quote: ... 200 μL of streptavidin sepharose beads (Streptavidin Sepharose High Performance, Merck/Millipore GHC-17-5113-01) were washed thrice in 50mM Tris-HCl pH 7.4 ...
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bioRxiv - Biochemistry 2020Quote: ... Chromatographic separation of AAs was achieved by applying 3 μl of dissolved sample on a SeQuant ZIC-HILIC column (3.5 μm particles, 100 × 2.1 mm) (Merck, Darmstadt, Germany), combined with a Javelin particle filter (Thermo Scientific ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Chromatographic separation was carried out on an Agilent 1200 series quaternary HPLC system using a Chromolith Performance RP18-e column (100×3 mm) from Merck operated a temperature of 45°C with 1 mM ammonium formate in water containing 0.1% FA as mobile phase A and 1 mM ammonium formate in 95/5 (vol/vol ...
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bioRxiv - Microbiology 2021Quote: ... + 3 μl benzonase (Novagen, Merck Millipore 70746-3), + 1 Roche complete protease inhibitor tablet) ...
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bioRxiv - Immunology 2021Quote: ... Histone neutralisation experiments were performed via intraperitoneal injection with dialysed and combined a-Histone 3 and a-Histone 4 antibodies (Merck Millipore) or control polyclonal rabbit IgG (BioXCell) ...
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P2RX7 inhibition reduces breast cancer induced osteolytic lesions - implications for bone metastasisbioRxiv - Cancer Biology 2022Quote: ... The cells were then stimulated with 100μM 2’(3’)-O-(4-Benzoylbenzoyl) adenosine-5’-triphosphate (BzATP; Merck Life Sciences, Gillingham, UK) to activate the P2RX7 ...
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bioRxiv - Biophysics 2019Quote: ... Fragments (2 mM in DMSO) were injected (2 μL) onto a Purospher STAR RP-18 end-capped column (3 μm, 30 × 4 mm, Merck KGaA). Chromatographic separation was carried out over a 4-min gradient elution (90:10 to 10:90 water:methanol ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were centrifuged (1500 g for 5 min at 4°C) and resuspended in 3 x PCV buffer A + 0.1% NP40 (Merck Life Science). After 10 further min on ice ...
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bioRxiv - Systems Biology 2020Quote: ... which includes 5% of PBS buffer and 5% acetonitrile in 0.15 M sodium chloride solution (Merck, Darmstadt, Germany). The pH of equilibration buffer was 7.2 ...
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bioRxiv - Cell Biology 2022Quote: ... and then transferred into activation medium (M16 medium without CaCl2 containing 100mM Strontium chloride, Sigma-Aldrich Merck 204463) for 1 hour ...
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bioRxiv - Cell Biology 2019Quote: ... kidneys were removed and washed in Hanks’ Balanced salt solution (HBSS containing calcium chloride and magnesium sulphate, Merck). Kidneys were then cut into small pieces and further dissociated by incubation for 30 min at 37°C in HBSS with 1 mg/ml collagenase (type 2 ...
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bioRxiv - Immunology 2021Quote: ... were coated overnight at 4 °C with 100 µL of lysate diluted at 1:40 in carbonate buffer (Merck). After a blocking step with PBS with 4 % milk for 1 h ...
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bioRxiv - Microbiology 2021Quote: Cells grown on glass coverslips were fixed in 4% PFA for 30 min at RT and permeabilized with 0.2% Triton X-100 (Sigma/Merck) in PBS for 10 min at RT ...
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bioRxiv - Immunology 2022Quote: ... The sections were incubated overnight at 4°C with the following primary antibodies: KRT8 (Merck Millipore, MABT329, 1:100), p-ERK (Cell Signaling Technology ...
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bioRxiv - Bioengineering 2023Quote: ... for 20 min at room temperature (RT) and permeabilised overnight at 4 °C in 0.5% Triton X-100 (Merck) solution in PBS ...
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bioRxiv - Developmental Biology 2020Quote: ... all other conditions: N=3) in the presene of 4µg/ml polybrene (cat. TR-1003-G, Merck) (Supplementary Figure 2) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... excess media was removed from wells and mf were incubated with 0.5 mg/ml MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (Merck) in PBS at 37°C for 90 min ...
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bioRxiv - Immunology 2021Quote: ... Calu-3 cells were transduced by addition of lentiviral supernatants containing 8 μg/ml polybrene (Merck Darmstadt). 48 hours after transduction ...
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bioRxiv - Biochemistry 2022Quote: ... # T7408) using an ultrafiltration cartridge (Amicon Ultra 0.5 mL 3 K; Merck, Readington, NJ, USA; Cat. # UFC500324). The total protein levels in the samples were assayed using the Pierce™ BCA Protein Assay kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... 5% of input material was taken for further analysis and 150 μL of pre-washed Streptavidin Mag Sepharose® (GE28-9857-99; Merck) were added to the remaining material ...
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bioRxiv - Cell Biology 2020Quote: ... as described above. The pretreatments with translational inhibitors puromycin (f. c. 20 µg/ml) and cycloheximide (f. c. 100 µg/ml) (Merck, Darmstadt, Germany) were performed after harvesting of the cells in the import buffer for 30 min at 37°C.
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bioRxiv - Plant Biology 2021Quote: ... USA) was conditioned with 1 ml of 100% methanol and 1 ml of deionized water (Milli-Q, Merck Millipore, Burlington, MA, USA). After the conditioning steps ...
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bioRxiv - Genetics 2020Quote: ... Cells were left untreated or treated with IFN-α2b (INTRON A, Merck, 100 IU/ml) or IFN-λ3 (R&D Systems ...
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bioRxiv - Microbiology 2023Quote: ... and NTK seeded on surfaces coated with 100 μg/ml type IV human collagen (Merck) in defined keratinocyte serum-free medium (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... 100 μg of protein were loaded into FASP filters (Amicon Ultra 30K, 0.5 ml, Merck) and tryptically digested over night following the FASP (Filter-Aided Sample Preparation ...
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bioRxiv - Bioengineering 2024Quote: ... Excess staples were removed by 100 kDa Amicon Ultra 0.5 ml centrifugal filter units (Merck). In brief ...
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bioRxiv - Bioengineering 2021Quote: ... CD14+-cells were detached in a similar manner: 4 mg/mL lidocaine hydrochloride (L5647; Merck KGaA) were solved in Versene Solution freshly for each detachment ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 ml of each sample were concentrated with Amicon ultra Centrifugal filters (Cat no. UFC510024, Merck). Samples were centrifuged (14 000 × g ...
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bioRxiv - Neuroscience 2023Quote: ... with a stock solution of gramicidin A (4 mg/ml - dissolved in dimethyl sulfoxide, DMSO, Merck) to achieve a final concentration of 80 μg/mL gramicidin19 ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions containing full-size protein were concentrated using Amicon®Ultra 4 mL centrifugal filters (Merck). After the addition of 10% glycerol ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions containing full-size protein were concentrated using Amicon®Ultra 4 mL centrifugal filters (Merck). After the addition of 10% glycerol ...
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bioRxiv - Biochemistry 2020Quote: ... proteins were transferred to 100 mM bicarbonate buffer (pH 8.2) by using Amicon® Ultra Centrifugal Filters (3 kDa MWCO, Merck, USA) and mixed with 1 ...
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bioRxiv - Cell Biology 2022Quote: ... The selected elution fractions (1-3 of 500 μL each) were pooled and subsequently concentrated using 100-kDa Amicon centrifugal filter units (Merck Millipore). The concentrated samples were subjected to several washing steps with PBS to obtain a highly pure EV population ...
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bioRxiv - Bioengineering 2023Quote: ... samples were washed in PBS for 3 times and Alexa Fluor 555 conjugated secondary goat anti-mouse IgG antibody (1:100; Merck, Germany) was added for immunostaining in the dark for 2 hours at RT ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were subsequently washed 3 times for 10 minutes with PB containing 0.2 % Triton X-100 (Merck, Darmstadt, Germany, 108603; PBT). Next ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were subsequently washed 3 times for 10 minutes with PB containing 0.2 % Triton X-100 (Merck, Darmstadt, Germany, 108603; PBT). Next ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with a cocktail of 1:100 phosphatase inhibitors cocktail 2 and 3 (Sigma/Merck, P5726-1ML and P0044-1ML, respectively) and 1:100 protease inhibitor cocktail (Sigma/Merck ...
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bioRxiv - Molecular Biology 2020Quote: ... Chromatin-IP was carried using the ChIP assay kit (EZ-ChIP TM; Cat#17-295) from Merck Millipore ...
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bioRxiv - Cancer Biology 2022Quote: ChIP experiments were performed using EZ-Magna ChIPTM A/G Chromatin Immunoprecipitation Kit (Merck Millipore, 17-10086) according to the manufacturer’s procedures ...
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bioRxiv - Physiology 2023Quote: ... EdU was detected directly by click chemistry according to the manufacturer’s instructions (17-10525, Merck, Darmstadt, Germany). Fluorescence was imaged by using an Olympus IX81 confocal microscope (Olympus ...
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bioRxiv - Developmental Biology 2022Quote: ... 32.8 mM 20:4 and 60 mM 22:6) and pipetted dropwise into N2 media containing 3 mM fatty acid-free BSA (Merck, Cat. #A8806) at 37°C with constant stirring to obtain final concentrations of 3 mM 18:0 ...
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bioRxiv - Microbiology 2022Quote: ... Slides were mounted in CitiFluor™ CFM3 mounting medium (proSciTech, Australia) containing 3 μg/uL 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI; Merck, Germany) to stain nucleic acids ...