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Citations for Merck :
1 - 50 of 993 citations for TIM 3 Human HEK 293 Fc His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... HEK 293 cells were seeded in culture plates (Corning, Merck, Darmstadt, Germany) and incubated in DMEM (PAN Biotech ...
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bioRxiv - Neuroscience 2021Quote: HEK 293 cells were seeded in cell culture plates (Corning, Merck, Darmstadt, Germany) in the day before at the appropriate density ...
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bioRxiv - Immunology 2020Quote: ... FITC conjugated goat anti-human IgG Fc was from Merck, Dorset ...
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bioRxiv - Genomics 2020Quote: ... was incubated with/without 0.4μg human 6X-His-CDK9/Cyclin T1 (Merck, 140685) and/or 0.4μg human GST-tagged PP2A-α (Sigma ...
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bioRxiv - Bioengineering 2021Quote: Three sender area squares (3 × 3 mm) were put into a 24-well containing 300 μL of anti-His antibody (Merck, Germany, NOVG70796-3) (10 μg mL−1 in DPBS ...
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bioRxiv - Molecular Biology 2021Quote: ... and goat anti-Human IgG (Fc specific)-Cy3 (1:1000 dilution in binding buffer; Merck). Finally ...
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bioRxiv - Biochemistry 2023Quote: ... HEK-293T cells were transfected at ∼40 % confluency using a 3:1 polyethylenimine (branched, average Mw ∼25,000 Da; Merck) to DNA ratio (30:10 μg ...
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bioRxiv - Molecular Biology 2022Quote: ... or anti-His (Merck) for His-G5845–1508) ...
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bioRxiv - Cancer Biology 2023Quote: ... T cell medium (human serum was replaced with FCS during thawing) and incubated for 15 minutes with 25U/mL benzonase (Merck). After washing ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were purified with His-Bind Resin and His-Bind Buffers (Merck Millipore) according to manufacturer’s protocol and stored at a concentration of 2mg/ml in elution buffer (Merck Millipore ...
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bioRxiv - Immunology 2023Quote: ... using the 293-free transfection reagent (Merck, Darmstadt, Germany) according to the manufactures’ protocols ...
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bioRxiv - Immunology 2019Quote: ... Fc IgG (AG714, Merck Millipore) at 2 μg/ml in 150 μl of PBS for one hour ...
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bioRxiv - Biophysics 2019Quote: ... supplemented with 10 % FCS (Merck), 100 µg ml−1 penicillin (Life technologies) ...
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bioRxiv - Biophysics 2019Quote: ... supplemented with 10% FCS (Merck), 100 µg ml−1 penicillin (Life technologies) ...
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bioRxiv - Biophysics 2020Quote: ... supplemented with 10% FCS (MERCK), 100 μg ml−1 penicillin (Life technologies) ...
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bioRxiv - Biochemistry 2021Quote: ... supplemented with 10% FCS (Merck) and 2 mM L-glutamine (Thermo Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... + 10% FCS (Merck Millipore S0615) + 1% P/S (Thermo Fisher #15140122) ...
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bioRxiv - Immunology 2024Quote: ... supplemented with 10% FCS (Merck), 2mM L-Glutamine (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... His-tagged proteins were immobilized on Ni-NTA His-Bind® Superflow™ Resin (Merck Millipore, 70691), washed and eluted in SB buffer (30 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Neuroscience 2021Quote: 293 cells were seeded in culture plates (Corning, Merck, Darmstadt, Germany) and incubated overnight at 37°C with 5% CO2 in DMEM (PAN Biotech ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% HI-FBS (Merck/Biochrom), 2 mM L-glutamine (Merck/Biochrom) ...
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bioRxiv - Systems Biology 2023Quote: ... Primary his tag antibody (05-949, Merck), stored in a skim milk solution ...
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bioRxiv - Molecular Biology 2024Quote: ... or pET30c (HIS) (Merck/Novagen, Darmstadt, Germany) using EcoRI and NotI sites ...
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bioRxiv - Cell Biology 2020Quote: ... containing 10% FCS (Merck KGA, Germany), 4 mM glutamine (Merck KGA ...
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bioRxiv - Biochemistry 2023Quote: ... Antibodies usedwere: mouse monoclonal (BIII-136) anti human Band 3 (B9277, Merck Life Science S.r.l., Milan, Italy). Mouse monoclonal (H68.4 ...
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bioRxiv - Immunology 2020Quote: ... and 10% Fetal calf serum (FCS) (Merck). Both cell lines were cultivated in T75 culture flasks ...
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bioRxiv - Biophysics 2021Quote: ... 6×His-tagged recombinant MSP1E3D1 (Sigma-Aldrich, Merck, USA) and FLAG-tagged recombinant mCardinal (kindly provided by Jakub Czapiński ...
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bioRxiv - Biochemistry 2024Quote: ... we utilized the His-Bind purification kit (Merck, USA) followed by manufacturer’s protocol ...
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bioRxiv - Biophysics 2019Quote: ... and 10 ml RPMI containing 0.75 % FCS (Merck) in a common 50-ml Eppendorf tube and heated up to 68°C using a common water bath ...
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bioRxiv - Biochemistry 2021Quote: ... Fluorinated oil (FC- 40, Merck, Kenilworth, NJ, USA) was used to remove the excess amounts of the reaction solution and to seal the reactor (Supplementary Fig ...
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bioRxiv - Bioengineering 2023Quote: ... FCS was mixed with 50% PEG-10,000 (Merck) at a 5:1 ratio and rotated at 4 °C for 3 h ...
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bioRxiv - Microbiology 2020Quote: ... HEK 293T cells grown on 15-cm dishes were transfected using polyethylenimine (PEI) (Merck), a transfection reagent for standard triple transfection protocol ...
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bioRxiv - Bioengineering 2021Quote: The rat INS-1 832/3 cell line (insulinoma cell line stably transfected with human insulin; hereinafter INS-1) was obtained from Merck. A HUVEC (human umbilical vein endothelial cell ...
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bioRxiv - Genetics 2021Quote: ... At day 10 cells were passaged at a 2:3 ratio into 12 well cell culture plates coated with 15 µg/ml human plasma fibronectin (Merck) in Dulbecco’s phosphate-buffered saline (DPBS ...
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bioRxiv - Genomics 2020Quote: ... supplemented with 15% fetal calf serum (FCS) (Merck Millipore), 0.1mM b-mercaptoethanol (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2022Quote: ... was supplemented with 10% fetal calf serum (FCS) (Merck), 1x non-essential amino acids (NEAA ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... supplemented with 10% foetal calf serum (FCS, Merck, Germany). Once confluent ...
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bioRxiv - Bioengineering 2022Quote: ... supplemented with 10% Fetal Calf Serum (FCS, #S0615, Merck), 4 mM L-glutamine (#25030-024 ...
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bioRxiv - Biophysics 2021Quote: ... anti-6×His and anti-FLAG M2 (Sigma-Aldrich, Merck, USA). The anti-mouse IgG ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was added to a His Trap HP column (Merck) previously equilibrated with buffer A ...
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bioRxiv - Cell Biology 2021Quote: ... previously coupled to 1 ml HI-TRAP NHS columns (GE/Merck) packed with Sepharose pre-activated for covalent coupling of ligands containing primary amino groups ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS; Merck). Immortalized human C20 microglia were kindly provided by David Alvarez-Carbonell (21) ...
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bioRxiv - Plant Biology 2022Quote: ... loaded onto His-Bind® Resin columns (EMD Millipore; Merck KGaA), and the recombinant ZmEREB57 protein was eluted with elution buffer (0.5 M NaCl ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... rat slices were exposed to 3 μM dihydroethidium (DHE) while in humans’ sections it was added 4 μM DHE (Merck, Darmstadt, Germany) for 30 minutes at 37ºC 51 ...
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bioRxiv - Cell Biology 2023Quote: ... and anti-mouse IgG (Fc) TRITC (Merck, #SAB3701020, 1:500).
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bioRxiv - Cell Biology 2023Quote: ... HeLaM and HEK-293T cells were grown in Dulbeccos modified Eagles medium (DMEM) (Merck, D6429-500ML) supplemented with 10% Foetal bovine serum (FBS ...
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bioRxiv - Microbiology 2021Quote: ... and the supernatant was incubated with His-binding resin (Merck 69670-5) for 10-30 min at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... the supernatant was loaded onto a His-bind resin column (Merck Millipore) (1 ml ...
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bioRxiv - Plant Biology 2023Quote: ... and purified with Ni-NTA His•Bind® Resin agarose (Merck, 70666). The binding reaction was performed in 20 µL of binding buffer [10 mM Tris (pH 8.0) ...
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bioRxiv - Biochemistry 2023Quote: ... His tagged proteins were labeled using Ni-NTA-ATTO 488 (Merck Group) at a ratio of ATTO/protein = 1/500 in MN buffer (50 mM MES pH 6 ...