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Citations for Merck :
301 - 350 of 1031 citations for TGF beta 4 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... with PBS pH 7.4 for 10 min at 4400 x g and 4°C using an Amicon Ultra-4 concentrator with 3 kDa cutoff (Merck Millipore). The degree of labelling (DOL = 1.96 ...
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bioRxiv - Neuroscience 2022Quote: ... Unencapsulated molecules were removed by washing at least 4 times the LPs suspensions with PBS pH 7.4 for 20 min at 4°C and 5000 x g using an Amicon Ultra-4 concentrator with 100 kDa cutoff (Merck Millipore). The degree of encapsulation was calculated by subtracting from the total mass of the molecule to be encapsulated that present in the flow-through and was found to be higher than 75% in all LP preparations.
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bioRxiv - Cell Biology 2023Quote: ... Fixed cells were permeabilized with 0.01% saponin (10 minutes, 4°C) and labeled with Phalloidin-TRITC (Merck, 30 minutes, 4°C). After washing ...
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bioRxiv - Cancer Biology 2024Quote: ... or expressing the sequence of 4-1BBL (Vector Builder, pLV[Exp]-Hygro-EF1A>4-1BBL) using X-tremeGENE™ HP DNA Transfection Reagent (Merck).
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bioRxiv - Microbiology 2019Quote: ... Solid phase synthesis took place on a CF peptide synthesizer (Intavis) using a Fmoc-PEG-Biotin NovaTag ™ resin (100 µmol; Merck), 2-Azidoacetic acid (Fluorochem ...
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bioRxiv - Immunology 2020Quote: ... Peptides were separated from the HLA molecule remnants by ultrafiltration employing 3 kDa and 10 kDa Amicon filter units (Merck Millipore) for HLA-I and HLA-II ...
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bioRxiv - Microbiology 2020Quote: ... Samples were then resuspended in 10 μl of 0.1% trifluoroacetic acid and tryptic peptides were desalted using a Zip-Tip® column (Merck Millipore) pipette tip according to the manufacturer’s protocol and dried in a vacuum concentrator before analysis using liquid chromatography-tandem mass spectrometry (LC-MS/MS).
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bioRxiv - Biochemistry 2023Quote: All peptides used in this study probes were designed as 11-mers bearing biotinylated PEG2 linker in the N terminus and an amide group in the C terminus and were synthesized using Fmoc-solid-phase peptide chemistry with Novasyn TGR resin (Merck Millipore) as described in the Novabiochem Innovations 01/11 ...
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bioRxiv - Systems Biology 2024Quote: ... the acidified peptide solution was cleaned-up on self-packed StageTips (70) accommodating four discs of Empore C18 material (Merck Supelco). The material was prepared by consecutive application of pure ACN (Biosolve Chimie) ...
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bioRxiv - Cancer Biology 2024Quote: ... a cassette coding for tdTomato with an N-terminal nuclear localization sequence and lack of a stop codon and a puromycin cassette with an N-terminal self-cleaving T2A peptide were amplified by KOD polymerase (Merck, 71085) and subjected to Gibson assembly.
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were blocked using 2% normal goat serum and stained using the following primary antibodies in blocking solution overnight: anti-Ng2 (1:100, rabbit pAb, AB5320 Merck Millipore, Burlington, MA), anti-beta III tubulin (1:100 ...
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bioRxiv - Cell Biology 2021Quote: The vessel lumen was washed 3 times with PBS ++ (PBS with 1mM CaCl2, 0.5mM MgCl2) and fixed with 4% paraformaldehyde (PFA, Merck, #30525-89-4) at 37°C for 15 min ...
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bioRxiv - Plant Biology 2022Quote: ... The lysate was cleared by two centrifugations (4000 rpm, 10 min, 4 °C and 20000g, 10 min, 4°C) and filtered (Miracloth, Merck 475855-1R). The lysate (input ...
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bioRxiv - Cell Biology 2023Quote: ... The cell lysates were centrifuged at 16,000 x g for 10 minutes at 4°C and the supernatants were then incubated overnight at 4°C with anti-Flag M2 affinity gel beads (Merck, Cat.N. A2220). M2 beads were then pelleted and washed three times with lysis buffer containing protease inhibitors ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: OCT-embedded penis sections from humans and rats of 6-8 μm were cut in a cryostat and incubated with or without 4-Hydroxi-TEMPO (4-TEMPOL; Merck, Darmstadt, Germany) 10mM for 30 minutes ...
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bioRxiv - Microbiology 2023Quote: ... proteins were ultracentrifuged at 13000 rpm for 10 min at 4°C and concentrated to 1.5 ml using centrifugal filters (Merck, Amicon Ultra-4). They were then exchanged into buffer #5 overnight at 4°C and aliquoted and stored at -80°C ...
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bioRxiv - Cell Biology 2020Quote: ... Coverslips were mounted in MOWIOL 4-88 (Merck-Millipore). All steps were conducted at room temperature and in the dark post-secondary antibody addition ...
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bioRxiv - Neuroscience 2021Quote: ... coverslipped with 20 μl Mowiol® 4-88 (Merck Life Science UK Ltd ...
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bioRxiv - Biochemistry 2020Quote: ... and 4 μg of unconjugated mouse IgG2a (Merck #M5409) per 100 μL for detection of CD235a ...
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bioRxiv - Plant Biology 2020Quote: ... followed by 4% (v/v) sodium hypochlorite (MERCK 1.93607.1021) containing 0.02% Triton X-100 for 3-min and rinsed three times with sterile distilled water ...
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bioRxiv - Physiology 2021Quote: ... 0.004% (w/v) 4-hydroxybenzoate sodium salt (Merck, Germany)).
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bioRxiv - Cell Biology 2021Quote: ... and with 4 μM CHIR99021 (Merck Millipore Sigma, USA) for one day ...
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bioRxiv - Biophysics 2021Quote: ... and 4 mM of CaCl2 (Merck Life Science, Norway) (pH 7.8 adjusted with NaOH).
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bioRxiv - Cell Biology 2022Quote: ... cultures were fixed in 4% paraformaldehyde (Sigma-Aldrich, Merck) for 30 min and washed in distilled water ...
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bioRxiv - Cancer Biology 2022Quote: ... 4′,6-diamidino-2-phenylindole (DAPI; Merck Life Science) 0.1 µg/mL was added to the medium to exclude dead cells.
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bioRxiv - Cancer Biology 2021Quote: ... 10 and 3 kDa (Amicon Ultra-4, Merck Millipore) to fractionate the proteins according to their size ...
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bioRxiv - Physiology 2021Quote: ... 0.004% (w/v) 4-hydroxybenzoate sodium salt (Merck, Germany)).
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bioRxiv - Developmental Biology 2022Quote: ... 4 mg/ml bovine serum albumin (BSA, Merck, A3311), penicillin-streptomycin (75 U/ml-60 μg/ml ...
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bioRxiv - Molecular Biology 2022Quote: ... filters were fixed in 4% paraformaldehyde (PFA; Merck, P6148) and permeabilized with 0.1% Triton X-100 (Merck ...
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bioRxiv - Neuroscience 2022Quote: ... and sugammadex sodium (4 to 8mg/kg, i.v.; Merck Sharp & Dohme Corp. ...
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bioRxiv - Neuroscience 2022Quote: ... Coverslips were mounted with Mowiol 4-88 (Merck Chemicals). For detection of Jacob-CREB and Jacob-LMO4 co-localization in STED imaging ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then fixed with 4% paraformaldehyde (PFA; Merck) in PBS for 15 min ...
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bioRxiv - Molecular Biology 2023Quote: ... using 4 µM calcium ionophore A23187 (cat. C7522, Merck). The GSK484 concentrations were 1 µM ...
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bioRxiv - Microbiology 2023Quote: ... Tyrosol [2-(4-hydroxyphenyl) ethanol] (Merck Ltd., Budapest, Hungary) was prepared as a 0.1 M stock solution in sterile physiological saline.
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Identification of resistance mechanisms to small-molecule inhibition of TEAD-regulated transcriptionbioRxiv - Cancer Biology 2023Quote: ... cells were fixed with 4% paraformaldehyde (Merck life sciences) and stained with 1µg/ml DAPI (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... 4’,6-diamidine-2’-phenylindole dihydrochloride (DAPI) (Merck #10236276001) at a final concentration of 1 µg/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were fixed with 4% formaldehyde (Merck, Sigma Aldrich) in PBS (phosphate buffered saline ...
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bioRxiv - Neuroscience 2020Quote: ... glial glutamate transporter GLT1 (guinea pig, polyclonal, synthetic peptide from the C-terminus of rat GLT1, Merck, AB1783, AB_90949, dilution 1:1,000), GFP (chicken ...
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bioRxiv - Plant Biology 2021Quote: ... 1000 μl tips were fitted with 2 plugs of C18 octadecyl 47mm Disks 2215 (Empore™) material and 10 μg of LiChroprep® RP-18 peptides (Merck). Tips were sequentially equilibrated with 100 % methanol ...
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bioRxiv - Plant Biology 2021Quote: ... Extracted peptides were dried in a speed-vac and resuspended in (v/v) 2% acetonitrile/0.2% trifluoroacetic acid (Merck, catalog no. 302031). A total of four biological replicates for each sample type was submitted.
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bioRxiv - Plant Biology 2023Quote: ... Pulldowns were done as described17 with the following modifications: The biotinylated Aux/IAA7 DII peptide (AKAQVVGWPPVRNYRKN, N-terminal biotin) was bound to streptavidin-agarose beads (Merck, #16-126). 250 μl of total protein extract was combined with 45 μl of DII-beads and either 0 μM or 10 μM cvxIAA or 10 μM IAA ...
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bioRxiv - Molecular Biology 2021Quote: ... Chromatin corresponding to 13 μg of DNA was diluted 10-fold in RIPA buffer without SDS complemented with protease inhibitors and incubated overnight at 4°C with 4 μg of H3K9me3 or H3K27me3 antibodies (Frapporti et al., 2019) or with 4 μL of H3K4me3 monoclonal antibody (Merck Millipore #04-745). As input control ...
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bioRxiv - Genomics 2019Quote: Cells were seeded at a density of 4 × 104 cells per well in laminin coated 4 well chamber slides (Millicell® EZ slide, Merck Millipore, PEZGS0816). Cells were washed in cold PBS and fixed using 4% paraformaldehyde (PFA) ...
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bioRxiv - Plant Biology 2020Quote: ... the ORF of CLEL6 lacking the predicted signal peptide was amplified by PCR from a previous construct and cloned into the NcoI restriction site of pETDuet1 (Novagen/Merck KGaA, Darmstadt, Germany). Correct orientation and translational fusion with the C–terminal His-tag was verified by sequencing ...
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bioRxiv - Plant Biology 2020Quote: ... ORFs of PSK1 and RGF1 lacking the predicted signal peptides were amplified by PCR from cDNA and cloned into the NcoI restriction site of pETDuet1 (Novagen/Merck KGaA, Darmstadt, Germany). C-terminal His tags were added by including 6 His codons in the reverse PCR primers ...
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bioRxiv - Microbiology 2021Quote: ... A total of 4 mL of the wash was extracted and placed in a Amicon® Ultra-4 Ultracel®-50k (Merck Millipore Ltd.) filter tube and centrifuged for 7 minutes at 7000 rpm ...
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bioRxiv - Plant Biology 2024Quote: ... 2-(1H-Indol-3-yl)-4-oxo-4-phenyl-butyric acid (PEO-IAA; OlChemIm, Olomouc, Czech Republic) and indole-3-carbinol (I3C; Sigma-Aldrich, Merck KGaA, Darmstadt, Germany).
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Controlled Fluorescent Labelling of Metal Oxide Nanoparticles for Artefact-free Live Cell MicroscopybioRxiv - Biophysics 2021Quote: ... centrifugal filter device Amicon Ultra 4 mL 100K (Merck Millipore), HCl (Merck) ...
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bioRxiv - Cell Biology 2020Quote: ... in 20 µl reactions containing 4 mM ATP (#1191, Merck), 150 mM NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... The sections were stained with 4% uranyl acetate (Merck, Germany) and 2% methylcellulose at a ratio of 1:9 (on ice) ...