Labshake search
Citations for Merck :
151 - 200 of 264 citations for T 25 Flasks since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2024Quote: ... Membranes were washed 3 times with PBS-T for 5 min and incubated with mouse-HRP (A4416; Merck) and rabbit-HRP (GENA9640V ...
-
bioRxiv - Cell Biology 2024Quote: ... cover slips were incubated with the primary antibody (SV40 T antigen mouse-anti-human, Merck-Millipore, 1:50) overnight at 4 °C in a humidifier chamber ...
-
bioRxiv - Immunology 2022Quote: ... T cells were isolated by dissociating tumor tissue in the presence of collagenase D (1 mg/ml) (Roche-Merck) for 10 min at 37°C ...
-
bioRxiv - Molecular Biology 2022Quote: ... CD3+ T cells were cultured in TexMACS Medium (Miltenyi Biotech) supplemented with 5% heat-inactivated human AB serum (Merck), 100 units/mL penicillin (Merck ...
-
bioRxiv - Developmental Biology 2023Quote: ... the HCR protocol involved rehydrating the gastruloids with a descending series of methanol concentrations and finally PBS-T (0.1% Tween, 655204, Merck). Once hydrated ...
-
bioRxiv - Microbiology 2024Quote: ... the membranes were incubated o/n at 4°C in 10 ml washing buffer (1% [w/v] milk powder in TBS-T) containing a 1:10,000 dilution of anti-pan-ADP-ribose binding reagent MABE1016 (Merck) at 4°C48 ...
-
bioRxiv - Cell Biology 2024Quote: ... Blots were washed three times in TBS-T followed by incubation with horseradish peroxidase (HRP)-conjugated secondary antibodies (Merck). Following washing in TBS-T ...
-
bioRxiv - Cell Biology 2022Quote: ... at a density of 25×103 cells/cm2 and cultured in EndoGro medium (Merck Millipore, Darmstadt, Germany). TEER was measured daily and experiments were performed when it reaches over 30 Ωcm2 (5-7 days after seeding) ...
-
bioRxiv - Microbiology 2021Quote: ... GGG-azide labelled proteins were concentrated to 25 μM on a 30 kDa Amicon Tube (Merck Millipore) in Tris/NaCl buffer and next labelled with 100 μM DBCO-Cy5 (Sigma Aldrich ...
-
bioRxiv - Microbiology 2020Quote: ... Lentivirus stocks were concentrated 25-fold using centrifugal protein concentrators (Amicon Ultra, 10-kDa cutoff; Merck, Poland) and stored at - 80°C.
-
bioRxiv - Molecular Biology 2021Quote: ... The lysate was sonicated in chilled water bath and treated with 25 units of Benzonase (Merck Millipore) for 1 h at 4°C ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were trypsinized and seeded onto coverslips that were coated with 25 µg/ml laminin (Merck, #11243217001) in laminin coating buffer (50mM TRIS ...
-
bioRxiv - Developmental Biology 2022Quote: ... Post-mating, the female rabbit was injected intravenously luteinising hormone (dose 25 i.u., Luveris (75 IE, Merck)) ...
-
bioRxiv - Cell Biology 2023Quote: ... 25 µl iRBCs were incubated in 200 µl cRPMI supplemented with 100 nM Rapamycin (Sigma-Aldrich, Merck) or 1% DMSO (Sigma-Aldrich ...
-
bioRxiv - Molecular Biology 2024Quote: ... then transferred to a Phase Lock Gel (PLG) tube (QuantaBio, Beverly, MA, USA) containing 0.5 mL phenol:chloroform:isoamyl alcohol (25:24:1) buffer (Merck). After thorough vortexing ...
-
bioRxiv - Neuroscience 2019Quote: ... Membranes were blocked in 5% milk in TBS-T and incubated overnight at 4°C with the primary antibodies anti-tyrosine hydroxylase (1:1,000; AB152; Merck Millipore) or anti-dopa decarboxylase (1:500 ...
-
bioRxiv - Genetics 2021Quote: ... Blots were washed thrice with TBS-T and visualized on a LAS4000 Fuji imaging system after incubating with Immobilon Western Chemiluminescent HRP substrate (Merck). The following antibodies were used ...
-
bioRxiv - Systems Biology 2020Quote: The constructs containing the TCA cycle enzymes fused to the biotin ligase and a FLAG tag were stably integrated in the Flp-In T-REx HeLa cells using the X-tremeGENE 9 DNA Transfection Reagent (Merck) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... the membranes were incubated with the respective HRP-conjugated secondary antibody in 5% dry milk powder in TBS-T for 1h at room temperature washed three times in TBS-T and imaged on an ECL ChemoCam 3.2 Imager (Intas) using Immobilon Forte Western HRP substrate (Merck Millipore). For pulldown of gH-V5 or gH-V5/ gL-Flag complexes ...
-
bioRxiv - Microbiology 2022Quote: ... Hydrolysis of 44 soluble non-chromogenic monoester substrates (Table S2) and T-2 mycotoxin (Merck Life Science S.L.U., Madrid, Spain) was assayed at 37ºC using a pH indicator assay with Phenol Red and monitored at 550 nm (46) ...
-
bioRxiv - Cell Biology 2023Quote: ... membranes were washed 3x with TBS-T and subjected to chemiluminescence detection with Immobilon Western Chemiluminescent HRP substrate (Merck Millipore) using a Gel-Doc XR+ System (Bio-Rad) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Sections were rinsed with PBS-T and once with deionized water before mounting with FluorSave mounting medium (Merck-Milipore, Germany) and coverslipping ...
-
bioRxiv - Neuroscience 2023Quote: ... washed in TBS-T and incubated with horseradish peroxidase–conjugated anti-rabbit (1:2000, #7074P2, Cell Signalling or 1:80000, #A0545, Merck) and anti-mouse (1:2000 ...
-
bioRxiv - Cell Biology 2024Quote: ... Secondary antibodies (table 1) were added in 5% milk TBS-T and membranes were developed using ECL luminol kit (Merck) and chemiluminescence films (Amersham Hyperfilm ECL ...
-
bioRxiv - Neuroscience 2019Quote: ... 25 U/ml Penicillin and 25 µg/ml Streptomycin and 0.455% (v/v) 7.5% NaHCO3 aqueous solution) on Millicell Cell Culture Insert (Merck) at air-medium interface ...
-
bioRxiv - Microbiology 2021Quote: ... 25 mM Tris-HCl pH 7.4) supplemented with protease inhibitors (Protease Inhibitor Cocktail set III, EDTA free, Merck) and PhosSTOP phosphatase inhibitors (4906845001 ...
-
Adipose Triglyceride Lipase protects the endocytosis of renal cells on a high fat diet in DrosophilabioRxiv - Cell Biology 2020Quote: ... pericardial nephrocytes were incubated for 30 min at 25°C in Schneider’s Medium with 0.5 mg/ml delipidated BSA (A9205, Merck) and 10 μM BODIPY FL C12 green fluorescent fatty acid ...
-
bioRxiv - Plant Biology 2019Quote: ... 0.8 % phytoagar (Duchefa) and 10 µg mL-1 Glufosinate-ammonium or 25 µg mL-1 hygromycin B (Merck) for herbicide selection ...
-
bioRxiv - Immunology 2020Quote: ... Primed neutrophils were obtained by treating the cells for 25 mins with 10 μM cytochalasin B (Merck, #C6762) and 100 nM N-Formylmethionyl-leucyl-phenylalanine (fMLF ...
-
bioRxiv - Molecular Biology 2022Quote: ... The recovered DNA solution was subjected to neutral phenol:chloroform:isoamyl alcohol 25:24:1 (pH 7.7-8.8, Merck/Sigma-Aldrich) extraction and ethanol precipitation ...
-
bioRxiv - Molecular Biology 2022Quote: ... the mixture extracted with an equal volume of neutral phenol:chloroform:isoamyl alcohol 25:24:1 (~75 μl; pH 7.7-8.8; Merck/Sigma-Aldrich), as described earlier ...
-
bioRxiv - Cancer Biology 2023Quote: ... Caps were rinsed with 20 µL of RIPA buffer (#89900, Pierce) containing 25 units of Benzonase (E1014, Merck) to collect any remaining tissue and briefly centrifuged ...
-
bioRxiv - Microbiology 2023Quote: ... The remaining RNA was combined with 25 μl of Magna ChIP Protein A+G magnetic beads (Merck Millipore) coated in 5μl of anti-m6A antibody (Merck Millipore ...
-
bioRxiv - Neuroscience 2023Quote: ... D-glucose (25 mM, Normapur) and 10 ng/mL of PDGF-AA (Platelet Derived Growth Factor-AA: Merck) and FGF-2 (Fibroblast Growth Factor-2 ...
-
bioRxiv - Molecular Biology 2024Quote: ... The bound complexes were eluted in lysis buffer 2 containing 25 mM glutathione or 5 mM biotin (Merck), respectively ...
-
bioRxiv - Molecular Biology 2024Quote: ... Cell lysates (25 µL) and bovine serum albumin (BSA) protein standards (0-1 mg/mL BSA (Merck, Germany) in resuspension buffer ...
-
bioRxiv - Biochemistry 2021Quote: ... The buffer of the anti-PTP1B antibody was exchanged to PBS-T using a Microcon-30kDa centrifugal filter unit (MRCF0R030, Merck Millipore) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: ... Th1 cell differentiation was induced by culturing CD4 T cells with antigen presenting cells treated with 50μg/ml mitomycin C (Merck, Darmstadt, Germany) in the presence of 1μg/ml OVA323-339 (Peprotech ...
-
bioRxiv - Developmental Biology 2020Quote: ... membranes were washed 3 times with TBS-T and subjected to chemiluminescence detection with Immobilon Western Chemiluminescent HRP (Horseradish Peroxidase) Substrate (Merck Millipore) using a Gel-Doc XR+ System (BioRad) ...
-
bioRxiv - Bioengineering 2022Quote: ... samples were washed 3× five minutes in BSA-T and incubated with an antibody against the FLAG-tag (#F1804 Sigma-Aldrich, now Merck, or #14793 Cell signaling ...
-
bioRxiv - Cell Biology 2023Quote: ... Blots were then washed with TBS-T three times for five minutes and exposed to Immobilon Western Chemiluminescent HRP substrate (Merck Millipore) according to product instructions for 30 seconds before being developed ...
-
bioRxiv - Immunology 2024Quote: ... Primary human T-cells were isolated from peripheral blood (NHS Blood & Transplant Service, UK) using Ficoll-PaqueTM (Merck Group, Darmstadt, Germany) density gradient centrifugation followed by positive selection using antibody-coated magnetic beads directed against either human CD4 or CD8 antigen (Miltenyi Biotechnology ...
-
bioRxiv - Genetics 2022Quote: ... 25 μg chromatin was diluted with IP dilution buffer and pre-cleared with Protein A agarose beads (Merck-Millipore) for 1 h at 4°C ...
-
bioRxiv - Biochemistry 2022Quote: The protein stock solutions were transferred to a deuterated buffer containing 35 mM potassium phosphate and 25 mM NaCl in D2O at a pD of 7.5 using 0.5 mL Centricon devices (Merck Millipore) with a MWCO of 30 kDa by washing and centrifuging several times at 10,000 x g and 4°C ...
-
bioRxiv - Biophysics 2020Quote: ... and 25 µL of a 10 vol% solution of tetraethyl orthosilica (TEOS, reagent grade, 98%) in ethanol (absolute, Merck) 60 ...
-
bioRxiv - Developmental Biology 2019Quote: ... Fixation of samples for SEM was done with Karnovsky fixative (2.5% glutaraldehyde (25% EM grade, Agar Scientific Ltd.) + 2% paraformaldehyde (PFA, Merck) in 0.1 M sodium cacodylate (Merck) ...
-
bioRxiv - Biochemistry 2020Quote: ... ammonia (25% in H2O), acetic acid (≥ 98.5%), ethanol (≥ 99.5%), and sodium hydrogencarbonate (NaHCO3, ≥ 99.5%) were purchased from Merck (Darmstadt, Germany). Tris(hydroxymethyl)amino-methane (TRIS ...
-
bioRxiv - Microbiology 2019Quote: ... An inoculum from the SDA plate was suspended in 25 ml of Sabouraud dextrose broth (SDB; Merck, Darmstadt, Germany) and incubated for 18 h under agitation (120 rev/min ...
-
bioRxiv - Cell Biology 2020Quote: ... followed by immediate addition of 70μl of the extraction solvent (LC-MS methanol 75 %: LC-MS acetonitrile 25%, Merck, containing labelled standards ...
-
bioRxiv - Immunology 2022Quote: ... The catalytically-inactive SPP D219A mutant was created using overlap-extension PCR (25) with KOD Hot Start Polymerase (Merck) and appropriate primers ...