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Citations for Merck :
1 - 50 of 223 citations for Strong Cation Exchange Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... followed by 2 layers of SCX strong cation exchange Empore™ SPE Disks (Merck). Samples were then lyophilized.
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bioRxiv - Cancer Biology 2022Quote: ... (2005).21,22 A strong cation exchanger (Ion exchanger I, Merck, Darmstadt, Germany) was used to prepare the tributylammonium salts of the nucleotides ...
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bioRxiv - Cell Biology 2022Quote: ... MAP-free neurotubulin was purified by cation-exchange chromatography (EMDSO, 650M, Merck) in 50mM PIPES ...
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bioRxiv - Immunology 2023Quote: ... cation exchange chromatography using Fractogel® EMD SO3-(S) resin (Merck Millipore, US) for intermediated purification ...
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bioRxiv - Cell Biology 2022Quote: ... MAP-free neurotubulin was purified by cation-exchange chromatography (EMD SO, 650 M, Merck) in 50 mM Pipes ...
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bioRxiv - Biophysics 2023Quote: ... MAP-free neurotubulin was purified by cation-exchange chromatography (EMD SO, 650 M, Merck) in 50 mM PIPES ...
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bioRxiv - Cell Biology 2019Quote: ... MAP-free brain tubulin was purified by cation-exchange chromatography (Fractogel EMD SO, 650 M, Merck) in 50 mM PIPES ...
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bioRxiv - Biophysics 2021Quote: ... we obtained microtubule-associated protein (MAP)-free neurotubulin by cation-exchange chromatography (1.16882, EMD SO3, Merck, Darmstadt, Germany) in 50 mM PIPES ...
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bioRxiv - Molecular Biology 2019Quote: ... The membranes were stripped using a 1x ReBlot Strong Antibody Stripping Solution (Merck) for 15 min ...
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bioRxiv - Cell Biology 2023Quote: ... Media containing high titre pseudo virus was collected at 4 h intervals after the first media exchange and continuously for 48 h thereafter and filtered through a 0.45 μm PES filter (Merck, SLHP033RS) to remove cell debris ...
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bioRxiv - Biochemistry 2021Quote: ... incubated with ReBlot Plus strong antigen retrieval agent (Merck, Watford, UK, cat. no. 2504) for 15 min ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were stripped with 1X ReBlot Plus Strong Antibody Stripping Solution (#2504, Merck Millipore), blocked for 30 minutes in blocking milk ...
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bioRxiv - Molecular Biology 2019Quote: ... The proteins were again diluted in 9 volumes of Exchange Buffer 2 (Exchange Buffer 1 with 200 mM NaCl) and concentrated with 30 MWCO Amicon Ultra-15 (Merck).
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bioRxiv - Microbiology 2023Quote: ... the protective effect of Mg2+ cations were added to assay buffers as MgSO4 (Merck) at CF physiologically relevant levels (0.5 and 1 mM ...
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bioRxiv - Molecular Biology 2024Quote: ... HSPB7 constructs were loaded to HiTrap Q anion exchange column (Merck) and eluted with a gradient of 150-200 mM NaCl in the elution buffer ...
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bioRxiv - Microbiology 2022Quote: ... The SARS CoV-2 glycoproteins were then purified by divalent cation affinity chromatography using TALON resin (Merck) or HiTrap IMAC FF followed by size exclusion chromatography using a Superose 6 Increase 10/300 column linked to an AKTApure instrument (Cytiva) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... a buffer exchange to 0.1% formic acid (Merck Millipore, Burlington, MA, USA) was performed using centrifugal molecular cut-off filters (Merck Millipore ...
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bioRxiv - Molecular Biology 2022Quote: ... Buffer exchange was performed using Amicon Ultra-15 centrifugal filter units (Merck) to have the purified proteins in mTOR wash buffer containing 25 mM HEPES (pH 7.4) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The lysate was loaded onto a Fractogel DEAE anion exchange column (Merck). PTE elutes in the flow-through ...
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bioRxiv - Immunology 2020Quote: ... Rabbit anti-human CD11c (ab216655) underwent buffer exchange using 50kDa amicon filters (Merck) so as to place antibodies in 0.1M NaHCO3 ...
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bioRxiv - Microbiology 2022Quote: ... Buffer exchange was performed via centrifugal filters (Amicon® Ultracel®-30K, Merck) using buffer A supplemented with 30% glycerol and 5 mM DTT ...
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bioRxiv - Immunology 2023Quote: ... and anion exchange chromatography using Eshmuno® Q resin (Merck Millipore, MA, US) for polishing ...
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bioRxiv - Genomics 2023Quote: ... Plating of 50 µL of bacterial cultures with an OD595 of 0.08-0.1 (corresponding to 0.5 Mc-Farland) was performed on cation-adjusted MH (CAMH) agar (Merck KGaA, Germany) with colistin sulphate ...
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bioRxiv - Microbiology 2023Quote: AST was performed using broth microdilution according the EUCAST guidelines (v.4.0) in cation adjusted MullerHinton broth (MH2, 90922, Merck) in duplicates ...
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bioRxiv - Microbiology 2021Quote: ... Bacterial inoculums were subsequently prepared using the direct colony suspension method.40 Three to five bacterial colonies were obtained from the agar plate and inoculated into an Eppendorf tube containing 1 ml of cation-adjusted Muller-Hinton broth (caMHB, Merck), consisting of 20-25 mg/L calcium ions (Ca2+ ...
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bioRxiv - Plant Biology 2020Quote: ... strand exchange is initiated by adding 3 mM ATP and 3.1 μM SSB (Merck). Aliquots are stopped at indicated times by addition of 12 μM EDTA and 0.8 % SDS ...
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bioRxiv - Immunology 2020Quote: ... 1X-PBS buffer exchange was achieved using Amicon® Ultra centrifugal filters (Merck Millipore) through a 30-kD membrane according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... media was replaced with fresh media containing 50µM Biotin (Merck) and the cells were incubated for a further 24 or 6 hours (for BirA*-RAB18 and BioID2(Gly40Ser)-SEC22A experiments respectively) ...
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bioRxiv - Biophysics 2019Quote: ... for 2 h and buffer exchange with centrifugal filter devices (10,000 MWCO, Amicon, Merck Millipore) into storage buffer ...
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bioRxiv - Neuroscience 2022Quote: ... α-syn was enriched by ion-exchange chromatography with the use of Fractogel-TMAE (Merck) and MonoQ (Cytiva ...
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bioRxiv - Immunology 2023Quote: ... The purified mAbs were subsequently subjected to buffer exchange and concentration with AmiconUltra (Merck #36100101).
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bioRxiv - Biochemistry 2023Quote: ... Buffer exchange to 1xHBS and protein concentration was conducted by ultrafiltration (Amicon Ultra 10 kDa MWCO, Merck).
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bioRxiv - Immunology 2023Quote: ... Iodixanol was removed through buffer exchange in a 100 kD Amicon ultra centrifugal filter unit (Merck Millipore). Titers of AAV viruses were determined through transducing HEK293T cells of diluted viruses.
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bioRxiv - Cell Biology 2023Quote: ... stably expressing the reverse tetracycline-controlled transcription activator rtTA-M2 and containing a locus for Flp-recombinase-mediated cassette exchange 55,56 were cultured in Dulbecco’s Modified Eagle’s Medium (Merck) supplemented with 10% FCS (Bio & Sell ...
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bioRxiv - Bioengineering 2021Quote: ... the buffer exchange was done using Amicon Ultra 0.5 ml 30K Centrifugal filters (Merck Millipore Ltd, Cork, Ireland). After 24 h of digestion ...
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bioRxiv - Bioengineering 2019Quote: ... Media consisted of DMEM:F12 (Merck) containing 10% FCS (Thermofisher Scientific) ...
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The intestinal circadian clock drives microbial rhythmicity to maintain gastrointestinal homeostasisbioRxiv - Physiology 2022Quote: ... DPX new mounting media (Merck) was added to preserve the tissues ...
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bioRxiv - Physiology 2023Quote: ... DPX new mounting media (Merck) was added to preserve the tissues ...
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bioRxiv - Cancer Biology 2019Quote: ... the culture media was replaced by media with/without Doxycycline (Dox; 1 µg/ml; Merck). Cells were lysed after 72h and monitored with a dual luciferase assay system (Berthold ...
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bioRxiv - Immunology 2020Quote: ... Fractions containing RBD-BAP were concentrated and the excess imidazole removed by buffer exchange using an Amicon 10kDa (Merck). RBD-BAP was biotinylated using E ...
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bioRxiv - Bioengineering 2023Quote: ... to exchange ammonium sulfate for phosphate buffer solution (Potassium Phosphate Monobasic Anhydrous (KH2PO4; EM Science, Merck KGaA, Darmstadt, Germany) + Sodium Phosphate Dibasic Anhydrous (Na2HPO4 ...
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bioRxiv - Cancer Biology 2020Quote: ... media was replaced with media containing 10 nM 5-Bromo-2′-deoxyuridine (BrdU) (Merck, B5002-100MG). Coverslips were then fixed ...
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bioRxiv - Microbiology 2023Quote: 1 mL overnight autoinduction media (Merck) was used for the inoculation of single colonies obtained from the transformation ...
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bioRxiv - Plant Biology 2019Quote: ... and fluorescence emission spectra were recorded before (t0) and 60 min (t60) after exchange of solution to either HBSS with 100 µM (±)-ABA (Merck) and 0.1 % EtOH (treatment ...
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bioRxiv - Biochemistry 2021Quote: ... Buffer exchange to PBS and the concentration of the protein were done by using amicons® (Merck Millipore Ltd, cat:UFC900324).
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bioRxiv - Biophysics 2020Quote: ... Further concentration and buffer exchange to 20 mM Tris-HCl pH 8.0 were carried out with 10kDa Amicon®Ultra-4 centrifugal filters (MERCK). The mixture of tetramers was then purified by anion exchange chromatography (MonoQ 5/50 GE Healthcare Life Sciences ...
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bioRxiv - Biochemistry 2020Quote: ... Strep-GlnA1 was eluted in the presence of 2.5 mM D-Desthiobiotin (IBA, Göttingen, Germany) followed by buffer exchange using an amicon centrifugal filter (30KDa) (Merck; Darmstadt, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... the Strep-Tactin eluate was diluted to a final salt concentration of 150 mM NaCl directly and loaded onto the Resource Q anion exchange column (Merck). The protein was eluted in 20mM HEPES pH 8.0 ...
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bioRxiv - Molecular Biology 2020Quote: ... GndHCl containing samples were subjected to a buffer exchange regime using Microcon-10 kDa centrifugal filter units (Merck, Darmstadt, Germany). Centrifugation (14’000x g ...
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bioRxiv - Microbiology 2021Quote: ... The eluted samples were subject to immediate concentration with concomitant buffer exchange with fresh PBS to remove imidazole using Amicon centrifugal filters (Merck). For hACE2 ...