Labshake search
Citations for Merck :
51 - 100 of 1382 citations for Streptavidin ZAP Antibody Kit rat since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... 50 μl/well HRP-coupled Streptavidin (0.25 μg/ml, Merck Millipore, Darmstadt, #OR03L) were added and incubated at RT for 1-2 hours ...
-
bioRxiv - Neuroscience 2021Quote: ... and antibodies were diluted with SignalBoost™Immunoreaction Enhancer Kit (Merck). Primary antibodies included phospho-TDP-43 (Ser409/410 ...
-
bioRxiv - Microbiology 2023Quote: ... the H-Tag Antibody HRP Conjugate Kit (Merck-Millipore, Darmstadt, Germany) was used according to the manufacturer’s protocol.
-
bioRxiv - Molecular Biology 2021Quote: ... rat anti-Ser2 Pol II (AB_11212363, Merck Millipore, 1:5000). Respective secondary antibodies ...
-
bioRxiv - Neuroscience 2021Quote: ... anti-Nestin mAB (clone rat-401,1:1000, Merck chemicals MAB353), anti-PDH mAb (E1α ...
-
bioRxiv - Physiology 2019Quote: For Immunobloting: anti-Ly108 (Rat, 3E11, Merck, Kenilworth, NJ, USA), anti-SAP (Rat ...
-
bioRxiv - Microbiology 2022Quote: ... and 15 µg/mL rat tail collagen (Merck, St.Louis, USA) pre-coated cell culture 6-well plate with EBM-2 endothelial media supplemented with 10% FBS and a growth factor bullet kit (Lonza ...
-
bioRxiv - Molecular Biology 2020Quote: ... Subsequently the bilayer is loaded with 60 μg/ml streptavidin (Merck Millipore, MA, USA) to saturation ...
-
bioRxiv - Biophysics 2020Quote: ... the streptavidin tetramer mixture was concentrated in a stirred 10kDa ultrafiltration cell (PBGC04310, MERCK). Further concentration and buffer exchange to 20 mM Tris-HCl pH 8.0 were carried out with 10kDa Amicon®Ultra-4 centrifugal filters (MERCK) ...
-
bioRxiv - Cell Biology 2022Quote: Snap-Streptavidin-WA-His (pETplasmid) was expressed in Rosettas 2 (DE3) pLysS (Merck, 71403). Culture was grown in TB medium supplemented with 30 μg/mL kanamycine and 34 μg/mL chloramphenicol ...
-
bioRxiv - Biophysics 2023Quote: Snap-Streptavidin-WA-His (pETplasmid) was expressed in Rosetta 2 (DE3) pLysS (Merck, 71403). Culture was grown in TB medium supplemented with 30 µg/ml kanamycin and 34 µg/ml chloramphenicol ...
-
bioRxiv - Biophysics 2022Quote: Snap-Streptavidin-WA-His (pETplasmid) was expressed in Rosettas 2 (DE3) pLysS (Merck, 71403). Culture was grown in TB medium supplemented with 30 μg/mL kanamycine and 34 μg/mL chloramphenicol ...
-
bioRxiv - Neuroscience 2019Quote: ... rat anti-mouse VCAM-1 (Merck, cat.# CBL-1300, 1:100), rat anti-mouse ICAM-1 (Abcam ...
-
bioRxiv - Biochemistry 2023Quote: ... HA clone 3F10 (rat monoclonal, conjugated to FITC, Sigma-Aldrich / Merck), GFP (mouse monoclonal ...
-
bioRxiv - Biophysics 2019Quote: ... the streptavidin tetramer mixture was concentrated in a stirred ultrafiltration cell (10 kDa cut-off, Merck). Further concentration and buffer exchange to 20 mM Tris-HCl pH 8.0 were carried out with Amicon Ultra-4 centrifugal filters (10 kDa cut-off ...
-
bioRxiv - Molecular Biology 2023Quote: ... Protein mixtures were incubated with 40μl PBST washed Streptavidin agarose resin (50% slurry, 69203-3 Merck) and incubated for 2h at room temperature on a roller ...
-
bioRxiv - Immunology 2023Quote: ... rat anti-hRIPK3 1H24 (1:1000; available from Merck-Millipore as MABC1640) were produced in-house ...
-
bioRxiv - Immunology 2023Quote: ... rat anti-hMLKL 10C222 (1:1000; available from Merck-Millipore as MABC1635), rat anti-hMLKL 7G222 (1:1000 ...
-
bioRxiv - Immunology 2023Quote: ... rat anti-hMLKL 7G222 (1:1000; available from Merck-Millipore as MABC1636), rat anti-hRIPK3 1H24 (1:1000 ...
-
bioRxiv - Immunology 2023Quote: ... rat anti-mMLKL 5A669 (1:1000; available from Merck-Millipore as MABC1634), rat anti-hMLKL 10C222 (1:1000 ...
-
bioRxiv - Molecular Biology 2021Quote: ... StrepII-tagged proteins (RBBP6, symplekin and WDR33) were detected using streptavidin-HRP conjugate (Merck Millipore, cat. No. 18152) and Amershan ECL Detection Reagents (Cytiva ...
-
bioRxiv - Bioengineering 2021Quote: ... Binding of biotinylated S1-RBD to coated VNAR-hFc was detected by streptavidin-HRP (Merck Millipore, 18-152). The signal intensities were collected for each primary VNAR-hFc antibody without competitor and compared to the signal when a competitor antibody was present ...
-
bioRxiv - Immunology 2023Quote: Immunoblotting was performed as described above and MIF-6xHis-biotin revealed using streptavidin-HRP-conjugate (Merck, Darmstadt, Germany), while MDL1-6xHis was revealed using a custom-made monoclonal primary antibody (1:10 dilution) ...
-
bioRxiv - Physiology 2020Quote: Insulin concentrations were measured by ELISA (EZRMI-13K Rat/Mouse insulin ELISA, Merck Millipore) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... was seeded in T25 flasks coated with rat tail type I collagen (Merck Millipore), in EndoGro-MV medium (Merck Millipore ...
-
bioRxiv - Cancer Biology 2020Quote: ... Secondary antibody incubation was followed by 0.1% TBST wash and developed using an ECL kit (Merck). Densitometry analysis was used for quantitation of protein expression levels using Image J ...
-
bioRxiv - Biophysics 2022Quote: ... Unconjugated streptavidin and Dpeg4-biotin were removed via ultrafiltration (Amicon Ultra-0.5, 100 kDa; Merck Millipore, Billerica, MA, USA). The detection media contained the prepared CF660R and Cy3-labelled detection antibodies for each antigen with final concentrations as follows ...
-
bioRxiv - Neuroscience 2023Quote: ... in samples where biotin-streptavidin amplification was used before blocking with 10% chemiblocker (Merck Millipore, Billerica, MA, USA, #2170) for 2 hours at room temperature ...
-
bioRxiv - Neuroscience 2024Quote: ... for 180 min prior to washing and development in Streptavidin-tertiary reagent (1:500 in 2% BSA and 0.1% Triton [TBS]) conjugated to either Cyanine 3 (Cy3) (Merck) or Alexa Fluor 488 (Life technologies ...
-
bioRxiv - Cell Biology 2019Quote: ... Collagen gels containing cells were prepared by mixing type I rat tail collagen solution (Merck) with 2 x concentrated DMEM (Merck) ...
-
bioRxiv - Cell Biology 2022Quote: ... ANA12 (10 μM [cells] or 1 μg [rats] 34,35; SML0209, Sigma-Aldrich/Merck group, USA) was dissolved in DMSO / Tween 80 / sterile 0.9% NaCl solution ...
-
bioRxiv - Developmental Biology 2023Quote: ... rat anti tyrosinated-α-tubulin 1:250 (MAB1864-I, clone YL1/2, EMD Millipore/Merck), mouse anti-Armadillo/β-Catenin 1:50 (N27A1 ...
-
bioRxiv - Immunology 2024Quote: ... 2010) that recognizes a common epitope on MASP-1,-3 and MAP-1 followed by HRP-conjugated streptavidin (Merck, RPN1231). The plates were revealed using TMB One as the substrate (Kementec ...
-
bioRxiv - Biophysics 2023Quote: ... adult male Wistar rats aged ∼ 5 weeks were given an intraperitoneal injection of crotaline (Merck, NJ) to induce pulmonary arterial hypertension ...
-
bioRxiv - Molecular Biology 2023Quote: ... 5% of input material was taken for further analysis and 150 μL of pre-washed Streptavidin Mag Sepharose® (GE28-9857-99; Merck) were added to the remaining material ...
-
bioRxiv - Neuroscience 2023Quote: ... Then, samples were incubated with streptavidin-phycoerythrin (30 min, RT) and before measurements on a Luminex MAGPIX® System (Merck-Millipore).
-
bioRxiv - Molecular Biology 2021Quote: ... and isolated murine hepatocytes (muHEP) were seeded on rat-tail collagen (10 µg cm−1, Merck Millipore) coated well plates at a density of 0.1×106 cells per cm² ...
-
bioRxiv - Neuroscience 2023Quote: ... after testing multiple antibodies (anti-NeuN rabbit Antibody, ABN78 & ABN78C3, Merck; anti-NeuN rabbit Antibody, ab177487, Abcam; anti-NeuN mouse Antibody, MAB377, Merck) and increasing antibody concentrations (up to 1:50) ...
-
bioRxiv - Plant Biology 2023Quote: ... Pulldowns were done as described17 with the following modifications: The biotinylated Aux/IAA7 DII peptide (AKAQVVGWPPVRNYRKN, N-terminal biotin) was bound to streptavidin-agarose beads (Merck, #16-126). 250 μl of total protein extract was combined with 45 μl of DII-beads and either 0 μM or 10 μM cvxIAA or 10 μM IAA ...
-
bioRxiv - Microbiology 2022Quote: ... The clear cell-free extracts were immunoprecipitated using monoclonal antibodies against T18 tag following Protein G Immunoprecipitation Kit (Merck Inc) protocol ...
-
bioRxiv - Cell Biology 2024Quote: ... Secondary antibodies (table 1) were added in 5% milk TBS-T and membranes were developed using ECL luminol kit (Merck) and chemiluminescence films (Amersham Hyperfilm ECL ...
-
bioRxiv - Pathology 2022Quote: ... trans-cardial perfusion was performed into the mice and rats with PBS followed by 4% paraformaldehyde (PFA, Merck). The kidneys were harvested and the samples were post-fixed in 4% PFA at 4°C for 90 min ...
-
bioRxiv - Pathology 2022Quote: ... respectively an anti-rat IgG conjugated to alkaline phosphatase (1:500) (Sigma-Aldrich®, Merck KGaA, Darmstadt, Germany), for 60 minutes ...
-
bioRxiv - Biochemistry 2021Quote: ... a biotinylated DNA oligonucleotide complementary to the target tRNA (Supplementary Table 7) was immobilized on Novagen Streptavidin Agarose resin (Merck-Millipore, Burlington, MA) in a 0.22 μm Ultrafree-MC filter cup (Merck-Millipore ...
-
bioRxiv - Cell Biology 2024Quote: ... Primary antibodies: anti-LC3 antibody Atg8 (Merck Millipore #ABC975) (1:1000) ...
-
bioRxiv - Immunology 2021Quote: ... was used for m6A immunoprecipitation (m6A-IP) using an m6A antibody (Synaptic, 202003) following the standard protocol of the Magna MeRIP m6A Kit (Merck Millipore). RNA was enriched through RNA Clean & Concentration-5 (Zymo Research ...
-
bioRxiv - Bioengineering 2021Quote: ... rats were sacrificed via injections of pentobarbital/phenytoin at 150 mg/kg pentobarbital (Beuthanasia®-D C IIIN, Merck Animal Health ...
-
bioRxiv - Neuroscience 2019Quote: ... the rats were treated with either 170,000 IU/kg IntronA (human recombinant interferon-alpha 2b, Merck Sharp & Dohme Limited), or saline (0.9% NaCl ...
-
bioRxiv - Cancer Biology 2022Quote: ... 400μL were mixed with 200μL of a 3 mg/mL collagen in 0.1% acetic acid solution (rat tail type I; Merck Millipore), followed by the addition of 10μL of 1 M NaOH ...
-
bioRxiv - Animal Behavior and Cognition 2023Quote: ... Each group was then further split into two subgroups depending on whether the rats received bicuculline (Merck, Darmstadt, Germany) or vehicle (artificial cerebrospinal fluid ...