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Citations for Merck :
51 - 100 of 136 citations for Small Volume Spectrophotometer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The tank water was spiked with an appropriate volume of the stock solutions of CdCl2 (Merck), CuSO4.5H2O (VWR Chemicals ...
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bioRxiv - Biochemistry 2020Quote: ... The indicated volume of sample was spotted onto TLC silica gel 60 T254 plates (Merck Millipore) and resolved using a mixture of 60 chloroform ...
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bioRxiv - Synthetic Biology 2021Quote: ... The sample was concentrated to a final volume of 500 μL in a 3kDa Centricon (Merck) by centrifugation at 5000 g ...
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bioRxiv - Neuroscience 2023Quote: ... Equal volumes (450 μL) of nuclei suspension and working solution (50% OptiPrep density gradient medium (Merck), 5 mM CaCl2 ...
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bioRxiv - Molecular Biology 2023Quote: ... 250 μg of proteins were emulsified with an equivalent volume of Freund’s Incomplete adjuvant (Merck F5506) and injected post 21 days ...
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bioRxiv - Evolutionary Biology 2023Quote: ... A small aliquot of each sample was added to Hemi medium17 with a 5-μl/ml antibiotic cocktail (P4083, Merck, Darmstadt, Germany) and incubated under dark conditions at 19–20 °C ...
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bioRxiv - Bioengineering 2019Quote: ... The optical density of the bacterial cultures was measured using a spectrophotometer (Spectroquant Pharo 100, Merck Millipore, India). A 450 W heat sealer (PFS-300P ...
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bioRxiv - Biochemistry 2020Quote: ... Platelet aggregation was elicited with 2 µM of γ-thrombin (Merck, Watford, UK, final volume 200 µL). Absorbance was monitored at 430 nm every 55 s for 35 min using an Optima plate-reader (BMG Labtech ...
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bioRxiv - Immunology 2024Quote: ... round bottom in a volume of 250 ul / well of RPMI media supplemented with Brefeldin A (Merck) at 1 ug/ul ...
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bioRxiv - Neuroscience 2021Quote: ... The supernatant was recovered and the protein concentration was estimated using the Direct Detect® Infrared Spectrophotometer (Merck-Millipore). 400 µg protein were incubated with 40 µL of streptavidin coupled DynabeadsTM M-280 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... the mixture extracted with an equal volume of neutral phenol:chloroform:isoamyl alcohol 25:24:1 (~75 μl; pH 7.7-8.8; Merck/Sigma-Aldrich), as described earlier ...
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bioRxiv - Genetics 2023Quote: ... cell surface protein samples were concentrated into 0.1 ml volumes using a 30 kDa centrifugal concentrator (Merck Millipore).
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bioRxiv - Developmental Biology 2021Quote: ... Purity of our mitotic sample was confirmed in a small sample cytospined (Simport, #720-1962) and immunostained with anti-pHH3 (Merck, #06-570, 1:500) and DAPI ...
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bioRxiv - Immunology 2022Quote: ... Tissue samples for RNA-protein extraction were cut into small pieces (< 5 mm) and placed in a microcentrifuge tube containing RNAlater® solution (Merck Ltd, Dorset, UK) and stored at −70°C until further use.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The residues in the vicinity (5.0 Å) of the small molecule CCX662 were optimized for the hydrogen bond network and minimized (cartesian minimization in Merck molecular force field, MMFF). VUF15485 was docked and scored using the ICM VLS scoring function (Neves et al. ...
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bioRxiv - Systems Biology 2022Quote: The samples for metabolomic analysis were run on a high-resolution mass spectrophotometer in Orbitrap Q Exactive Plus (Thermo) equipped with a SeQuant ZIC-pHILIC column (Merck) of dimensions 150 mm x 2.1 mm x 5-micron packing and in sequence with a ZIC-pHILIC guard column (Merck ...
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bioRxiv - Microbiology 2023Quote: The dissolved inorganic carbon concentration needed for the calculation40 was determined using the CaCO3 Merck Spectroquant kit (No. 1.01758.0001) and the Merck Spectroquant Pharo 100 spectrophotometer (Merck, Schaffhausen, Switzerland).
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bioRxiv - Cancer Biology 2020Quote: ... CM (and the same volume of SM as control) was then concentrated by 3 kDa Amicon filters (Merck-Millipore) to a final volume of 100 μl/mouse and stored at −20°C until usage ...
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bioRxiv - Cell Biology 2021Quote: ... and then equal volumes were concentrated 50-fold using Amicon Ultra-4 centrifugal filter units (Merck Millipore, Watford, U.K.). For analysis of cell-associated proteins ...
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bioRxiv - Systems Biology 2021Quote: ... Peptides were eluted in a final volume of 600 μL and filtered with 0.22 μm PVDF filters (Merck Millipore) before dried in a centrifugal vacuum concentrator ...
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bioRxiv - Plant Biology 2021Quote: ... The supernatant was recovered and four volumes of 0.1 M ammonium acetate in methanol (Merck gradient for liquid chromatography) were added ...
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bioRxiv - Immunology 2020Quote: ... the reduced peptide solution was purified five times using ZipTip Pipette Tips with C18 resin and 0.6 µl bed volume (Merck,) and eluted in 32.5% ACN/0.2% TFA ...
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bioRxiv - Molecular Biology 2020Quote: ... Proportional volumes of bacterial and yeast cells were mixed and filtered through 0.45 µm mixed cellulose membrane (Merck, Millipore) before transferring onto IM agar supplemented with 200 µM acetosyringone ...
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bioRxiv - Genomics 2022Quote: ... Starting culture volume was increased to 500 μl and 50 μl of 0.5 mg/mL lysostaphin (from Staphylococcus staphylolyticus, MERCK) was added ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions between 15.7 mL to 19.2 mL in elution volume were concentrated with Amicon Ultra-4 Centrifugal Filter Unit (10 kDa cutoff, Merck) to ∼2 mg/mL (measured by Bradford method) ...
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bioRxiv - Cancer Biology 2023Quote: ... They were further incubated with a 2× volume of 0.25× diluted TES buffer with a trace amount of benzonase nuclease (Merck) at 4°C for 45 min.
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was concentrated to 10% of the initial volume using a 100 kDa cutoff Amicon filter (Merck Millipore).
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bioRxiv - Immunology 2023Quote: ... Eluted IgG was diluted to a final volume of 500 µl with 1XPBS and then buffer exchanged in 50K centrifugal filters (Amicon Ultra-0.5 ml, Merck) for 10 min at 14000g and was finally resuspended in 100 µl of 1XPBS and treated as bulk IgG enriched from human plasma ...
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bioRxiv - Bioengineering 2023Quote: ... Medium was further concentrated to volumes <1 mL using 100kDa MWCO Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore). Tricorn 10/300 chromatography columns (Cytiva ...
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bioRxiv - Molecular Biology 2024Quote: ... The cell pellet was then lysed in 5X volumes of Lysis Buffer (300μL) [50mM Tris pH 8 (Merck, T6066), 150mM NaCl (Merck ...
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bioRxiv - Bioengineering 2021Quote: ... Virus was partially purified using depth filtration and anion exchange chromatography with syringes and small-scale filters (C0SP 23 cm2 [Merck] and Sartobind Q Nano [Sartorius]). Buffer compositions and other parameters were as previously described but scaled proportionately to the product and filter sizes (5) ...
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bioRxiv - Paleontology 2023Quote: An area of 1 cm² from bone artifacts was rubbed with a small swab (Foamtec UltraSOLV™ 1700 Series Swabs, Merck Life Science S.A.S, France) on a flat section of the artifact’s surface ...
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bioRxiv - Molecular Biology 2020Quote: ... The absorbance of the pink solution from each isolate was measured and recorded at 530 nm using spectrophotometer (Thermo Spectronic, Merck, SA).
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bioRxiv - Immunology 2021Quote: ... which contained as little saliva as possible were placed in a weighed Eppendorf tube and processed with 4x weight/volume of sputolysin working solution (Merck). Afterwards ...
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bioRxiv - Synthetic Biology 2021Quote: ... round bottomed deep well plate at 40 % liquid volume and sealed with a Breathe-easy sealing membrane (Merck, #2380059-1PAK). Overnight growth occurred in an Infors MT multitron pro shaker at 250 RPM rotating orbitally at 25 mm diametres at 37 °C for 17 h.
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bioRxiv - Microbiology 2021Quote: ... Equal volumes of total lysates were analyzed using SDS-PAGE and transferred to a polyvinylidene difluoride (PVDF) membrane (Merck Millipore). Rabbit anti-BMV 1a antiserum (1:10,000 dilution ...
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bioRxiv - Biochemistry 2022Quote: ... An assay volume of 150 μl was dispensed into a Hellma 10 x 2 mm Suprasil quartz cuvette (Merck #Z802778). For double-stranded substrates ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 ug inactive MEK1 (C-terminally His-tagged) in a total volume of 40 μL buffer (20 mM Hepes pH 8 (MERCK, Sigma Aldrich ...
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bioRxiv - Systems Biology 2022Quote: ... Protein precipitation was performed by addition of four volumes of ice-cold acetone (−20°C) (Merck Sigma, Cat. No. 650501) or TCA to a final concentration of 20% and proceeded for 1 hour at -20°C or 30 min at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... The elution volume of 60-70 mL was collected and the solution was concentrated to a volume of 200 μL with an Amicon Ultra-15 PLHK Ultracel-PL Membrane 100 kDa (Merck) spin column ...
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bioRxiv - Cancer Biology 2020Quote: ... The cell pellets were digested with a standard volume of suprapur nitric acid and hydrogen peroxide (Merck Millipore, MA, USA) (2:1 v/v ...
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bioRxiv - Plant Biology 2019Quote: ... 2.5 mM desthiobiotin [IBA]) and concentrated to a final volume of about 1 mL using Amicon Ultra-4 30 K filters (Merck). Purity of proteins was analyzed by SDS-PAGE using 10 % Mini-PROTEAN® TGX™ Precast Gels (BioRad ...
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bioRxiv - Biochemistry 2021Quote: ... Lipids containing [6-3H]GlcN-PI were dissolved in 100 μL of chloroform: methanol (2:1, by volume) and loaded to a high performance thin-layer chromatography (HPTLC) plate (Merck). The [6-3H]GlcN-PI was located by phosphorimaging ...
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bioRxiv - Plant Biology 2021Quote: ... Each MS medium was made by adding 1 bag of MS salt mix (Nihon pharmaceutical CO., LTD.) into the proper volume of milli-Q (Merck) water ...
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bioRxiv - Neuroscience 2021Quote: 400 μg of purified GABAAR mAbs were concentrated to a volume of 100 μl using Amicon Ultra 100 kDa columns (Merck) and then incubated with 40 μg CruzFluor™ 488 (CF488 ...
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bioRxiv - Genomics 2021Quote: ... The pellet was resuspended in PBS and the cells were then separated into three reactions: (1) added 1:20 volume of 1M NAI (03-310, Merck, 25 µl of NAI in 500 µl of infected cells ...
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bioRxiv - Microbiology 2023Quote: ... The volume of concentrated viral fractions (>50 kDa) was further reduced using Amicon Ultra 15 ml Centrifugal Filters (Merck-Millipore) prior to phenol/chloroform extraction ...
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bioRxiv - Evolutionary Biology 2023Quote: The frozen cell pellet was homogenized in RIPA buffer (Fujifilm, Osaka, Japan) containing 1/100 (v/v) in a final volume of Protease Inhibitor Cocktail (Merck) for 30 min at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... and 500 μg of chimeric InvP protein in-solution and mixing with an equivalent volume of Freund’s complete adjuvant (Merck F5881). For the subsequent five booster doses ...
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bioRxiv - Microbiology 2020Quote: ... The growth of each bacterial isolate was obtained by measuring the OD at 600 nm using a UV Spectrophotometer (Thermo Spectronic, Merck chemicals, SA). The control for this experiment consisted of inoculated broth without PEG 8000 ...