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Citations for Merck :
501 - 550 of 624 citations for Saporin Goat Polyclonal affinity purified Alexa488 labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... purified ParBΔCTD was concentrated by centrifugation in an Amicon Ultra-15 3-kDa cut-off spin filters (Merck) before being loaded into a Superdex-200 gel filtration column (GE Healthcare) ...
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bioRxiv - Cell Biology 2021Quote: ... antibody complexes were purified by incubation of lysate with Protein A Sepharose® beads (Merck, GE17-5138-01) for 3h rotating at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... for 2 hours at 37°C and purified using an Amicon 30 kDa MWCO filter (Merck, Darmstadt, Germany). Deep Vent exo-DNA polymerase (New England Biolabs ...
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bioRxiv - Biochemistry 2022Quote: ... Strep-Tactin purified mPSF complexes were concentrated in centrifugal filter (Amicon Ultra-15, MWCO 300 kDa, Merck Millipore) to approximately 0.5 mg·ml-1 ...
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bioRxiv - Molecular Biology 2022Quote: ... and SmWRKY27-GST were purified using a NucBuster™ Protein Extraction Kit (Cat. #71183; Merck KGaA, Darmstadt, Germany) for use in further EMSA experiments ...
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bioRxiv - Biophysics 2022Quote: Purified FHL was concentrated to 1-2 mg/mL using an Amicon centrifugal concentrator (100-kDa cutoff; Merck) and 3 µL of protein solution was applied to freshly glow-discharged C-flat 1.2/1.3 or 2/1 holey carbon grids (Protochips) ...
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bioRxiv - Cell Biology 2022Quote: ... coli cells using 0.2 mM IPTG for 4 hours at 30 °C and purified on flag beads (Merck) and eluted using flag peptide buffer (200 µg/ml flag peptide in 10 mM Tris-HCL ...
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bioRxiv - Biochemistry 2020Quote: ... the water used was type 1 water purified by a Milli-Q® water purification system from Merck Millipore (Billerica ...
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bioRxiv - Biochemistry 2021Quote: ... The eluted fractions containing purified protein samples were concentrated using a 10 kDa Amicon centrifugal filter (Merck Millipore) and the elution buffer suspending the purified protein was replaced with EDTA (10 mM)-PBS (1x ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The target γ-isomer was further purified by using LiChroprep RPI-18 (40-63 μm) (Merck-Millipore, Darmstadt) and subsequently lyophilized ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Mononuclear cell (MNC) fractions were purified by gradient centrifugation with Biocoll cell separation solution (Merck Millipore, Darmstadt, Germany). Leukemic cells were enriched by negative selection with a combination of CD3- ...
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bioRxiv - Biochemistry 2021Quote: ... Deionized water was purified using an in-house Synergy Type 1 Ultrapure Water System from Milli-QR (Merck). All solvents were HPLC graded (Fisher Scientific).
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bioRxiv - Plant Biology 2022Quote: ... Wash fractions containing AgLTP24 purified were applied to a 3K MWCO Amicon Ultra-15 centrifugal filter (Merck Millipore) for buffer exchange with NH4+-free FBM medium (FBM-) ...
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bioRxiv - Biophysics 2022Quote: ... Deionized water used for all experiments was purified with a Milli-Q Synthesis A10 Water Purification system (Merck). Absorbance spectra were recorded on a Cary50 UV/Vis spectrometer (Varian ...
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bioRxiv - Synthetic Biology 2023Quote: ... Purified protein was concentrated with the use of a 3K Amicon® Ultra 0.5 mL Centrifugal filter (Merck), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: The I-Ek-specific 14.4.4 mAb was purified from hybridoma supernatant using a protein A/G column (Merck) and gel filtration (Superdex-200 10/300 GL ...
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bioRxiv - Plant Biology 2023Quote: ... The instrument was filled with a solution of purified water (Milli-Q Plus; Merck Millipore, Billerica, MA, USA) which was degassed (1.0 × 5.5 Mini Module ™ ...
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bioRxiv - Cell Biology 2023Quote: ... the purified protein was dialyzed against 20 mM HEPES using an Amicon ultra-15 (Merck, Rahway, NJ, USA).
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bioRxiv - Cell Biology 2023Quote: Glass bottom well plates (Celvis) were coated with 5 µg/ml of human plasma fibronectin purified protein (Merck) for one hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: Glass bottom well plates (Celvis) were coated with 5 µg/ml of human plasma fibronectin purified protein (Merck) for one hour at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... the FAMEs were purified by preparative thin-layer chromatography (TLC) using a silica gel coated plate G60 (Merck) with dichloromethaneide as developing solvent ...
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bioRxiv - Cell Biology 2023Quote: ... Vector particles were purified on an iodixanol gradient and concentrated on Amicon Ultra-15 100K columns (Merck-Millipore). The AAV vectors were titrated as vg per milliliter by quantitative real-time PCR using ITR2 (inverted terminal repeats ...
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bioRxiv - Biochemistry 2024Quote: ... aeruginosa ATCC 27853 was purified from an overnight liquid culture using the GenElute Bacterial Genomic DNA Kit (Merck) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... The plates were then washed 5 times with HSWB followed by incubation of goat anti-mouse antibody (Merck, Germany) conjugated with HRP (diluted 1:3000 in LSWB + 1% BSA ...
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bioRxiv - Microbiology 2022Quote: ... HRP-conjugated anti-goat or anti-mouse IgG (dilution 1:1,000) were added and protein complexes were visualized using o-phenylenediamine (Merck). The absorbance was then measured at 490 nm employing PowerWave HT (Bio-Tek Instruments ...
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bioRxiv - Neuroscience 2019Quote: ... Slices were then permeabilized in blocking solution containing 5% normal goat serum (NGS, Sigma-Aldrich, Merck KGaA, Darmstadt, Germany) in 0.5% Triton for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were incubated in primary antibodies solution (antibody diluted in PBS containing 3% goat serum, 0.01% Triton x-100 (Merck)) at 4°C over night ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated for 24 h at +4°C with primary antibodies (goat anti-Chat (1:200, Merck, AB144P), mouse anti-GFAP (1:300 ...
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bioRxiv - Molecular Biology 2023Quote: ... This was followed by application of a Cy3-conjugated goat anti-mouse antibody (1:250 dilution, #AP124C; Merck Millipore) as the secondary antibody ...
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bioRxiv - Neuroscience 2024Quote: ... and then blocked for 1 h at RT with 0.2% Triton-X in PBS + 1% normal goat serum (NGS, Merck). Antibodies used and their concentration is found in Supplementary Table 4 ...
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bioRxiv - Bioengineering 2021Quote: ... all solutions were purified using Amicon centrifugal ultra-filtration devices (Amicon Ultra-2, Sigma Aldrich, 100 kDa membrane, Merck). Prior to use ...
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bioRxiv - Biochemistry 2020Quote: Freshly purified hASCT2 nanodiscs were concentrated to ∼1 mg ml−1 using an Amicon Ultra-0.5 mL concentrating device (Merck) with a 100 kDa filter cut-off and then 100 μM inhibitor was added and incubated for 1 h on ice ...
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bioRxiv - Biochemistry 2020Quote: ... The elution peak fraction of both purified samples was collected and concentrated using Amicon Ultra-10 centrifugal filter (10,000 MWCO, Merck) by centrifugation at 4000 rcf for 15 min ...
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bioRxiv - Cell Biology 2021Quote: ... Purified human integrin α5β1 and anti-α5 (MAB1956Z) and anti-α5β1 (MAB1999) antibodies were from Merck (Kenilworth, NJ, USA). Volociximab was from Novus Biologicals (Littleton ...
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bioRxiv - Cell Biology 2021Quote: ... Virotrap particles containing protein complexes were purified in a single step using biotinylated anti-FLAG BioM2 antibody (#F9291, Merck) and Dynabeads MyOne Streptavidin T1 Beads (#65601 ...
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bioRxiv - Microbiology 2021Quote: Polar metabolites were first extracted from the frozen SHIME-samples by means of ultra pure water (0,055 μS cm-1) obtained via a purified water system (VWR International, Merck). For this purpose ...
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bioRxiv - Biochemistry 2019Quote: ... Samples from England and Scotland were purified using a solid-phase extraction on 200 μL of 0.1 g/L microcrystalline cellulose suspension (Merck) in a 0.45 μm GHP filter plate (Pall Corporation) ...
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bioRxiv - Immunology 2020Quote: ... the reduced peptide solution was purified five times using ZipTip Pipette Tips with C18 resin and 0.6 µl bed volume (Merck,) and eluted in 32.5% ACN/0.2% TFA ...
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bioRxiv - Biochemistry 2021Quote: ... Purified ShTniQ was concentrated to 10 mg mL−1 using 10,000 molecular weight cut-off centrifugal filters (Merck Millipore) and flash-frozen in liquid nitrogen.
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bioRxiv - Biophysics 2020Quote: ... the purified mEGFP-tagged NbALFA was concentrated to 1 mg/ml using an Amicon Ultra 30K filter (Merck Millipore) and stored at -80 °C before use ...
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bioRxiv - Microbiology 2023Quote: ... The purified fractions were desalted and concentrated using an Amicon Ultra centrifugal filter unit (10-kDa cutoff; Merck Millipore), and the enzyme preparations were stored at −80°C ...
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bioRxiv - Developmental Biology 2022Quote: DYNLL1 and DYNLL2 antibodies were also purified using Amicon® Ultra 30K – 0.5 Centrifugal Filter Device (Merck Millipore Ltd). For antibody coupling using the Dynabeads™ Antibody Coupling Kit (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... DNA was purified by the drop dialysis method using the MF-Millipore Membrane Filter (Merck Millipore, Cat. No. #VCWP04700). Length distribution of the genomic DNA was analyzed by pulsed-field gel electrophoresis ...
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bioRxiv - Biophysics 2023Quote: The aqueous solutions of GQDs were purified utilizing dialysis kits and Amicon ultra-filtration devices (Amicon Ultra-15, Merck) with 1 and 3 kDa membranes ...
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bioRxiv - Plant Biology 2023Quote: ... Purified proteins were concentrated to 10–30 mg/ml by using a 10-kDa Amicon centrifugal filter device (Merck), flash-frozen in liquid nitrogen ...
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bioRxiv - Biochemistry 2024Quote: ... Purified MPP8 CTD was concentrated to 4.5 mg/ml using a 10-kDa cutoff centrifugal filtration device (Merck Millipore) and mixed with an equal volume (up to 0.5 µl ...
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bioRxiv - Developmental Biology 2021Quote: ... and incubated with FITC (Fluorescein isothiocyanate) conjugated secondary antibody (0.5 μg/ml of Goat Anti-Mouse IgG-FITC in 5%BSA) (GeNei, Merck, USA) (Cat.no ...
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bioRxiv - Physiology 2021Quote: ... with 0.2 % m/v Triton-X100 and 5% Normal Goat Serum (m/v) (S26-LITER, Merck Life Science UK LTD) (PBST.NGS ...
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bioRxiv - Microbiology 2020Quote: ... washed with PBS at room temperature before permeabilized for 30 min in PBS containing 0.1% Triton X-100 and 10% normal goat serum and incubated overnight at 4 °C in PBS containing 10% normal goat serum and primary antibodies: anti-Cytokeratin 14 (K14) antibody (SP53; Merck), anti-Neurofilament heavy polypeptide (NF200 ...
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bioRxiv - Neuroscience 2020Quote: ... followed by 2h incubation with Alexa Fluor-488 goat anti-rabbit IgG secondary antibody (1:500, A11008, Merck Millipore, MA). Slices were transferred to glass slides and covered with Fluoromount (Sigma-Aldrich ...