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Citations for Merck :
151 - 200 of 479 citations for SEAP ASSAY KIT since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... the Akt/mTOR phosphoprotein magnetic bead panel 11-plex 96-well plate assay (Merck Millipore, Darmstadt, Germany). This assay uses paramagnetic beads (6.5 μm diameter ...
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bioRxiv - Bioengineering 2022Quote: ... the activity assay of immobilised GalT consisted of 0.16 mM UDP Gal (Merck, 16mM GlcNAc (Sigma-Aldrich), 25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2022Quote: Stress assays (wounding or uraemic serum addition) were performed in Corning® 6-well plates (Merck, UK). The HDF ...
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bioRxiv - Microbiology 2020Quote: ... the end-point cell lysis staining assay consisted of adding Neutral Red dye (Merck KGaA, Darmstadt, DE) to cell monolayers ...
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bioRxiv - Cancer Biology 2021Quote: Cell proliferation was assessed by 3-(4,5-1,2methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (Merck Millipore). K562 cells (5,000/well ...
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bioRxiv - Genetics 2022Quote: Whole-cell protein lysates were extracted in modified radioimmunoprecipitation assay buffer supplemented with protease inhibitors (#5892970001, Merck) and phosphatase inhibitor cocktail (#4906837001 ...
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bioRxiv - Biochemistry 2023Quote: In situ protein-protein interaction was evidenced using Duolink® Proximity Ligation Assay (Merck, DUO920082, DUO92004, DUO92008). Cells were fixed with formaldehyde and permeabilized using ice-cold 90% methanol ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Respective ELISA kits (insulin, leptin, IL-6 – MilliPlex Map Mouse Adipokine kit, Merck, Germany ...
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bioRxiv - Cell Biology 2020Quote: ... and Click-iT® EdU (5-ethynyl-2’deoxyuridine) Assay (BCK-EDU488, baseclick GmbH, Merck/Sigma-Aldrich, UK), respectively ...
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bioRxiv - Microbiology 2020Quote: Cytotoxicity of the compounds were tested by use of a MTT [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide] assay (Merck) at a final MTT concentration of 0.45 mg/ml or by use of a ATPlite Luminescence Assay System (PerkinElmer ...
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bioRxiv - Genomics 2023Quote: Luminex assays were performed using the Milliplex Human Cytokine/Chemokine Magnetic Bead Panel (HCYTOMAG-60K-41, Merck Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Treatments for the LC3 assay included 3 hours with mTOR inhibitor Torin1 (Merck-Millipore, final concentration 250 nM), Bafilomycin A1 (Sigma ...
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bioRxiv - Immunology 2024Quote: ... For reporter gene assays 293FT cells were transiently transfected using a slightly modified polyethylenimine (PEI, Sigma-Aldrich/Merck) transfection method72 ...
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bioRxiv - Pathology 2021Quote: ... KAPA SYBR® FAST kit and qPCR kit were purchased from Merck (Darmstadt, Germany). RNeasy Mini kit and QIAcube were obtained from Qiagen (Venlo ...
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bioRxiv - Biochemistry 2023Quote: ... while an ApopTag Kit (Merck) was used to detect apoptotic cells in accordance with the manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: Radial 3D invasion assays were performed by embedding glioblastoma spheroids into a 50% Matrigel® solution (Corning®, Merck) (1:1 Matrigel® diluted in spheroids + GBM medium) ...
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bioRxiv - Cell Biology 2022Quote: ... For colony survival assays with BI2536 (Selleck-chemicals), talazoparib (Cat#S7048, SMS-Gruppen Denmark) or CPT (Cat#C9911, Merck), the methylcellulose medium with drug was shaken for more than 1 hour at 4°C before transfer into 6 well plates with 5 ml medium each well.
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bioRxiv - Molecular Biology 2019Quote: ... For gel assay reaction mixtures were loaded onto 12% SDS PAGE and then transferred onto PVDF membrane (Merck Millipore) and immunoblotted with antibodies as mentioned ...
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bioRxiv - Neuroscience 2022Quote: ... 50 μL/well of detection antibody (anti-FLAG-D2) diluted in Erenna Assay buffer (catalog #02-0474-00; Merck) at the final concentration of 1 μL/mL were added to the assay plate and incubated for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... The in situ proximity ligation assay (PLA) in combination with immunofluorescence microscopy was performed using the Duolink Detection (Merck) or the NaveniFlex (Navinci diagnostics ...
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bioRxiv - Neuroscience 2023Quote: ... For the example images for survival assay neurons were incubated with chicken polyclonal anti-MAP2 (1:2000; Merck Milipore) and secondary antibody Alexa Fluor 488 ...
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bioRxiv - Cancer Biology 2024Quote: ... reflecting cell-number and -viability) was assessed using the MTT [Thiazolyl Blue Tetrazolium Bromide] assay (Sigma/Merck, M5655-500mg) according to manufacturer’s instructions and sigmoidal dose-response curves were generated to calculate the mean IC50 values of each drug that were used in the subsequent study ...
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bioRxiv - Neuroscience 2024Quote: ... qPCR or EdU proliferation assay) for 72 h in the presence or absence of 200 nM Smoothened agonist (Merck). Full medium changes were performed daily ...
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bioRxiv - Cell Biology 2021Quote: ... the alkaline phosphatase detection kit (Merck) was used following manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... cell counting kit 8 (Merck, 96992) was applied for 2.5 h as recommended by the manufacturer.
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bioRxiv - Cell Biology 2021Quote: Cells were lysed and scraped in homemade Radioimmunoprecipitation assay buffer (RIPA) ((50 mM Tris pH 7.4, 150 mM NaCl, 1 % Triton X-100 (Merck, T9284), 2 mM EDTA ...
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bioRxiv - Biochemistry 2022Quote: ... An assay volume of 150 μl was dispensed into a Hellma 10 x 2 mm Suprasil quartz cuvette (Merck #Z802778). For double-stranded substrates ...
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bioRxiv - Immunology 2021Quote: ... and filtered through a 0.22 µM membrane The concentration of purified RBD was determined using the Bradford assay (Merck, Germany).
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bioRxiv - Biophysics 2021Quote: ... This mix included 98% unlabeled recombinant human E254N tubulin diluted in assay buffer containing oxygen scavengers (180 mg/ml catalase (C40, Merck) and 752 mg/ml glucose oxidase (22778.01 ...
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bioRxiv - Neuroscience 2022Quote: ... 100 μL/well of the MW1 antibody coupled with magnetic particles (appropriately diluted in Erenna Assay buffer catalog #02-0474-00; Merck), were added to the assay plate ...
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bioRxiv - Cancer Biology 2023Quote: Tumorspheres formation assay was performed by seeding transfected cells in Corning Costar ultra-low attachment plates (#CLS3473, Merck, Madrid, Spain) in DMEM F-12 medium (#11320033 ...
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bioRxiv - Neuroscience 2023Quote: The viability of SH-SY5Y cells following incubation with fibrils formed in the absence/presence of gangliosides was determined using a standard MTT cell proliferation assay (Merck).
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bioRxiv - Biochemistry 2024Quote: ... H2O was removed and ROS production was measured upon addition of a 100-μL assay solution which contained 10 μg/mL horseradish peroxidase (P6782, Merck), 10 mM luminol and the treatment (1 μM SCOOP ...
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bioRxiv - Molecular Biology 2023Quote: ... 2958 bp) was purified using commercial DNA purification kits (GenElute™ HP Plasmid Miniprep kit, Merck, #NA0160), according to standard procedures ...
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bioRxiv - Microbiology 2020Quote: ... IL-18 and TNFα were measured in serum samples using MILLIPLEX MAP Porcine Cytokine and Chemokine Magnetic Bead Panel - Immunology Multiplex Assay (Merck Millipore) following the manufacturer instructions ...
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Trypanosoma brucei J protein 2 functionally cooperates with the cytosolic Hsp70.4 and Hsp70 proteinsbioRxiv - Molecular Biology 2019Quote: ... 2 mM MgCl2) or into the appropriate assay buffer for functional studies and then subsequently concentrated against PEG 20000 (Merck, Germany). The protein yield was estimated using the Bradford assay (Sigma-Aldrich ...
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bioRxiv - Immunology 2019Quote: ... and viral titers were determined in Vero cells using a standard plaque assay at day 5 post-infection with crystal violet staining (Merck Millipore). The viral titers were determined in aliquots of harvested medium ...
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bioRxiv - Microbiology 2021Quote: ... Cytokine quantification was performed using a Human Cytokine/Chemokine/Growth Factor Panel A 48-Plex Premixed Magnetic Bead Multiplex Assay (Merck Millipore), using the Luminex MAGPIX System in 96-well plate format ...
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bioRxiv - Immunology 2021Quote: Cytokine and chemokine levels produced by PCLS after 2 dpi were assessed in a Multiplex assay in supernatants (dilution 1:2) with MILLIPLEX® Bovine Cytokine/Chemokine Panel 1 (BCYT1-33K-PX15, Merck) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: An immunofluorescence assay was performed in cryosections of rd10 animals using the following antibodies: rabbit polyclonal cone arrestin (1/5000, Merck Millipore), mouse monoclonal rhodopsin (1/1000 ...
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bioRxiv - Microbiology 2023Quote: ... The PLA assay was performed according to the manufacturer’s instructions using the In Situ Detection Reagents Red (Catalogue Number: DUO92008, Merck, Darmstadt, Germany).
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bioRxiv - Evolutionary Biology 2023Quote: ... The rest of the supernatant for each sample is used to measure the total protein level of the sample with a Bradford assay (Merck # B6916), following reagent protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... with a customized Milliplex kit (Merck Millipore).
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bioRxiv - Molecular Biology 2022Quote: ... and GenElute™ Plasmid Miniprep Kit (Merck), respectively ...
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bioRxiv - Cell Biology 2020Quote: ... we used Sigma-Aldrich kit from Merck-Sigma ...
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bioRxiv - Immunology 2023Quote: ... using Mouse Kapa Genotyping kit (Merck-Millipore), primers (500 nM ...
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bioRxiv - Microbiology 2023Quote: ... Nutrient specific Spectroquant kit (Merck Millipore, US) was used and the concentration was determined spectrophotometrically using a 96-well plate with a microplate reader (FilterMax F5 ...
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bioRxiv - Molecular Biology 2024Quote: Magna MeRIPTM m6A Kit (17-10499, Merck) was used to enable identification and transcriptome-wide profiling of m6A ...
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bioRxiv - Physiology 2021Quote: ... in maternal serum and placenta was performed with MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel – Immunology Multiplex Assays (MCYTOMAG-70K, Merck Millipore, Germany) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: Quantification of Th1/Th2 cytokines in supernatant of splenocytes was performed using mouse high sensitivity T cell magnetic bead panel assay (MHSTCMAG-70K, Merck, Darmstadt, Germany). 5×105 isolated splenocytes were co-cultured with different stimuli in 200 μL RPMI + 10% FBS ...