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Citations for Merck :
201 - 250 of 1512 citations for SARS CoV 2 Spike Glycoprotein S2 aa 800 1000 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... proteins were transferred Immobilon-E PVDF membrane (Merck Millipore, Tullagreen, Ireland) and AGAL was detected by monoclonal anti-Galactosidase alpha antibody (# ab168341 ...
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bioRxiv - Microbiology 2021Quote: ... and the supernatant was incubated with His-binding resin (Merck 69670-5) for 10-30 min at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... the supernatant was loaded onto a His-bind resin column (Merck Millipore) (1 ml ...
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bioRxiv - Plant Biology 2023Quote: ... and purified with Ni-NTA His•Bind® Resin agarose (Merck, 70666). The binding reaction was performed in 20 µL of binding buffer [10 mM Tris (pH 8.0) ...
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bioRxiv - Biochemistry 2023Quote: ... His tagged proteins were labeled using Ni-NTA-ATTO 488 (Merck Group) at a ratio of ATTO/protein = 1/500 in MN buffer (50 mM MES pH 6 ...
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bioRxiv - Molecular Biology 2019Quote: ... Samples were prepared by the addition of 800 µl cold perchloric acid (Merck, Darmstadt, Germany), to stop lactate dehydrogenase (LDH ...
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bioRxiv - Plant Biology 2020Quote: ... coli BL21 (DE3)pLysS competent cells (Novagen/Merck, Darmstadt, Germany). An overnight culture grown in dYT medium supplemented with 100 μg/ml carbenicillin and 34 μg/ml chloramphenicol was diluted to an OD600 of 0.1 with dYT supplemented with 100 μg/ml carbenicillin and grown at 37°C and 200 rpm to an OD600 of 0.6 ...
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bioRxiv - Cell Biology 2020Quote: Escherichia coli Rosetta (DE3) pLysS Competent Cells (Merck Cat# 70956) containing pBAD-thio-topo-LmaCK1.2-V5-His6 ...
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bioRxiv - Neuroscience 2023Quote: ... Lipopolysaccharide from Escherichia coli (LPS; 026:B6; catalogue # L8274 Merck Life Sciences UK ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were incubated with monoclonal anti-(c-Myc Tag) antibody (Merck Millipore) (HeLa extracts) ...
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bioRxiv - Physiology 2024Quote: ... sections were stained with hematoxylin and eosin (H&E; Merck, Zug, Switzerland).
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bioRxiv - Genomics 2020Quote: ... was incubated with/without 0.4μg human 6X-His-CDK9/Cyclin T1 (Merck, 140685) and/or 0.4μg human GST-tagged PP2A-α (Sigma ...
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bioRxiv - Plant Biology 2020Quote: ... His-tagged proteins were washed and purified using the Amicon Pro System (Merck) according to manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... The N-terminal tag was removed by an overnight incubation with thrombin (Merck). Undigested protein was removed by IMAC ...
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bioRxiv - Molecular Biology 2022Quote: ... incubated with the primary antibodies (see the list of antibodies in Table S2) and with an anti-rabbit secondary antibody (Merck), and visualized by chemiluminescence substrate (Merck ...
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bioRxiv - Evolutionary Biology 2020Quote: ... coli experiments were performed in LB (Merck 110285, Kenilworth, New Jersey) with the appropriate antibiotics and experiments with S ...
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bioRxiv - Biochemistry 2024Quote: ... coli BL21 (DE3) Codon plus-RIL cells (Novagen, Merck, Darmstadt, Germany). PDI proteins were purified to homogeneity by nickel affinity chromatography as described by Wang et al ...
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bioRxiv - Molecular Biology 2022Quote: ... Acetylation was evaluated with Western blot probed with anti-acetyl lysine antibodies (1:800 dilution) (Sigma/Merck). Mass spectrometry outsourced to Mass Spectrometry Laboratory IBB PAN ...
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bioRxiv - Molecular Biology 2023Quote: ... or HIS-Select Nickel Affinity Gel in a gravity-flow column (Merck, Darmstadt, Germany), followed by washing with binding buffer with and without 1.3% Triton X-100 ...
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bioRxiv - Biochemistry 2022Quote: ... and both His6 and GST tags were cleaved with 5-10 unit thrombin (Merck) per milligram of protein for 24 h at 4°C and subjected to the SEC using the superdex 200 10/30 GL column ...
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bioRxiv - Microbiology 2020Quote: ... coli onto a fresh plate of complex solid media (Merck LB 110283) containing selective antibiotics when appropriate ...
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bioRxiv - Biochemistry 2022Quote: ... Selenomethionine-labelled proteins were expressed in Escherichia coli B834(DE3) cells (Merck). All other proteins were expressed in Escherichia coli BL21(DE3 ...
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bioRxiv - Biochemistry 2022Quote: AniA was heterologously produced in Escherichia coli BL21(DE3) competent cells (Merck). The expression vector encoding the soluble domain of AniA (pET28A_aniA ...
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bioRxiv - Biophysics 2019Quote: ... coli MG1655 genome by PCR into pET15b (Merck Millipore, Billerica, MA, USA) by Gibson assembly (New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... coli by biochemical assays using Fluorocult LMX broth (Merck KGaA, Darmstadt, Germany) and API 20E strips (bioMerieux Inc. ...
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bioRxiv - Biochemistry 2022Quote: ... Canada) and transformed in Escherichia coli BL21 (DE3) (Novagen/Merck, Darmstadt, Germany) for protein production ...
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bioRxiv - Microbiology 2023Quote: ... coli) on filters with a pore size of 0.22 μm (Merck Millipore) for 24 hours at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... coli ESBL058 was inoculated into LB media (Lysogeny Broth, Merck, Darmstadt, Germany) and incubated until exponential phase at 37 °C at 180 rpm ...
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bioRxiv - Biochemistry 2019Quote: A CRISPR array containing three Mtb repeat sequences flanking two identical spacer sequences targeting the pUC19 multiple cloning site was assembled by BamHI digest of the double-stranded mtbCRISPR array oligonucleotide (Table S2) followed by ligation into pCDFDuet™-1 (Novagen, Merck Millipore). Mtb cas6 was cloned into MCS-2 to give pCRISPR.
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bioRxiv - Genetics 2021Quote: ... Proteins separated by SDS-PAGE were transferred onto a PVDF membrane (Immobilon-E, Merck) and blocked in 5% milk in Tris-Buffer Saline (TBS ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Paraffin sections (5 μm) were stained with hematoxylin/eosin (H&E; Merck, Darmstadt, Germany). For immunohistochemistry (IHC) ...
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bioRxiv - Immunology 2020Quote: ... followed by the addition of the TMB/E ELISA substrate (Merck Millipore, Billerica, MA). Absorbance was measured as described.
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bioRxiv - Neuroscience 2022Quote: ... slides were counterstained with 0.1% eosin for 30 s (H&E, Merck, Darmstadt, Germany), followed by dehydrating steps of an ethanol series (96% ...
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bioRxiv - Molecular Biology 2022Quote: ... Column chromatography was carried out using silica gel 60 (230-400 mesh, E. Merck) with the solvent system indicated in the procedures ...
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bioRxiv - Biochemistry 2022Quote: ... solution containing 150 µg of spike RBD was mixed with dye at a three-fold molar excess in the presence of NaHCO3 (Merck) buffer at pH 8.3 and incubated at 4 °C overnight ...
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bioRxiv - Bioengineering 2021Quote: ... The coding sequences for the His6-Tag were amplified with the pCOLADUETTM-1 (Merck/Millipore) plasmid backbone ...
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bioRxiv - Immunology 2021Quote: Mice received a single dose of LPS 0111:B4 from Escheria coli (Merck) at 1mg/kg in sterile PBS ...
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bioRxiv - Pathology 2023Quote: ... The resulting plasmid was transformed in Escherichia Coli BL21 (DE3) comnpetent cells (MERCK). Expression of TAT-Cre was induced growing transformed cells in LB Overnight Express™ Instant medium (Novagen ...
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bioRxiv - Cell Biology 2021Quote: ... Free floating brain sections and fixed astrocyte cultures were immunostained with the following antibodies (Table S2): Mouse anti-NeuN (1:100, Merck Millipore, Darmstadt, Germany), Rabbit anti-GFAP (1:200 ...
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bioRxiv - Immunology 2023Quote: ... The eluted His-Myc tagged recombinants were further concentrated using 3kDa ultra concentration units (Merck, Germany). The GST-tagged recombinant proteins were affinity purified using Glutathione Sepharose 4B beads and purified in batch mode ...
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Nerve pathology is prevented by linker proteins in mouse models for LAMA2-related muscular dystrophybioRxiv - Neuroscience 2022Quote: ... General histology was assessed after tissue fixation with 4% paraformaldehyde by H&E staining (Merck) or Picro Sirius Red stain (Direct Red 80 (Sigma ...
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bioRxiv - Developmental Biology 2023Quote: ... live imaging and tissue harvesting were performed under anaesthesia in 0.015% benzocaine (Merck E-1501) diluted in Vienna tap water ...
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bioRxiv - Biochemistry 2020Quote: ... Flag-tagged proteins were eluted by incubation with 150 μg/mL Flag tag peptide (Merck; F3290) with 0.1% digitonin in solubilisation buffer for 30 min at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... 1 mg 6xHis-tag purified Mif2-6xHis-6xFlag was immobilized on anti-FlagM2 agarose beads (Merck) for 1 h ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were eluted with 50 μl of 100 μg/mL of 3X FLAG tag peptide (Merck) in solubilization buffer followed by acetone precipitation (overnight ...
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bioRxiv - Developmental Biology 2021Quote: ... proSFTPC (1:1000; Merck, AB3786), mature SFTPC (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... anti-FAM21 (Merck, 1:1000), anti-phalloidin- TRITC (Sigma ...
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bioRxiv - Developmental Biology 2022Quote: ... SOX9 (1: 1000, Merck, AB5535) and β-Actin (1 ...
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bioRxiv - Cell Biology 2022Quote: ... anti-NFH (1:1000, Merck), anti-CCR7 (1:200 ...
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bioRxiv - Neuroscience 2020Quote: ... Stargazin (1:1000; Merck Millipore), GluN2B (1:1000 ...