Labshake search
Citations for Merck :
101 - 150 of 1045 citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... The cleared lysate was added to cOmplete His-Tag Purification Resin (Merck Millipore/Roche) and incubated at 4°C for 1.5 h ...
-
bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% (w/v) fetal calf serum (FCS) (Merck Millipore, Berlin, Germany) and 1% (w/v ...
-
bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% (w/v) fetal calf serum (FCS) (Merck Millipore, Berlin, Germany) and 1% (w/v ...
-
bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies used were anti-rabbit IgG (Fc) TRITC (Merck, # SAB3700846, 1:500) and anti-mouse IgG (Fc ...
-
bioRxiv - Cell Biology 2020Quote: Flp-In™T-REx™293 and HEK293 cell lines were grown under standard conditions (37°C, 5% CO2) in Dulbecco’s modified Eagle’s medium (Merck-Sigma-Aldrich, D5796). For Flp-In™T-REx™293 the medium was supplemented with 10% fetal bovine serum (FBS) ...
-
bioRxiv - Biochemistry 2022Quote: ... solution containing 150 µg of spike RBD was mixed with dye at a three-fold molar excess in the presence of NaHCO3 (Merck) buffer at pH 8.3 and incubated at 4 °C overnight ...
-
bioRxiv - Bioengineering 2021Quote: ... The coding sequences for the His6-Tag were amplified with the pCOLADUETTM-1 (Merck/Millipore) plasmid backbone ...
-
bioRxiv - Microbiology 2021Quote: ... approximately 50 - 100 L of groundwater was filtered sequentially through 0.2 µm and 0.1 µm pore sized PTFE filters (142 mm, Omnipore Membrane, Merck Millipore, Germany; Table S1). With the exception of H32 (did not yield sufficient volumes) ...
-
bioRxiv - Plant Biology 2022Quote: ... We mixed 0.1 μl template cDNA and 500 nM of each primer (Supplementary Table S1) with KAPA SYBR Fast qPCR Master Mix (Merck, Darmstadt, Germany). Each reaction comprised an initial denaturation step for 3 min at 95 °C ...
-
bioRxiv - Cancer Biology 2021Quote: ... consisting of 1% fetal calf serum (FCS; #S0615, Sigma, part of Merck, Darmstadt, Germany) in PBS ...
-
bioRxiv - Biophysics 2020Quote: ... After removal of the poly-E tag located on the “alive” subunit with 3C protease (MERCK) followed by a gel filtration step (Superdex-200 10/300 GE Healthcare Life Sciences) ...
-
bioRxiv - Biochemistry 2020Quote: ... Flag-tagged proteins were eluted by incubation with 150 μg/mL Flag tag peptide (Merck; F3290) with 0.1% digitonin in solubilisation buffer for 30 min at 4°C ...
-
bioRxiv - Biochemistry 2022Quote: ... 1 mg 6xHis-tag purified Mif2-6xHis-6xFlag was immobilized on anti-FlagM2 agarose beads (Merck) for 1 h ...
-
bioRxiv - Biochemistry 2021Quote: ... Proteins were eluted with 50 μl of 100 μg/mL of 3X FLAG tag peptide (Merck) in solubilization buffer followed by acetone precipitation (overnight ...
-
bioRxiv - Plant Biology 2022Quote: ... The filtered supernatant was loaded onto a column packed with cOmpleteTM His-tag purification resin (Merck). His6-proteins were eluted with 4x 2 bead volumes of elution buffer (20 mM Na HEPES ...
-
bioRxiv - Neuroscience 2022Quote: Double staining with Fluro Jade C and anti-SARS-COV2 Nucleocapsid antibody were performed by following the protocol from the Fluro Jade C supplier (AG325, Merck) with some minor modifications ...
-
bioRxiv - Molecular Biology 2021Quote: ... and goat anti-Human IgG (Fc specific)-Cy3 (1:1000 dilution in binding buffer; Merck). Finally ...
-
bioRxiv - Cell Biology 2019Quote: ... the membranes were first incubated with anti-T7-tag conjugated with alkaline phosphatase (1:10000, Merck, #6999) for 1 h ...
-
bioRxiv - Cancer Biology 2019Quote: ... GST was labeled with mouse Anti-GST Tag monoclonal antibody (05-782, Merck Chemicals; 2.5 μg/ml). To account for variations in the amount of fusion protein ...
-
bioRxiv - Cell Biology 2023Quote: ... to validate the gene expression the plasmid (vide-supra) was transfected into HEK293 cells using PEI (Sigma-Aldrich/Merck Cat. No. 49553-93-7) following an optimized version of the standard protocol(Rajendra ...
-
bioRxiv - Neuroscience 2024Quote: ... sections were blocked in 2 % goat or donkey anti-serum in KPBS and 3 % BSA plus 0.1 % Triton X-100 and incubated with primary antibody (rabbit anti-c-Fos, 1:200, Cell Signaling, Danvers, MA, USA; sheep anti-αMSH, 1:1000, Sigma-Aldrich, Merck KGaA, Darmstadt, Germany) for 1 h at room temperature or overnight at 4 °C ...
-
bioRxiv - Neuroscience 2021Quote: ... Microtubule-Associated Protein 2 (MAP-2) (Merck Millipore Burlington ...
-
bioRxiv - Cell Biology 2023Quote: ... 2% glucose + 2% D/L-lactate (Merck), or 2% D/L-lactate alone [31].
-
bioRxiv - Biophysics 2020Quote: ... expressing vimentin with a GFP tag were produced by lentiviral transfection (LentiBrite GFP-Vimentin Lentiviral Biosensor, Merck Millipore) of HeLa cells ...
-
bioRxiv - Microbiology 2020Quote: ... Mueller Hinton broth 2 (MHC-2, Merck, USA) for bacitracin susceptibility tests ...
-
bioRxiv - Immunology 2023Quote: ... 2 mM CaCl2 and 2 mM MgCl2 (Merck) to stop the fixation reaction.
-
bioRxiv - Immunology 2023Quote: ... 2 mM CaCl2 and 2 mM MgCl2 (Merck) and subsequently allowed to adhere on ICAM-1 coated glass slides before TOCCSL imaging ...
-
bioRxiv - Immunology 2023Quote: ... 2 mM CaCl2 and 2 mM MgCl2 (Merck)) ...
-
bioRxiv - Immunology 2023Quote: ... 2 mM CaCl2 and 2 mM MgCl2 (Merck). Cells were kept on ice or seeded onto ICAM-1-coated glass slides for imaging at 37°C and in TIRF mode ...
-
bioRxiv - Immunology 2023Quote: ... 2 mM CaCl2 and 2 mM MgCl2 (Merck) and afterwards incubated with excess amounts of mSav-cc-PS-CFP2 for 30 minutes on ice ...
-
bioRxiv - Immunology 2023Quote: ... 2 mM CaCl2 and 2 mM MgCl2 (Merck) and subsequently allowed to adhere on ICAM-1 coated glass slides prior to imaging in TIRF mode at 22.5°C ...
-
bioRxiv - Immunology 2023Quote: ... 2 mM CaCl2 and 2 mM MgCl2 (Merck)) ...
-
bioRxiv - Immunology 2023Quote: ... 2 mM CaCl2 and 2 mM MgCl2 (Merck)) and kept on ice until imaging proceeded ...
-
bioRxiv - Immunology 2023Quote: ... 2 mM CaCl2 and 2 mM MgCl2 (Merck)) prior to imaging.
-
bioRxiv - Immunology 2023Quote: ... 2 mM CaCl2 and 2 mM MgCl2 (Merck). Cells were allowed to adhere on ICAM-1 coated glass slides for 10 minutes at 25°C before fixation ...
-
bioRxiv - Immunology 2023Quote: ... 2 mM CaCl2 and 2 mM MgCl2 (Merck) prior to seeding the cells onto ICAM-1-coated glass-slides ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... were captured on the same chip by a previously immobilized anti-Fc antibody (Merck Life Science S.r.l). Anti-FLAG or anti-Fc antibodies were immobilized by classical amine coupling chemistry (29 ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Coding region of RBD-Fc was subcloned into a modified pCGS3 (Merck/formally known as Sigma-Aldrich) for glutamine selection in CHOZN GS-/- cells ...
-
bioRxiv - Cell Biology 2023Quote: ... the reducing agent 2 µl 2-picoline borane complex (PB, 2 M in DMSO, Merck) and 2 µl water ...
-
bioRxiv - Biophysics 2019Quote: ... the cleaved His-Tag (2kDa) was removed from the sfGFP-MinDD40AΔ10 (55kDa) solution by ultrafiltration using AmiconUltra-0.5 50k filters (Merck Millipore). Proteins in the storage buffer were stored at −80°C ...
-
bioRxiv - Bioengineering 2020Quote: ... and the membrane was incubated with primary antibody (anti-his tag, 1:1000, Merck Millipore, Merck KGaA, Darmstadt, Germany) diluted in 2 % skim milk for 1 hour ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cdk1 and Cdc25B cDNAs were subcloned in frame with the N-terminal His-tag into pRSETa (Novagen, Merck Biosciences). Cdc25B cDNA was also subcloned into pcDNA3 without (pCdc25B) ...
-
bioRxiv - Neuroscience 2023Quote: ... 2 mM CaCl2 and 2 mM MgCl2 (all Merck). The pH was adjusted to 7.3-7.4 (S20 ...
-
bioRxiv - Biophysics 2023Quote: ... 2-aminoethoxydiphenyl borate (2-APB) (Sigma-Aldrich/Merck, Germany), BL-1249 ...
-
bioRxiv - Molecular Biology 2020Quote: ... The Bruce-4 cell line was maintained in DMEM supplemented with 15% FCS and ESGRO (Merck Millipore, UK) on a 0.1% gelatine-coated dish at 10% CO2.
-
bioRxiv - Cancer Biology 2023Quote: ... Immunoreactive bands were visualised on Licor Odyssey Fc (Cambridge, UK) using enhanced chemiluminescence (ECL, Merck Millipore, Darmstadt, Germany). β-actin was used a loading control.
-
bioRxiv - Molecular Biology 2020Quote: ... WT—or its mutants fused with a His-tag on the amino-terminus—were expressed in Escherichia coli BL21 (DE3) using pET15b vector (Merck). A single colony was inoculated in 2 mL Luria-Bertani broth (LB ...
-
bioRxiv - Biochemistry 2021Quote: ... were constructed using standard genetic engineering techniques with a His6 tag at the N-terminus using the pET28a vector (Novagen, Merck). The recombinant CAHS1 protein ...
-
bioRxiv - Biochemistry 2021Quote: ... varieornatus CAHS1 protein was constructed and cloned as a fusion protein with a hexahistidine (His6) tag at the N-terminus using a pET28a vector (Novagen, Merck). CAHS1-N (Met1-Ser96 ...
-
bioRxiv - Microbiology 2022Quote: ... The clear cell-free extracts were immunoprecipitated using monoclonal antibodies against T18 tag following Protein G Immunoprecipitation Kit (Merck Inc) protocol ...