Labshake search
Citations for Merck :
601 - 650 of 733 citations for Recombinant human IgE kappa L His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... 70 L of groundwater were filtered onto a 0.1 μm pore-size PTFE membrane filter (Merck Millipore, Darmstadt, Germany). The flow-through was collected in a sterilized container ...
-
bioRxiv - Immunology 2020Quote: ... France) in 0.11 mol/L sodium acetate (pH 5.5) containing 0.003% (v/v) H2O2 (all from Merck, Darmstadt, Germany). The reaction was stopped with 2M H2SO4 (Merck) ...
-
bioRxiv - Physiology 2022Quote: ... and seeded on a glass-bottom dish (Iwaki Co. Ltd., Tokyo, Japan) with 0.01% poly-L-lysine (Millipore, Merck) coating ...
-
bioRxiv - Molecular Biology 2022Quote: All samples were centrifuged at 16,000 × g for 30 min and 400 µL supernatant was loaded on a 0.5 mL centrifugal ultrafiltration unit with 3 kDa cut-off membrane (Amicon, Merck) and concentrated down to 20 µL ...
-
bioRxiv - Microbiology 2022Quote: ... The dilutions were plated on nutrient broth agar (NA) plates containing 0.04% L-arabinose using a replica plater (Merck). After drying the plates were incubated for 16 h at 30°C ...
-
bioRxiv - Neuroscience 2023Quote: ... Neurons were plated (12-well plate) on glass coverslips coated with poly-L-lysin-hydrochloride (1 mg/ml, Merck). After 2h ...
-
bioRxiv - Microbiology 2023Quote: Seawater or groundwater (4-5 L) were filtered using a peristaltic pump onto polycarbonate membrane filters (0.1 or 0.2 µm, 47 mm, Merck, Israel) and stored at −20°C until extraction (n=1 per sample except in selected samples where n=4) ...
-
bioRxiv - Plant Biology 2023Quote: ... Next, calli were moved to callus regeneration media (CRM; per L: 4.43g of LS, 30g maltose (Sigma/Merck #M5885), 600μl CuSO4 (1mg/ml) ...
-
bioRxiv - Immunology 2023Quote: Isolated cells were transferred onto 18mm coverslips coated with 0.001% poly-L-lysine (Merck, cat: P4707-50ML, 10× stock) and incubated for 10 mins at 37°C to ensure cell attachment ...
-
bioRxiv - Microbiology 2023Quote: ... containing solid (7 g L−1 agar) full-strength Hoagland (2.75 mL well−1; pH adjusted to 6.5) (Sigma-Aldrich, Merck), sealed with parafilm and placed under the growth chamber ...
-
bioRxiv - Developmental Biology 2024Quote: ... consisting of 250 μL of HTF and 1 mM reduced L-glutathione (GSH, Sigma-Aldrich-Merck KGaA, Darmstadt, Germany). To reduce the female to female variability ...
-
bioRxiv - Cell Biology 2024Quote: ... medium—composed of 6.7 g Yeast Nitrogen Base without amino acids (Difco 291940) and 0.8 g complete supplement mixture drop-out (Formedium DCS0019) in 1 L—supplemented with 2% glucose (Merck). Cells from glycerol stocks or agar plates were inoculated into YPD and incubated at 30°C on an orbital shaker at 250 rpm (in an Innova 4230 incubator ...
-
bioRxiv - Neuroscience 2020Quote: ... RIPA and FA fractions was assessed using the Human Aβ1-42 enzyme-linked immunosorbent assay (ELISA) Kit (Merck) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2021Quote: Primary human macrophages (pMФ) were isolated from the whole blood of healthy volunteers with Biocoll separating solution (Merck) according to the manufacturer’s protocol and seeded at a density of 2×106 cells per well in a 6-well plate ...
-
bioRxiv - Biochemistry 2020Quote: ... Human Rab8a 6-176aa (referred to as Rab8a)-encoding DNA was cloned into a pet51b(+) vector (Merck Millipore), resulting in a construct with a N-terminal Strep® II tag and enterokinase cleavage site and a C-terminal 10xHis tag ...
-
bioRxiv - Biochemistry 2020Quote: ... at increasing concentrations (3.8 pM–250 nM) in the presence of 90% heat-inactivated human serum (H5667, Merck) and incubated at 4 °C overnight ...
-
bioRxiv - Microbiology 2021Quote: ... The human macrophage-like cell line U937 (ATCC no. CRL-1593.2) was cultured in RPMI-1640 medium (Merck) supplemented with 10% iFCS ...
-
bioRxiv - Cell Biology 2021Quote: Human hTERT RPE-1 cells (ATCC, CRL-4000) were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM/F12, Merck) supplemented with 10% fetal calf serum (FCS) ...
-
bioRxiv - Bioengineering 2022Quote: ... Human brain endothelial cell line (hCMEC/D3) and EndoGRO™ MV cell medium kit were purchased from Merck-Millipore (MA ...
-
bioRxiv - Cell Biology 2023Quote: Glass bottom well plates (Celvis) were coated with 5 µg/ml of human plasma fibronectin purified protein (Merck) for one hour at room temperature ...
-
bioRxiv - Cell Biology 2023Quote: Glass bottom well plates (Celvis) were coated with 5 µg/ml of human plasma fibronectin purified protein (Merck) for one hour at room temperature ...
-
bioRxiv - Genomics 2023Quote: Luminex assays were performed using the Milliplex Human Cytokine/Chemokine Magnetic Bead Panel (HCYTOMAG-60K-41, Merck Millipore) according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2023Quote: ... spermatozoa from JF1/MsJ males were capacitated in Human Tubal Fluid medium (Merck Millipore, Cat#MR-070-D) supplemented with 10 mg ml−1 Albumin (Sigma ...
-
bioRxiv - Immunology 2023Quote: ... Cells were cultured for 14 days in RPMI 1640 (ccpro) supplemented with 10 % heat-inactivated human serum (Merck), 10 ng/ml IL-7 ...
-
bioRxiv - Developmental Biology 2024Quote: ... spermatozoa were released into 500 μL of preincubated human tubal fluid medium (HTF: Millipore, Merck KGaA, Darmstadt, Germany) for 10 min at 37°C ...
-
bioRxiv - Cell Biology 2024Quote: ... cover slips were incubated with the primary antibody (SV40 T antigen mouse-anti-human, Merck-Millipore, 1:50) overnight at 4 °C in a humidifier chamber ...
-
bioRxiv - Evolutionary Biology 2020Quote: Larvae were raised as described above until 11 dpf and deeply anesthetized with 160 mg/l of Tricaine (Ethyl 3-aminobenzoate methanesulfonate salt, MS-222, Merck) before dissecting their pectoral fins ...
-
bioRxiv - Developmental Biology 2022Quote: Zebrafish and medaka samples from different developmental stages harbouring mutations in vsx genes were deeply anesthetized for 5-10 minutes with 160 mg/L of tricaine (ethyl 3-aminobenzoate methanesulfonate salt; MS-222; Merck) before dissecting their heads ...
-
bioRxiv - Plant Biology 2020Quote: Frozen cell pellets harvested from approximately 1 L of suspension cultures were crushed with quartz fine granules (Merck, Darmstadt, Germany) with a precooled mortar and pestle in ice-cold lysis buffer (25 mM Tris ...
-
bioRxiv - Biochemistry 2020Quote: ... Protein pellets were resuspended in 120 μL PBS containing 1% SDS and desalted by passing through Amicon Ultra 0.5 mL 10K cutoff desalting columns (Merck Millipore) equilibrated with 1% NP-40 ...
-
bioRxiv - Microbiology 2021Quote: ... cells were allowed to adhere in a 24-well plate with poly-L-lysine-coated glass coverslips for 1 hour (Merck). After infection with labelled A ...
-
bioRxiv - Biophysics 2022Quote: ... For the valine-labeled sample ketoisovalerate was added to a final concentration of 40 mg L-1 together with deuterated leucine (Merck) to a final concentration of 25 mg L-1 ...
-
bioRxiv - Neuroscience 2021Quote: ... Explants were cultured in 35 mm tissue culture dishes pre-coated with poly-L-lysine (20 µg/ml for 1 hr; Merck) and laminin (20 µg/ml for 1 hr ...
-
bioRxiv - Synthetic Biology 2020Quote: ... All culture media were supplemented with either 20 g L−1 of D-glucose or maltose (both from Merck Millipore).
-
bioRxiv - Genomics 2021Quote: ... infusion medium bacterized the day before use with Klebsiella pneumoniae and supplemented with 0.8 mg/L of β-sitosterol (Merck). Cultivation and autogamy were carried out at 27 °C ...
-
bioRxiv - Biochemistry 2021Quote: ... and plated on 35 mm (DIV7 neuron pulse labeling) or 60 mm (DIV5 and DIV14 neuron pulse labelling) dishes coated with poly-L-lysine (Merck) at a density of 1×105 and 2×105 cells/cm2 ...
-
bioRxiv - Biochemistry 2021Quote: ... Lipids containing [6-3H]GlcN-PI were dissolved in 100 μL of chloroform: methanol (2:1, by volume) and loaded to a high performance thin-layer chromatography (HPTLC) plate (Merck). The [6-3H]GlcN-PI was located by phosphorimaging ...
-
bioRxiv - Neuroscience 2021Quote: ... Cells were plated onto the 96-well plates coated with poly-L-ornithine (Sigma-Aldrich, Merck KGaA, St. Louis, MO). Equal volumes of cell suspension were plated onto the center of the dish ...
-
bioRxiv - Microbiology 2022Quote: ... 100 µL was added to dissolve the dried pellet before addition of 800 µL of 12.9 mM orcinol (Sigma Aldrich, Merck Life Science ...
-
bioRxiv - Biochemistry 2022Quote: ... as described by the manufacturer and dried down in a Speed-Vac concentrator (Thermo-Scientific) resuspended in 20 μl L/C water containing 3% acetonitrile (MeCN) (Merck) and 0.1% FA ...
-
bioRxiv - Neuroscience 2023Quote: ... followed by incubation with a rabbit anti-Kv4.3 primary antibody (1:10000, Alomone Labs) together with chicken (G)/mouse (L) anti-TH primary antibody (1:1000, Abcam / Merck) in a carrier solution (1% NGS ...
-
bioRxiv - Bioengineering 2023Quote: ... The pre-adipocytes were plated in custom-made plates (Supp. Fig. 15b) and cultured in growth medium -low glucose (1g l-1) DMEM (Merck) supplemented with 10% FBS (Merck) ...
-
bioRxiv - Immunology 2023Quote: ... L-Cystine dihydrochloride, RPMI 1640 medium (with sodium bicarbonate, without methionine, cystine and L-glutamine) and homoharringtonine were purchased from Merck. Fetal calf serum (FCS ...
-
bioRxiv - Cell Biology 2023Quote: ... the media of the cells was replaced with XF base media supplemented with 2 mM L-Glutamine (Merck Life science), 17.5 mM glucose (Fischer Scientific) ...
-
bioRxiv - Physiology 2023Quote: ... was used to slowly inject 200 μL of the injection mix composed of ∼2×1011 genome copies of AAV diluted in phosphate-buffered saline (P4417, Merck) containing 2.5 mM MgCl2 and 1 mM KCl ...
-
bioRxiv - Immunology 2024Quote: ... and passaged two times on MDCK-II cells with FBS-deficient medium containing 1% penicillin/streptomycin and 1 µg/ml of N-tosyl-L-phenylalanine chloromethyl ketone-treated trypsin (TPCK-trypsin, Merck KGgA ...
-
bioRxiv - Microbiology 2023Quote: ... tricornutum PT15 Xenic Minus nitrate (0 µM) 100 µL of an aqueous 15N- L-valine-solution (98 atom % 15N, Merck, Saint Louis ...
-
bioRxiv - Plant Biology 2024Quote: ... The assay was carried out by replacing the distilled H2O in the wells by a 100 µL reaction solution of 100 µM Luminol or L-012 (Merck), 20 µg mL-1 horseradish peroxidase (Merck ...
-
bioRxiv - Microbiology 2024Quote: ... individually rinsed in aseptic conditions and placed in enclosed sterile polycarbonate water tanks (AquaMedic, Germany) with 2.5 L of ultra purified water (Mili-Q, Merck, Germany) each ...
-
bioRxiv - Neuroscience 2024Quote: ... the myelinated slices were incubated 16 hours at 6 DIV in 0.5 mg/ml L-α-Lysophosphatidylcholine (LPC, 830071P, Avanti, Merck) added to fresh culture medium.