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Citations for Merck :
251 - 300 of 1127 citations for Recombinant Sortase Protein His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Protein was concentrated using Amicon filters (Merck, Darmstadt, Germany) in buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred onto Immobilon-P PVDF membranes (Merck) using a semi-dry blotting system (ATTO) ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred to a PVDF membrane (Merck Millipore) and blocked with 5% skimmed milk-TBST (TBS 10x [Sigma-Aldrich] diluted to 1x in distilled water containing 0.1% Tween 20 [Sigma-Aldrich] ...
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bioRxiv - Biochemistry 2023Quote: ... was used to plot elution profiles of the protein standard mix Supelco® 69385 Protein Standard Mix 15-600 kDa (Sigma-Aldrich/Merck, Darmstadt, Germany), and the analyzed protein samples.
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bioRxiv - Plant Biology 2020Quote: ... After transferring proteins from gels to PVDF membranes (Merck-Millipore) using Trans-Blot Turbo System (Bio-Rad) ...
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bioRxiv - Plant Biology 2020Quote: ... After transferring proteins from gels to PVDF membranes (Merck-Millipore) using Trans-Blot Turbo System (Bio-Rad) ...
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bioRxiv - Biochemistry 2021Quote: ... after which Protein G agarose/Salmon sperm beads (Merck Millipore) were added and incubated for 1 hour ...
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bioRxiv - Biophysics 2020Quote: ... The protein was concentrated using a centrifugal filter (Merck Millipore) and the concentration was determined by UV absorbance using the corresponding ε values (M-1 cm-1).
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bioRxiv - Molecular Biology 2021Quote: Proteins were transferred onto Immobilon-P PVDF membranes (Merck IPVH00010) using a wet-transfer system (Bio-Rad ...
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bioRxiv - Developmental Biology 2020Quote: ... the proteins were transferred to polyvinylidene fluoride (PVDF) membranes (Merck) that were activated in methanol (Carl Roth ...
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bioRxiv - Cell Biology 2022Quote: ... and proteins were transferred to a nitrocellulose membrane (Merck Millipore). The membrane was then incubated with 5% BSA in Tris-buffered saline with tween 20 (Santa Cruz Biotechnology ...
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bioRxiv - Systems Biology 2020Quote: ... proteins were transferred to Immobilon FL PVDF membrane (Merck-Millipore) using semi-dry transfer (25 V for 30 min.) ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred to 0.45 μm PVDF membranes (Merck Millipore), and blocked with 5% skim milk in TBS-0.1% Tween20 (TBST ...
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bioRxiv - Microbiology 2020Quote: ... Protein concentrations were determined with a direct detection spectrometer (Merck), and purified proteins were stored at −80°C ...
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bioRxiv - Microbiology 2021Quote: ... Purified proteins were desalted using a PD-10 column (Merck), concentrated using an Amicon Ultra-4 10 kDa cut-off spin column (Merck) ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-myelin basic protein (MBP) (Polyclonal, Merck, Darmstadt, Germany); mouse anti-neurofilament-H protein (clone NE14 ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-neurofilament-H protein (clone NE14; Merck, Darmstadt, Germany); mouse anti-dephosphorylated neurofilament protein (clone smi32 ...
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bioRxiv - Neuroscience 2020Quote: ... Protein was transferred to Immobilon-FL PVDF membrane (Merck, USA) using a wet transfer system (Bio-Rad ...
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bioRxiv - Biochemistry 2020Quote: ... Equal amounts of protein (as determined by Merck Direct Detect) were then separated by 4 to 12% bis-tris SDS-PAGE in NuPAGE MOPS SDS Running Buffer (Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... mouse anti-neurofilament-H protein (clone NE14; Merck, Darmstadt, Germany); rabbit anti-IBA1 (Polyclonal IgG ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit anti-myelin basic protein (MBP) (Polyclonal, Merck, Darmstadt, Germany); mouse anti-neurofilament-H protein (clone NE14 ...
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bioRxiv - Neuroscience 2020Quote: ... Total protein was quantified using Ponceau S staining (Merck, UK) and membranes were blotted using antibodies raised against murine haem oxygenase-1 (HO-1 ...
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bioRxiv - Systems Biology 2020Quote: ... Separated proteins were transferred onto PVDF membranes (Merck Millipore, IPVH00010). Membranes were blocked in 5% BSA in PBST and antibodies diluted in 2% BSA in PBST ...
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bioRxiv - Systems Biology 2020Quote: ... Proteins were precipitated using five volumes of chilled acetone (Merck) at −20°C overnight ...
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bioRxiv - Plant Biology 2021Quote: ... The protein extracts were then filtered through Miracloth (Merck, Germany) and subjected to a Co-IP analysis.
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bioRxiv - Plant Biology 2020Quote: ... the proteins were electroblotted to a PVDF membrane (Merck Millipore) in the following buffer ...
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bioRxiv - Microbiology 2021Quote: ... The proteins were next transferred onto Immobilon P membrane (Merck) and probed with Ni-NTA-HRP.
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bioRxiv - Cell Biology 2020Quote: ... Proteins were electroblotted to 0.45μm PVDF membranes (Imobilon-P, Merck Millipore ...
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bioRxiv - Cell Biology 2021Quote: ... and proteins were transferred to PVDF membranes (Immobilon-FL, Merck). For dot blots ...
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bioRxiv - Immunology 2022Quote: ... Protein concentrations of lysates were determined using BCA assay (Merck). Proteins in culture supernatants were precipitated using methanol/chloroform ...
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bioRxiv - Cell Biology 2022Quote: ... The separated proteins were transferred onto PVDF membranes (Merck, Germany), blocked with 5% non-fat powdered milk in 0.02% Tween 20 in TBS (TBST ...
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bioRxiv - Immunology 2022Quote: ... followed by protein A purification (ProSep® Ultra Plus; Merck). Elution of hcAbs was achieved by a pH shift with glycine buffer as described previously [13] ...
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bioRxiv - Cancer Biology 2022Quote: ... Purified proteins were dialyzed against 20 mm Tris/HCl (MERCK, Calbiochem ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein samples were concentrated using 50K centrifugal filters (Amicon, Merck) and stored at -80 °C.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the mouse anti-ubiquitinated proteins 1:1000 (04-263, Merck), the rabbit anti-LC3B 1:1000 (NB600-1384 ...
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bioRxiv - Cell Biology 2022Quote: ... Separated proteins were transferred onto Immobilon-P PVDF membrane (Merck) using Trans-Blot SD Semi-Dry Electrophoretic Transfer Cell (Bio-Rad Laboratories) ...
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bioRxiv - Systems Biology 2019Quote: ... Protein concentration was measured using DirectDetect® infrared spectrometer (Merck). A 10 μg aliquot of 1 mg/mL protein extract was denatured by heating at 95°C for 5 minutes ...
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bioRxiv - Plant Biology 2019Quote: ... After transferring proteins from gels to PVDF membranes (Merck-Millipore) using Trans-Blot Turbo System (Bio-Rad) ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein G beads and Taq polymerase were purchased from Merck-Millipore (USA) ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein pellets were washed five times with cold Acetone (Merck) and submitted for subsequent mass spectrometric analyses by the Functional Genomics Center Zurich (FGCZ) ...
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were transferred to a PVDF membrane (ISEQ00010, Merck Millipore) blocked and blotted using the iBind system (SLF1020 ...
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bioRxiv - Biochemistry 2020Quote: ... Protein was eluted and concentrated (Amicon Centrifugal Filter Units, Merck) followed by size exclusion chromatography (Superdex 75 16/60 ...
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bioRxiv - Biochemistry 2020Quote: ... Protein was eluted and concentrated (Amicon Centrifugal Filter Units, Merck) followed by size exclusion chromatography (Superdex 75 16/60 ...
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bioRxiv - Biochemistry 2020Quote: ... Protein was eluted and concentrated (Amicon Centrifugal Filter Units, Merck) followed by size exclusion chromatography (Superdex 75 16/60 ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were transferred onto 0.2 μm pore PVDF membranes (Merck). Nonspecific background was blocked in 5% w/v dry skimmed milk (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Proteins were transferred onto a polyvinylidene fluoride membrane (Merck Millipore), followed by blocking in Tris-buffered saline containing 0.1% Tween-20 (TBST ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred to a PVDF membrane (IPFL00010, Merck Millipore) and blocked with 5% skimmed milk- PBST ...
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bioRxiv - Cancer Biology 2022Quote: ... the proteins were transferred on a nitrocellulose membrane (Amersham, Merck,) which was probed over-night at 4°C with specific antibodies diluted in 1-3% skimmed milk-PBS-0.1% Tween solution ...
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bioRxiv - Cell Biology 2022Quote: ... 30 μl Protein G Sepharose®(#GE17-0618-01, Merck) was coupled to 1 μg monoclonal mouse anti-GFP antibody (#11814460001 ...
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bioRxiv - Molecular Biology 2023Quote: ... After the transfer of proteins onto a PVDF membrane (Merck), blocking was carried out (5% skimmed milk in PBS) ...