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Citations for Merck :
51 - 100 of 1248 citations for Recombinant Red Fluorescent Protein His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Recombinant BG4 (recBG4) (Millipore Merck) was added as control.
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bioRxiv - Biochemistry 2020Quote: ... The recombinant protein was dialyzed overnight using an Amicon Ultra 15 mL spin dialysis column 10 000 MWCO (Merck, Germany). Purified protein was quantified using Bradford’s assay ...
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bioRxiv - Neuroscience 2023Quote: ... the affinity resin was prepared by incubating 100 µg of recombinant protein with 20 µl of nickel beads (PureProteome Nickel magnetic beads, Merck) in 50 mM Pull-Down Buffer PDB (Tris–HCl pH 7.4 ...
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bioRxiv - Biochemistry 2024Quote: ... Affinity-purified recombinant TrK13 proteins (WT, C580Y, R539T and C580Y) or commercially purchased BSA (A7030) or lysozyme (L6876) (Merck, Germany), each at 10 µM concentration ...
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bioRxiv - Plant Biology 2021Quote: ... and Nile Red (Merck) as described previously (Luginbuehl et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... Total protein content in each eluate was measured using infra-red spectroscopy (Direct Detect, Merck Millipore, Burlington, MA, USA). The remainder was aliquoted and stored at −80 °C.
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bioRxiv - Plant Biology 2020Quote: ... The recombinant MBP fusion protein was concentrated with an Amicon Ultra-15 centrifugal filter unit (Ultracel 3K; Merck Millipore, Darmstadt, Germany) and used directly for electrophoretic mobility shift assay (EMSA).
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bioRxiv - Physiology 2020Quote: ... tau/NTF or N-terminal (243-441) tau/CTF cDNA was used to produce recombinant tau protein in Escherichia coli BL21 (DE3) (Merck Millipore). Purification was performed as described previously (Hasegawa et al. ...
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bioRxiv - Neuroscience 2020Quote: ... The proteins were transferred to a low fluorescent polyvinylidene difluoride membrane Immobilon-FL (0.45 μm pore size; Merck-Millipore, Czech Republic) by a Mini Trans-Blot® cell system (Bio-Rad ...
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bioRxiv - Developmental Biology 2023Quote: ... they were stained with Carmine red solution (5 mg Carmine Red (Merck® – C1022), 5 ml 37% HCL ...
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bioRxiv - Neuroscience 2023Quote: ... 0.5% Phenol Red Solution (Merck), 0.2% NaHCO3 (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2020Quote: ... Fractions containing the respective recombinant proteins were pooled and concentrated using Amicon® Ultra-15 spin concentrators with 10 kDa cut-off (Merck Millipore). Concentrated protein pools were run on a Superdex 200 Increase 10/300 GL ...
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bioRxiv - Physiology 2020Quote: ... for 45 s at 80 °C and alizarin red (Alizarin red S staining solution; Merck) for 1.5 min ...
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bioRxiv - Biochemistry 2024Quote: ... we utilized the His-Bind purification kit (Merck, USA) followed by manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... PDGCLs were lentivirally transduced with the MISSION® shRNA vector pLKO.1-puro-CMV-Turbo green fluorescent protein (TurboGFP)_shnon-target (#SHC016, Sigma, part of Merck, Darmstadt, Germany) for cytosolic TurboGFP expression ...
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bioRxiv - Cell Biology 2021Quote: ... human recombinant insulin (100 µg/mL; Merck), recombinant human insulin-like growth factor 1 (hIGF1 ...
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bioRxiv - Molecular Biology 2020Quote: ... Recombinant human TNFα (GF023) was from Merck. Purified anti-His tag mouse antibody was from BioLegend (USA) ...
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bioRxiv - Bioengineering 2024Quote: ... Red blood cells were lysed with red blood cell lysis buffer (Merck, Fontenay-Sous-Bois, France). Next ...
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bioRxiv - Biophysics 2022Quote: ... Nile red was procured from Merck-Sigma Aldrich (India) ...
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bioRxiv - Pathology 2023Quote: ... Oil Red O (Sigma-Merck, Germany) dye was used for staining lipids ...
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bioRxiv - Neuroscience 2023Quote: ... TMR red (Merck, cat. no 12156792910) in combination TH staining ...
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bioRxiv - Pathology 2022Quote: ... recombinant anti-Cre (Merck Millipore, 69050, 1:1000) and revealed using Histofine reagent (Nichirei Biosciences) ...
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bioRxiv - Neuroscience 2022Quote: ... and coverslipped with fluorescent mounting medium (Merck Millipore). Staining specificity was determined by omission of primary antibodies ...
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bioRxiv - Cancer Biology 2022Quote: ... incubated with Red Blood Lysis buffer (Merck) and transferred through a 40 μm cell strainer ...
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bioRxiv - Cancer Biology 2023Quote: ... and red blood cell lysis buffer (Merck) was subsequently added to the pellet ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.001% phenol red (Merck, 143-74-8), pH 7.8) ...
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bioRxiv - Biophysics 2021Quote: ... anti-6×His and anti-FLAG M2 (Sigma-Aldrich, Merck, USA). The anti-mouse IgG ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was added to a His Trap HP column (Merck) previously equilibrated with buffer A ...
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bioRxiv - Cell Biology 2021Quote: ... previously coupled to 1 ml HI-TRAP NHS columns (GE/Merck) packed with Sepharose pre-activated for covalent coupling of ligands containing primary amino groups ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS; Merck). Immortalized human C20 microglia were kindly provided by David Alvarez-Carbonell (21) ...
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bioRxiv - Plant Biology 2022Quote: ... loaded onto His-Bind® Resin columns (EMD Millipore; Merck KGaA), and the recombinant ZmEREB57 protein was eluted with elution buffer (0.5 M NaCl ...
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bioRxiv - Neuroscience 2022Quote: ... The digested tissue was passed through a 40μm nylon cell strainer (Falcon) and red blood cells were lysed (Red Blood Cells Lysis buffer, Merck). After blocking with mouse FcR Blocking Reagent (MACS Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2021Quote: ... and 2 μg/ml of recombinant TNC (Merck Millipore) added to the medium ...
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bioRxiv - Immunology 2021Quote: ... recombinant hepatitis B surface antigen (HBsAg) manufactured by Merck & Co. ...
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bioRxiv - Biophysics 2020Quote: ... Recombinant DNA reagents and primers were purchased from Merck. As calibration samples in ALEX as well as anisotropy experiments ...
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bioRxiv - Developmental Biology 2022Quote: ... 10 mg/mL Human recombinant Insulin (Merck, Cat. #91077C) and 1% Pen/Strep ...
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bioRxiv - Neuroscience 2023Quote: ... Tau ladder (six human tau recombinant isoforms, Sigma-Merck) was used to identify tau isoforms in NCI-H716 cells ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.1 IU/ml recombinant human FSH (Merck & Co., Inc), 0.125 mg/ml recombinant human hyaluronan (Novozymes) ...
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bioRxiv - Biochemistry 2023Quote: ... Active recombinant LKB1/STRADα/MO25α was purchased from Merck. Gateway pENTR plasmids encoding full length human BRSK1 & BRSK2 were generated as part of the NIH common fund initiative to Illuminate the Druggable Genome (IDG ...
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bioRxiv - Neuroscience 2023Quote: ... The fluorescent dye dihydroethidium (DHE) (Sigma-Aldrich; Merck KGaA) was used to detect the intracellular ROS levels ...
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bioRxiv - Evolutionary Biology 2022Quote: ... individuals were placed in 0.008% alizarin red (alizarin red S monohydrate, Waldeck Chemie, Münster, Germany) and 1% potassium hydroxide (EMSURE, Merck, Darmstadt, Germany) for 24 h ...
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bioRxiv - Cell Biology 2020Quote: ... and Nile red (N3013-100MG, Merck, Darmstadt, Germany) were added to a final concentration of 0.4 μg/mL (1 mM ...
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bioRxiv - Neuroscience 2022Quote: The Duolink In Situ Red system (92101, Merck) was used to detect Stg-Tau interaction in vivo ...
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bioRxiv - Cancer Biology 2023Quote: ... and Duolink Detection Reagents Red or Green (Merck) were used to perform the PLA reaction ...
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bioRxiv - Cell Biology 2023Quote: ... or EZview Red Anti-HA Affinity Gel (Merck), 25 μl of slurry per sample ...
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bioRxiv - Immunology 2024Quote: ... Red blood cell lysis was performed (Merck, 11814389001). Whole transcriptome analyses of FACS sorted CD45+ cells were performed using the BD Rhapsody Single Cell Analysis System (BD ...
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bioRxiv - Microbiology 2021Quote: ... and the supernatant was incubated with His-binding resin (Merck 69670-5) for 10-30 min at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... the supernatant was loaded onto a His-bind resin column (Merck Millipore) (1 ml ...
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bioRxiv - Plant Biology 2023Quote: ... and purified with Ni-NTA His•Bind® Resin agarose (Merck, 70666). The binding reaction was performed in 20 µL of binding buffer [10 mM Tris (pH 8.0) ...
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bioRxiv - Molecular Biology 2021Quote: ... 6xHis-MPB-tagged 43GTPBP6 was expressed in E.coli BL21 (DE3) RIL cells (Merck Millipore) grown in LB media ...