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Citations for Merck :
1 - 50 of 277 citations for Recombinant Rat Havcr1 Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... 6×His-tagged recombinant MSP1E3D1 (Sigma-Aldrich, Merck, USA) and FLAG-tagged recombinant mCardinal (kindly provided by Jakub Czapiński ...
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bioRxiv - Immunology 2023Quote: ... The eluted His-Myc tagged recombinants were further concentrated using 3kDa ultra concentration units (Merck, Germany). The GST-tagged recombinant proteins were affinity purified using Glutathione Sepharose 4B beads and purified in batch mode ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were purified with His-Bind Resin and His-Bind Buffers (Merck Millipore) according to manufacturer’s protocol and stored at a concentration of 2mg/ml in elution buffer (Merck Millipore ...
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bioRxiv - Cell Biology 2020Quote: ... His-tagged proteins were immobilized on Ni-NTA His-Bind® Superflow™ Resin (Merck Millipore, 70691), washed and eluted in SB buffer (30 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... His tagged proteins were labeled using Ni-NTA-ATTO 488 (Merck Group) at a ratio of ATTO/protein = 1/500 in MN buffer (50 mM MES pH 6 ...
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bioRxiv - Plant Biology 2020Quote: ... His-tagged proteins were washed and purified using the Amicon Pro System (Merck) according to manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: His-tagged proteins were labeled using Ni-NTA-ATTO 550 or Ni-NTA-ATTO 647 from Merck Group ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 ug inactive MEK1 (C-terminally His-tagged) in a total volume of 40 μL buffer (20 mM Hepes pH 8 (MERCK, Sigma Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... The His6-tagged proteins were purified using Immobilised Metal Affinity Chromatography (IMAC) with Ni-NTA His•Bind® Resin (Merck). Fractions containing the protein of interest were then dialysed against low-salt (50mM Tris pH8.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The His6-tagged proteins were purified using Immobilised Metal Affinity Chromatography (IMAC) with Ni-NTA His•Bind® Resin (Merck). Fractions containing the protein of interest were then dialysed against low-salt buffer (50mM Tris pH7.0 ...
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bioRxiv - Biochemistry 2023Quote: ... His-tagged Trx1 was purified on a Ni2+ sepharose resin following the manufactureŕs instructions (cOmplete™ His-Tag Purification Resin, Merck).
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bioRxiv - Zoology 2020Quote: ... recombinant RhATG5 and RhATG5191-199Δ were affinity-purified using Ni-NTA His•Bind Resin (Merck-Millipore, Darmstadt, Germany). Recombinant RhATG5 and RhATG5191-199Δ were then incubated with 10µg μ-calpain (Merck ...
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bioRxiv - Biochemistry 2022Quote: ... The His6- tagged proteins were purified using Immobilised Metal Affinity Chromatography (IMAC) with 2mL Ni-NTA His•Bind® Resin (Merck), followed by incubation with TEV protease for 12 hours at 4 °C to remove the His6 tag ...
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bioRxiv - Cell Biology 2023Quote: ... 2020) containing 300 ng GST-tagged MP-GAP fragments and 200 ng recombinant human Aurora A kinase (Merck, 14-511), 1mCi of [gamma-P32]ATP (Hartmann Analytic ...
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bioRxiv - Neuroscience 2019Quote: ... the rats were treated with either 170,000 IU/kg IntronA (human recombinant interferon-alpha 2b, Merck Sharp & Dohme Limited), or saline (0.9% NaCl ...
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bioRxiv - Molecular Biology 2022Quote: ... or anti-His (Merck) for His-G5845–1508) ...
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bioRxiv - Immunology 2019Quote: ... Fc IgG (AG714, Merck Millipore) at 2 μg/ml in 150 μl of PBS for one hour ...
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bioRxiv - Biophysics 2019Quote: ... supplemented with 10 % FCS (Merck), 100 µg ml−1 penicillin (Life technologies) ...
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bioRxiv - Biophysics 2019Quote: ... supplemented with 10% FCS (Merck), 100 µg ml−1 penicillin (Life technologies) ...
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bioRxiv - Biophysics 2020Quote: ... supplemented with 10% FCS (MERCK), 100 μg ml−1 penicillin (Life technologies) ...
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bioRxiv - Biochemistry 2021Quote: ... supplemented with 10% FCS (Merck) and 2 mM L-glutamine (Thermo Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... + 10% FCS (Merck Millipore S0615) + 1% P/S (Thermo Fisher #15140122) ...
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bioRxiv - Immunology 2024Quote: ... supplemented with 10% FCS (Merck), 2mM L-Glutamine (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: ... Immunoprecipitation of cell lysates overexpressing FLAG-tagged or GST-tagged proteins were performed using anti-FLAG-M2 affinity beads (Merck) or glutathione-Sepharose beads (Cytiva).
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bioRxiv - Immunology 2020Quote: ... 350 ng/mL active ERK2-GST tagged (Merck) or 175 ng/mL ERK1 plus 175 ng/mL ERK2-GST for 30 min at 30°C.
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% HI-FBS (Merck/Biochrom), 2 mM L-glutamine (Merck/Biochrom) ...
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bioRxiv - Systems Biology 2023Quote: ... Primary his tag antibody (05-949, Merck), stored in a skim milk solution ...
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bioRxiv - Molecular Biology 2024Quote: ... or pET30c (HIS) (Merck/Novagen, Darmstadt, Germany) using EcoRI and NotI sites ...
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bioRxiv - Cell Biology 2022Quote: ... Recombinant BG4 (recBG4) (Millipore Merck) was added as control.
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bioRxiv - Cell Biology 2020Quote: ... containing 10% FCS (Merck KGA, Germany), 4 mM glutamine (Merck KGA ...
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bioRxiv - Immunology 2020Quote: ... and 10% Fetal calf serum (FCS) (Merck). Both cell lines were cultivated in T75 culture flasks ...
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bioRxiv - Biochemistry 2024Quote: ... we utilized the His-Bind purification kit (Merck, USA) followed by manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... human recombinant insulin (100 µg/mL; Merck), recombinant human insulin-like growth factor 1 (hIGF1 ...
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bioRxiv - Molecular Biology 2020Quote: ... Recombinant human TNFα (GF023) was from Merck. Purified anti-His tag mouse antibody was from BioLegend (USA) ...
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bioRxiv - Cell Biology 2021Quote: ... Myc-tagged proteins were immuno-precipitated with the 9E10 antibody (Merck); Rpb1 was immunoprecipitated using the 8WG16 antibody (Merck).
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bioRxiv - Cell Biology 2021Quote: ... anti-HA (rat; Merck; 11867423001), anti-exportin-1 (mouse ...
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bioRxiv - Immunology 2021Quote: ... A rat IgG1 antibody (Merck) was used as the isotype-matched control ...
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bioRxiv - Molecular Biology 2024Quote: ... Rat monoclonal (1:250; Merck) and incubated in 4°C for 5 nights ...
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bioRxiv - Biophysics 2019Quote: ... and 10 ml RPMI containing 0.75 % FCS (Merck) in a common 50-ml Eppendorf tube and heated up to 68°C using a common water bath ...
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bioRxiv - Biochemistry 2021Quote: ... Fluorinated oil (FC- 40, Merck, Kenilworth, NJ, USA) was used to remove the excess amounts of the reaction solution and to seal the reactor (Supplementary Fig ...
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bioRxiv - Bioengineering 2023Quote: ... FCS was mixed with 50% PEG-10,000 (Merck) at a 5:1 ratio and rotated at 4 °C for 3 h ...
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bioRxiv - Cell Biology 2023Quote: ... apically coated with 0,1 % collagen from rat tail (collagen type I, rat tail; Merck, Germany) at a density of 1.5 × 105 cells / well and incubated at 37°C with 5% CO2 ...
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bioRxiv - Pathology 2022Quote: ... recombinant anti-Cre (Merck Millipore, 69050, 1:1000) and revealed using Histofine reagent (Nichirei Biosciences) ...
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bioRxiv - Cell Biology 2023Quote: ... Recombinant active DAPK3 protein was obtained from Merck.
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bioRxiv - Genomics 2020Quote: ... supplemented with 15% fetal calf serum (FCS) (Merck Millipore), 0.1mM b-mercaptoethanol (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2022Quote: ... was supplemented with 10% fetal calf serum (FCS) (Merck), 1x non-essential amino acids (NEAA ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... supplemented with 10% foetal calf serum (FCS, Merck, Germany). Once confluent ...
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bioRxiv - Bioengineering 2022Quote: ... supplemented with 10% Fetal Calf Serum (FCS, #S0615, Merck), 4 mM L-glutamine (#25030-024 ...
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bioRxiv - Cell Biology 2023Quote: ... rat anti CRB3 1E6 (MABT1366 Merck), mouse anti-paxillin (BD transduction 612405) ...
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bioRxiv - Biophysics 2021Quote: ... anti-6×His and anti-FLAG M2 (Sigma-Aldrich, Merck, USA). The anti-mouse IgG ...