Labshake search
Citations for Merck :
1 - 50 of 2345 citations for Recombinant Mouse Melanoma Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2021Quote: ... 6×His-tagged recombinant MSP1E3D1 (Sigma-Aldrich, Merck, USA) and FLAG-tagged recombinant mCardinal (kindly provided by Jakub Czapiński ...
-
bioRxiv - Immunology 2023Quote: ... The eluted His-Myc tagged recombinants were further concentrated using 3kDa ultra concentration units (Merck, Germany). The GST-tagged recombinant proteins were affinity purified using Glutathione Sepharose 4B beads and purified in batch mode ...
-
bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were purified with His-Bind Resin and His-Bind Buffers (Merck Millipore) according to manufacturer’s protocol and stored at a concentration of 2mg/ml in elution buffer (Merck Millipore ...
-
bioRxiv - Cell Biology 2020Quote: ... His-tagged proteins were immobilized on Ni-NTA His-Bind® Superflow™ Resin (Merck Millipore, 70691), washed and eluted in SB buffer (30 mM HEPES-NaOH pH 7.5 ...
-
bioRxiv - Biochemistry 2023Quote: ... His tagged proteins were labeled using Ni-NTA-ATTO 488 (Merck Group) at a ratio of ATTO/protein = 1/500 in MN buffer (50 mM MES pH 6 ...
-
bioRxiv - Plant Biology 2020Quote: ... His-tagged proteins were washed and purified using the Amicon Pro System (Merck) according to manufacturer’s instructions.
-
bioRxiv - Cancer Biology 2023Quote: ECM cell adhesion assay was performed as per manufacturer’s instructions (Merck). Briefly ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were treated with recombinant mouse TNFα (10 ng/ml, Merck 654245).
-
bioRxiv - Biochemistry 2023Quote: His-tagged proteins were labeled using Ni-NTA-ATTO 550 or Ni-NTA-ATTO 647 from Merck Group ...
-
bioRxiv - Cancer Biology 2020Quote: ... For generation of conditioned melanoma media for immune cell inhibition experiments tryptophan (Merck, Darmstadt, Germany) was added to the culture media to a final concentration of 200 µM to allow efficient kynurenine production in response to IDO1 expression.
-
bioRxiv - Cancer Biology 2022Quote: ... 1 ug inactive MEK1 (C-terminally His-tagged) in a total volume of 40 μL buffer (20 mM Hepes pH 8 (MERCK, Sigma Aldrich ...
-
bioRxiv - Biochemistry 2022Quote: ... The His6-tagged proteins were purified using Immobilised Metal Affinity Chromatography (IMAC) with Ni-NTA His•Bind® Resin (Merck). Fractions containing the protein of interest were then dialysed against low-salt (50mM Tris pH8.5 ...
-
bioRxiv - Biochemistry 2022Quote: ... The His6-tagged proteins were purified using Immobilised Metal Affinity Chromatography (IMAC) with Ni-NTA His•Bind® Resin (Merck). Fractions containing the protein of interest were then dialysed against low-salt buffer (50mM Tris pH7.0 ...
-
bioRxiv - Biochemistry 2023Quote: ... His-tagged Trx1 was purified on a Ni2+ sepharose resin following the manufactureŕs instructions (cOmplete™ His-Tag Purification Resin, Merck).
-
bioRxiv - Zoology 2020Quote: ... recombinant RhATG5 and RhATG5191-199Δ were affinity-purified using Ni-NTA His•Bind Resin (Merck-Millipore, Darmstadt, Germany). Recombinant RhATG5 and RhATG5191-199Δ were then incubated with 10µg μ-calpain (Merck ...
-
bioRxiv - Biochemistry 2022Quote: ... The His6- tagged proteins were purified using Immobilised Metal Affinity Chromatography (IMAC) with 2mL Ni-NTA His•Bind® Resin (Merck), followed by incubation with TEV protease for 12 hours at 4 °C to remove the His6 tag ...
-
bioRxiv - Cell Biology 2022Quote: ... the following synthetic lipid molecules (Merck) were added to the reaction ...
-
bioRxiv - Molecular Biology 2024Quote: ... FLAG-tagged proteins were detected using M2 anti-FLAG antibody (mouse, F1084, Merck) followed and fluorescent Anti-Mouse Alexa Fluor™ Plus 800 ...
-
bioRxiv - Cell Biology 2023Quote: ... 2020) containing 300 ng GST-tagged MP-GAP fragments and 200 ng recombinant human Aurora A kinase (Merck, 14-511), 1mCi of [gamma-P32]ATP (Hartmann Analytic ...
-
bioRxiv - Genomics 2019Quote: ... and 100 Units/ml of Recombinant mouse LIF Protein (Merck ESG1107). Culture were seeded at an average density of ∼ 3.8*104 cells/cm2 and passaged every 48 h.
-
bioRxiv - Genomics 2020Quote: ... ESGRO recombinant mouse Leukaemia Inhibitory Factor (LIF) was obtained from Merck Millipore ...
-
bioRxiv - Systems Biology 2020Quote: RPE adhesion medium: DMEM/F12 (D8437, Merck & Co., Inc., NJ, USA), 10% FBS (12007C ...
-
bioRxiv - Cancer Biology 2023Quote: ... Immunoprecipitation of cell lysates overexpressing FLAG-tagged or GST-tagged proteins were performed using anti-FLAG-M2 affinity beads (Merck) or glutathione-Sepharose beads (Cytiva).
-
bioRxiv - Molecular Biology 2022Quote: ... or anti-His (Merck) for His-G5845–1508) ...
-
bioRxiv - Molecular Biology 2021Quote: ... 6xHis-MPB-tagged 43GTPBP6 was expressed in E.coli BL21 (DE3) RIL cells (Merck Millipore) grown in LB media ...
-
bioRxiv - Biochemistry 2020Quote: ... Cell lysates were supplemented with 500 U Recombinant DNAse (Merck 4716728001) and 100 mM PMSF ...
-
bioRxiv - Immunology 2021Quote: ... neutrophils were pre-incubated with 20 μM focal adhesion kinase (FAK) inhibitor-1 (Merck, Darmstadt, Germany), 1 μM Src inhibitor-1 (Merck) ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... These molecules were dissolved in DMSO (cat n° D2438, Sigma-Aldrich Merck). As a control experiment ...
-
bioRxiv - Cell Biology 2020Quote: ... The following small molecules were commercially available: nocodazole (Merck; working concentration: 33 µM), brefeldin A (Epicenter Technologies ...
-
bioRxiv - Biophysics 2019Quote: ... Unreacted Cy5-maleimide molecules were removed using an Amicon ultra centrifugal filter (Merck).
-
bioRxiv - Cell Biology 2021Quote: ... or anti-rabbit IgG (whole molecule)–peroxidase antibody produced in goat (MERCK, A9169) were used as secondary antibodies ...
-
bioRxiv - Immunology 2022Quote: ... MHC class II molecules were purified from supernatants with Chelating Sepharose FF (Merck). Peptide loading was performed in citrate saline buffer (100 mM citrate ...
-
bioRxiv - Developmental Biology 2023Quote: ... Sections with a thickness of 10 µm were cut and placed on EprediaTM SuperFrost PlusTM Adhesion slides (Merck). Slides were stored at −80°C until staining ...
-
bioRxiv - Immunology 2020Quote: ... 350 ng/mL active ERK2-GST tagged (Merck) or 175 ng/mL ERK1 plus 175 ng/mL ERK2-GST for 30 min at 30°C.
-
bioRxiv - Microbiology 2021Quote: ... The binding of CoVHH1-FLAG to S1 subunits-His tag was detected by incubating for 1 hour at room temperature with mouse monoclonal ANTI-FLAG® M2-Peroxidase (Merck) diluted with PBST ...
-
bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% HI-FBS (Merck/Biochrom), 2 mM L-glutamine (Merck/Biochrom) ...
-
bioRxiv - Systems Biology 2023Quote: ... Primary his tag antibody (05-949, Merck), stored in a skim milk solution ...
-
bioRxiv - Molecular Biology 2024Quote: ... or pET30c (HIS) (Merck/Novagen, Darmstadt, Germany) using EcoRI and NotI sites ...
-
bioRxiv - Plant Biology 2020Quote: ... Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat (A0545; Merck-Sigma-Aldrich) was used as secondary antibody following the use of above antibody ...
-
bioRxiv - Plant Biology 2020Quote: ... Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat (A0545; Merck-Sigma-Aldrich) was used as secondary antibody ...
-
bioRxiv - Immunology 2019Quote: ... HLA molecules and peptides were eluted with 1% trifluoroacetic acid (TFA, Merck, Darmstadt, Switzerland) directly into Sep-Pak tC18 100 mg Sorbent 96-well plates (Waters ...
-
bioRxiv - Plant Biology 2019Quote: ... Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat (A0545; Merck-Sigma-Aldrich) was used as secondary antibody following the use of Phospho-p44/42 MAPK antibody.
-
bioRxiv - Cell Biology 2022Quote: ... Recombinant BG4 (recBG4) (Millipore Merck) was added as control.
-
Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: ... plasmid coding for 6x-His-Cas9 were transformed in Rosetta DE3 Novagen competent cells (Merck Millipore, USA). Protein production was induced using autoinduction medium (Formedium ...
-
Contractile acto-myosin network on nuclear envelope remnants positions human chromosomes for mitosisbioRxiv - Cell Biology 2019Quote: Small molecule inhibitors were used with the following concentrations: 1NM-PP1 1µM (Merck Millipore 529581), RO-3306 10µM (Merck Millipore 217699) ...
-
bioRxiv - Developmental Biology 2023Quote: ... medium was changed to N2B27 supplemented with growth factors and small molecule inhibitors: CHIR99201 (Merck), murine BMP4 (PeproTech 315-27) ...
-
bioRxiv - Microbiology 2022Quote: ... Recombinant proteins were obtained after expression at 22°C in B834 (DE3) cells (Merck, Darmstadt, Germany), upon induction with 0.6□mM IPTG at 0.8 OD600 ...
-
bioRxiv - Cell Biology 2021Quote: ... The following small molecule inhibitors were used at the indicated concentration: nocodazole (5 µM; Merck Millipore), CK-666 (100 µM ...
-
bioRxiv - Microbiology 2020Quote: ... circular DNA molecules were amplified with random examers and TempliPhi DNA polymerase (Merck KGaA, Darmstadt, Germany) following manufacturer’s instruction ...
-
bioRxiv - Cancer Biology 2023Quote: Lysates from HEK293 cells overexpressing FLAG-tagged wild typeTP53INP2 or the TP53INP2-3A were affinity-purified with anti-FLAG-M2 affinity beads (Merck). Then ...