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Citations for Merck :
1 - 50 of 774 citations for Recombinant Mouse Fcgr1 His & AVI Tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... 6×His-tagged recombinant MSP1E3D1 (Sigma-Aldrich, Merck, USA) and FLAG-tagged recombinant mCardinal (kindly provided by Jakub Czapiński ...
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bioRxiv - Immunology 2023Quote: ... The eluted His-Myc tagged recombinants were further concentrated using 3kDa ultra concentration units (Merck, Germany). The GST-tagged recombinant proteins were affinity purified using Glutathione Sepharose 4B beads and purified in batch mode ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were purified with His-Bind Resin and His-Bind Buffers (Merck Millipore) according to manufacturer’s protocol and stored at a concentration of 2mg/ml in elution buffer (Merck Millipore ...
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bioRxiv - Cell Biology 2020Quote: ... His-tagged proteins were immobilized on Ni-NTA His-Bind® Superflow™ Resin (Merck Millipore, 70691), washed and eluted in SB buffer (30 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... His tagged proteins were labeled using Ni-NTA-ATTO 488 (Merck Group) at a ratio of ATTO/protein = 1/500 in MN buffer (50 mM MES pH 6 ...
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bioRxiv - Plant Biology 2020Quote: ... His-tagged proteins were washed and purified using the Amicon Pro System (Merck) according to manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: His-tagged proteins were labeled using Ni-NTA-ATTO 550 or Ni-NTA-ATTO 647 from Merck Group ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 ug inactive MEK1 (C-terminally His-tagged) in a total volume of 40 μL buffer (20 mM Hepes pH 8 (MERCK, Sigma Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... The His6-tagged proteins were purified using Immobilised Metal Affinity Chromatography (IMAC) with Ni-NTA His•Bind® Resin (Merck). Fractions containing the protein of interest were then dialysed against low-salt (50mM Tris pH8.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The His6-tagged proteins were purified using Immobilised Metal Affinity Chromatography (IMAC) with Ni-NTA His•Bind® Resin (Merck). Fractions containing the protein of interest were then dialysed against low-salt buffer (50mM Tris pH7.0 ...
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bioRxiv - Biochemistry 2023Quote: ... His-tagged Trx1 was purified on a Ni2+ sepharose resin following the manufactureŕs instructions (cOmplete™ His-Tag Purification Resin, Merck).
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bioRxiv - Zoology 2020Quote: ... recombinant RhATG5 and RhATG5191-199Δ were affinity-purified using Ni-NTA His•Bind Resin (Merck-Millipore, Darmstadt, Germany). Recombinant RhATG5 and RhATG5191-199Δ were then incubated with 10µg μ-calpain (Merck ...
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bioRxiv - Biochemistry 2022Quote: ... The His6- tagged proteins were purified using Immobilised Metal Affinity Chromatography (IMAC) with 2mL Ni-NTA His•Bind® Resin (Merck), followed by incubation with TEV protease for 12 hours at 4 °C to remove the His6 tag ...
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bioRxiv - Molecular Biology 2024Quote: ... FLAG-tagged proteins were detected using M2 anti-FLAG antibody (mouse, F1084, Merck) followed and fluorescent Anti-Mouse Alexa Fluor™ Plus 800 ...
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bioRxiv - Immunology 2020Quote: ... The elimination of the N-terminal His tag was performed through the cleavage with biotinylated thrombin included in the Thrombin Cleavage Capture Kit (Merck, Millipore) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... 2020) containing 300 ng GST-tagged MP-GAP fragments and 200 ng recombinant human Aurora A kinase (Merck, 14-511), 1mCi of [gamma-P32]ATP (Hartmann Analytic ...
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bioRxiv - Genomics 2019Quote: ... and 100 Units/ml of Recombinant mouse LIF Protein (Merck ESG1107). Culture were seeded at an average density of ∼ 3.8*104 cells/cm2 and passaged every 48 h.
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bioRxiv - Genomics 2020Quote: ... ESGRO recombinant mouse Leukaemia Inhibitory Factor (LIF) was obtained from Merck Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... and a biotinylated donkey antibody to mouse IgG (10 μg/ml; AP192B, Merck Millipore). The sections were further incubated with Alexa594-conjugated streptavidin (2.5 μg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were treated with recombinant mouse TNFα (10 ng/ml, Merck 654245).
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bioRxiv - Molecular Biology 2022Quote: ... or anti-His (Merck) for His-G5845–1508) ...
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bioRxiv - Biophysics 2019Quote: ... fractions containing monomeric PSCFP2*-AVI-3C-12xHis were concentrated using Amicon Ultra-4 centrifugal filters (10 kDa cut-off, Merck) in the presence of 100 µM Tris(2-carboxyethyl)phosphine (TCEP ...
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bioRxiv - Molecular Biology 2019Quote: ... Biotinylated porcine intestinal mucosa heparin (Merck Millipore) was immobilized on streptavidin biosensors at 10 µg/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... Immunoprecipitation of cell lysates overexpressing FLAG-tagged or GST-tagged proteins were performed using anti-FLAG-M2 affinity beads (Merck) or glutathione-Sepharose beads (Cytiva).
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bioRxiv - Immunology 2020Quote: ... 350 ng/mL active ERK2-GST tagged (Merck) or 175 ng/mL ERK1 plus 175 ng/mL ERK2-GST for 30 min at 30°C.
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bioRxiv - Microbiology 2021Quote: ... The binding of CoVHH1-FLAG to S1 subunits-His tag was detected by incubating for 1 hour at room temperature with mouse monoclonal ANTI-FLAG® M2-Peroxidase (Merck) diluted with PBST ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% HI-FBS (Merck/Biochrom), 2 mM L-glutamine (Merck/Biochrom) ...
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bioRxiv - Systems Biology 2023Quote: ... Primary his tag antibody (05-949, Merck), stored in a skim milk solution ...
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bioRxiv - Molecular Biology 2024Quote: ... or pET30c (HIS) (Merck/Novagen, Darmstadt, Germany) using EcoRI and NotI sites ...
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bioRxiv - Cell Biology 2022Quote: ... Recombinant BG4 (recBG4) (Millipore Merck) was added as control.
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bioRxiv - Biochemistry 2024Quote: ... we utilized the His-Bind purification kit (Merck, USA) followed by manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... human recombinant insulin (100 µg/mL; Merck), recombinant human insulin-like growth factor 1 (hIGF1 ...
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bioRxiv - Molecular Biology 2020Quote: ... Recombinant human TNFα (GF023) was from Merck. Purified anti-His tag mouse antibody was from BioLegend (USA) ...
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bioRxiv - Cell Biology 2021Quote: ... Myc-tagged proteins were immuno-precipitated with the 9E10 antibody (Merck); Rpb1 was immunoprecipitated using the 8WG16 antibody (Merck).
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bioRxiv - Neuroscience 2020Quote: ... or 1:500 biotinylated Wisteria floribunda agglutinin WFA (#L8258; Merck, Germany), diluted in the same blocking solution ...
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bioRxiv - Neuroscience 2023Quote: Biotinylated surface proteins were isolated using streptavidin-coated agarose beads (Merck). The beads were washed 2 times in lysis buffer by centrifugation at low speed (<1.5k RCF) ...
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bioRxiv - Pathology 2022Quote: ... recombinant anti-Cre (Merck Millipore, 69050, 1:1000) and revealed using Histofine reagent (Nichirei Biosciences) ...
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bioRxiv - Cell Biology 2023Quote: ... Recombinant active DAPK3 protein was obtained from Merck.
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bioRxiv - Biophysics 2021Quote: ... anti-6×His and anti-FLAG M2 (Sigma-Aldrich, Merck, USA). The anti-mouse IgG ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was added to a His Trap HP column (Merck) previously equilibrated with buffer A ...
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bioRxiv - Cell Biology 2021Quote: ... previously coupled to 1 ml HI-TRAP NHS columns (GE/Merck) packed with Sepharose pre-activated for covalent coupling of ligands containing primary amino groups ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS; Merck). Immortalized human C20 microglia were kindly provided by David Alvarez-Carbonell (21) ...
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bioRxiv - Plant Biology 2022Quote: ... loaded onto His-Bind® Resin columns (EMD Millipore; Merck KGaA), and the recombinant ZmEREB57 protein was eluted with elution buffer (0.5 M NaCl ...
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bioRxiv - Microbiology 2020Quote: ... GST bound protein was then cleaved using biotinylated thrombin (Merck Millipore; 69672) according to the manufacturers instructions overnight at 4°C ...
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bioRxiv - Physiology 2022Quote: ... and biotinylated donkey antibody to rabbit IgG (1:100; AP182B, Merck Millipore) for 1 hr at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 mg/mL (1:500 dilution) of biotinylated anti-phosphotyrosine 4G10 (Merck) antibody and incubated for 1 hour ...
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bioRxiv - Microbiology 2022Quote: ... and the FLAG-tagged proteins were immunoprecipitated with anti-FLAG agarose (A2220, MERCK). Input samples were collected as described above.
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bioRxiv - Cancer Biology 2021Quote: ... and 2 μg/ml of recombinant TNC (Merck Millipore) added to the medium ...
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bioRxiv - Immunology 2021Quote: ... recombinant hepatitis B surface antigen (HBsAg) manufactured by Merck & Co. ...
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bioRxiv - Biophysics 2020Quote: ... Recombinant DNA reagents and primers were purchased from Merck. As calibration samples in ALEX as well as anisotropy experiments ...