Labshake search
Citations for Merck :
501 - 550 of 779 citations for Recombinant Human V erb b2 Erythroblastic Leukemia Viral Oncogene Homolog avian His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... The binding of CoVHH1-FLAG to S1 subunits-His tag was detected by incubating for 1 hour at room temperature with mouse monoclonal ANTI-FLAG® M2-Peroxidase (Merck) diluted with PBST ...
-
bioRxiv - Immunology 2020Quote: ... The elimination of the N-terminal His tag was performed through the cleavage with biotinylated thrombin included in the Thrombin Cleavage Capture Kit (Merck, Millipore) following manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: Recombinant full length SIC proteins were purified using affinity chromatography with a His-bind column as per manufacturer’s instructions (Novagen, Merck, Darmstadt, Germany). sic1.300 ...
-
bioRxiv - Biochemistry 2022Quote: ... The His-tag-free Mpro in the flow-through was concentrated by using Amicon Ultra 15 centrifugal filters (10 kD, Merck Millipore) at 2773 x g ...
-
bioRxiv - Cell Biology 2020Quote: Recombinant protein expression was performed using Eschericia coli strain Rosetta™ 2 (DE3) (Novagen) (Merck Cat. No 71403). Protein expression was always performed using freshly transformed chemically competent E ...
-
bioRxiv - Immunology 2019Quote: ... Cells were interferon stimulated by the addition of 100 IU/ml recombinant interferon-β (IFN-β; Merck, 407318) to the growth media for 24 h ...
-
bioRxiv - Neuroscience 2019Quote: Plasma levels of adiponectin and leptin were measured using commercially available kits (Human Leptin Enzyme Immunoassay, Merck, Cat. #A05174 and Human Adiponectin ELISA, Merck, Cat. # EZHADP-61K). The minimum detectable dose of leptin was 7.8 pg/mL and that of adiponectin was 0.891 μg/mL.
-
bioRxiv - Developmental Biology 2022Quote: ... Heads including both eyes were fixed in 4% w/v paraformaldehyde (PFA, Merck) in 0.1M phosphate buffer overnight at 4°C and the remaining tissue were kept for genotyping by conventional PCR ...
-
bioRxiv - Immunology 2021Quote: ... The pfu were counted following staining with 1 % (w/v) crystal violet (Merck).
-
bioRxiv - Genomics 2020Quote: ... 0.1% (w/v) SDS and wet-transferred to Immobulin-P (Merck Millipore, IPVH00010) or Immobulin-FL membranes (Merck Millipore ...
-
bioRxiv - Neuroscience 2019Quote: ... 500 µL for bulk synaptosomes) in 1% (w/v) sodium deoxycholate (SDC, Merck) in 100 mM Tris pH 8.1 ...
-
bioRxiv - Biochemistry 2020Quote: ... Grids were then stained with 2% (w/v) uranyl acetate pH 4 (Merck) for 1 min ...
-
bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% (w/v) fetal calf serum (FCS) (Merck Millipore, Berlin, Germany) and 1% (w/v ...
-
bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% (w/v) fetal calf serum (FCS) (Merck Millipore, Berlin, Germany) and 1% (w/v ...
-
bioRxiv - Biophysics 2020Quote: ... 100 μl of 25% w/v grade I glutaraldehyde (GA) (Merck, Darmstadt, Germany) was then pipetted on the surface and incubated for 30 min ...
-
bioRxiv - Microbiology 2020Quote: ... and 1.1% (w/v) of a mild detergent component corresponding to digitonin (Merck) and n-dodecyl β-D-maltoside (DDM ...
-
bioRxiv - Biophysics 2020Quote: 100 μl of 0.1% w/v poly-L-lysine (PLL) (Merck, Darmstadt, Germany) solution was pipetted onto a freshly cleaved mica surface (Ted Pella ...
-
bioRxiv - Cell Biology 2021Quote: ... in a 0.01 % (w/v) naphtol AS-MX solution (Sigma Aldrich, Merck, Germany), according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... pH 7.6) containing 10% w/v milk powder (Merck Life Science UK Ltd). Membranes were incubated with primary antibody overnight and then with horseradish peroxidase (HRP)-conjugated secondary antibodies (Agilent Technologies ...
-
bioRxiv - Biophysics 2022Quote: ... and blocking them with 5 w/v% bovine serum albumin (BSA, Merck KGaA), both diluted in PBS for 1 hour ...
-
bioRxiv - Biochemistry 2023Quote: ... The 100-fold dilution of culture was made up to 10-4 dilutions and spotted on TYE plates (Hi-Media Laboratories Ltd., India) having 1% glucose (Merck & Co., USA) and ampicillin (Gold Biotechnology ...
-
bioRxiv - Immunology 2023Quote: ... Lysate was clarified by spinning cells for 10 min at 20,000 × g before loading supernatant onto cOmplete™ His-Tag purification resin (Merck; cat # 5893682001). Bound proteins were washed with 20 mM tris (pH 8.0) ...
-
bioRxiv - Immunology 2021Quote: ... Human pancreatic beta cell line 1.4E7 was purchased from Merck and cultured in RPMI-1640 medium (Thermo Fisher Scientific ...
-
bioRxiv - Bioengineering 2021Quote: ... Part B was made of 10U/mL human thrombin (Merck) in DMEM/F12 ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... and purified human C3a was purchased from Merck (Perth, Australia). Recombinant human C5a (rhC5a ...
-
bioRxiv - Immunology 2020Quote: ... FITC conjugated goat anti-human IgG Fc was from Merck, Dorset ...
-
bioRxiv - Microbiology 2023Quote: ... sensitivity assays against human serum (Merck Millipore, Burlington, MA, USA), colistin (Sigma-Aldrich ...
-
bioRxiv - Bioengineering 2023Quote: ... The human cardiomyocyte cell line AC16 was purchased from Merck Millipore (Burlington ...
-
Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... and HRP-conjugated goat anti-human IgG (AP309P, Merck KGaA). Protein detection was done with enhanced chemiluminescence (ECL ...
-
bioRxiv - Genomics 2023Quote: ... After incubation with FcBlock (PBS + 12% human serum AB (Merck)) for 10 min at 4°C ...
-
bioRxiv - Cell Biology 2024Quote: ... coated with 50 µg/ml human serum fibronectin (Merck, Germany). Imaging was performed on a Nikon Widefield Ti2 equipped with a sCMOS ...
-
bioRxiv - Cell Biology 2020Quote: Recombinant GST-mm-FMN2-eFSI (GST-FMN2-eFSI) proteins were expressed in Escherichia coli Rosetta bacterial cells (Merck Millipore). Bacteria were cultured in LB medium (100 mg/l ampicillin ...
-
bioRxiv - Biophysics 2020Quote: ... beads were removed and recombinant dynein was concentrated in a 100 KDa cut-off filter (Amicon Ultracel, Merck-Millipore) to a final volume of 500 μL ...
-
bioRxiv - Microbiology 2023Quote: ... were incubated for 2 h at room temperature with the respective recombinant glycosyltransferase enzymes (6.3 µg/ mL) and UDP-GlcNAc (2 mM, Merck) in glycosylation buffer (15 mM HEPES ...
-
bioRxiv - Systems Biology 2022Quote: ... The membrane was blocked with blocking buffer (2.5 % m/v) BLOT-Quick Blocker (Merck) in PBST (Phosphate-buffered saline with Tween ...
-
bioRxiv - Plant Biology 2021Quote: Dried samples were mineralized in 400 μL of 65% (w/v) HNO3 (Suprapur, Merck) for 3 h using a HotBlock CAL3300 (Environmental Express ...
-
bioRxiv - Molecular Biology 2019Quote: ... The supernatants were kept on ice and neutralized with 20% (w/v) KOH (Merck) and centrifuged at 11,000 g and 4°C for 5 min to sediment the KClO4 salt ...
-
bioRxiv - Physiology 2021Quote: ... a grid was briefly placed on 10 μL 2% uranyl acetate (w/v, Merck). Images were acquired under a JEM-1010 electron microscope (JEOL ...
-
bioRxiv - Cancer Biology 2022Quote: ... cell lines were suspended in 1.2% w/v sterile sodium alginate (Merck, Dorset, UK) in 0.15 M sodium chloride at densities ranging from 6 × 105 cells/ml to 1.2 × 106 cells/ml ...
-
bioRxiv - Cell Biology 2022Quote: ... and blocked in blocking solution (4 % (w/v) skim milk powder (Merck, #70166-500G) and 1 % (w/v ...
-
bioRxiv - Plant Biology 2023Quote: ... then 2% w/v Aspegillus niger pectinase (Merck Life Science UK Ltd., Gillingham, UK) 45°C for 2 hours ...
-
bioRxiv - Immunology 2023Quote: ... cells were fixed by incubation with PBS containing 4% (w/v) paraformaldehyde (Merck, #104005100) for 15 min at room temperature ...
-
bioRxiv - Bioengineering 2021Quote: Three sender area squares (3 × 3 mm) were put into a 24-well containing 300 μL of anti-His antibody (Merck, Germany, NOVG70796-3) (10 μg mL−1 in DPBS ...
-
bioRxiv - Biochemistry 2020Quote: ... The recombinant protein was dialyzed overnight using an Amicon Ultra 15 mL spin dialysis column 10 000 MWCO (Merck, Germany). Purified protein was quantified using Bradford’s assay ...
-
bioRxiv - Cancer Biology 2021Quote: Primary HPMECs in EBM2 medium or ST1.6R cells in DMEM/1% FBS were cultured overnight and stimulated with 2 μg/ml of recombinant TNC (Merck Millipore) for 24 h ...
-
bioRxiv - Neuroscience 2023Quote: ... the affinity resin was prepared by incubating 100 µg of recombinant protein with 20 µl of nickel beads (PureProteome Nickel magnetic beads, Merck) in 50 mM Pull-Down Buffer PDB (Tris–HCl pH 7.4 ...
-
bioRxiv - Biochemistry 2024Quote: ... Affinity-purified recombinant TrK13 proteins (WT, C580Y, R539T and C580Y) or commercially purchased BSA (A7030) or lysozyme (L6876) (Merck, Germany), each at 10 µM concentration ...
-
Appropriate glycemic management protects the germline but not uterine environment in type 1 diabetesbioRxiv - Developmental Biology 2024Quote: ... The dose of COS (75-400 IU daily of recombinant follicle stimulating hormone (Gonal-F Merck or hMG Menopur, Ferring) was individualized to patient’s age ...
-
Appropriate glycemic management protects the germline but not uterine environment in type 1 diabetesbioRxiv - Developmental Biology 2024Quote: ... Ovarian follicle growth tracking was performed by scheduled transvaginal ultrasound examinations that lead to the planning timepoint for oocyte maturation triggered by administration of recombinant hCG (250 microgr SC Ovitrelle; Merck). Oocyte retrieval was carried out by transvaginal ultrasographically-guided follicular puncture 37 hours later.
-
bioRxiv - Cell Biology 2021Quote: Human immortalised articular chondrocyte C28/I2 cells were purchased from Merck Millipore and cultured in DMEM/F12 (Gibco ...