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Citations for Merck :
451 - 500 of 1146 citations for Recombinant Human Serpin Peptidase Inhibitor Clade B ovalbumin Member 9 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... and endpoint B) added with 500 µl of RPMI 1640 culture medium with cyanocobalamin (Merck) to a final concentration of 1 nM ...
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bioRxiv - Biochemistry 2024Quote: ... Calibration of the mass spectrometer was achieved using [Glu1]-Fibrinopeptide B (50 fmol/μL, Merck) and the instrument was operated at a source temperature of 80 °C with the capillary set to 3 kV.
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bioRxiv - Biochemistry 2023Quote: Total cell lysates of HEK293 cells stably expressing C-terminally Flag M2-tagged SIRT4 were incubated overnight at 4°C with anti-Flag M2 agarose beads (Merck, Darmstadt, Germany). The beads were washed three times with washing buffer (50 mM Tris-HCl ...
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bioRxiv - Microbiology 2024Quote: ... HEK293T cells transfected with Flag-tagged NSP1 or vector plasmid were treated with the following compounds for 6 h: 10 μM of MG132 (Merck, Cat# 474787), 50 μM of chloroquine (CQ ...
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bioRxiv - Neuroscience 2023Quote: ... R&D, AF2408), Goat-Anti Human SOX17 (1:100, R&D, AF1924) or Mouse Anti-Human Beta-Tubulin (1:1000, Merck, T8660) primary Chicken Anti-Human MAP2 (1:2000 ...
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bioRxiv - Molecular Biology 2023Quote: ... stained with anti-human IFN-γ and anti-human IL2 antibodies for 40 min at RT in 0.2% saponin (Merck, Germany) in PBS (Gibco ...
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bioRxiv - Molecular Biology 2022Quote: ... and applied directly onto a column of 2 ml pre-equilibrated Ni-NTA beads (cOmplete His-Tag Purification Resin from Merck). After several washes with high salt (500 mM NaCl) ...
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bioRxiv - Molecular Biology 2022Quote: ... the supernatant was loaded on to a gravity flow-based Ni-NTA metal affinity column (2 ml beads, cOmplete His-Tag Purification Resin from Merck), equilibrated and washed with 10 column volumes of buffer A containing 5 mM imidazole ...
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bioRxiv - Molecular Biology 2020Quote: ... WT—or its mutants fused with a His-tag on the amino-terminus—were expressed in Escherichia coli BL21 (DE3) using pET15b vector (Merck). A single colony was inoculated in 2 mL Luria-Bertani broth (LB ...
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bioRxiv - Microbiology 2021Quote: ... The sonicated cellular extract was spun down and the supernatant has been incubated with His-binding resin (Merck 69670-5) for 10-30 min at room temperature ...
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Autorepression of Yeast Hsp70 co-chaperones by intramolecular interactions involving their J-domainsbioRxiv - Biochemistry 2024Quote: ... the supernatant was loaded onto a gravity flow-based Ni-NTA metal affinity column (2 ml beads, complete His-Tag Purification Resin from Merck), equilibrated and washed with 10 column volumes of buffer A containing 5 mM imidazole ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The supernatant was passed through a 0.45 µM syringe filter before transferring into a tube containing HIS-Select Nickel Affinity Gel (Merck India) and kept on a mixer at 4°C for 3 h ...
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bioRxiv - Neuroscience 2020Quote: Recombinant GST-hSLP4A was expressed in and purified from BL21 Rosetta DE3 E.coli (Merck, Ca. No. 70954). 5ml of saturated pDEST15 hSlp4-a-transformed BL21 Rosetta DE3 E.coli culture were diluted in 500 ml 2x YT (Sigma Aldrich ...
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bioRxiv - Immunology 2021Quote: ... recombinant SARS-COV-2 Spike protein was desalted and concentrated with C4 ZipTips (Merck Millipore, Darmstadt, Germany) and spotted on a ground steel target using 2’,5’-Dihydroxyacetophenone ...
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bioRxiv - Cell Biology 2021Quote: ... 1000 units/ml of recombinant Leukemia Inhibitory Factor (LIF, Interchim, #8V6280) and 0.1% β-mercaptoethanol (Merck, #M6250) on gelatin-coated plates ...
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bioRxiv - Microbiology 2019Quote: ... 1 mL of the pre-enriched suspensions for each drinking water sample was added to sterile tubes containing 9 mL of TTB (Merck, South Africa). The suspensions were subjected to incubation at 36 ± 1 °C overnight ...
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bioRxiv - Cell Biology 2024Quote: ... HeLa S3 Flp-In host cells were co-transfected with pEF5/FRT/CTLA4-HA and pOG44 at a ratio of 1:9 using GeneJuice (Merck Millipore, 70967) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: Whole kidneys were homogenised in 250 mM sucrose / 20 mM triethanolamine with protease inhibitors (1% Merck Protease Inhibitor Cocktail III). The homogenate was cleared of large debris by centrifugation at 4000 g for 15 mins at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... Cells were washed with ice-cold PBS and lysed for 5 min on ice in 50 μL RIPA buffer supplemented with protease inhibitors (Protease Inhibitor Cocktail set III, EDTA free, Merck) and phosphatase inhibitors (PhosSTOP ...
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bioRxiv - Molecular Biology 2019Quote: ... and 0.1% Deoxycholate Na salt) supplemented with 1x protease inhibitor cocktail (Protease inhibitor cocktail Set IV in DMSO; Merck, 539136) and 1 mM PMSF ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were lysed in RIPA buffer supplemented with protease and phosphatase inhibitor cocktail and 1 μM PARG inhibitor ADP-HPD (Merck). For chromatin fraction ...
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bioRxiv - Immunology 2019Quote: ... The pre-mRNA splicing inhibitor isoginkgetin from Merck Millipore and the madrasin from Sigma-Aldrich are dissolved in DMSO according to the manufacturer protocol ...
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bioRxiv - Cell Biology 2021Quote: ... cOmplete™ EDTA-free Protease Inhibitor Cocktail (Merck), 200 U/mL RNaseOUT ...
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bioRxiv - Cell Biology 2021Quote: ... cOmplete™ EDTA-free Protease Inhibitor Cocktail (Merck), RNaseOUT recombinant ribonuclease inhibitor (200 U/mL ...
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bioRxiv - Microbiology 2021Quote: ... and an EDTA-free protease inhibitor tablet (Merck). Cells were further lyzed by sonification (10 cycles of 15 s with 10 s resting on ice in between each cycle) ...
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bioRxiv - Biochemistry 2021Quote: ... The proteasome inhibitor MG132 was obtained from Merck/Millipore and used at the final concentration of 20 µM for 3 h ...
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bioRxiv - Biochemistry 2022Quote: ... 1 mM TCEP) supplemented with protease inhibitor (Merck Protease inhibitor cocktail III ...
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HNRNPA2B1 controls an unfolded protein response-related prognostic gene signature in prostate cancerbioRxiv - Cancer Biology 2022Quote: ... The IRE1 inhibitor STF083010 was purchased from Merck Life Science ...
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bioRxiv - Microbiology 2021Quote: ... p38 MAP Kinase Inhibitor BIRB 796 (MERCK Millipore) was used at 10 μM ...
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bioRxiv - Microbiology 2022Quote: ... 10% glycerol with protease inhibitor (EDTA-free, Merck) and lysed by sonication for 14 cycles at 18-micron amplitude ...
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bioRxiv - Physiology 2019Quote: ... and 1% protease inhibitor cocktail (Merck, Darmstadt, Germany). Homogenates were rotated for 40 min at 4°C and subjected to centrifugation (10 000 g for 10 min at 4°C) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... FK506 (inhibitor of FKBP activity, Sigma-Aldrich, Merck), VER (inhibitor of ATP-binding site of Hsp70 ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 μM of the RNAPII inhibitor DRB (Merck) was added to culture medium for 1h ...
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bioRxiv - Developmental Biology 2021Quote: ... the MEK1/2 inhibitor PD0325901 (1 μM, Merck), LDN-193189 (100 nM ...
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bioRxiv - Developmental Biology 2022Quote: ... phosphatase inhibitor cocktail sets II and IV (Merck), and phenylmethylsulfonyl fluoride (PMSF ...
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bioRxiv - Immunology 2022Quote: ... 0.2% v/v protease inhibitor cocktail III (Merck), 2.5% v/v murine RNase inhibitor (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... pH 7.5) supplemented with protease inhibitor cocktails (Merck) and DNase I (Merck) ...
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bioRxiv - Cell Biology 2023Quote: ... 1:250 Protease Inhibitor Cocktail (Merck, P8340-5ML), 1:100 Phosphatase Inhibitor cocktail 3 (Merck ...
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bioRxiv - Cell Biology 2023Quote: ... followed by trypsin inhibitor soya bean (Merck, 10109886001) (250 μg/mL for 15 min) ...
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bioRxiv - Physiology 2023Quote: ... and proteases inhibitors (Roche, cOmplete Merck Life Science). Samples were sonicated three times for 10 seconds using a Branson Ultrasonic Sonifier 250 (Branson ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mM MgCl2 and cOmplete protease inhibitor (Merck), and the cells were homogenized by sonication ...
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bioRxiv - Microbiology 2023Quote: ... inhibitors of the proteasomal (MG132, 10 μM, Merck) and lysosomal degradation pathways (leupeptin ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with protease inhibitor cocktail (Merck Life Sciences). We determined protein concentration with Bradford reagent (Merck Life Sciences) ...
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bioRxiv - Biochemistry 2024Quote: ... supplemented with 1x cOmplete protease inhibitor (4693159001, Merck). Cells were lysed using a Fastprep24 bead beater and the lysate was cleared by centrifugation ...
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bioRxiv - Microbiology 2023Quote: ... and an EDTA-free protease inhibitor tablet (Merck). The resuspended cells were then lysed by sonication (10 cycles of 15 s with 10 s resting on ice in between each cycle) ...
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bioRxiv - Molecular Biology 2023Quote: ... supplied with 1× Complete Protease Inhibitor Cocktail (Merck). Protein concentration was assessed using BCA protein assay (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... and ROCK inhibitor (Y-27632, Merck, Darmstadt, Germany).
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bioRxiv - Genomics 2023Quote: ... 1X cOmplete protease inhibitor cocktail (Merck cat. #11873580001) and the appropriate amount of antibody (1ug of α-H3K27ac and 0.5ug of α-H3K4me1 ...
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bioRxiv - Cell Biology 2023Quote: ... and proteases inhibitors (Roche, cOmplete Merck Life Science). Samples were sonicated three times for 10 seconds using a Branson Ultrasonic Sonifier 250 (Branson ...
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bioRxiv - Molecular Biology 2024Quote: ... and protease inhibitor cocktails (Complete mini, Merck Millipore) using Potter–Elvehjem homogenizers and then further homogenized via passage through a 27-gauge needle ...