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Citations for Merck :
1 - 50 of 367 citations for Recombinant Human Metadherin GST tagged since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 2020) containing 300 ng GST-tagged MP-GAP fragments and 200 ng recombinant human Aurora A kinase (Merck, 14-511), 1mCi of [gamma-P32]ATP (Hartmann Analytic ...
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bioRxiv - Immunology 2020Quote: ... 350 ng/mL active ERK2-GST tagged (Merck) or 175 ng/mL ERK1 plus 175 ng/mL ERK2-GST for 30 min at 30°C.
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bioRxiv - Biophysics 2021Quote: ... 6×His-tagged recombinant MSP1E3D1 (Sigma-Aldrich, Merck, USA) and FLAG-tagged recombinant mCardinal (kindly provided by Jakub Czapiński ...
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bioRxiv - Cancer Biology 2023Quote: ... Immunoprecipitation of cell lysates overexpressing FLAG-tagged or GST-tagged proteins were performed using anti-FLAG-M2 affinity beads (Merck) or glutathione-Sepharose beads (Cytiva).
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bioRxiv - Cell Biology 2020Quote: Recombinant GST-mm-FMN2-eFSI (GST-FMN2-eFSI) proteins were expressed in Escherichia coli Rosetta bacterial cells (Merck Millipore). Bacteria were cultured in LB medium (100 mg/l ampicillin ...
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bioRxiv - Cell Biology 2021Quote: ... human recombinant insulin (100 µg/mL; Merck), recombinant human insulin-like growth factor 1 (hIGF1 ...
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bioRxiv - Molecular Biology 2020Quote: ... Recombinant human TNFα (GF023) was from Merck. Purified anti-His tag mouse antibody was from BioLegend (USA) ...
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bioRxiv - Neuroscience 2020Quote: Recombinant GST-hSLP4A was expressed in and purified from BL21 Rosetta DE3 E.coli (Merck, Ca. No. 70954). 5ml of saturated pDEST15 hSlp4-a-transformed BL21 Rosetta DE3 E.coli culture were diluted in 500 ml 2x YT (Sigma Aldrich ...
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bioRxiv - Developmental Biology 2022Quote: ... 10 mg/mL Human recombinant Insulin (Merck, Cat. #91077C) and 1% Pen/Strep ...
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bioRxiv - Neuroscience 2023Quote: ... Tau ladder (six human tau recombinant isoforms, Sigma-Merck) was used to identify tau isoforms in NCI-H716 cells ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.1 IU/ml recombinant human FSH (Merck & Co., Inc), 0.125 mg/ml recombinant human hyaluronan (Novozymes) ...
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bioRxiv - Immunology 2023Quote: ... The eluted His-Myc tagged recombinants were further concentrated using 3kDa ultra concentration units (Merck, Germany). The GST-tagged recombinant proteins were affinity purified using Glutathione Sepharose 4B beads and purified in batch mode ...
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bioRxiv - Genetics 2020Quote: ... then rhCG (recombinant human chorionic gonadotropin alpha, OVIDREL, Merck Serono) on day 9 Oocytes were collected by laparoscopic follicular aspiration 32–35 h after rhCG administration ...
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bioRxiv - Immunology 2020Quote: ... and recombinant human RAP (Merck Millipore, 200 nM final concentration) were used to stimulate nuclear translocation of MerTK.
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bioRxiv - Biophysics 2022Quote: Lyophilized recombinant human Chorionic Gonadotropin (hCG) (Chorulon Merck #140-927) is reconstituted with the included sterile 1x PBS ...
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bioRxiv - Cell Biology 2023Quote: ... of the following ECM proteins: recombinant human fibronectin (Merck, 341631), recombinant human vitronectin (PeproTech ...
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bioRxiv - Cell Biology 2023Quote: ... Treatments used recombinant human TNFα (10 ng/ml; Merck 654245) and/or LMB (20 ng/ml ...
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bioRxiv - Cell Biology 2024Quote: ... recombinant full-length human active AAK (#14-511 Merck Millipore, Germany) were incubated either in the presence or the absence of 14nM of N-terminal GST-fused active HsMPS1/TTK (SignalChem ...
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bioRxiv - Developmental Biology 2023Quote: ... then 1,000 IU recombinant human chorionic gonadotropin alpha (rhCG, OVIDREL, Merck Serono) was injected on day 9 ...
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bioRxiv - Bioengineering 2021Quote: ... recombinant human platelet derived growth factor (hPDGF-AB, 20 ng/ml, Merck, GF106), recombinant human oncostatin M (hOSM ...
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bioRxiv - Neuroscience 2023Quote: ... and human recombinant FGF-2 (10 ng/ml; Merck Millipore, Burlington, MA, USA) as mitogens ...
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bioRxiv - Immunology 2023Quote: ... Mo were primed with 200 U/mL human recombinant IFNα2β (Intron A; Merck) for 2 h and then stimulated with 20 ng/mL of recombinant human mIL-1β (R&D ...
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bioRxiv - Cell Biology 2022Quote: ... GST-agarose magnetic beads (Merck) were incubated with equimolar concentration of flag-tagged human RNase H1 and GST-CTD proteins in PBS buffer (pH ...
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bioRxiv - Developmental Biology 2024Quote: ... and 0.1 IU/mL recombinant human follicle-stimulating hormone (GONAL-F; Merck, Darmstadt, Germany). The COCs were washed with IVM medium and then incubated in 100 μL of IVM medium (20–25/droplet ...
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bioRxiv - Plant Biology 2021Quote: ... as a translational fusion with glutathione-S-transferase (GST) and purified with GST-Bind resin (Merck), as previously described (Sullivan et al. ...
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bioRxiv - Cell Biology 2023Quote: ... rabbit anti-GST (Merck,#ZRB1223,1:1000), mouse anti-β-actin (Santa Cruz Biotechnology,#SC-47778,1:1000) ...
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bioRxiv - Microbiology 2024Quote: ... Active human recombinant caspase-3 (#CC119) and caspase-9 (#CC120) proteins were purchased from Merck.
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bioRxiv - Biochemistry 2023Quote: ... Rgl2RA-Halo or GST- Rgl2RA/GST-BRAFRBD fusion proteins were concentrated using a concentrator (10 KDa MWCO, Merck, Amicon Ultra centrifugal filters ...
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bioRxiv - Genetics 2020Quote: ... which was performed by intramuscular injection with rhFSH (recombinant human follitropin alpha, GONAL-F, Merck Serono) for 8 days ...
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bioRxiv - Neuroscience 2024Quote: The inhibitory activity of novel compounds against human recombinant AChE (hAChE, E.C. 3.1.1.7, purchased from Merck) and human plasmatic butyrylcholinesterase (hBChE ...
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bioRxiv - Neuroscience 2021Quote: ... Injections of 50 µl NGF at a concentration of 0.8 µM (NGF, human recombinant, Calbiochem, Merck, Germany) dissolved in phosphate buffer saline (PBS ...
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bioRxiv - Developmental Biology 2023Quote: ... 400 µl of solution of recombinant TnC (Supplementary Materials and Methods) or purified human TnC (Merck Millipore) (15 µg/ml in 0.01% PBS-Tween ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with 10 ng/ml of the recombinant human TNFα protein (Merck-Millipore, Cat# GF023) and the outcome was assessed at RNA or protein levels.
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bioRxiv - Plant Biology 2021Quote: ... and detected with anti-GST monoclonal antibody (Merck), anti-GFP-HFP monoclonal antibody (Miltenyi Biotech) ...
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bioRxiv - Immunology 2022Quote: ... Animals were randomly assigned to be treated with either 170,000 IU/kg human recombinant interferon alpha-2a (Merck), or BSA/saline (0.9% NaCl ...
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bioRxiv - Cell Biology 2022Quote: ... The eluate was concentrated down to 1.5 mL using an Amicon Ultra Centrifuge Filter unit (30 kDa cutoff for GST-UtrCH-sfGFP and 10 kDa cutoff for GST-LifeAct-sfGFP; Merck, UFC903024 and UFC901024, respectively). The GST tag was removed by digestion with 8 units/mL PreScission Protease (Cytiva ...
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bioRxiv - Developmental Biology 2021Quote: ... 500 μg/mL rice-derived recombinant human albumin and 213 μg/mL L-ascorbic acid 2-phosphate (both from Merck). After 24 h of CHIR99021 stimulation ...
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bioRxiv - Biophysics 2021Quote: ... This mix included 98% unlabeled recombinant human E254N tubulin diluted in assay buffer containing oxygen scavengers (180 mg/ml catalase (C40, Merck) and 752 mg/ml glucose oxidase (22778.01 ...
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bioRxiv - Molecular Biology 2021Quote: ... or already HRP-conjugated antibody (anti-GST-HRP, Merck RPN1236) was added for 1 h at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... The clarified supernatant was incubated with GST-agarose beads (Merck) overnight ...
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bioRxiv - Developmental Biology 2023Quote: ... healthy female cynomolgus monkeys were subjected to follicular stimulation by intramuscular injection of 20 IU of recombinant human follitropin alpha (rhFSH, Gonal F, Merck Serono) for 8 days ...
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bioRxiv - Bioengineering 2024Quote: ... We mixed a fluorescently tagged collagen (C4361, Merck) with untagged collagen 3mg/ml to visualise the cellular interactions with collagen fibres and their alignment ...
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bioRxiv - Plant Biology 2021Quote: ... 14-3-3 binding was detected using anti-GST monoclonal antibody (Merck).
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bioRxiv - Microbiology 2020Quote: ... GST bound protein was then cleaved using biotinylated thrombin (Merck Millipore; 69672) according to the manufacturers instructions overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Recombinant BG4 (recBG4) (Millipore Merck) was added as control.
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bioRxiv - Biochemistry 2020Quote: ... Primary antibodies were used as follows: GST (1:1,000 dilution; Cat # 27457701V) from Merck, ubiquitin (1:1,000 dilution ...
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bioRxiv - Biochemistry 2022Quote: ... and both His6 and GST tags were cleaved with 5-10 unit thrombin (Merck) per milligram of protein for 24 h at 4°C and subjected to the SEC using the superdex 200 10/30 GL column ...
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bioRxiv - Cell Biology 2022Quote: ... Equal loading of the GST-UBAN beads was confirmed using ponceau S (Merck KGaA) staining.
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bioRxiv - Cell Biology 2021Quote: ... Myc-tagged proteins were immuno-precipitated with the 9E10 antibody (Merck); Rpb1 was immunoprecipitated using the 8WG16 antibody (Merck).
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bioRxiv - Biophysics 2024Quote: ... GST-ASF1A variants were concentrated using Amicon Ultra Centrifugal Filter 30 kDa MWCO (Merck Millipore) to a 2.5 mg/mL ...