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Citations for Merck :
351 - 400 of 489 citations for Recombinant Human Interleukin 7 Receptor Fc His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... and additional wells with 20% biotinylated PLL-g-PEG and FNIII(7-10) were supplemented with 1 µM staurosporine (Merck Millipore) to serve as a positive control for apoptosis ...
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bioRxiv - Cancer Biology 2022Quote: ... The purified protein was dialyzed overnight in 25 mM Tris-Cl pH 7.5 using SnakeSkin®ialysis tubing (7 kDa molecular weight cutoff) and concentrated using Amicon™ Ultra-15 Centrifugal Filter Units (30 kDa molecular weight cutoff) (Merck) according to the requirements of the experiments.
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bioRxiv - Microbiology 2022Quote: A CRISPR array containing 6 identical spacers targeting the tetR gene flanked by 7 repeats was cloned into pCDF-Duet (Novagen, Merck Millipore) by ligation after NcoI and SalI digestion ...
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bioRxiv - Microbiology 2023Quote: ... The pre-cleared lysates were then incubated overnight at 4°C with polyclonal rabbit anti-TipR antibodies (1:400 dilution) (Kirkpatrick and Viollier, 2014) or monoclonal rabbit anti-HA antibodies (1:250 dilution) (Clone 114-2C-7, Merck Millipore). ...
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bioRxiv - Neuroscience 2024Quote: ... for 5 x 7 min and incubated 2h at room temperature (RT) with blocking solution containing 10% normal donkey serum (NDS, Merck Millipore), 3% Bovine serum albumin (BSA ...
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bioRxiv - Immunology 2023Quote: ... The digested suspension was pelleted and the pepM extract buffer exchanged to PBS pH 7 using 10 kDa Amicon centrifugal filter unit (Merck, Ireland). PepM extracts were confirmed with SDS-PAGE and stored in solution at − 20°C.
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bioRxiv - Microbiology 2023Quote: ... The flow-through was sterile filtered and concentrated to a final volume of 7 ml using Amicon Ultra-15 Centrifugal filter concentrator (Merck Millipore).
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bioRxiv - Molecular Biology 2020Quote: ... Fractions containing the respective recombinant proteins were pooled and concentrated using Amicon® Ultra-15 spin concentrators with 10 kDa cut-off (Merck Millipore). Concentrated protein pools were run on a Superdex 200 Increase 10/300 GL ...
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bioRxiv - Immunology 2020Quote: ... urinary human menopausal gonadotropin (hMG) (HMG-lepori 75 UI, Angelini Farmacéutica, S.A, Spain) and recombinant hCG (Ovitrelle 250 micrograms, Merck Serono Europe Limited, Spain) or luprorelin acetate (Procrin 1 mg/0,2 ml ...
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bioRxiv - Neuroscience 2021Quote: ... and 10 ng/ml human leukaemia inhibitory factor (hLIF; Merck KGaA, Hesse, Germany) for 12 days ...
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bioRxiv - Immunology 2019Quote: ... anti-human C5aR or nonpeptide C5aR antagonist W-54011 (5 μM, Merck Millipore)(25 ...
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bioRxiv - Cancer Biology 2019Quote: ... Sections were then incubated with mouse anti-human mitochondria (clone 113-1, Merck) diluted in blocking solution at a dilution of 1:1000 at 4°C overnight ...
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bioRxiv - Neuroscience 2020Quote: Immortalized human cerebral microvascular endothelial cell line (hCMEC/D3) was purchased from Merck-Millipore (Darmstadt ...
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bioRxiv - Microbiology 2022Quote: ... microtiter plates coated with either human IgM (30 ng/ml) (Merck, No I8260) for the CP ...
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bioRxiv - Immunology 2020Quote: ... Rabbit anti-human CD11c (ab216655) underwent buffer exchange using 50kDa amicon filters (Merck) so as to place antibodies in 0.1M NaHCO3 ...
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bioRxiv - Neuroscience 2023Quote: ... The Luminex Milliplex® MAP human cytokine / chemokine magnetic bead panel (HSTCMAG28SK07; Merck) was used to detect cytokines ...
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bioRxiv - Microbiology 2020Quote: ... Proteins in the serum were removed by passing it through a pre-rinsed (7 times washed) Amicon Ultra-2ml 3000 MWCO (Merck Millipore, USA) column ...
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bioRxiv - Neuroscience 2020Quote: ... The cortices were homogenized mechanically in PBS by pipetting up and down with a 5mL plastic pipette, centrifuged (500 x g, 7 min, 4 °C) and resuspended in DMEM (Sigma-Aldrich, Merck, Switzerland) containing 5% FCS (Biochrom ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... 50 µL benzaldehyde (BA) and 250 µL 3-octanol (OCT) (CAS 100-52-7, and 589-98-0, respectively; Merck, Darmstadt, Germany) were applied undiluted to 1-cm-deep Teflon containers of 5 and 14 mm diameter ...
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bioRxiv - Molecular Biology 2021Quote: ... the protocol described by Choi et al.65 was applied using home-made wash buffer (50% formamide (Merck Millipore, 75-12-7), 5XSSC (Molecular Biology-P ...
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bioRxiv - Microbiology 2022Quote: ... Five duplicate plates were spread individually by six gradient diluted soil suspensions from 10−2 to 10−7 dilution on 1/10 TSA medium (Tryptic Soy Agar, Merck, Darmstadt, Germany) and incubated under both aerobic and anaerobic conditions at 28 °C for two weeks ...
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bioRxiv - Genomics 2023Quote: ... These assessments were conducted on 7-day old Foc strains cultured on petri dishes with potato dextrose agar (PDA; Merck, Darmstadt, Germany). An approximately 5 mm piece of the actively growing colony from each isolate was placed on PDA plates and incubated at 27°C ...
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bioRxiv - Neuroscience 2023Quote: ... Flies were raised at 25℃ and collected on the day of eclosion and kept in the dark for 7-10 days on the fly media containing 0.4 mM all trans-Retinal (Merck, St. Louis, MO). The experiments were performed with the “8-well” chamber (16 mm diameter x 10 mm height ...
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bioRxiv - Cancer Biology 2024Quote: ... Two sets of four EV-rich fractions (7–12 and 16-22) were pooled and concentrated using Amicon Ultra-4 10 kDa centrifugal filter device (Merck Millipore, USA). Alternatively ...
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bioRxiv - Bioengineering 2021Quote: ... the chemically decellularized aortas were treated with 900 units (25 μg/mL DNase I recombinant, RNase free, Cat. No. 4716728001, Roche, Merck KGaA, Darmstadt, Germany) using a total of 3 DNase treatment for 2 hours each to achieve the desired level of DNA assessed by (Quant-iT™ PicoGreen™ dsDNA Assay Kit ...
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bioRxiv - Immunology 2022Quote: ... the cells were transferred to a single well containing a monolayer of mitomycin C (Cat no. 50-07-7, Millipore Sigma, Merck, Darmstadt, Germany)-treated OP9/ OP9-DL1 cells or were kept unaltered ...
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bioRxiv - Biochemistry 2021Quote: ... a biotinylated DNA oligonucleotide complementary to the target tRNA (Supplementary Table 7) was immobilized on Novagen Streptavidin Agarose resin (Merck-Millipore, Burlington, MA) in a 0.22 μm Ultrafree-MC filter cup (Merck-Millipore ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 mM EDTA) with 1 µl lyticase (17 U/ µl in 10 mM KPO4 pH 7, 50% glycerol, Merck >2000 U/mg L2524), heated to 50°C for 2 min before addition of 40 µl molten CleanCut agarose (Bio-Rad 1703594) ...
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bioRxiv - Molecular Biology 2022Quote: ... was placed on the top of a water-cooled glass column (33 × 2.5 cm) filled with a slurry of silica gel 60 (with the addition of 7 % water, 40 – 63 μm, Merck, Darmstadt, Germany, # 1.09385.2500) and n-pentane ...
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bioRxiv - Biochemistry 2024Quote: Mixing of lysozyme crystals (7×2 mm; grown by batch crystallisation) with 25 mM sulfanilic acid azochromotrop (SAA, Merck, lmax 505–510 nm), a highly absorbing red dye ...
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bioRxiv - Neuroscience 2021Quote: ... Anti-human Aβ mouse monoclonal antibody (clone W0-2; Merck Chemicals GmbH, Darmstadt, Germany) and rabbit anti-β-actin monoclonal antibody (clone 13E5 ...
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bioRxiv - Developmental Biology 2021Quote: ... and seeding on 16.7 µg/ml human plasma fibronectin-coated plates (Merck Millipore, FC010). After 48h ...
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bioRxiv - Bioengineering 2021Quote: ... Human plasminogen-depleted fibrinogen was utilized for hydrogel preparation when stated (Merck, Darmstadt, Germany). Aprotinin was purchased from R&D Systems (Minneapolis ...
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bioRxiv - Zoology 2021Quote: C-peptide and glucocorticoid levels were determined using a radioimmunoassay (Human C-Peptide, Merck Millipore ...
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bioRxiv - Cell Biology 2022Quote: ... Mission siRNA for human Pacsin 2 siRNA (SASI_Hs01_0021-5538, SASI_Hs01_0021-5539 and SASI_Hs01_0021-5540, Merck) or MISSION siRNA Universal Negative Control #1 (SIC-001 ...
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bioRxiv - Synthetic Biology 2022Quote: ... Calibration curve samples were generated by diluting the International Standard with human plasma (Merck) to create a standard curve ranging from 955 000 IU/mL to 95.5 IU/mL with three technical replicates of each concentration ...
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bioRxiv - Cell Biology 2023Quote: ... anti-human kindlin2 (mouse monoclonal, 3A3, Merck, MAB2617; WB 1:1000, IF 1:200), anti-mouse kindlin2 (rabbit polyclonal ...
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bioRxiv - Cell Biology 2024Quote: Human embryonic kidney (HEK293) cells were cultured in Dulbeccoʹs Modified Eagleʹs Medium (DMEM, Merck) containing 10% Fetal Calf Serum and 1% Antibiotic Antimycotic Solution (Sigma Aldrich) ...
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bioRxiv - Developmental Biology 2023Quote: ... injection of 5 IU of human chorionic gonadotrophin (hCG; Pregnyl, Merck, Kilsyth, VIC, Australia) 48 h later.
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bioRxiv - Molecular Biology 2021Quote: ... and resuspended in 60 μl PFGE wash buffer containing 1 μl lyticase (17 U/μl 10 mM KPO4 pH 7, 50% glycerol Merck L2524 >2000 U/mg) then incubated for 10 min at 50°C ...
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bioRxiv - Physiology 2020Quote: ... Cows treated with the 7-day CO-Synch + CIDR protocol (n = 769) received administration of 100 μg gonadorelin acetate (GnRH; Fertagyl, Merck Animal Health, Madison, NJ) and insertion of a 1.38 g intravaginal progesterone releasing insert (CIDR® Zoetis ...
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bioRxiv - Microbiology 2020Quote: ... 48 and 72 hours) of exposure to simulated solar radiation was performed by using 2’,7’-Dichlorodihydrofluorescein diacetate (DCFH-DA) (Sigma Aldrich-Merck KGaA, Darmstadtm Germany) solubilized in ethanol ...
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bioRxiv - Cell Biology 2023Quote: ... to validate the gene expression the plasmid (vide-supra) was transfected into HEK293 cells using PEI (Sigma-Aldrich/Merck Cat. No. 49553-93-7) following an optimized version of the standard protocol(Rajendra ...
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bioRxiv - Microbiology 2024Quote: ... 15 µg of total protein in a volume of 47 µl 1x sample buffer was treated with 7 U of benzonase (#70746; Merck Millipore, Burlington, MA, USA) in dilution buffer (20 mM HEPES pH 8.0 ...
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bioRxiv - Bioengineering 2021Quote: ... Part A consisted of 20% hPL (v/v) and 4mg/mL human fibrinogen (Merck, USA) in DMEM/F12 (Life Technologies ...
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bioRxiv - Cell Biology 2019Quote: ... They were coated for 1 hour with 50 μg/mL human plasma fibronectin (Merck Millipore) diluted in PBS and washed three times with PBS.
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bioRxiv - Immunology 2021Quote: ... MIP-1β by MILLIPLEX MAP Human High Sensitivity T Cell Panel (Merck Cat# HSTCMAG-28SK) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... Two hundred microliters of medium containing 2 μM human insulin (Merck, Sigma-Aldrich Cat# I9278) or medium only was added to the thoraxes for 10 min (the final concentration of insulin was 1 μM) ...
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bioRxiv - Microbiology 2023Quote: ... and NTK seeded on surfaces coated with 100 μg/ml type IV human collagen (Merck) in defined keratinocyte serum-free medium (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... All human cell lines were cultured at 37℃ in 5% CO2 in DMEM media (Merck) containing 1% antibiotic (Gibco ...