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Citations for Merck :
451 - 500 of 1343 citations for Recombinant Human GPC3 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... proteins were reduced with 20 mM dithiothreitol (Merck Darmstadt, Germany) at 45°C for 30 minutes ...
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Targeting adipocyte ESRRA promotes osteogenesis and vascular formation in adipocyte-rich bone marrowbioRxiv - Molecular Biology 2023Quote: ... proteins were transferred onto a PVDF membrane (Merck Millipore #IPFL00010) and blocked with 5% skimmed milk-PBST ...
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Docking Domain Engineering in a Modular Polyketide Synthase and its Impact on Structure and FunctionbioRxiv - Biochemistry 2023Quote: ... Proteins were concentrated with Amicon Ultra centrifugal filters from Merck Millipore ...
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bioRxiv - Biophysics 2023Quote: ... After transferring the proteins onto a PVDF membrane (Merck Millipore), the membranes were blocked in TBS-T (20 mM Tris ...
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bioRxiv - Molecular Biology 2023Quote: ... Eluted proteins were concentrated using Amicon spin concentrators (Merck Millipore) and subjected to gel filtration (Superdex 75 and 200 16/60 pg ...
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bioRxiv - Immunology 2023Quote: ... Separated proteins were transferred to PVDF membranes (0.45 mm, Merck) by semi-dry transfer ...
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bioRxiv - Neuroscience 2023Quote: ... the proteins were transferred into nitrocellulose membranes (Merck-Millipore, Germany) for 2h at 300 mV using an electrophoretic transfer system (BioRad) ...
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bioRxiv - Biophysics 2023Quote: ... All protein preparations were concentrated using Amicon Ultracentrifugal filters (Merck), reduced with DTT and purified by reversed-phase high-performance liquid chromatography (RP-HPLC ...
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bioRxiv - Plant Biology 2023Quote: ... Protein extracts were supplemented with 0.5 mM cycloheximide (CHX; Merck), and the mixtures were then divided in two parts ...
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bioRxiv - Molecular Biology 2023Quote: ... 30 μL Magna ChIP™ protein G magnetic beads (Merck) per IP were washed with PBS-Tween 0.1% three times ...
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bioRxiv - Neuroscience 2023Quote: ... We determined protein concentration with Bradford reagent (Merck Life Sciences). We treated lysate samples (20 µg ...
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bioRxiv - Genomics 2023Quote: ... per 20 μl of protein A/G MagnaBeads (Merck Millipore). Beads were then washed once with low salt wash buffer (0.1% SDS ...
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bioRxiv - Neuroscience 2024Quote: ... proteins were transferred to Immobilon-FL PVDF membranes (Merck-Millipore) and washed twice in TBS buffer followed by incubation in Intercept-TBS Blocking Buffer (Li-COR ...
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bioRxiv - Microbiology 2024Quote: ... the pan-protein labelling reagent Atto-NHS ester 594 (Merck) was used (10 μg/ml in PBS ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were then transferred to PVDF membranes (Immobilon-P, Merck) using wet transfer (1h 100V) ...
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bioRxiv - Cancer Biology 2024Quote: ... Chemiluminescent protein detection reagents were applied for GAPDH (Merck, GERPN2232) and laminin receptors (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were blotted on a PVDF membrane (Merck Millipore, IPVH00010). Membranes were incubated in primary antibodies mouse anti-Vinculin (Sigma ...
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bioRxiv - Immunology 2021Quote: ... The MILLIPLEX®MAP Human Isotyping Magnetic Bead Panel-Isotyping Multiplex Assay (HGAMMAG-301K-06, Merck) was used to measure IgG1 ...
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bioRxiv - Immunology 2019Quote: ... The MILLIPLEX®MAP Human Isotyping Magnetic Bead Panel-Isotyping Multiplex Assay (HGAMMAG-301K-06, Merck Millipore Corporation ...
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bioRxiv - Cell Biology 2019Quote: ... Complement components in the cellular supernatant were quantified using the MILLIPLEX MAP Human Complement Panel (Merck). The read out of the multiplex assay was performed in a Magpix instrument (Luminex).
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bioRxiv - Microbiology 2023Quote: ... and mouse anti-human TMPRSS2 (monoclonal, clone P5H9-A3, cat num: MABF2158, 1:500, MERCK, USA) for a minimum of 2 h at RT ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mg/l hydrocortisone and 1 µg/l and human epidermal growth factor (hEGF) (both Merck). To determine EC sex ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Human Caco-2 colorectal adenocarcinoma cells (1.7×105 cells/cm2) were cultured in DMEM medium (Merck) containing 10% FCS at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2024Quote: ... medium was further exchanged with FibroGRO™ Complete Media Kit for Culture of Human Fibroblasts (Merck), consisting of a fibroblasts-specific basal media supplemented with P/S (0.5%) ...
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bioRxiv - Neuroscience 2019Quote: Plasma levels of adiponectin and leptin were measured using commercially available kits (Human Leptin Enzyme Immunoassay, Merck, Cat ...
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bioRxiv - Microbiology 2021Quote: ... Human embryonic kidney (293T) cells were maintained in the Dulbecco’s Modified Eagle Medium (D6046, Merck, Darmstadt, Germany) supplemented with 10% fetal bovine serum (FB-1365 ...
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bioRxiv - Bioengineering 2021Quote: The sEVs used to validate the platform were collected from a commercial human serum purchased from Merck Millipore (same lot in all preparations ...
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bioRxiv - Neuroscience 2021Quote: ... The concentrations of Aβ40 and Aβ42 were determined in brain lysates using the ELISA kits according to the manufacturer’s instructions (human Aβ40 and Aβ42 brain ELISA, Merck).
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bioRxiv - Genomics 2022Quote: ... females and males were primed with 20 U and 10 U human chorionic gonadotropin (hCG, Pregnyl, Merck), respectively ...
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bioRxiv - Immunology 2022Quote: ... Human peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll density gradient centrifugation (GE-171440-02, Merck), collected from the interphase ...
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bioRxiv - Neuroscience 2022Quote: ... C3 and C1 were analyzed with HCMP2MAG-19K-02 Human Complement Magnetic Bead Panel 2 (Merck Millipore). Supernatants from non-stimulated CTL and L2-PD astrocytes cultured for 14 days were collected and stored at −80°C for long storage ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were sub-cultured on days 4 and 8 of differentiation onto human fibronectin (Merck Millipore, #FC010) coated plates at a ratio of 1:4 ...
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bioRxiv - Biochemistry 2023Quote: ... Antibodies usedwere: mouse monoclonal (BIII-136) anti human Band 3 (B9277, Merck Life Science S.r.l., Milan, Italy). Mouse monoclonal (H68.4 ...
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bioRxiv - Cell Biology 2023Quote: ... fully mature Xenopus females were injected with 260 U of human chorionic gonadotropin (hCG; Merck, Ovitrelle 250G) into the dorsal lymph sac about 12-16 h before use and kept overnight at 18 °C ...
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bioRxiv - Biophysics 2021Quote: ... Pooled protein fractions were concentrated with Amicon-Ultra-15 (Merck KGaA) to 11.2 mg ml−1 as measured by the absorbance at 280 nm ...
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bioRxiv - Cell Biology 2020Quote: ... Separated proteins were transferred to PVDF-FL transfer membrane (Merck Millipore) at 40V for 90 min at 4 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein concentrations were determined by Direct Detect® Infrared Spectrometer (Merck).
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bioRxiv - Cell Biology 2022Quote: ... was used to transfer proteins to PVDF Immobilon membranes (Merck Millipore). Blocking and antibody incubation steps were performed in EveryBlot Blocking Buffer (BioRad) ...
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bioRxiv - Molecular Biology 2019Quote: Protein concentration was measured using a Direct Detect Infrared Spectrometer (Merck). 20 µg of proteins were separated on a NuPAGE 4-12% Bis-Tris gel (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were electro-transferred to PVDF membranes (Merck Millipore, MA, USA). Immuno-detection was performed with polyclonal antibodies ...
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bioRxiv - Neuroscience 2020Quote: ... the protein solution was filtered through 100 kDa filter (MERCK, MRCFOR100). The filtrate was transferred to a low-protein binding tube and 30 equivalents of HNE (Cayman Chem ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was concentrated in Amicon centrifugal filters (10 KDa, Merck) in presence of 1:10 GCDCA ...
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bioRxiv - Cell Biology 2021Quote: ... Immunoreactive proteins were visualized by Immobilion western chemiluminescence substrate (Merck-Millipore). Densitometry was carried out by FIJI.
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bioRxiv - Cancer Biology 2019Quote: ... then incubated in lysis buffer containing Protein G Sepharose beads (Merck) and an antibody directed against DDR2 overnight at 4°C with rocking ...
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bioRxiv - Cell Biology 2021Quote: ... The proteins were transferred to an Immobilon-P Transfer Membrane (Merck), blocked with 10% Blocking One (nacalai tesque) ...
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bioRxiv - Biochemistry 2020Quote: ... The proteins were then transferred to a PVDF membrane (Merck Millipore) using a semi-dry western blot transfer system set to a constant current of 200 mA for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... proteins were transferred onto the PVDF membrane (Immobilon-FL, Merck-Millipore). After the electrotransfer ...
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bioRxiv - Physiology 2021Quote: ... breast cancer resistance protein (Bcrp-1:100; Merck Millipore, Massachusetts, USA) or Abcg1 (1:200 ...
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bioRxiv - Cell Biology 2020Quote: Proteins were transferred onto a PVDF membrane (Immobilon-P, Merck Millipore) using wet transfer ...
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bioRxiv - Biophysics 2022Quote: ... The proteins were concentrated using the Amicon Ultra centrifugal filters (Merck) by replacing the buffer with 20 mM Tris-HCl (pH 7.4) ...