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Citations for Merck :
51 - 100 of 2367 citations for Recombinant Human Chemokine C X C Motif Ligand 10 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Protein samples were digested sequentially with Lys-C and trypsin on Microcon-10 filters (Merck Millipore, #MRCPRT010 Ultracel YM-10). The mixture was centrifuged at 1000 rcf for 5 min ...
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bioRxiv - Cell Biology 2022Quote: ... The whey supernatants were concentrated by centrifugation at 4,000 x g at 20°C using Amicon 100-kDa centrifugal filter units (Merck Millipore) to a final volume of ∼6 mL ...
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bioRxiv - Developmental Biology 2023Quote: ... then 1,000 IU recombinant human chorionic gonadotropin alpha (rhCG, OVIDREL, Merck Serono) was injected on day 9 ...
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bioRxiv - Cell Biology 2020Quote: ... and total c-Src (Merck Millipore) antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... cells were treated with 10 μM DAPT and 5 μM Cytarabine (ARA-C) (Merck, Cat#C3350000) for 24 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... Harvested DIP material was clarified (3000 × g, 10 mins and 4 °C) and sucrose (Merck, #84097) was added at a final concentration of 4 % ...
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bioRxiv - Biochemistry 2022Quote: ... Immobilized pepsin was removed by centrifugation at 1,000 x g for 30 sec at 0 °C using a 0.65 µm filter inlet (Merck Millipore, Darmstadt, Germany) and the flow through was injected immediately into the HPLC-MS/MS system ...
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bioRxiv - Immunology 2020Quote: ... samples were centrifuged at 22000 x g for 20 minutes at 4 °C and the supernatant was filtered through Millex GV 220 nm filter (Merck-Millipore). For acid treatment acetic acid was used with 0.1 N final concentration ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were centrifuged (1500 g for 5 min at 4°C) and resuspended in 3 x PCV buffer A + 0.1% NP40 (Merck Life Science). After 10 further min on ice ...
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bioRxiv - Immunology 2021Quote: ... After digestion for 2 min in a water ice bath the samples were separated by centrifugation at 1000 x g for 30 s at 0°C using a 22 µm filter (Merck, Millipore) and were immediately analyzed by LC-MS ...
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bioRxiv - Cell Biology 2022Quote: ... the samples were first concentrated by centrifugation at 4,000 x g at 21°C using Amicon® Ultra-4 Centrifugal Filter Unit with 100kDa molecular weight cutoff (UFC810024, Merck Millipore ...
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bioRxiv - Neuroscience 2022Quote: ... Unencapsulated molecules were removed by washing at least 4 times the LPs suspensions with PBS pH 7.4 for 20 min at 4°C and 5000 x g using an Amicon Ultra-4 concentrator with 100 kDa cutoff (Merck Millipore). The degree of encapsulation was calculated by subtracting from the total mass of the molecule to be encapsulated that present in the flow-through and was found to be higher than 75% in all LP preparations.
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bioRxiv - Bioengineering 2021Quote: ... recombinant human platelet derived growth factor (hPDGF-AB, 20 ng/ml, Merck, GF106), recombinant human oncostatin M (hOSM ...
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bioRxiv - Immunology 2023Quote: ... Mo were primed with 200 U/mL human recombinant IFNα2β (Intron A; Merck) for 2 h and then stimulated with 20 ng/mL of recombinant human mIL-1β (R&D ...
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bioRxiv - Microbiology 2021Quote: Peptides EcFtsN1-32-C and VmFtsN1-29-C were labelled with maleimide-Atto 495 (Merck Life Science UK). EcFtsA1-405 and VmFtsA1-396 were buffer exchanged into binding buffer (50 mM HEPES/KOH ...
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bioRxiv - Microbiology 2021Quote: Peptides EcFtsN1-32-C and VmFtsN1-29-C were labelled with maleimide-Atto 495 (Merck Life Science UK). EcFtsA1-405 and VmFtsA1-396 were buffer exchanged into binding buffer (50 mM HEPES/KOH ...
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bioRxiv - Cell Biology 2021Quote: ... ligation for 30 min 37 °C and polymerase reaction overnight at 37 °C according to manufacturer’s protocol (Merck, Duolink® In Situ Detection Reagents Red ...
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bioRxiv - Cell Biology 2021Quote: ... and c-Fos (PC05; Merck; 1:200). Nuclei were visualized with 4’,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Microbiology 2022Quote: ... and Compound C were procured from Merck Millipore.
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bioRxiv - Pathology 2023Quote: ... anti-surfactant protein C (SFTPC, AB3786, Merck), homemade anti-α-smooth muscle actin (clone 1A4 ...
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bioRxiv - Neuroscience 2020Quote: ... The cortices were homogenized mechanically in PBS by pipetting up and down with a 5mL plastic pipette, centrifuged (500 x g, 7 min, 4 °C) and resuspended in DMEM (Sigma-Aldrich, Merck, Switzerland) containing 5% FCS (Biochrom ...
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bioRxiv - Genomics 2021Quote: ... Each lobe was segmented so cryosections would fit on the 6,200 x 6,400 µm areas of the Codelink-activated microscope slides and frozen in −30°C 2-Methylbutane (Merck, cat.no.: M32631-1L). The frozen liver samples were embedded in cryomolds (10×10 mm ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were permeabilized for 10 minutes in 0.5% Triton X in PBS and incubated for 48 hours at 4°C in rabbit anti-NG2 (AB5320, Merck Millipore; 1:500) and rat anti-MBP (MCA409 ...
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bioRxiv - Biophysics 2019Quote: ... reduced and alkylated protein extracts were digested sequentially with Lys-C and trypsin on Microcon-10 filters (Merck Millipore, # MRCPRT010 Ultracel YM-10). Digested samples were desalted using ZipTips according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... One strain tube thawed rapidly at 37 °C and cultured in 10 ml Brain heart infusion (BHI - Merck) at 37 °C over 24 hours ...
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bioRxiv - Cell Biology 2022Quote: ... and concentrated at 4 °C approximately 300 times using a 10 kDa Centricon Plus-70 centrifugal unit (Merck Millipore ...
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bioRxiv - Cell Biology 2023Quote: ... for 1 h at 4°C then moved to 37°C and incubated for 1 h with or without 100 µM cytochalasin D (Merck), 100 µM jasplakinolide (Abcam ...
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bioRxiv - Cell Biology 2023Quote: ... the supernatants of lysed cells were incubated in shake tubes over 16 h at 4°C with c-Myc antibodies (9E10 M5546, Merck). Then ...
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bioRxiv - Immunology 2024Quote: ... Clear whole-cell extracts were prepared by centrifugation at 20000g for 20min at 4°C and incubated with EZview Red Anti-c-Myc-Affinity gel (Merck) for overnight ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and rabbit anti-prosurfactant protein C (Merck, Germany)) both diluted 1:200 in 0.1% BSA and 0.1% Triton (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 mM sodium pyruvate (Merck TMS-005-C), 2.5 g/L glucose (Sigma G8769) ...
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bioRxiv - Neuroscience 2023Quote: ... c- Fos anti-rabbit (1:200, Merck, ABE457), Gephyrin anti-Mouse-IgG1 (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... and 60 μg/ml catalase (Merck, C-40).
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bioRxiv - Plant Biology 2023Quote: ... 4°C (Merck 3-16KL, KGaA®, Germany). Supernatant was collected and filtered through PTFE membrane filter (0.2 μm ...
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bioRxiv - Neuroscience 2024Quote: ... Laminin A/C (#SAB4200236, Merck or #ab108595, Abcam), LRP-1 (#ab92544 ...
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bioRxiv - Developmental Biology 2021Quote: ... after which they were washed three times in PBST and blocked overnight at 4°C in 10% serum (Merck) in PBS/0.3% TritonX100 ...
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bioRxiv - Immunology 2022Quote: ... For intracellular cytokine labeling cells were incubated for 3 h at 37°C in RPMI-1640+10% FBS with PMA (10μg/ml, Merck), Ionomycin (1μg/ml ...
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bioRxiv - Cell Biology 2022Quote: ... MLg mouse lung fibroblasts (CCL-206, ATCC) were proliferation-inactivated by incubation in culture medium containing 10-μg·ml-1 mitomycin C (Merck) for 2 h ...
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bioRxiv - Microbiology 2023Quote: The culture supernatants containing the secreted proteins were centrifugated (1 h, 30,000xg, 10 °C) and sterilized by filtration (0.22 µm; Express Plus; Merck Millipore). The supernatants were equilibrated to pH 7 with 0.5 mM NaOH and the proteins were purified using two ion exchange chromatographies (Fig ...
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bioRxiv - Biochemistry 2024Quote: The lysate was cleared by centrifugation at 12,000g for 30min at 10°C and the supernatant was applied onto a Talon Superflow beads batch (Merck). The immobilized proteins were washed with 50 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: Unless otherwise noted the standard assay was carried out at 65 °C for 10 min in 500 μL reaction mixture using 0.25 % (w/v) laminarin from Laminaria digitata (Merck) as substrate in 50 mM sodium phosphate buffer pH 6.0 (adjusted at assay temperature) ...
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bioRxiv - Microbiology 2023Quote: ... 100 ml of the cell suspension at an OD660 of 0.6 was harvested by centrifugation at 5,000×g for 10 min at 4°C and resuspended in 2 ml of BugBuster (Merck), and 50 μg/ml lysozyme and 1 mM phenylmethanesulfonyl fluoride (PMSF ...
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bioRxiv - Immunology 2021Quote: ... MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel (MCYTOMAG-70K, Merck) was used to measure GM-CSF ...
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bioRxiv - Neuroscience 2022Quote: ... MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel (MCYTOMAG-70K, Merck) was used to measure GM-CSF ...
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bioRxiv - Developmental Biology 2022Quote: ... immunofluorescence staining was performed using overnight incubation with polyclonal anti-Prosurfactant Protein C (ProSP-C) (Merck/Millipore/Sigma Aldrich, 1:500) followed by staining with polyclonal secondary antibody Goat anti-rabbit Alexa fluor 488 (Invitrogen,1:500) ...
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bioRxiv - Pathology 2023Quote: ... blocked with normal serum for 1 h at 22–24°C and incubated overnight at 4°C with rabbit polyclonal anti-Lubricin antibody (1:500, MABT401, MERCK, DEU). The sections were then washed three times with PBS ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Single product was validated on 1.5% agarose gel after incubated at 95 °C for two minutes in thermo cycler with hot lid then brought to 22 °C and mixed with formamide (Merck millipore) in 1:3 ratio ...
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DJ-1 (Park7) affects the gut microbiome, metabolites and development of Innate Lymphoid cells (ILCs)bioRxiv - Immunology 2019Quote: ... Membranes were then washed twice 5 min in TBST and incubated with the primary antibody over night at 4°C (human and mouse a-syn: 610786 BD Biosciences; GAPDH: #5174, Cell Signaling and GFAP #MAB360, Merck Millipore, Germany). After incubation with the first antibody ...
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Oxidative and non-oxidative active turnover of genomic methylcytosine in distinct pluripotent statesbioRxiv - Cell Biology 2020Quote: ... 10 μg/ml human holo-Transferrin (Merck), 12.5 mg/ml AlbuMAX I (Life Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... as described above. The pretreatments with translational inhibitors puromycin (f. c. 20 µg/ml) and cycloheximide (f. c. 100 µg/ml) (Merck, Darmstadt, Germany) were performed after harvesting of the cells in the import buffer for 30 min at 37°C.