Labshake search
Citations for Merck :
51 - 100 of 1380 citations for Recombinant Human CD40 protein Fc tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... recombinant SARS-COV-2 Spike protein was desalted and concentrated with C4 ZipTips (Merck Millipore, Darmstadt, Germany) and spotted on a ground steel target using 2’,5’-Dihydroxyacetophenone ...
-
bioRxiv - Developmental Biology 2021Quote: ... 500 μg/mL rice-derived recombinant human albumin and 213 μg/mL L-ascorbic acid 2-phosphate (both from Merck). After 24 h of CHIR99021 stimulation ...
-
bioRxiv - Biophysics 2021Quote: ... This mix included 98% unlabeled recombinant human E254N tubulin diluted in assay buffer containing oxygen scavengers (180 mg/ml catalase (C40, Merck) and 752 mg/ml glucose oxidase (22778.01 ...
-
bioRxiv - Biochemistry 2022Quote: ... The His6- tagged proteins were purified using Immobilised Metal Affinity Chromatography (IMAC) with 2mL Ni-NTA His•Bind® Resin (Merck), followed by incubation with TEV protease for 12 hours at 4 °C to remove the His6 tag ...
-
bioRxiv - Biochemistry 2019Quote: ... Fractions containing the desired His6-tagged protein were concentrated to 2 ml using Amicon® Ultra Centrifugal Filters (10,000 MWCO, Merck Millipore), and were directly injected into a size-exclusion chromatography column (Superdex 75 16/60 ...
-
bioRxiv - Cell Biology 2020Quote: Recombinant protein expression was performed using Eschericia coli strain Rosetta™ 2 (DE3) (Novagen) (Merck Cat. No 71403). Protein expression was always performed using freshly transformed chemically competent E ...
-
bioRxiv - Developmental Biology 2023Quote: ... healthy female cynomolgus monkeys were subjected to follicular stimulation by intramuscular injection of 20 IU of recombinant human follitropin alpha (rhFSH, Gonal F, Merck Serono) for 8 days ...
-
bioRxiv - Immunology 2019Quote: ... Fc IgG (AG714, Merck Millipore) at 2 μg/ml in 150 μl of PBS for one hour ...
-
bioRxiv - Biophysics 2019Quote: ... supplemented with 10 % FCS (Merck), 100 µg ml−1 penicillin (Life technologies) ...
-
bioRxiv - Biophysics 2019Quote: ... supplemented with 10% FCS (Merck), 100 µg ml−1 penicillin (Life technologies) ...
-
bioRxiv - Biophysics 2020Quote: ... supplemented with 10% FCS (MERCK), 100 μg ml−1 penicillin (Life technologies) ...
-
bioRxiv - Biochemistry 2021Quote: ... supplemented with 10% FCS (Merck) and 2 mM L-glutamine (Thermo Scientific ...
-
bioRxiv - Developmental Biology 2023Quote: ... + 10% FCS (Merck Millipore S0615) + 1% P/S (Thermo Fisher #15140122) ...
-
bioRxiv - Immunology 2024Quote: ... supplemented with 10% FCS (Merck), 2mM L-Glutamine (Gibco ...
-
bioRxiv - Cell Biology 2020Quote: Recombinant GST-mm-FMN2-eFSI (GST-FMN2-eFSI) proteins were expressed in Escherichia coli Rosetta bacterial cells (Merck Millipore). Bacteria were cultured in LB medium (100 mg/l ampicillin ...
-
bioRxiv - Immunology 2020Quote: ... 350 ng/mL active ERK2-GST tagged (Merck) or 175 ng/mL ERK1 plus 175 ng/mL ERK2-GST for 30 min at 30°C.
-
bioRxiv - Cell Biology 2022Quote: ... Recombinant BG4 (recBG4) (Millipore Merck) was added as control.
-
bioRxiv - Biochemistry 2020Quote: ... The recombinant protein was dialyzed overnight using an Amicon Ultra 15 mL spin dialysis column 10 000 MWCO (Merck, Germany). Purified protein was quantified using Bradford’s assay ...
-
bioRxiv - Neuroscience 2023Quote: ... the affinity resin was prepared by incubating 100 µg of recombinant protein with 20 µl of nickel beads (PureProteome Nickel magnetic beads, Merck) in 50 mM Pull-Down Buffer PDB (Tris–HCl pH 7.4 ...
-
bioRxiv - Biochemistry 2024Quote: ... Affinity-purified recombinant TrK13 proteins (WT, C580Y, R539T and C580Y) or commercially purchased BSA (A7030) or lysozyme (L6876) (Merck, Germany), each at 10 µM concentration ...
-
bioRxiv - Cell Biology 2020Quote: ... containing 10% FCS (Merck KGA, Germany), 4 mM glutamine (Merck KGA ...
-
bioRxiv - Plant Biology 2020Quote: ... The recombinant MBP fusion protein was concentrated with an Amicon Ultra-15 centrifugal filter unit (Ultracel 3K; Merck Millipore, Darmstadt, Germany) and used directly for electrophoretic mobility shift assay (EMSA).
-
bioRxiv - Physiology 2020Quote: ... tau/NTF or N-terminal (243-441) tau/CTF cDNA was used to produce recombinant tau protein in Escherichia coli BL21 (DE3) (Merck Millipore). Purification was performed as described previously (Hasegawa et al. ...
-
bioRxiv - Immunology 2020Quote: ... and 10% Fetal calf serum (FCS) (Merck). Both cell lines were cultivated in T75 culture flasks ...
-
bioRxiv - Bioengineering 2023Quote: ... the chips were coated with fibronectin (Human Plasma Fibronectin Purified Protein, Merck, Schiphol-Rijk, The Netherlands) in sterile PBS (100 μg/mL ...
-
bioRxiv - Molecular Biology 2020Quote: ... Fractions containing the respective recombinant proteins were pooled and concentrated using Amicon® Ultra-15 spin concentrators with 10 kDa cut-off (Merck Millipore). Concentrated protein pools were run on a Superdex 200 Increase 10/300 GL ...
-
bioRxiv - Biophysics 2019Quote: ... and 10 ml RPMI containing 0.75 % FCS (Merck) in a common 50-ml Eppendorf tube and heated up to 68°C using a common water bath ...
-
bioRxiv - Biochemistry 2021Quote: ... Fluorinated oil (FC- 40, Merck, Kenilworth, NJ, USA) was used to remove the excess amounts of the reaction solution and to seal the reactor (Supplementary Fig ...
-
bioRxiv - Bioengineering 2023Quote: ... FCS was mixed with 50% PEG-10,000 (Merck) at a 5:1 ratio and rotated at 4 °C for 3 h ...
-
bioRxiv - Immunology 2022Quote: ... mouse CC1 Anti-APC (Ab-7) (#OP80-100UG, Merck) and mouse anti-Olig2 (#66513-1-IG ...
-
bioRxiv - Immunology 2024Quote: ... or 10 µM of APC-366 (tryptase inhibitor, Merck) was employed to block human tryptase ...
-
bioRxiv - Cell Biology 2023Quote: Glass bottom well plates (Celvis) were coated with 5 µg/ml of human plasma fibronectin purified protein (Merck) for one hour at room temperature ...
-
bioRxiv - Cell Biology 2023Quote: Glass bottom well plates (Celvis) were coated with 5 µg/ml of human plasma fibronectin purified protein (Merck) for one hour at room temperature ...
-
bioRxiv - Pathology 2022Quote: ... recombinant anti-Cre (Merck Millipore, 69050, 1:1000) and revealed using Histofine reagent (Nichirei Biosciences) ...
-
bioRxiv - Neuroscience 2020Quote: ... mouse monoclonal anti-APC (1:250, #OP80, Merck, Darmstadt, Deutschland), rabbit polyclonal anti-MBP (1:200 ...
-
bioRxiv - Genomics 2020Quote: ... supplemented with 15% fetal calf serum (FCS) (Merck Millipore), 0.1mM b-mercaptoethanol (Sigma-Aldrich) ...
-
bioRxiv - Developmental Biology 2022Quote: ... was supplemented with 10% fetal calf serum (FCS) (Merck), 1x non-essential amino acids (NEAA ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... supplemented with 10% foetal calf serum (FCS, Merck, Germany). Once confluent ...
-
bioRxiv - Bioengineering 2022Quote: ... supplemented with 10% Fetal Calf Serum (FCS, #S0615, Merck), 4 mM L-glutamine (#25030-024 ...
-
REEP4 is recruited to the inner nuclear membrane by ELYS and promotes nuclear pore complex formationbioRxiv - Cell Biology 2020Quote: ... DNA was labeled with Hoechst 33342 (Merck). Coverslips were mounted with ProLong Diamond (ThermoFisher).
-
bioRxiv - Biophysics 2020Quote: ... was labeled with Biotin-NHS (Merck, 203112). Therefore 100 µg of 1g/l primary antibody were treated according to the labeling protocol above using 250 µg of Biotin-NHS ...
-
bioRxiv - Physiology 2019Quote: ... The hPCLS were treated with 10ng/ml recombinant human TGFß 1 protein (R&DSystems#240-B-010) and 25µM (in 750µl of DMEM) of GM6001 (Merck#CC1010). Lived dead staining was carried out using calcein-am/ ethidium homodimer kit (ThermoFisher#L3224 ...
-
bioRxiv - Cancer Biology 2021Quote: ... and 2 μg/ml of recombinant TNC (Merck Millipore) added to the medium ...
-
bioRxiv - Immunology 2021Quote: ... recombinant hepatitis B surface antigen (HBsAg) manufactured by Merck & Co. ...
-
bioRxiv - Biophysics 2020Quote: ... Recombinant DNA reagents and primers were purchased from Merck. As calibration samples in ALEX as well as anisotropy experiments ...
-
bioRxiv - Biochemistry 2023Quote: ... Active recombinant LKB1/STRADα/MO25α was purchased from Merck. Gateway pENTR plasmids encoding full length human BRSK1 & BRSK2 were generated as part of the NIH common fund initiative to Illuminate the Druggable Genome (IDG ...
-
bioRxiv - Cell Biology 2023Quote: ... and anti-mouse IgG (Fc) TRITC (Merck, #SAB3701020, 1:500).
-
bioRxiv - Molecular Biology 2021Quote: ... 6xHis-MPB-tagged 43GTPBP6 was expressed in E.coli BL21 (DE3) RIL cells (Merck Millipore) grown in LB media ...
-
bioRxiv - Neuroscience 2023Quote: ... or human post-mortem total protein/ synaptoneurosome samples were then mixed into equal volumes of 2X Laemmli buffer (Merck: S3401- 10VL) and boiled for 10 minutes at 98 °C ...
-
bioRxiv - Microbiology 2022Quote: ... and pre-incubated for 16 h with 1 µm aphidicolin (APC; Merck). Cells were pelleted (200 x g ...