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Citations for Merck :
201 - 250 of 521 citations for Recombinant Human CD3D Flag and Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2020Quote: ... anti-human MLKL (Merck Millipore, MABC604), phospho anti-human RPB1 S2 (Millipore ...
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α-catenin links integrin adhesions to F-actin to regulate ECM mechanosensing and rigidity-dependencebioRxiv - Cell Biology 2021Quote: ... Human plasma full-length fibronectin (Merck) was added to the dish at a final concentration of 10 μg/μl for a 1h incubation at 37°C ...
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bioRxiv - Immunology 2023Quote: Human IFNβ (IF014, Merck, KGaA, Darmstadt), IFNα2a (11100-1 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 5 μg/ml human insulin (Merck), and 50 μM hydrocortisone (Cayman ...
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bioRxiv - Immunology 2024Quote: ... Human MC lines HMC-1.1 (Merck) and HMC-1.2 (Merck ...
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bioRxiv - Cancer Biology 2023Quote: ... femurs and tibias in FACS buffer (PBS supplemented with 2% fetal calf serum (FCS; Merck) and 2 mM EDTA (Merck) ...
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bioRxiv - Cell Biology 2022Quote: ... The resulting supernatant was incubated with anti-FLAG M2 magnetic beads (M8823, Merck). After incubation for 12 hours at 4℃ ...
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bioRxiv - Cell Biology 2019Quote: ... Immunoprecipitation was performed using Anti-FLAG M2 affiinity gel (Sigma/Merck, Darmstadt, Germany) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Proteins were eluted from beads using 40 µg of 3x FLAG peptide (Merck) for 200µL of IP sample incubated on a rotator for 45 min at 4°C.
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bioRxiv - Molecular Biology 2021Quote: ... Crude protein extract was incubated with Anti-FLAG® M2 Magnetic Beads (Merck) overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... Antibodies used for immunoblotting were: α-FLAG M2 (1:500; Merck-Sigma, F3165), α-Matr3 (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... the supernatant was transferred onto equilibrated magnetic anti-FLAG M2 magnetic beads (Merck). Samples were rotated on a wheel for 1.75 h ...
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bioRxiv - Microbiology 2023Quote: ... The remaining 90% of lysate was incubated with anti-Flag antibody (Merck, F1804) coupled to Protein G Dynabeads (ThermoFisher ...
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bioRxiv - Cell Biology 2023Quote: ... and anti-Flag (IF: 1:500, cat#F7425, Merck Millipore, Burlington, MA, USA). Secondary antibodies were the following ...
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bioRxiv - Plant Biology 2024Quote: ... the blots were incubated with primary antibodies α-FLAG (Merck, F3165, 1:2000), α-GFP (Chromotek ...
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bioRxiv - Molecular Biology 2023Quote: ... and the immunopurified complexes were eluted with 3X FLAG peptide (Merck Millipore/Sigma). For tandem IP experiment ...
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bioRxiv - Microbiology 2023Quote: ... The remaining 90% of lysate underwent immunoprecipitation with anti-Flag antibody (Merck, F1804) coupled to Protein G Dynabeads (ThermoFisher ...
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bioRxiv - Molecular Biology 2019Quote: ... protocol with the administration of recombinant FSH (Gonal-F, Merck Serono, or Puregon, Merck Sharp & Dohme Corp. ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 μg purified recombinant LARK protein or 1 μg BG4 (MABE917, Merck, Darmstadt, Germany) was added ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were purified with His-Bind Resin and His-Bind Buffers (Merck Millipore) according to manufacturer’s protocol and stored at a concentration of 2mg/ml in elution buffer (Merck Millipore ...
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bioRxiv - Biophysics 2020Quote: ... the purified mEGFP-tagged NbALFA was concentrated to 1 mg/ml using an Amicon Ultra 30K filter (Merck Millipore) and stored at -80 °C before use ...
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Incidence of an intracellular multiplication niche amongst Acinetobacter baumannii clinical isolatesbioRxiv - Microbiology 2021Quote: A549 (human epithelial lung cell line) and EA.hy926 (human endothelial somatic cell line) cells were bought from Merck company and ATCC ...
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bioRxiv - Immunology 2020Quote: ... cells were resuspended at a concentration of 10×106/100μL in PBS containing 2% FCS (Merck). Staining for cell surface antigens was performed for 20 minutes at 4°C in the dark ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 ug inactive MEK1 (C-terminally His-tagged) in a total volume of 40 μL buffer (20 mM Hepes pH 8 (MERCK, Sigma Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... The His6-tagged proteins were purified using Immobilised Metal Affinity Chromatography (IMAC) with Ni-NTA His•Bind® Resin (Merck). Fractions containing the protein of interest were then dialysed against low-salt (50mM Tris pH8.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The His6-tagged proteins were purified using Immobilised Metal Affinity Chromatography (IMAC) with Ni-NTA His•Bind® Resin (Merck). Fractions containing the protein of interest were then dialysed against low-salt buffer (50mM Tris pH7.0 ...
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bioRxiv - Biochemistry 2023Quote: ... and transiently transfected with the plasmid DNA containing GFP-tagged protein of interest (See Table S7 for plasmid constructs) using GeneJuice transfection reagent (70967, Merck) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... His-tagged Trx1 was purified on a Ni2+ sepharose resin following the manufactureŕs instructions (cOmplete™ His-Tag Purification Resin, Merck).
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bioRxiv - Microbiology 2021Quote: ... and eluted in 150 µL wash buffer with 500 ng/µL FLAG peptide (Merck) by rotation with a rotator for 30 min at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was collected and incubated with anti-FLAG M2 magnetic beads (M8823, Merck) 4℃ overnight ...
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bioRxiv - Systems Biology 2021Quote: ... Proteins were detected using a monoclonal anti-FLAG M2 antibody (F3165, Sigma/Merck Millipore) and an HRP-conjugated polyclonal goat anti-mouse antibody (P0447 ...
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bioRxiv - Biochemistry 2021Quote: ... Supernatants were collected and incubated with beads conjugated to anti-FLAG M2 antibody (Merck). Beads were washed ...
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bioRxiv - Cancer Biology 2022Quote: ... extracts were incubated with 40 μL equilibrated ANTI-FLAG M2 Affinity Gel (Merck, A2220) for approximately 3 hours at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... and subsequently stained with primary antibodies: Mouse anti-FLAG (1:500) (monoclonal M2, Merck) or rabbit anti-myc (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... the following antibodies were used: rabbit anti-FLAG antibody (Merck Millipore cat. no. F7425), mouse anti-neurofilament 70 kDa antibody ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mg chromatin lysates were incubated with 50 µl FLAG M2 beads (Merck A2220) for 2 h at 4°C with rotation ...
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bioRxiv - Molecular Biology 2023Quote: ... extracts were incubated with 20 μL equilibrated anti-FLAG M2 Affinity Gel (Merck, A2220) for approximately 3 hours at 4 °C ...
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Oxidative and non-oxidative active turnover of genomic methylcytosine in distinct pluripotent statesbioRxiv - Cell Biology 2020Quote: ... 10 μg/ml human holo-Transferrin (Merck), 12.5 mg/ml AlbuMAX I (Life Technologies ...
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bioRxiv - Cell Biology 2019Quote: ... and human plasma full-length fibronectin (Merck) was added to the dish at a final concentration of 10 µg/µl for a 1h incubation at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... and human plasma full-length fibronectin (Merck) was added to the dish at a final concentration of 10 μg/μl for a 1h incubation at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... mouse anti-human α5 IgG1 (JBS5; Merck), mouse anti-human α5 IgG3 (P1D6 ...
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bioRxiv - Physiology 2022Quote: ... labelled human mitochondria primary antibody (Merck, MAB1273), 1:500 for 48 h at 4°C and washed with PBS overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... rat anti-human β1 IgG1 (AIIB2; Merck), mouse anti-human β1 IgG1 (Lia1/2 ...
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bioRxiv - Microbiology 2023Quote: ... mouse anti-human α5 IgG3 (P1D6; Merck), mouse IgG1 (JC1 ...
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Chromatin priming elements direct tissue-specific gene activity prior to hematopoietic specificationbioRxiv - Genomics 2023Quote: ... 180⍰µg/ml Human transferrin (Merck, T8158), 20% D4T Conditioned Media ...
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bioRxiv - Immunology 2023Quote: ... polyclonal anti-human PI3K p85 (Merck Millipore), anti-PI3 Kinase p110α (C73F8 ...
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bioRxiv - Cell Biology 2024Quote: ... or 5µg/cm2 human-plasma fibronectin (Merck) overnight in 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA tagged with 6xHis at the C-terminus was introduced into a pNG2 vector (a derivative of pET-3a, Merck-Novagen) linearized with NdeI and BamHI ...
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bioRxiv - Biochemistry 2022Quote: ... The His6- tagged proteins were purified using Immobilised Metal Affinity Chromatography (IMAC) with 2mL Ni-NTA His•Bind® Resin (Merck), followed by incubation with TEV protease for 12 hours at 4 °C to remove the His6 tag ...
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bioRxiv - Biochemistry 2019Quote: ... Fractions containing the desired His6-tagged protein were concentrated to 2 ml using Amicon® Ultra Centrifugal Filters (10,000 MWCO, Merck Millipore), and were directly injected into a size-exclusion chromatography column (Superdex 75 16/60 ...