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Citations for Merck :
651 - 700 of 1376 citations for Recombinant Human CD22 protein His tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... Protein lysate was prepared in Radio-immunoprecipitation assay (RIPA) buffer (Merck Millipore) with a protease inhibitor cocktail (Roche ...
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bioRxiv - Microbiology 2024Quote: ... the separated proteins were transferred onto Immobilon-P PVDF membrane (Merck Millipore). The membrane was blocked with 10% skim milk in Tris Buffered Saline ...
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bioRxiv - Cell Biology 2023Quote: ... lysates with equal protein concentration were incubated with Rabbit IgG (Merck, control) and 50 μg of Stwl antibody overnight at 4°C ...
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bioRxiv - Synthetic Biology 2024Quote: ... Aqueous solutions without protein components were filtered using Millex-GV filters (Merck), Novec HFE-7500 (3M ...
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bioRxiv - Developmental Biology 2024Quote: ... Following gel-electrophoresis proteins were transferred to a PVDF membrane (Merck, IPVH00010) using a semi-dry transfer cell (Biorad Transblot SD) ...
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bioRxiv - Physiology 2020Quote: ... Insulin concentration was determined using the MILLIPLEX® MAP Human Metabolic Hormone Magnetic Bead Panel (Merck KGaA, Darmstadt, Germany) according to the manufacturer s instructions.
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bioRxiv - Cell Biology 2021Quote: ... Purified human integrin α5β1 and anti-α5 (MAB1956Z) and anti-α5β1 (MAB1999) antibodies were from Merck (Kenilworth, NJ, USA). Volociximab was from Novus Biologicals (Littleton ...
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bioRxiv - Cell Biology 2021Quote: The digoxigenin-labeled antisense probe targeting bases 32-500 of human GNRH1 mRNA (NM_001083111.2) was transcribed in the presence of digoxigenin-11-UTP (Merck Millipore) in a reaction mixture containing linearized cDNA template (1 µg) ...
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bioRxiv - Biochemistry 2020Quote: ... was equilibrated with increasing concentrations of NAb (30 pM–1.0 µM) in the presence of 90% heat-inactivated human serum (H5667, Merck) at a temperature of 4 °C overnight ...
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bioRxiv - Biochemistry 2020Quote: ... was mixed with unlabeled SARS-CoV-2 spike S1 at increasing concentrations (100 pM–3.2 µM) in the presence of 90% heat-inactivated human serum (H5667, Merck) and incubated at 4 °C overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... CD3+ T cells were cultured in TexMACS Medium (Miltenyi Biotech) supplemented with 5% heat-inactivated human AB serum (Merck), 100 units/mL penicillin (Merck ...
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bioRxiv - Molecular Biology 2022Quote: ... and primers anchored in the homology arms of the HDRT specific for KI at the human TRAC locus (Merck). PCR amplicons (typically 2.5 mL ...
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bioRxiv - Cell Biology 2023Quote: 293T human embryo kidney (HEK293T) cells were grown in Dulbecco’s modified Eagle’s medium (4.5 g/l glucose) (Merck, Dorset, UK) enriched with 10% fetal bovine serum (FBS) ...
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bioRxiv - Biophysics 2021Quote: ... Proteins were transferred onto an Immobilon FL PVDF membrane (Merck Millipore, Darmstadt, Germany), probed with antibodies ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein concentration of each lysate was determined using a BCA assay (Merck, 71285).
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bioRxiv - Immunology 2021Quote: ... The deglycosylated protein was desalted and concentrated using Amicon filter (Merck Millipore, USA). The native protein and enzyme treated proteins were separated through SDS-PAGE and the proteins were transferred to the Nitrocellulose membrane using Trans-Blot Turbo Transfer System (Bio Rad) ...
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bioRxiv - Microbiology 2021Quote: ... The protein was concentrated using an Amicon Ultra 10 K device (Merck Millipore), loaded on a size exclusion column [ENrich SEC 650 10 x 300 Column (Bio-Rad)] ...
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bioRxiv - Microbiology 2021Quote: ... Purified protein was further concentrated using Amicon Ultra Centrifugal Filter Units (Merck Millipore).
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bioRxiv - Immunology 2021Quote: ... proteins were concentrated on a 0.5 mL 10 kDa filter (UFC501024, Merck-Millipore) by ultracentrifugation and purity was controlled by SDS-PAGE.
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bioRxiv - Plant Biology 2019Quote: ... All protein concentrations were determined using a Direct Detect® Infrared Spectrometer (Merck).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... proteins were electro-transferred to a polyvinylidene difluoride membrane (Merck Millipore, Darmstadt, Germany), which was incubated in the blocking buffer (5% non-fat milk ...
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bioRxiv - Plant Biology 2019Quote: ... using protein at 18 mg/ml (measured by Direct Detect® spectrometer (Merck)) ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoreactive proteins were visualized by Immobilion western chemiluminescence substrate (Merck-Millipore cat#WBKLS0500) [7].
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bioRxiv - Cancer Biology 2020Quote: ... The protein fractions were isolated and concentrated using 3kDa centrifugal filters (Merck Millipore). LC MS/MS was performed on the concentrated culture CMed and spectral analysis was performed ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Total proteins were spotted (10 μg) onto nitrocellulose membranes (Merck Millipore, Darmstadt, Germany) and left to dry ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Total proteins were spotted (10 μg) onto nitrocellulose membranes (Merck Millipore, Darmstadt, Germany) and left to dry ...
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bioRxiv - Immunology 2021Quote: 5 µl of 10 µm bead slurry (PureProteom Protein A magnetic beads, Merck) were used per reaction ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were concentrated to > 150 μM using centrifugal filters (Amicon Ultra; Merck Millipore), aliquoted and snap-frozen and stored at −80 °C ...
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bioRxiv - Neuroscience 2021Quote: ... proteins were transferred to a PVDF membrane (Immobilon-FL; Merck Millipore, Billerica, MA). Immunoblotting was performed with primary antibodies against Camk2a phosphorylated at pT286 (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were incubated with Protein A Sepharose beads (Merck, GE17-5280-01) for 1 h at 4°C ...
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bioRxiv - Plant Biology 2020Quote: ... All protein concentrations were determined using a Direct Detect® Infrared Spectrometer (Merck).
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bioRxiv - Microbiology 2020Quote: ... Proteins were detected by Immobilon Western Chemiluminescent HRP Substrate (Merck Millipore, MA, USA) and a LAS-3000 mini chemiluminescence imaging system (Fujifilm ...
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bioRxiv - Plant Biology 2021Quote: ... the hybridization with proper antibodies by SNAP i.d 2.0 Protein Detection System (Merck) was performed ...
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bioRxiv - Neuroscience 2021Quote: ... proteins were transferred onto low fluorescent Immobilon-FL membrane (0.45μm por size, Merck) using the Biorad wet-blot system (1.15h ...
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bioRxiv - Microbiology 2021Quote: ... Eluted protein was desalted using PD-10 desalting columns (Sigma-Merck, U.S.A/Germany). Concentration of the desalted protein was estimated using Bradford’s method and the protein was stored at -80 °C until needed.
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bioRxiv - Microbiology 2021Quote: ... and the bacterial pellets were resuspended in BugBuster protein extraction reagent (Merck, Germany). The cell suspensions were incubated on a rotary shaker for 20 minutes ...
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Observation of an α-synuclein liquid droplet state and its maturation into Lewy body-like assembliesbioRxiv - Molecular Biology 2020Quote: ... the protein was concentrated using Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore). A90C α-synuclein was labelled with 1.5-fold molar excess Alexa Fluor 488 C5 maleimide (Life Technologies ...
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bioRxiv - Molecular Biology 2022Quote: ... The resolved proteins were transferred onto a 0.45 m PVDF membrane (Immobilon, Merck) in ice-cold transfer buffer (25 mM Tris ...
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bioRxiv - Microbiology 2022Quote: ... The protein was concentrated using 10kDa Amicon filter (Merck Millipore, Burlington, MA, USA). The protein concentration in each fraction was estimated using the Bradford reagent (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... The purified proteins were then concentrated using AmiconTM Ultra 15 concentrators (MERCK Millipore) and stored in -80°C.
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bioRxiv - Cell Biology 2022Quote: ... Protein transfer to PVDF membranes (Immobilon-P, 0.45 µm pore size, IPVH00010, Merck) was achieved using a wet transfer system (Bio-Rad) ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentration was estimated using the Direct Detect® Infrared Spectrophotometer (Merck-Millipore). 100 µg protein where loaded on a gel to detect endogenous MDGA proteins in Western blots ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... at 1:5,000 through SNAP i.d.® 2.0 Protein Detection System (C73105, Merck). This apparatus has a vacuum-driven technology and a built-in flow distributor that actively drives reagents through the membrane.
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bioRxiv - Cancer Biology 2020Quote: ... 60 μL of Magna ChIP™ Protein A+G Magnetic Beads (Merck Millipore) was used in accordance with the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... and tyrphostin A47 (a tyrosine protein kinase [TPK] specific blocker; Merck, Darmstadt, Germany). For each antibody ...
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bioRxiv - Cancer Biology 2021Quote: ... denatured proteins were loaded onto 30 kDa amicon filter (Merck Millipore, Darmstadt, Germany). The buffer was exchanged with UA solution (8 M UREA in 0.1 M TEAB pH 8.5 ...
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bioRxiv - Biochemistry 2021Quote: ... imidazole was removed and the protein was concentrated using a Centricon (Merck Millipore) and preserved at −80 °C in small fractions at 2 mM concentration.
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bioRxiv - Neuroscience 2020Quote: ... Proteins separated in the gel were transferred to a PVDF membrane (Merck Millipore) using a submerged blotting apparatus and then visualized using Immobilon Western Chemiluminescent HRP Substrate (Millipore) ...
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bioRxiv - Immunology 2022Quote: ... The immunocomplex was captured by adding Protein G Agarose (Merck Millipore, Darmstadt, Germany). The protein binding to the beads was boiled in 2 x Laemmli sample buffer (Bio–Rad ...
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bioRxiv - Microbiology 2022Quote: ... Protein pellets were resuspended in 400 µl of 2.5% sodium deoxycholate (SDC; Merck) in 0.2 M EPPS-buffer (Merck) ...