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Citations for Merck :
201 - 250 of 357 citations for Recombinant Human CALR His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... sensitivity assays against human serum (Merck Millipore, Burlington, MA, USA), colistin (Sigma-Aldrich ...
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bioRxiv - Genomics 2023Quote: ... After incubation with FcBlock (PBS + 12% human serum AB (Merck)) for 10 min at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... The human cardiomyocyte cell line AC16 was purchased from Merck Millipore (Burlington ...
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... and HRP-conjugated goat anti-human IgG (AP309P, Merck KGaA). Protein detection was done with enhanced chemiluminescence (ECL ...
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bioRxiv - Cell Biology 2024Quote: ... coated with 50 µg/ml human serum fibronectin (Merck, Germany). Imaging was performed on a Nikon Widefield Ti2 equipped with a sCMOS ...
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bioRxiv - Cell Biology 2020Quote: Recombinant GST-mm-FMN2-eFSI (GST-FMN2-eFSI) proteins were expressed in Escherichia coli Rosetta bacterial cells (Merck Millipore). Bacteria were cultured in LB medium (100 mg/l ampicillin ...
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bioRxiv - Biophysics 2020Quote: ... beads were removed and recombinant dynein was concentrated in a 100 KDa cut-off filter (Amicon Ultracel, Merck-Millipore) to a final volume of 500 μL ...
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bioRxiv - Microbiology 2023Quote: ... were incubated for 2 h at room temperature with the respective recombinant glycosyltransferase enzymes (6.3 µg/ mL) and UDP-GlcNAc (2 mM, Merck) in glycosylation buffer (15 mM HEPES ...
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bioRxiv - Bioengineering 2021Quote: Three sender area squares (3 × 3 mm) were put into a 24-well containing 300 μL of anti-His antibody (Merck, Germany, NOVG70796-3) (10 μg mL−1 in DPBS ...
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bioRxiv - Biochemistry 2020Quote: ... The recombinant protein was dialyzed overnight using an Amicon Ultra 15 mL spin dialysis column 10 000 MWCO (Merck, Germany). Purified protein was quantified using Bradford’s assay ...
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bioRxiv - Cancer Biology 2021Quote: Primary HPMECs in EBM2 medium or ST1.6R cells in DMEM/1% FBS were cultured overnight and stimulated with 2 μg/ml of recombinant TNC (Merck Millipore) for 24 h ...
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bioRxiv - Neuroscience 2023Quote: ... the affinity resin was prepared by incubating 100 µg of recombinant protein with 20 µl of nickel beads (PureProteome Nickel magnetic beads, Merck) in 50 mM Pull-Down Buffer PDB (Tris–HCl pH 7.4 ...
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Appropriate glycemic management protects the germline but not uterine environment in type 1 diabetesbioRxiv - Developmental Biology 2024Quote: ... The dose of COS (75-400 IU daily of recombinant follicle stimulating hormone (Gonal-F Merck or hMG Menopur, Ferring) was individualized to patient’s age ...
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Appropriate glycemic management protects the germline but not uterine environment in type 1 diabetesbioRxiv - Developmental Biology 2024Quote: ... Ovarian follicle growth tracking was performed by scheduled transvaginal ultrasound examinations that lead to the planning timepoint for oocyte maturation triggered by administration of recombinant hCG (250 microgr SC Ovitrelle; Merck). Oocyte retrieval was carried out by transvaginal ultrasographically-guided follicular puncture 37 hours later.
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bioRxiv - Biochemistry 2024Quote: ... Affinity-purified recombinant TrK13 proteins (WT, C580Y, R539T and C580Y) or commercially purchased BSA (A7030) or lysozyme (L6876) (Merck, Germany), each at 10 µM concentration ...
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bioRxiv - Cell Biology 2021Quote: Human immortalised articular chondrocyte C28/I2 cells were purchased from Merck Millipore and cultured in DMEM/F12 (Gibco ...
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bioRxiv - Cell Biology 2022Quote: The human brain microvascular endothelial cell line hCMEC/D3 (Merck Millipore) was seeded in T25 flasks coated with rat tail type I collagen (Merck Millipore) ...
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bioRxiv - Cancer Biology 2020Quote: ... When stained for human nuclei (MAB1281, Merck Millipore, Fig. 4C-D), this was performed for 30 min at RT in the dark after EdU staining (Click-iT Assay ...
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bioRxiv - Microbiology 2020Quote: ... and affinity-purified rabbit polyclonal antibody against human CPSF6 (HPA039973; Merck) were used at a dilution of 1:1,000 ...
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bioRxiv - Neuroscience 2022Quote: The HCYTOMAG-60K-15 Human Cytokine/Chemokine Multiplex Assay (Merck Millipore) was employed to simultaneously analyze TNF-α ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 IU mL1 human chorionic gonadotropin (hCG; Chorulon; Merck Animal Health) and 1.0 mg mL1 oestradiol ...
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bioRxiv - Immunology 2023Quote: ... and human rIL-2 (30 U/ml) (Roche/Merck, Darmstadt, Germany) to the culture ...
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bioRxiv - Developmental Biology 2023Quote: ... Coating was performed with 20 µg/ml human fibronectin (Merck FC010) in PBS for at least 30’ ...
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Lowering mutant huntingtin by small molecules relieves Huntington’s disease symptoms and progressionbioRxiv - Molecular Biology 2023Quote: ... and anti-eIF4G1 for human samples (1:2000; 07-1800, Merck) or anti-eIF4G3 for mouse samples (1:5000 ...
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bioRxiv - Cancer Biology 2024Quote: ... and antibodies directed against anti-human mitochondria (1:100, Merck #1273;), CDK2AP1 (rabbit ...
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bioRxiv - Bioengineering 2024Quote: ... anti-human nuclei (HuNu; 1:100; MAB4383, Merck Millipore, MA, USA), anti-Ku80 (1:100 ...
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bioRxiv - Plant Biology 2020Quote: ... The recombinant MBP fusion protein was concentrated with an Amicon Ultra-15 centrifugal filter unit (Ultracel 3K; Merck Millipore, Darmstadt, Germany) and used directly for electrophoretic mobility shift assay (EMSA).
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bioRxiv - Physiology 2020Quote: ... tau/NTF or N-terminal (243-441) tau/CTF cDNA was used to produce recombinant tau protein in Escherichia coli BL21 (DE3) (Merck Millipore). Purification was performed as described previously (Hasegawa et al. ...
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bioRxiv - Biochemistry 2023Quote: ... and lysed in recombinant tau-lysis buffer (1 mM benzamidine, 1 mM PMSF, 1x cOmplete™, EDTA-free Protease Inhibitor Cocktail mix (Merck), 14 mM b-mercaptoethanol ...
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bioRxiv - Biochemistry 2020Quote: ... 500 μM ATP and ~0.5 μg commercially available human CyclinB-Cdk1 (Merck). The reaction was carried out at 30 °C and was stopped using SDS-PAGE sample buffer after 5 minutes.
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bioRxiv - Cell Biology 2019Quote: ... Human pancreatic organoids were additionally immuno-stained against Sox9 (primary antibody: Merck Millipore ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... a rabbit anti-human EGFR monoclonal antibody (EMD Millipore-MERCK, Temecula, USA) diluted 1:1000 or a rabbit anti-human VEGF-A polyclonal antibody (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... human tau constructs were expressed in the vector pNG2 (Merck-Novagen, Darmstadt) in E ...
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bioRxiv - Immunology 2021Quote: ... MILLIPLEX MAP Human High Sensitivity T Cell Panel (Merck Cat# HSTCMAG-28SK) were used ...
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Chromatin priming elements direct tissue-specific gene activity prior to hematopoietic specificationbioRxiv - Genomics 2023Quote: ... 501µg/ml Ascorbic acid and 180⍰µg/ml Human transferrin (Merck, T8158)) and then plated onto non-adherent dishes (ThermoFisher ...
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bioRxiv - Bioengineering 2023Quote: ... Immortalized human cerebral microvascular endothelial cell line (hCMEC/D3 Merck Life Science) were cultured on 250µg/ml collagen-I (Advanced Biomatrix ...
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bioRxiv - Developmental Biology 2023Quote: ... and reinjected after 48 hours 7.5IU hCG (human chorionic gonadotrophic hormone; Merck), before overnight mating with F1 or F1 hybrid transgenic mT/mG stud males (original mT/mG transgenic mouse line obtained from Jackson Laboratories ...
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bioRxiv - Biophysics 2023Quote: ... A solution was prepared by mixing 70 µL human plasma fibronectin (Merck), 20 µL green fibrinogen and 910 µL PBS ...
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bioRxiv - Microbiology 2024Quote: ... Human Serum Albumin with or without fatty acids were purchased from Merck. IVIg (Multigam® 5%) ...
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bioRxiv - Molecular Biology 2020Quote: ... Fractions containing the respective recombinant proteins were pooled and concentrated using Amicon® Ultra-15 spin concentrators with 10 kDa cut-off (Merck Millipore). Concentrated protein pools were run on a Superdex 200 Increase 10/300 GL ...
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bioRxiv - Immunology 2020Quote: ... urinary human menopausal gonadotropin (hMG) (HMG-lepori 75 UI, Angelini Farmacéutica, S.A, Spain) and recombinant hCG (Ovitrelle 250 micrograms, Merck Serono Europe Limited, Spain) or luprorelin acetate (Procrin 1 mg/0,2 ml ...
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bioRxiv - Neuroscience 2021Quote: ... and 10 ng/ml human leukaemia inhibitory factor (hLIF; Merck KGaA, Hesse, Germany) for 12 days ...
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bioRxiv - Immunology 2019Quote: ... anti-human C5aR or nonpeptide C5aR antagonist W-54011 (5 μM, Merck Millipore)(25 ...
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bioRxiv - Cancer Biology 2019Quote: ... Sections were then incubated with mouse anti-human mitochondria (clone 113-1, Merck) diluted in blocking solution at a dilution of 1:1000 at 4°C overnight ...
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bioRxiv - Neuroscience 2020Quote: Immortalized human cerebral microvascular endothelial cell line (hCMEC/D3) was purchased from Merck-Millipore (Darmstadt ...
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bioRxiv - Microbiology 2022Quote: ... microtiter plates coated with either human IgM (30 ng/ml) (Merck, No I8260) for the CP ...
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bioRxiv - Immunology 2020Quote: ... Rabbit anti-human CD11c (ab216655) underwent buffer exchange using 50kDa amicon filters (Merck) so as to place antibodies in 0.1M NaHCO3 ...
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bioRxiv - Neuroscience 2023Quote: ... The Luminex Milliplex® MAP human cytokine / chemokine magnetic bead panel (HSTCMAG28SK07; Merck) was used to detect cytokines ...
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bioRxiv - Microbiology 2024Quote: ... MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Customised 7-plex Panel (Merck Millipore) was used to quantify cytokine levels.
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bioRxiv - Bioengineering 2021Quote: ... the chemically decellularized aortas were treated with 900 units (25 μg/mL DNase I recombinant, RNase free, Cat. No. 4716728001, Roche, Merck KGaA, Darmstadt, Germany) using a total of 3 DNase treatment for 2 hours each to achieve the desired level of DNA assessed by (Quant-iT™ PicoGreen™ dsDNA Assay Kit ...