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Citations for Merck :
151 - 200 of 1259 citations for Recombinant Bovine CTLA4 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... in D-PBS supplemented with 1% bovine serum albumin (BSA, Merck, 05470). Primary antibodies diluted in blocking buffer were incubated with the cells at RT for 1 h ...
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bioRxiv - Molecular Biology 2022Quote: ... resuspended in 1X PBS with 0.05% Bovine Serum Albumin (BSA; Merck, 05479), and passed through a 35μm filter (Corning ...
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bioRxiv - Biophysics 2021Quote: ... and 10% (v/v) fetal bovine serum (Merck Millipore, Burlington, MA, USA). U-2 OS cells (ATCC ...
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bioRxiv - Physiology 2022Quote: ... SFM was replaced with SFM containing 2 % bovine serum albumin (BSA) (Merck). To stimulate the βARs ...
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bioRxiv - Microbiology 2022Quote: ... The membrane were blocked with 5% bovine serum albumin (Merck Millipore, USA) in TBST (1x Tris-Buffered Saline ...
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The activator domain of bacterial collagenases drives collagen recognition, unwinding and processingbioRxiv - Biochemistry 2023Quote: Two milligrams of insoluble fibrillar collagen from bovine Achilles tendon (Merck, Germany) were added to an Nanosep microcentrifugal device (0.2 µm pore size ...
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bioRxiv - Bioengineering 2023Quote: ... They were then incubated with a 3% bovine serum albumin (BSA, Merck)/ 0.1% Triton X-100 solution in PBS for 2 h at RT and subsequently overnight at 4 °C with primary or conjugated antibodies in a 1% BSA/ 0.1% Triton X-100 solution ...
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bioRxiv - Biophysics 2024Quote: ... supplemented with 10% fetal bovine serum (FBS, South America, Merck, Darmstadt, DE), 1% L-glutamine and 1% penicillin–streptomycin (Life Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... 0.05% TWEEN-20) supplemented with 200–400 U bovine DNase I (Merck) and 200 μL EDTA-free protease inhibitor cocktail (Merck ...
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bioRxiv - Neuroscience 2021Quote: ... Injections of 50 µl NGF at a concentration of 0.8 µM (NGF, human recombinant, Calbiochem, Merck, Germany) dissolved in phosphate buffer saline (PBS ...
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bioRxiv - Neuroscience 2020Quote: Recombinant GST-hSLP4A was expressed in and purified from BL21 Rosetta DE3 E.coli (Merck, Ca. No. 70954). 5ml of saturated pDEST15 hSlp4-a-transformed BL21 Rosetta DE3 E.coli culture were diluted in 500 ml 2x YT (Sigma Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... 1000 units/ml of recombinant Leukemia Inhibitory Factor (LIF, Interchim, #8V6280) and 0.1% β-mercaptoethanol (Merck, #M6250) on gelatin-coated plates ...
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bioRxiv - Developmental Biology 2023Quote: ... 400 µl of solution of recombinant TnC (Supplementary Materials and Methods) or purified human TnC (Merck Millipore) (15 µg/ml in 0.01% PBS-Tween ...
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bioRxiv - Cell Biology 2022Quote: ... Protein A and Protein G conjugated to HRP were purchased from Merck. DNA dye Hoechst 33342 was purchased from ThermoFisher Scientific and used at a final concentration of 5 μg/ml ...
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bioRxiv - Immunology 2020Quote: ... Thirty µl of Protein G (Protein G Sepharose Fast Flow, Merck, UK) slurry per sample was added in addition to 0.01% sodium azide and incubated on a roller overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Total protein concentration was quantified by means of BCA protein assay (Merck). 10-20 μg of protein was separated by size using a 4-12% Bis-Tris Plus gel ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Protein A and Protein G conjugated to HRP were purchased from Merck. DNA dye Hoechst 33342 was purchased from ThermoFisher Scientific and used at a final concentration of 5 µg/ml.
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bioRxiv - Bioengineering 2022Quote: ... Protein concentrations were determined with the BCA protein assay kit (Novagen, Merck KGaA), and protein homogeneity was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Cancer Biology 2021Quote: Intracellular protein was extracted from cells using the NucBuster Protein Extraction Kit (Merck) and quantified using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentration was estimated using BCA protein assay kit (Merck, 71285-3, Germany).
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bioRxiv - Neuroscience 2021Quote: ... protein concentration was quantified using a Sigma-Aldrich BCA protein assay kit (Merck). Subsequently ...
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bioRxiv - Immunology 2019Quote: ... Cells were interferon stimulated by the addition of 100 IU/ml recombinant interferon-β (IFN-β; Merck, 407318) to the growth media for 24 h ...
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bioRxiv - Zoology 2020Quote: ... recombinant RhATG5 and RhATG5191-199Δ were affinity-purified using Ni-NTA His•Bind Resin (Merck-Millipore, Darmstadt, Germany). Recombinant RhATG5 and RhATG5191-199Δ were then incubated with 10µg μ-calpain (Merck ...
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bioRxiv - Immunology 2022Quote: ... Animals were randomly assigned to be treated with either 170,000 IU/kg human recombinant interferon alpha-2a (Merck), or BSA/saline (0.9% NaCl ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then saturated with 5% Bovine Serum Albumin (BSA) (Merck, A4503 50G) in PBS 1X for 30 min ...
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bioRxiv - Microbiology 2022Quote: ... 0.05% TWEEN-20) was supplemented with 200–400 U bovine DNase I (Merck) and 200 μL EDTA-free protease inhibitor cocktail (Merck) ...
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bioRxiv - Microbiology 2022Quote: ... 0.05% TWEEN-20) was supplemented with 200–400 U bovine DNase I (Merck) and 200 μL EDTA-free protease inhibitor cocktail (Merck) ...
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bioRxiv - Biophysics 2022Quote: ... and blocking them with 5 w/v% bovine serum albumin (BSA, Merck KGaA), both diluted in PBS for 1 hour ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 5-10% fetal bovine serum (F7524; Sigma-Aldrich/Merck group, USA) and 1% penicillin/streptomycin (15140122 ...
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The activator domain of bacterial collagenases drives collagen recognition, unwinding and processingbioRxiv - Biochemistry 2023Quote: ... Type I collagen fibers from bovine Achilles tendon were purchased from Merck (Germany).
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bioRxiv - Physiology 2023Quote: ... The fixed cells were blocked and permeabilised with 1% bovine serum albumin (Merck) and 0.1% Triton™ X-100 (Merck ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 2 U mL−1 catalase from bovine liver (Merck-MilliPoreSigma, ref. C1345). NADH was added at a final concentration of 1 mM ...
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bioRxiv - Cell Biology 2023Quote: ... Nonspecific antibody blocking was for 1 h with 2% bovine serum albumin (Merck) in Tris-buffered saline ...
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bioRxiv - Physiology 2020Quote: ... Total proteins from the supernatant were quantified using a Bradford Protein Assay Kit (Merck) and subjected to electrophoresis on 15% SDS–PAGE gels ...
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bioRxiv - Physiology 2022Quote: ... Protein carbonylation was assessed with an Oxyblot protein oxidation detection kit (#S7150, Merck Millipore) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... 500 μg of protein were added to washed agarose protein A beads (Merck Millipore) and incubated on a rotating wheel for 1 h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... protein standards (Merck Sigma-Aldrich) specified in the Supplementary Material were separated on the same column.
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bioRxiv - Microbiology 2023Quote: ... protein A sepharose beads (Merck) were incubated with the cleared supernatant for an hour to remove non-specific binding ...
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bioRxiv - Neuroscience 2019Quote: ... the rats were treated with either 170,000 IU/kg IntronA (human recombinant interferon-alpha 2b, Merck Sharp & Dohme Limited), or saline (0.9% NaCl ...
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bioRxiv - Biophysics 2020Quote: ... beads were removed and recombinant dynein was concentrated in a 100 KDa cut-off filter (Amicon Ultracel, Merck-Millipore) to a final volume of 500 μL ...
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bioRxiv - Microbiology 2023Quote: ... were incubated for 2 h at room temperature with the respective recombinant glycosyltransferase enzymes (6.3 µg/ mL) and UDP-GlcNAc (2 mM, Merck) in glycosylation buffer (15 mM HEPES ...
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bioRxiv - Bioengineering 2021Quote: ... supplemented with 10% fetal bovine serum (FBS; Sigma-Aldrich/Merck, St. Louis, MO, USA) and penicillin (100 U/ml) ...
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bioRxiv - Microbiology 2021Quote: ... were diluted 1:500 in 4% bovine serum albumin (BSA) (Sigma-Aldrich, Merck, UK).
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bioRxiv - Cell Biology 2021Quote: ... both diluted in a solution of bovine serum albumin (BSA, Sigma-Aldrich, Merck, Germany) 3% w/v in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by 3 PBS washes and blocking with 5% bovine serum albumin (BSA; Merck) for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... containing 1.8 mM calcium and complemented with 10% fetal bovine serum (Merck, Darmstadt, Germany), 50 U/ml penicillin ...
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bioRxiv - Bioengineering 2023Quote: ... and 20 mg/mL fibrinogen (from bovine plasma, Merck, dissolved in DPBS and filtered) and mixed 1:1 with the cells to obtain a final solution of 10 mg/mL fibrinogen and 2 U/mL thrombin ...
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bioRxiv - Biophysics 2023Quote: ... and 0.05% (v/v) bovine bile (Sigma-Aldrich, Merck Life Science S.r.l., Milan, Italy) at 37°C in 10 mL screw-cap tubes (NuncTM ...
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bioRxiv - Microbiology 2023Quote: ... fixed cells were incubated with 1× PBS containing 1% bovine serum albumin (Merck KGaA) for 1 hour on ice ...
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bioRxiv - Bioengineering 2024Quote: ... Unfractionated Bovine Bile (CAS No – 8008-63-7, B3883-25G) was purchased from Merck Millipore GmbH ...