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Citations for Merck :
101 - 150 of 179 citations for Rat TMEM165 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... 400μL were mixed with 200μL of a 3 mg/mL collagen in 0.1% acetic acid solution (rat tail type I; Merck Millipore), followed by the addition of 10μL of 1 M NaOH ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Each group was then further split into two subgroups depending on whether the rats received bicuculline (Merck, Darmstadt, Germany) or vehicle (artificial cerebrospinal fluid ...
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bioRxiv - Biochemistry 2023Quote: ... plasmids were transformed into Rosetta (DE3) competent cells (Merck KGaA, Darmstadt, Germany) and inoculated onto LB agar plates supplemented with 50 µg/ml kanamycin and 34 µg/ml chloramphenicol ...
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bioRxiv - Microbiology 2023Quote: ... This plasmid carries the pRSF1030 replicon from RSFDuet™ (Novagen, Merck Millipore), araC and the araBAD promoter from pBAD/His (Invitrogen) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... while the rat abdominal aortic rings (15 mg wet mass/mL) were pre-incubated for 10 min in Medium 199 (Merck). The examined enzyme reaction started after pipetting the tracer substrate 1-14C-AA (3.7 kBq ...
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bioRxiv - Neuroscience 2020Quote: ... A single intravitreal injection of ML240 or EerI to all P23H rats previously anesthetized (ST and LT: Diethyl ether, Merck Millipore ...
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bioRxiv - Molecular Biology 2021Quote: ... Secondary antibodies fused to HRP were used for detection (Goat anti-mouse HRP 1:3000, BioRad; Goat anti-rat HRP 1:3000, Merck Millipore ...
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bioRxiv - Neuroscience 2022Quote: ... Six rats received an infusion of 1.5 μL (n = 4) or 3.0 μL (n = 2) of 10% glycerol (Merck, Darmstadt, Germany) in phosphate-buffered saline (PBS ...
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bioRxiv - Microbiology 2020Quote: ... The amplicon was cloned into BamHI and NdeI digested pET-28a(+) plasmid (Merck).
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bioRxiv - Molecular Biology 2023Quote: Cells were transfected with plasmid DNA using Gene Juice Transfection Reagent (Merck Millipore) 24 h after plating ...
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bioRxiv - Pathology 2023Quote: ... The resulting plasmid was transformed in Escherichia Coli BL21 (DE3) comnpetent cells (MERCK). Expression of TAT-Cre was induced growing transformed cells in LB Overnight Express™ Instant medium (Novagen ...
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bioRxiv - Physiology 2020Quote: ... according to the manufacturer’s instructions (Mouse Leptin ELISA Kit, 90030; Crystal Chem, Zaandam, Netherlands; Rat/Mouse Insulin ELISA Kit, cat. EZRMI-13K; Merck Millipore). The intra- and interassay coefficients of variation (CVs ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were transferred to well-plates for exposure to two primary antibodies (RFP antibody 5F8 rat for mCherry, RRID: AB_2336064, Chromotek, 1:1000; and mouse-monoclonal anti-CaMKIIα, RRID: AB_309787, Merck Millipore, 1:300) which were added to 0.1 M PBS-Tx 0.2% containing 5% normal goat serum ...
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bioRxiv - Biophysics 2021Quote: Cultures of dissociated rat hippocampal primary neurons were prepared from postnatal P0-P2 Wistar rats of either sex and cultured on glass coverslips coated with 100 µg/mL poly-ornithine (Merck KGaA) and 1 µg /mL laminin (BD Biosciences) ...
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bioRxiv - Neuroscience 2021Quote: Immunohistochemical staining was performed on free-floating brain sections using the following primary antibodies: mouse anti-rat TH (1:4000, MAB318, Merck Millipore), rabbit anti-Girk2 (1:500 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: The chemoreflex was activated by systemic bolus intravenous injections of 0.1 mL of KCN (40 μg per rat; Merck, Darmstadt, Germany) following procedures described by Franchini & Krieger (1993 ...
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bioRxiv - Immunology 2021Quote: ... Freshly isolated cells (4 x 106) were seeded in a pre-prepared gel matrix containing 1 mg/mL rat tail collagen type I (Merck Millipore) in DMEM ...
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bioRxiv - Microbiology 2022Quote: ... Cytokine and chemokine levels were measured with a commercial rat cytokine/chemokine magnetic bead panel 96-well plate assay kit (Milliplex MAP kit, Merck Millipore), which detects 5 cytokines and chemokines including IP-10/CXCL10 ...
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bioRxiv - Neuroscience 2023Quote: ... brain hemispheres were dissected from postnatal Sprague Dawley rats (P0-P2) and dissociated with 0.25% trypsin (Sigma Aldrich, Merck, Darmstadt, Germany) and 0.004% DNAse (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: Astrocytes for long-term neuronal cultures were prepared by dissociating newborn rat cortices with 2.5% trypsin and 1 mg/ml DNase (Merck, cat# 10104159001) and plating the cells in MEM with L-glutamine supplemented with 0.6% glucose ...
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bioRxiv - Neuroscience 2023Quote: ... rats were anesthetized and underwent transcardial perfusion with 100 mL of saline followed by 100 mL of 4% paraformaldehyde (Merck, Germany) at 72 h following ICH induction ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and follicle stimulating hormone (FSH) were determined using MILLIPLEX MAP Rat Pituitary Magnetic Bead Panel following manufacturer’s protocol (Merck-Millipore, Burlington, MA). Serum free fatty acids were measured using kits from Cell Biolabs ...
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bioRxiv - Biophysics 2019Quote: The DNA sequence encoding for the ARD of rat TRPV1 (residues 100-262) was cloned into a pET28c vector (Merck-Millipore, USA). For expression ...
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bioRxiv - Neuroscience 2020Quote: ... glial glutamate transporter GLT1 (guinea pig, polyclonal, synthetic peptide from the C-terminus of rat GLT1, Merck, AB1783, AB_90949, dilution 1:1,000), GFP (chicken ...
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bioRxiv - Microbiology 2020Quote: ... and probed with 1:1000 diluted primary anti-Ago2 rat monoclonal antibody clone 11A9 (Millipore Sigma/ Merck KGaA MABE 253, Darmstadt, Germany), 1:25000 diluted primary mouse monoclonal anti-beta actin antibody (AC-15 ...
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bioRxiv - Cell Biology 2023Quote: ... The protein concentration of the cell lysates was measured using the BCA assay and insulin levels were quantified by radioimmunoassay (rat insulin RIA kit, Merck, Darmstadt, Germany).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... rat slices were exposed to 3 μM dihydroethidium (DHE) while in humans’ sections it was added 4 μM DHE (Merck, Darmstadt, Germany) for 30 minutes at 37ºC 51 ...
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bioRxiv - Cell Biology 2020Quote: ... pDEST15 plasmids were transformed in Rosetta™ 2(DE3)pLysS Competent bacteria (Merck Millipore). Bacteria cultures were grown with the proper antibiotic at 37°C until an OD of 0.6 was obtained ...
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bioRxiv - Cell Biology 2024Quote: ... The cloned plasmids were transfected into HEK293T cells using GeneJuice transfecting agent (Merck Millipore). Forty-eight hours after transfection ...
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bioRxiv - Microbiology 2019Quote: ... were maintained at 37°C on rat collagen diluted at 100µg/mL in water (Cultrex; catalog no. 3443-100-01) in EndoGro medium (Merck Millipore; catalog no. SCME004) supplemented with 5% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were subsequently washed with PBS and incubated for 1 h with the proper secondary antibodies (1:400, goat anti-rat Alexa-Fluor® 568, ab175476, abcam; 1:400, goat anti-mouse Alexa-Fluor® 488, AP124JA4, Merck Millipore). For nuclear counterstaining slices were incubated with DAPI (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... The sequence was cloned into the pET42a plasmid (Cat No 70561, Novagen, Merck, Overijse, Belgium) in frame with an N-terminal caspase3-cleavable glutathione S-transferase (GST)-tag (26) ...
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bioRxiv - Cancer Biology 2022Quote: ... with lentiviral particles carrying single CRISPR guide RNA (Custom CRISPR gRNA Plasmid DNA, Merck, USA) directed to DCTD eRNA ...
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bioRxiv - Cancer Biology 2024Quote: ... and the RDF plasmid encoding the RD114 envelope) using the GeneJuice transfection reagent (Merck Millipore). Viral supernatant was collected 48 and 72 hours after transfection ...
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bioRxiv - Neuroscience 2020Quote: ... rat polyclonal anti-Vangl2 (clone 2G4, 1:200 for Western blotting, 1:400 for immunoprecipitation, 1:500 for immunostaining, Merck Millipore, catalog no. MABN750), mouse monoclonal IgG1k anti-GFP (clones 7.1 and 13.1 ...
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bioRxiv - Cell Biology 2020Quote: ... pDEST42-Ctdnep1_C-ter plasmid was transformed in Rosetta™ 2(DE3)pLysS Competent bacteria (Merck Millipore), Bacteria cultures (500 ml ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmid DNA was isolated from the transformant and transformed into Rosetta (DE3) cells (Merck Millipore, USA). For purification ...
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bioRxiv - Biochemistry 2022Quote: ... The plasmid was used to transform Escherichia coli BL21 Rosetta2 (DE3) expression strain (Merck, Darmstadt, Germany), grown to exponential phase in 1 L lysogeny broth (LB ...
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bioRxiv - Cell Biology 2024Quote: ... The full-length MDM2 coding sequence was isolated from Addgene plasmid #16233 (pcDNA3 FRT MDM2) by PCR using the KOD hot start polymerase kit (Merck #71086), using primers that included a 5’ BamHI restriction site ...
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bioRxiv - Neuroscience 2020Quote: ... Cells (primary culture of rat ENS) and tissues from mice (hippocampus, ileum, proximal and distal colon) were lysed in RIPA lysis buffer (Merck Millipore, Fontenay sous Bois, France) containing phosphatase inhibitor cocktail II (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... Transfection of plasmids in MEF and HEK293T was carried out using GeneJuice Transfection Reagent (Merck Millipore, 70967) following the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2020Quote: ... Table S3) and subcloned it into the NdeI/XhoI sites of a pET-22b(+) plasmid (Merck Millipore) so that its expression is driven by an IPTG-inducible T7 promoter ...
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bioRxiv - Developmental Biology 2019Quote: ... were transformed with the pET-UBE2D3 plasmids and protein expression was induced with 0.4 mM IPTG (Merck) for 3h at 37°C ...
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Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: ... plasmid coding for 6x-His-Cas9 were transformed in Rosetta DE3 Novagen competent cells (Merck Millipore, USA). Protein production was induced using autoinduction medium (Formedium ...
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bioRxiv - Molecular Biology 2023Quote: ... 2958 bp) was purified using commercial DNA purification kits (GenElute™ HP Plasmid Miniprep kit, Merck, #NA0160), according to standard procedures ...
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bioRxiv - Microbiology 2023Quote: ... coli RIL strains carrying the appropriate plasmid were grown in 1 l Luria-broth (LB) (Merck Millipore) supplemented with kanamycin (100 μg/ml ...
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bioRxiv - Genetics 2022Quote: ... Alendronate 1.0 ug/kg body weight was subcutaneously injected into rats in ALN group daily (alendronate sodium pure dry, provided by Merck and Co., Inc., Rahway, NJ, USA) (Diab ...
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bioRxiv - Cell Biology 2021Quote: ... Transfections of DNA plasmids were carried out with the NanoJuice Transfection Kit (71900-3, Merck, Kenilworth, NJ, USA) or the Avalanche-Everyday Transfection Reagent (EZT-EVDY-1 ...
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bioRxiv - Molecular Biology 2021Quote: ... Taq pol and StuI genes were subcloned for expression in the pET24-d plasmid (Novagen, Merck Millipore, Germany) using its NcoI and XhoI restriction sites ...
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bioRxiv - Microbiology 2022Quote: ... Cells were transfected by mixing 2 µg of DNA (split evenly by mass between the plasmids indicated) and 1.5 μg of branched polyethylenimine (PEI; average MW ∼25,000, Merck) in Opti-MEM (ThermoFisher) ...