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Citations for Merck :
201 - 231 of 231 citations for Rat IgG SAP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... followed by incubation with a 1:5000 dilution of a donkey anti-rabbit IgG HRP antibody (AP182P, Merck Millipore) and 1:5000 dilution of mouse anti-GAPDH (ab8245 ...
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bioRxiv - Neuroscience 2020Quote: ... Cells (primary culture of rat ENS) and tissues from mice (hippocampus, ileum, proximal and distal colon) were lysed in RIPA lysis buffer (Merck Millipore, Fontenay sous Bois, France) containing phosphatase inhibitor cocktail II (Roche ...
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bioRxiv - Developmental Biology 2021Quote: ... and incubated with FITC (Fluorescein isothiocyanate) conjugated secondary antibody (0.5 μg/ml of Goat Anti-Mouse IgG-FITC in 5%BSA) (GeNei, Merck, USA) (Cat.no ...
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bioRxiv - Cancer Biology 2022Quote: ... Primary antibodies were detected with HRP-conjugated anti-rabbit or anti-mouse IgG and visualised with Immobilon Western HRP substrate (Merck).
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibodies, anti-rabbit IgG-HRP (1:5000, #7074, CST) or anti-mouse-HRP (1:10000, #12-349, Merck Millipore) in TBST containing 5% milk for 1 h at RT ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary Anti-rabbit IgG-HRP Cell Signalling/70745, London, UK), HIF2A (Novusbio/NB100-122, Secondary Anti-rabbit IgG-HRP Cell Signalling/70745, London, UK) and GAPDH (Merck/MAB374 ...
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bioRxiv - Microbiology 2022Quote: ... the Milliplex SARS-CoV-2 Antigen Panel 1 IgG was used according to the manufacturer’s instructions (Merck Millipore, Darmstadt, Germany).
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bioRxiv - Cancer Biology 2019Quote: ... Secondary antibody incubation was performed at room temperature for 90 min utilizing a horseradish-peroxidase (HRP)-labeled anti-rabbit IgG (Merck), and staining was visualized with DAB (Dako ...
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bioRxiv - Molecular Biology 2019Quote: ... followed by washing and staining with horseradish peroxidase-conjugated donkey anti-rabbit or anti-mouse IgG secondary antibodies (1:5,000 dilution; Merck Millipore), respectively ...
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bioRxiv - Biochemistry 2019Quote: ... ∼500-ml cell suspension containing the secreted IgG was clarified by centrifugation and subsequent filtration through a 0.45 µm HAWP membrane (Merck Millipore). The filtrate was mixed with an equal volume of binding buffer (20 mM Na2HPO4/NaH2PO4 ...
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bioRxiv - Neuroscience 2020Quote: ... followed by 2h incubation with Alexa Fluor-488 goat anti-rabbit IgG secondary antibody (1:500, A11008, Merck Millipore, MA). Slices were transferred to glass slides and covered with Fluoromount (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... 500ng/ml purified total IgG stimulation was added the next day for 4 hrs followed by an incubation of 30 min with ATP (Merck). Culture supernatants were collected for further cytokine analysis and cells lysates were prepared for qPCR analysis ...
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bioRxiv - Cell Biology 2023Quote: ... Total IgG were isolated from post-immune sera by affinity purification on Sepharose-protein G (Merck, Cytiva 17-0618-05). Custom sheep pAb anti-pS55 and anti-pS44 NDC80 pAb was raised by the Scottish Blood Transfusion Service using a 4-month long program ...
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bioRxiv - Neuroscience 2023Quote: ... this was followed by the incubation in the blocking solution containing secondary antibody anti-Rabbit or/and goat IgG antibody conjugated with HRP (GENA934, AP106P, Merck) at room temperature for 1 hour for enhanced chemiluminescence ...
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bioRxiv - Evolutionary Biology 2023Quote: ... CDK9 was immunoprecipitated with 15μg of an anti-CDK9 antibody (D-7, sc-13130, lot no # B1422) or control mouse IgG (12-371, Merck) in IP Buffer with 2X SDS buffer (100mM NaCl ...
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bioRxiv - Genetics 2022Quote: ... Alendronate 1.0 ug/kg body weight was subcutaneously injected into rats in ALN group daily (alendronate sodium pure dry, provided by Merck and Co., Inc., Rahway, NJ, USA) (Diab ...
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bioRxiv - Biophysics 2021Quote: ... The antibody was revealed using Atto 594 goat anti-mouse IgG (1:500, 76085-1ML-F, Merck & Co., Kenilworth, NJ, USA). The coverslips were rinsed in PBS ...
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bioRxiv - Biochemistry 2021Quote: ... After washing, the bound nanobody was detected by HRP-conjugated Goat anti-Alpaca IgG (Cat. S001P, NBbiolab) using Tetramethylbenzidine (TMB) (Merck, Cat.T2885) as a substrate for HRP ...
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bioRxiv - Neuroscience 2022Quote: ... and 3% BSA followed by overnight incubation at room temperature with rabbit anti-KCC2 IgG (1:1000 dilution; #07-432, Merck-Millipore) (Yassin et al. ...
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bioRxiv - Microbiology 2023Quote: ... The secondary antibodies used for IB were goat anti-rabbit (Cat # AP307P) and goat anti-mouse (Cat # AP308P) IgG HRP-conjugated antibodies (Merck Millipore).
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bioRxiv - Bioengineering 2023Quote: ... samples were washed in PBS for 3 times and Alexa Fluor 555 conjugated secondary goat anti-mouse IgG antibody (1:100; Merck, Germany) was added for immunostaining in the dark for 2 hours at RT ...
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bioRxiv - Cell Biology 2023Quote: ... specimens were washed 3x with PBS before incubation in a solution of anti-Rabbit-IgG-ATTO 594 (Merck, #77671-1ML-F) as secondary antibody ...
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bioRxiv - Neuroscience 2020Quote: ... Blots were washed and probed with secondary antibody for one hour at RT (anti-mouse IgG, horseradish peroxidase linked; 1:5,000; Merck, Burlington, MA, USA). Bands were visualized using high sensitivity Pierce ECL Plus (Thermo Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... β-actin which was used as loading control was detected using a rabbit polyclonal IgG against mouse β-actin (A2103, Merck, Darmstadt, GER) diluted 1000-fold ...
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bioRxiv - Neuroscience 2021Quote: ... Secondary antibodies (as appropriate) were added for 1 hour at room temperature (goat anti-rabbit/ goat anti-mouse, IgG, HRP conjugated; Merck Millipore (1:5000)) before visualisation using enhanced chemiluminescence (1.25 mM luminol ...
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bioRxiv - Neuroscience 2019Quote: ... cortical sections were incubated overnight at 4 °C with anti-glial fibrillary acidic protein (GFAP, 1:1000, chicken IgG, Merck Millipore, Burlington, EUA), anti-Iba1 (1:200 ...
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bioRxiv - Cell Biology 2019Quote: ... anti-rabbit IgG (goat, coupled to Alexa 488, Thermo Fisher, A32731, 1:500 and donkey, coupled to Cy3, Merck Millipore, AP182C, 1:1000), anti-goat IgG (donkey ...
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bioRxiv - Neuroscience 2021Quote: ... unbound primary antibodies were removed by washing in TBST and the blots were incubated with HRP-conjugated secondary antibodies for 1 hour at room temperature: goat anti-mouse IgG and IgM HRP conjugated antibodies (1:4000, Merck EMD Millipore, Ap130P), or goat anti-rabbit IgG antibody ...
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bioRxiv - Physiology 2023Quote: ... they were incubated in their respective secondary antibodies for 1 h at room temperature (goat anti-rabbit, IgG, HRP conjugated; Merck Millipore (1: 5000) in 5% milk-TBST ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The IgG-containing fractions were combined and buffer-exchanged using a Merck Amicon™ Ultra centrifugal filter tube (Merck™, cat no. 10581342) in sterile PBS ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining sample was incubated o/n on a rotary shaker at 4 °C with 2 μg of the appropriate antibody (IgG, 12-370 Merck; pSTAT1, 7649 Cell Signaling). The next day 25 μl Dynabeads resuspended in CDB/c were added and incubated for at least 1 h at 4 °C on a rotary shaker ...