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Citations for Merck :
151 - 179 of 179 citations for Rat FAM24A shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... cells were transfected with plasmids encoding the respective heterologous proteins using the Genejuice transfection reagent (Merck, Darmstadt, Germany). After 48 hours of incubation at 37°C at 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... after 42°C heat shock transformation and extracted using the GenElute HP Plasmid Miniprep Kit (Sigma-Aldrich, Merck). To generate pARL-PfCen1-GFP ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA-calcium phosphate transfection complex was prepared by gently adding plasmid DNA and 2M CaCl2 (Sigma-Aldrich; Merck KGaA) dropwise to HBS buffer (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... and/or with plasmid DNA (e.g. 1µg/well for 6-well plates) for 24 hrs using GeneJuice (Merck Millipore). siRNAs targeting USP7 ...
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bioRxiv - Cancer Biology 2022Quote: Lentiviral supernatants were generated into HEK293T cells by transient co-transfections of lentiviral CAS9-RFP Lenti Plasmid (Merck, USA) construct and appropriate amount of packaging vectors (Mission Lentiviral Packaging Mix ...
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bioRxiv - Cell Biology 2023Quote: ... HCT116 wild-type or p53 missense mutant cells were transfected with the plasmids using X-treme GENE HP (Merck) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... HEK-293T and HuTu-80 cells were transfected with the pSpCas9-GFP-LRP10gRNA plasmid using GeneJuice® reagent (Merck) according to manufacturer’s specifications ...
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bioRxiv - Neuroscience 2023Quote: ... HEK-293T and HuTu-80 cells were transfected with the pSpCas9-GFP-LRP10gRNA plasmid using GeneJuice® reagent (Merck). After 48 h ...
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bioRxiv - Biophysics 2021Quote: ... 250 ng of each plasmid encoding VN and VC were transfected using 4 μL of 1 μg/mL PEI (Merck) for each μg of DNA ...
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bioRxiv - Molecular Biology 2020Quote: ... Three individual clones were picked and amplified in LB broth including antibiotics for 12 h at 37°C and isolated using GenElute– HP Plasmid MaxiPrep Kit (Merck). Integrity of purified expression constructs was validated by gel electrophoresis and the concentration was determined by absorbance measurement ...
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bioRxiv - Biochemistry 2021Quote: ... Transient transfection of U2OS and HepG2 cells with plasmid DNA was carried out using GeneJuice (Merck Millipore, cat. number 70967) according to manufacturer’s instruction and keeping GeneJuice to DNA ratio of 3:1 (volume in μl ...
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bioRxiv - Genetics 2022Quote: ... Transient transfection was carried out in HEK293T cells transfected with 1 μg of plasmid DNA in the presence of X-tremeGene9 DNA transfection reagent (#6365809001, Merck), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Transfection with plasmids carrying the coding sequences of the indicated proteins was performed using Genejuice transfection reagent (Merck, Darmstadt, Germany). 48 hours after transfection ...
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bioRxiv - Cell Biology 2024Quote: ... Truncated forms were generated by PCR of the entire plasmid except the region to be excluded using KOD HotStart DNA polymerase (Merck) and the forward primers pUL71 295 fwd ...
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bioRxiv - Cell Biology 2024Quote: ... HEK293 were seeded at 500,000 cells/mL concentration in 25 cm2 flasks and transfected after 24h with plasmid encoding nirFAST-P2A-EGFP using GeneJuice (Merck) according to manufacturer’s guidelines ...
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bioRxiv - Biochemistry 2021Quote: ... These Wild Type or Mutant Type let-7a-5p plasmids were co□transfected into treated human chondrocytes cells along with NC mimics or KCNQ1OT1 mimic (Sigma□Aldrich; Merck KGaA) using Lipofectamine 6000 following manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... PfCERLI1HAGlmS parasites were transfected with the an episomal cytosolic GFP expressing plasmid pHGBrHrBl-1/2 and maintenance of this plasmid was selected for using 5 μg/mL blasticidin-S-deaminase HCl (Merck Millipore). Maintenance of the SLI-TGD plasmid was selected for using 20 μM WR99210 ...
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bioRxiv - Genomics 2021Quote: Localization of resulting plasmids was assessed with immunofluorescence staining using an anti-flag antibody (F7425, Sigma Aldrich, Merck, Kenilworth, NJ, USA) in combination with Alexa 546 secondary antibody (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... cells were transfected with 1-2 µg of pcDNA5 FRT/TO plasmids containing the gene of interest using GeneJuice transfection reagent (Cat#70967, Merck Millipore) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... The pNGS2-N12-BC libraries were prepared by purification from the harvested cells by using either GenElute Plasmid Miniprep (Merck, Germany) or NucleoBond Xtra Midi (TaKaRa Bio Inc. ...
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bioRxiv - Microbiology 2019Quote: ... recodonised sequence was synthesised (gBlock gene fragments, Integrated DNA Technologies) and Gibson cloned (Gibson et al., 2010) into BamHI and NdeI digested pET-28a(+) plasmid (Merck, Darmstadt, Germany). This allowed for expression of an N-terminal 6xHis tagged shortCAP recombinant protein in Escherichia coli BL21 cells under the control of T7 lac promoter ...
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bioRxiv - Immunology 2022Quote: ... coli transformed with plasmid pET42 expressing N-terminally GST- and HIS-tagged human IL-18 were grown at 37°C in Lysogeny broth (LB) medium containing kanamycin (25 μg/ml) as selection marker for the pET42a plasmid (Cat No 70561, Novagen, Merck, Overijse, Belgium). When the optical density at 600 nm (OD600 ...
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bioRxiv - Biophysics 2022Quote: ... an amber codon (TAG) was introduced using appropriate mutagenic oligonucleotides and a protocol for whole plasmid site-directed mutagenesis employing KOD polymerase (Merck Millipore, Germany) as detailed in manufacturer’s instructions and previous work (Zheng et al. ...
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bioRxiv - Microbiology 2024Quote: ... HEK293T cells transfected with Flag-tagged NSP1 or vector plasmid were treated with the following compounds for 6 h: 10 μM of MG132 (Merck, Cat# 474787), 50 μM of chloroquine (CQ ...
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bioRxiv - Genomics 2021Quote: ... These pX330-mC plasmid DNAs and donor DNA plasmid were isolated using FastGene Plasmid Mini kit (Nippon Genetics, Tokyo, Japan) and filtered using MILLEX-GV® 0.22 μm filter unit (Merck Millipore, Darmstadt, Germany) for microinjection ...
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bioRxiv - Cancer Biology 2022Quote: ... 293T cells were co-transfected with second generation LV packaging plasmids pMD2.G and pCMV-dR8.74 (plasmidFactory, Bielefeld, Germany) and CAR transfer vectors using GeneJuice® Transfection Reagent (Merck-KGaA, Darmstadt, Germany) according to manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2023Quote: ... 250 ng of pTOPflash (wt-TCF) or pFOPflash (mutated-TCF binding site, negative control) reporter plasmids (TOPflash luciferase reporter assay, Merck Millipore, Darmstadt, Germany) were transfected with Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... were generated by PCR and cloned into the pFlag-CMV4 plasmid (for N-terminal Flag fusion in mammalian cells, cat#E7158, Merck Millipore, Burlington, MA, USA). CDS of ARVA ...
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bioRxiv - Cell Biology 2023Quote: ... to validate the gene expression the plasmid (vide-supra) was transfected into HEK293 cells using PEI (Sigma-Aldrich/Merck Cat. No. 49553-93-7) following an optimized version of the standard protocol(Rajendra ...