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Citations for Merck :
251 - 300 of 4134 citations for Rat Eukaryotic Translation Initiation Factor 4E Binding Protein 1 EIF4EBP1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... rinsed with PBS and the proteins were visualized with Immobilon Western Chemiluminiscent HRP substrate (Merck/Millipore, used at 1:1:3 (H2O) dilution ...
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bioRxiv - Physiology 2021Quote: ... For colocalization 1:100 anti-NBCe1 was incubated with 1:1,000 anti-Clara Cell Secretory Protein (CCSP; Merck Millipore cat#07-623) or 1:200 anti-alpha tubulin (Santa Cruz cat#sc-5286 ...
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bioRxiv - Physiology 2024Quote: ... For colocalization 1:100 anti-CHRM3 was incubated with 1:500 anti-Clara Cell Secretory Protein (CCSP; Merck Millipore cat#07– 623), 1:200 anti-alpha tubulin (Santa Cruz ...
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bioRxiv - Genomics 2021Quote: ... Prior to protein quantification milk was diluted 1:2 in assay buffer (Merck-Millipore, Burlington, USA). Concentrations for 15 cytokines (IL-1α ...
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bioRxiv - Immunology 2022Quote: ... Protein was digested by incubating membrane at 50°C for 1 h with proteinase K (Merck) diluted in PK-SDS buffer ...
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bioRxiv - Neuroscience 2020Quote: ... and mouse monoclonal IgG2A antibody to myelin Proteolipid Protein (clone PLPC1, MAB388, MERCK-Millipore, 1:100). All slides were mounted using Mowiol mounting medium (475904 ...
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bioRxiv - Microbiology 2023Quote: ... the pellets were washed first with 1× BugBuster® (10× Protein Extraction Reagent Novagen® Merck), then twice with water and once with 50 mM NaH2PO4 (pH 8) ...
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bioRxiv - Genetics 2023Quote: ... and ESGRO-2i Supplement Kit (1:1000, #ESG1121; Merck KGaA)] in a humidified incubator at 37 °C ...
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bioRxiv - Genetics 2023Quote: ... The screens were performed without feeder cells in M15 medium supplemented with 1000 U/ml leukemia inhibitory factor (Merck). Cells were plated on flasks coated with 0.1% gelatin solution (Merck) ...
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bioRxiv - Cell Biology 2023Quote: ... ESCs were maintained in culture using Dulbecco’s modified Eagle medium (DMEM) with leukemia inhibitory factor (LIF) (1,000 U/ml; Merck-Millipore), L-glutamine ...
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bioRxiv - Cell Biology 2020Quote: ... pre-cleared extracts (2 mg) were incubated for 4 hr at 4 °C with 2 μg of pan–ADP–ribose binding reagent (MABE1016, Merck) or normal rabbit IgG (2729S ...
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bioRxiv - Microbiology 2021Quote: ... The sonicated cellular extract was spun down and the supernatant has been incubated with His-binding resin (Merck 69670-5) for 10-30 min at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... Total protein concentration of lysates was determined using a bicinchoninic acid kit (Merck Life Science UK Ltd, Gillingham, UK) and samples diluted in Laemmli buffer ...
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bioRxiv - Immunology 2020Quote: ... All ELISAs were developed with 100 µl substrate solution per well containing 0.11 M sodium acetate (Merck), 0.1 mg/mL 3,5,3’,5’-tetramethylbenzidine (TMB ...
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bioRxiv - Neuroscience 2023Quote: ... Plasma ferritin was assessed in 324 samples via an ELISA (RAB0197-1KT, Merck Life Science UK Ltd). TBI was calculated using the below equation as proposed by Cook et al. ...
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bioRxiv - Immunology 2022Quote: Cell extracts (1 mg protein) were treated for 15 min at ambient temperature with benzonase (Merck-Millipore) and pre-cleared by incubation for 30 min at 4°C with 20 µl of protein A/G-Sepharose beads ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were detected with horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG antibody (1:8,000, Merck Millipore) and visualised with the LiteAblot Plus substrate (Euroclone) ...
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bioRxiv - Cell Biology 2022Quote: ... was seeded in T25 flasks coated with rat tail type I collagen (Merck Millipore), in EndoGro-MV medium (Merck Millipore ...
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bioRxiv - Cell Biology 2022Quote: ... Protein A and Protein G conjugated to HRP were purchased from Merck. DNA dye Hoechst 33342 was purchased from ThermoFisher Scientific and used at a final concentration of 5 μg/ml ...
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bioRxiv - Immunology 2020Quote: ... Thirty µl of Protein G (Protein G Sepharose Fast Flow, Merck, UK) slurry per sample was added in addition to 0.01% sodium azide and incubated on a roller overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Total protein concentration was quantified by means of BCA protein assay (Merck). 10-20 μg of protein was separated by size using a 4-12% Bis-Tris Plus gel ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Protein A and Protein G conjugated to HRP were purchased from Merck. DNA dye Hoechst 33342 was purchased from ThermoFisher Scientific and used at a final concentration of 5 µg/ml.
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bioRxiv - Biochemistry 2020Quote: ... the medium was replaced with a serum-free media supplemented with 2 nM brain-derived neurotrophic factor (BDNF) (Merck Millipore). After 2 days in the BDNF-containing media ...
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bioRxiv - Biophysics 2020Quote: ... the cells were incubated with a FCS-free media supplemented with 2 nM brain-derived neurotrophic factor (BDNF) (Merck Millipore). After 2 days ...
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bioRxiv - Neuroscience 2021Quote: ... The cell medium was aspirated from the wells and fresh medium with one of the factors (Bafilomycin, 200 nM, Merck Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... Once DRGs were removed and placed in ice-cold neuronal growth medium (Neurobasal medium, B27, and 10 ng/ml Nerve growth factor (NGF)-2.5 s (Merck Millipore), 2 mM L-glutamine ...
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bioRxiv - Cell Biology 2023Quote: HPAECs were seeded at the density of 10,000 cells/well in a pre-prepared 96-well plate containing 30μl of growth-factor reduced Matrigel (Merck, # CLS354230-1EA) per well (38) ...
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bioRxiv - Cell Biology 2022Quote: ... The total concentration of proteins in samples was determined using the Bicinchoninic Acid Assay Kit (Sigma Aldrich/Merck, Poznan, Polska). The samples containing 20 µg of protein were mixed with 4× Laemmli loading buffer (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... The clear cell-free extracts were immunoprecipitated using monoclonal antibodies against T18 tag following Protein G Immunoprecipitation Kit (Merck Inc) protocol ...
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bioRxiv - Immunology 2022Quote: ... an aliquot of the sample was taken for measuring the total protein concentration using a BCA assay kit (Merck Millipore). The remaining sample was heat-treated (70 °C ...
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bioRxiv - Plant Biology 2024Quote: ... Calibration of the column was performed using the Gel Filtration Markers Kit for Protein Molecular Weights 12,000-200,000 Da (#MWGF200; Sigma-Aldrich/Merck, www.sigmaaldrich.com).
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bioRxiv - Physiology 2022Quote: BACE-1 activity was assessed using a fluorescent assay kit (Merck), while plasma glycerol and plasma HDL-C were determined using colorimetric kits (Sigma) ...
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bioRxiv - Neuroscience 2020Quote: ... a short protein sequence expressed in the injected virus (1:400 rabbit anti P2A; ABS31, Merck, Darmstadt, Germany). In the third step ...
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bioRxiv - Immunology 2020Quote: ... Protein was concentrated to 1 ml using a Amicon Ultra-4 Ultracel 30 kDa spin concentrator (Merck Millipore) and the protein was separated on a HiPrep 16/60 Sephacryl S-200 HR column (GE Healthcare ...
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bioRxiv - Neuroscience 2022Quote: Rabies injected brains were immunohistochemically stained using antibodies against the 2A linker protein (Merck/ Millipore, ABS31, 1:2000) found in tTA expressing cells ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell lysates (25 µL) and bovine serum albumin (BSA) protein standards (0-1 mg/mL BSA (Merck, Germany) in resuspension buffer ...
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bioRxiv - Biochemistry 2020Quote: ... Next the medium was replaced with a serum-free media supplemented with 2 nM brain-derived neurotrophic factor (BDNF) (Merck Millipore). After 2 days in the BDNF-containing media ...
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bioRxiv - Molecular Biology 2021Quote: ... was centrifuged at 1500 rpm for 10 mins and the supernatant was used to measure inflammatory cytokines (IL-4, IL-5, IL-13, γ-INF) by LUMINEX multi-factor detection (MERCK). The cell pellets were fixed in 4% formaldehyde for Wright-Giemsa staining and total cells were counted and classified in each slide.
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bioRxiv - Molecular Biology 2021Quote: TEER measurements [22] were performed on 3rd day of incubation with growth factors and (or) different inhibitors by using Millicell ERS-2 Electrical Resistance System (Merck-Millipore). Each insert was measured in three different locations ...
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bioRxiv - Microbiology 2021Quote: ... Cytokine quantification was performed using a Human Cytokine/Chemokine/Growth Factor Panel A 48-Plex Premixed Magnetic Bead Multiplex Assay (Merck Millipore), using the Luminex MAGPIX System in 96-well plate format ...
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bioRxiv - Developmental Biology 2023Quote: ... 80% of complete medium was changed to either new complete medium without trophic factors or complete medium containing 5 ng/mL BDNF (GF029 Merck Millipore) or 100 ng/mL human PGRN (AG-40A-0188Y Adipogen) ...
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bioRxiv - Cell Biology 2019Quote: ... Collagen gels containing cells were prepared by mixing type I rat tail collagen solution (Merck) with 2 x concentrated DMEM (Merck) ...
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bioRxiv - Immunology 2024Quote: A multiplex biometric ELISA-based immunoassay was used according to the manufacturer’s instructions (MERCK cat. n. MTH17MAG-47K-05) using the Bio-Plex 200 instrument (Bio-Rad Laboratories) ...
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bioRxiv - Cancer Biology 2023Quote: ... 500 μg of protein were added to washed agarose protein A beads (Merck Millipore) and incubated on a rotating wheel for 1 h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... protein standards (Merck Sigma-Aldrich) specified in the Supplementary Material were separated on the same column.
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bioRxiv - Microbiology 2023Quote: ... protein A sepharose beads (Merck) were incubated with the cleared supernatant for an hour to remove non-specific binding ...
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bioRxiv - Pathology 2022Quote: ... The elution fraction containing His6-PhoP was dialyzed in Binding Buffer to remove the high concentration of imidazole and concentrated using an ultrafilter (Merck KGaA, Darmstadt, Germany). SDS-PAGE and Western blot with an anti-His tag antibody was used to confirm the purified protein (ABclonal ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cells were seeded in culture dishes and the specific pharmacological inhibitors radicicol (inhibitor of ATP-binding site of Hsp90, Sigma-Aldrich, Merck, Darmstadt, Germany), cyclosporine A (inhibitor of Cyp activity ...
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bioRxiv - Cancer Biology 2023Quote: ... 250 ng of pTOPflash (wt-TCF) or pFOPflash (mutated-TCF binding site, negative control) reporter plasmids (TOPflash luciferase reporter assay, Merck Millipore, Darmstadt, Germany) were transfected with Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Plant Biology 2024Quote: ... and then clarified via passing through Millex® -GP Fast Flow & Low Binding Millipore Express® PES Membrane 0.22 µm syringe filter unit (SLGP033RS, Merck Millipore, USA).