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Citations for Merck :
501 - 550 of 2648 citations for Rat Carboxypeptidase A3 Mast Cell CPA3 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Eluted proteins were concentrated using a 10K Amicon tube (Merck-Millipore, Darmstadt, Germany) and the buffer was changed to PBS ...
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bioRxiv - Pathology 2023Quote: ... proteins were electro-transferred to a polyvinylidene difluoride membrane (Merck Millipore, Darmstadt, Germany), which was incubated in the blocking buffer (5% non-fat milk ...
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bioRxiv - Plant Biology 2024Quote: ... Protein pellets were resuspended in 50 μl of 1.5% sodium deoxycholate (SDC; Merck) in 0.2 M EPPS-buffer (Merck) ...
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bioRxiv - Molecular Biology 2024Quote: ... FLAG-tagged proteins were detected using M2 anti-FLAG antibody (mouse, F1084, Merck) followed and fluorescent Anti-Mouse Alexa Fluor™ Plus 800 ...
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bioRxiv - Biochemistry 2024Quote: ... Immunoreactive proteins were visualized using Immobilon® Forte Western HRP Substrate (Merck Millipore) at the Imager Fluor-Chem M (Proteinsimple) ...
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bioRxiv - Microbiology 2024Quote: ... The pellet was resuspended in protein resuspension buffer (150 mM NaCl (Merck, USA), 5 mM EDTA (Merck ...
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bioRxiv - Molecular Biology 2024Quote: ... protein pellets were resuspended in 50 µl of 1.5% sodium deoxycholate (SDC; Merck) in 0.2 M EPPS-buffer (Merck) ...
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bioRxiv - Cancer Biology 2021Quote: ... and thereafter protein transferred onto 45 µm Polyvinylidene difluoride (PVDF) membrane (IPVH00010, Merck Millipore). Western blotting analysis was performed by using specific primary and secondary antibodies and signals visualized with chemiluminescence ...
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bioRxiv - Cell Biology 2020Quote: ... and nucleoproteins were extracted using a nuclear protein extraction kit (Merck Millipore, Darmstadt, Germany). Nucleoproteins were separated by electrophoresis on 6/12% sodium dodecyl sulfate (SDS ...
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bioRxiv - Cell Biology 2019Quote: ... Supernatant containing Matrigel proteins was discarded and pellets were lyzed in RIPA buffer (Merck), supplied with protease and phosphatase inhibitors (Merck) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... For IP with guinea-pig antibody protein A-conjugated magnetic beads (Merck Millipore, LSKMAGA10) were used ...
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bioRxiv - Developmental Biology 2020Quote: hESCs were harvested for protein extraction by addition of CelLytic M lysis buffer (Merck), freshly supplemented with protease inhibitors (PIC ...
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bioRxiv - Molecular Biology 2021Quote: ... proteins were eluted with 20 μl of 1 mg/ml 3xFLAG peptide (Merck, F4799) for 15 min at room temperature with vigorous shaking ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were concentrated with an Amicon Ultra-15 10 kDa centrifugal filter unit (Merck). Protein concentration was determined by UV-spectroscopy.
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bioRxiv - Immunology 2021Quote: ... Supernatants were first precleared with PureProteome™ Protein A/G agarose suspension (Merck Millipore) for 1 h ...
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bioRxiv - Biochemistry 2020Quote: ... The purified proteins were then concentrated on either Amicon (Merck Millipore, MWCO 10 kDa) or Vivaspin (Sartorius ...
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bioRxiv - Genetics 2021Quote: ... Proteins separated by SDS-PAGE were transferred onto a PVDF membrane (Immobilon-E, Merck) and blocked in 5% milk in Tris-Buffer Saline (TBS ...
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bioRxiv - Pathology 2021Quote: ... proteins were transferred from the gel onto to a polyvinylidene fluoride membrane (Merck Millipore) via electroblotting at 120 V for 60 minutes in transfer buffer containing 20% methanol ...
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bioRxiv - Systems Biology 2021Quote: ... Proteins were detected using a monoclonal anti-FLAG M2 antibody (F3165, Sigma/Merck Millipore) and an HRP-conjugated polyclonal goat anti-mouse antibody (P0447 ...
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bioRxiv - Bioengineering 2021Quote: ... the cDNA of the fusion protein was cloned in pET21a (Merck KGaA, Darmstadt, Germany). For preparative overexpression the E ...
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bioRxiv - Bioengineering 2021Quote: ... and supplemented with 10 µM/ml Rho-associated protein kinase inhibitor (Y-27632, Merck) for the first day.
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bioRxiv - Cell Biology 2021Quote: ... proteins were concentrated using Amicon Ultra-4 30 kDa centrifugal filter tubes (#UFC803024, Merck) and resuspended in sterile HBSS containing 1.2 mmol/l Ca2+ ...
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bioRxiv - Microbiology 2020Quote: ... Imidazole was removed from purified protein fractions using Amicon Ultra centrifugal filters (UFC505024, Merck) and washed three times and reconstituted in buffer D (50 mM Tris-HCl ...
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bioRxiv - Plant Biology 2020Quote: ... The purified protein was desalted using the Amicon Ultra-15 Centrifugal Filter Unit (Merck) and the desalted protein was used for activity assays ...
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bioRxiv - Microbiology 2020Quote: ... 40 µg proteins were mixed with acetone (Uvasol® for spectroscopy, Merck, Darmstadt, Germany) to obtain a final concentration of 80 % acetone (v/v ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were transferred onto a polyvinylidene difluoride (PVDF) membrane (Immobilon-PSQ PVDF membrane, Merck) by semi-dry turbo Western blot procedure (Trans-Blot Turbo Transfer System ...
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bioRxiv - Cancer Biology 2021Quote: ... An aliquot of extract containing 100 µg protein was then digested by benzonase (Merck) and precipitated by acetone ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was concentrated in centrifugal filter (Amicon Ultra-15, MWCO 30 kDa, Merck Millipore), aliquoted ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was concentrated in centrifugal filter (Amicon Ultra-15, MWCO 100 kDa, Merck Millipore), aliquoted ...
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bioRxiv - Biochemistry 2022Quote: ... Protein extracts were further processed in a Microcon-30 kDa Centrifugal Filter Units (Merck) in a tempered centrifuge at 24°C ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was concentrated in centrifugal filter (Amicon Ultra-15, MWCO 30 kDa, Merck Millipore), aliquoted ...
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bioRxiv - Biochemistry 2022Quote: ... 10 µM Mif2 protein per condition was immobilized on anti-FlagM2 agarose beads (Merck) for 1 h at 4 °C and 1200 rpm in a thermomixer ...
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bioRxiv - Systems Biology 2022Quote: ... proteins were reduced by addition of 5 mM DTT (Merck Sigma, Cat. No. 43815) or 5 mM TCEP (Merck Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... and immunoreactive proteins were visualised using Immobilon® Forte Western HRP Substrate (Merck Millipore). Densitometric analysis was carried out using the ImageJ 1.53c software ...
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bioRxiv - Plant Biology 2022Quote: ... proteins were further concentrated using Amicon Ultra-15 Centrifugal Filter Units (MERCK, Carrigtohill, Ireland) and stored at -20°C ...
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bioRxiv - Bioengineering 2022Quote: ... Proteins were blotted onto a 0.45 µm nitrocellulose membrane (Merck, Amersham Protran, Cat#GE10600002) for 2 hr at 4 °C and 30 V ...
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bioRxiv - Cell Biology 2022Quote: ... proteins were separated on SDS/PAGE gels and transferred onto a PVDF membrane (Merck). After blocking the membrane with 5% fat-free milk in TBST ...
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bioRxiv - Neuroscience 2021Quote: ... dilute proteins were concentrated using Amicon Ultra 15mL centrifugal filters (Merck Millipore, 10kDa NMWCO) and subjected to single-step buffer exchange into SEC running buffer (see SAXS method ...
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bioRxiv - Biochemistry 2021Quote: ... and the protein was concentrated using Amicon 3 kDa MWCO centrifugal filters (Merck Millipore). The protein concentration was calculated using the measured absorbance at 280 nm under native conditions and assuming an extinction coefficient of 19940 M-1 cm-1.
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bioRxiv - Immunology 2020Quote: ... Supernatant was first incubated with PureProteome™ Protein A/G agarose suspension (Merck Millipore) for 1 h to remove background signal ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were first precleared with PureProteome™ Protein A/G agarose suspension (Merck Millipore) for 1 h ...
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bioRxiv - Cancer Biology 2022Quote: ... The proteins were transferred from the gels to Immobilon PVDF membranes (Merck Millipore Ltd.). The membranes were immersed in appropriate blocking buffer (Tris-buffered saline 0.05% Tween 20 (TBST ...
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bioRxiv - Bioengineering 2019Quote: ... Eluted proteins were dialyzed using a 3 kDa membrane D-Tube Dialyzer (Merck, Germany) to remove the imidazole in Storage Buffer (200 mM ammonium acetate ...
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bioRxiv - Synthetic Biology 2020Quote: ... proteins were transferred to a polyvinylidene difluoride membrane (Immobilon-P; Merck Millipore, MA, USA) using a Trans-Blot® SD semi-dry transfer cell (Bio-Rad ...
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bioRxiv - Bioengineering 2020Quote: ... Protein expression was induced by the addition of 1 μg/mL doxycycline (D3447, Merck) in DMEM ...
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bioRxiv - Synthetic Biology 2021Quote: ... Proteins were concentrated by centrifugation with a 10 kDa cut-off membrane (Merck Millipore) to a volume of 300 µl ...
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bioRxiv - Neuroscience 2020Quote: ... A pre-clear step was performed by adding protein A-agarose beads (Merck Millipore) to the lysates ...
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bioRxiv - Biochemistry 2019Quote: ... The protein was concentrated by Amicon Ultra-15 Centrifugal Filters (10 KDa) (UFC901008, Merck) and diluted in buffer HT with 50 mM KCl for decreasing of KCl concentration to 90 mM ...
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Molecular Insights into the Recognition of Acetylated Histone Modifications by the BRPF2 BromodomainbioRxiv - Biochemistry 2022Quote: ... Purified proteins were concentrated using Amicon Ultra-10k centrifugal filter device (Merck Millipore Ltd.), and the concentration was determined using Bradford assay kit (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2022Quote: ... The eluted proteins were concentrated by Amicon Ultra Centrifugal Filter 50 kDa (Merck Millipore) and purified on a Superose 6 10/300 GL column (GE Healthcare ...