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Citations for Merck :
1 - 50 of 461 citations for RNA Amplification kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The resulting RNA was then subjected to amplification (Merck WTA2 Amplification Kit) to produce cDNA that was subsequently purified by column capture (Qiagen QiaQuick PCR purification kit ...
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bioRxiv - Microbiology 2023Quote: ... DNA and RNA was amplified using the Complete Whole Transcriptome Amplification kit (WTA2, Merck), and resulting PCR products were further purified and prepared for sequencing with the Nextera XT kit (Illumina) ...
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bioRxiv - Immunology 2024Quote: ... For FACS sorted cells RNA was amplified cDNA synthesized using a SeqPlex RNA Amplification kit (Merck Life Science, UK) as per manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... RNA was purified using the GenElute Mammalian total RNA Miniprep kit (MERCK). Samples from cell culture were harvested in the RNA extraction buffer supplemented with 10mM ß-ME ...
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bioRxiv - Evolutionary Biology 2021Quote: ... RNA was extracted using the GenElute™ Mammalian Total RNA Miniprep Kit (Merck) following manufacturer’s instructions ...
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Induction of autotetraploidy and microbiome associations mediate differential responses to pathogensbioRxiv - Plant Biology 2021Quote: ... RNA was extracted using the Spectrum Plant Total RNA Kit (Merck / MilliporeSigma, MO, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was extracted using a Spectrum Plant Total RNA kit (Sigma-Aldrich, Merck KGaA, Darmstadt, Germany).
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bioRxiv - Plant Biology 2023Quote: ... RNA was isolated using the SpectrumTM Plant Total RNA Kit (Sigma Aldrich, Merck KGaA, Darmstadt, Germany). The material was stored at −70 °C until use.
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bioRxiv - Synthetic Biology 2021Quote: ... PCR amplification was performed by KOD Hot Start DNA polymerase (Merck Millipore). All enzymes ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCR amplifications were performed using KOD Hot Start DNA polymerase (Merck Millipore). All restriction enzymes ...
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bioRxiv - Cell Biology 2022Quote: ... Virus amplification was performed in Sf9 TriEX cells (Novagen / Merck #71023-3) grown in TriEx media (Novagen / Merck #71022) ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA of cell cultured tumour cells was isolated using the GenElute Mammalian Total RNA Purification Kit (Merck) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... The Sigma Spectrum plant total RNA kit (Merck KGaA, Darmstadt, Germany) was used for RNA extraction that followed the manufacturer procedure ...
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bioRxiv - Genetics 2023Quote: ... using the Spectrum Plant Total RNA kit (Merck KGaA, Darmstadt, Germany). Gene expression was determined by RT-qPCR as previously described (Zenoni et al. ...
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bioRxiv - Cell Biology 2021Quote: The Magna RNA-binding protein immunoprecipitation kit (Merck Millipore, Billerica, MA, USA) was applied for the assay ...
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bioRxiv - Immunology 2022Quote: ... or with vaccine RNAs using RiboJuice™ mRNA Transfection Kit (Merck Millipore) according to the manufacturer’s instructions and incubated for 18 h ...
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bioRxiv - Developmental Biology 2021Quote: ... The amplification was done using 2 ng of DNA and KOD hot star (MercK). PCR fragments were sequenced by EurofinsGenomics ...
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bioRxiv - Molecular Biology 2020Quote: ... From the remaining nuclei RNA-IP was then undertaken with the MagnaRIP Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit protocol (17-700, Merck) from step I.3 ...
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bioRxiv - Plant Biology 2022Quote: A hole punch was used to obtain approximately 100 mg of leaf tissue from each sample (15 biological replicates per condition) and the total RNAs were extracted using the Spectrum Plant RNA kit (Merck, Spain). RNAs were quantified using the NanoDrop ND-1000 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... cDNA amplification was performed using KiCqStart® SYBR® Green qPCR ReadyMix™ (Merck, Germany). The mRNA primer designs (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2021Quote: RNA immunoprecipitation (RIP) assays were conducted using the EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Merck Chemicals (Shanghai Co., Ltd). The anti-YTHDC1 antibody for RIP was purchased from Cell Signaling Technology ...
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bioRxiv - Plant Biology 2023Quote: ... was used to synthesize DIG-labeled sense and antisense RNA probes using DIG RNA labeling kit (SP6/T7) (Merck KGaA, Darmstadt, Germany). The DIG-labeled RNA probes were purified using ProbeQuant G-50 Micro Colums (Cytiva ...
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bioRxiv - Plant Biology 2024Quote: ... according to manufactureres instruction using leaf RNA that had been extracted using the Spectrum Plant Total RNA Kit (Merck KGaA, Darmstadt, Germany). Constructs were amplified with the Phusion High-Fidelity DNA Polymerase (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2020Quote: ... The subsequential RNA immunoprecipitation (RIP) was performed using the Magna RIP kit (Merck Millipore) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: ... performed by the GeneElute Mammalian Total RNA purification kit (Sigma Aldrich-Merck KGaA Germany). The RNA was treated with deoxyribonuclease (DNAse ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Final concentration was measured by Qubit™ RNA BR Assay Kit (Thermo Fisher Scientific. RNA integrity was assessed by gel electrophoresis with 1:1 formamide (Merck Millipore) and 1 µl of loading dye on 1.5% agarose gel ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was extracted from snap-frozen and ground leaf tissue using the Spectrum™ Plant Total RNA Kit (Merck, Kenilworth, New Jersey, USA) with on-column DNase I digestion ...
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bioRxiv - Cancer Biology 2022Quote: RIP was performed according to the Magna-RIPTM RNA-binding protein immunoprecipitation kit (Millipore/Merck) as previously described (Papoutsoglou et al ...
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bioRxiv - Synthetic Biology 2023Quote: ... RNA was extracted from the microplate using the RNAspin Mini Kit (Merck; GE25-0500-71) and quantified using a NanoDrop™ Spectrophotometer (ThermoFisher) ...
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bioRxiv - Plant Biology 2023Quote: ... germinating seeds or rosette leaves using Spectrum™ Plant Total RNA Kit (Merck KgaA, Darmstadt, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... The amplification of ALBA genome sequences was carried out with high-fidelity KOD Hot Start DNA Polymerase (Merck Millipore), according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... in samples where biotin-streptavidin amplification was used before blocking with 10% chemiblocker (Merck Millipore, Billerica, MA, USA, #2170) for 2 hours at room temperature ...
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bioRxiv - Microbiology 2019Quote: Ribonucleoprotein complex immunoprecipitation (RIP) was performed using the EZ-Magna RIP RNA-binding protein Immunoprecipitation Kit (Merck Millipore Billerica ...
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bioRxiv - Cancer Biology 2022Quote: ... mRNA was extracted from cells using the GenElute mammalian Total RNA miniprep extraction kit (Merck, RTN70-1KT) and was reverse transcribed to synthesise cDNA using High Capacity cDNA Reverse Transcription Kit (ThermoFisher ...
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bioRxiv - Biochemistry 2022Quote: ... aeruginosa PAO1 served as a template for the amplification of the genes of interest via PCR using the KOD Xtreme polymerase (Novagen/Merck) and cloning primers containing the restriction sites (Eurofins Genomics) ...
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bioRxiv - Cancer Biology 2022Quote: An RIP assay was performed using a Magna RIP RNA binding protein immunoprecipitation kit (17-700; Merck Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... and fruits of three replicate were isolated by using RaFlex Total RNA isolation Kit (Merck Millipore, Massachusetts, USA) by following the standard protocol defined by the manufacturer ...
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bioRxiv - Molecular Biology 2019Quote: ... RIP-Seq experiments with UV cross-linking were performed using the Magna RIPTM RNA-Binding Protein Immunoprecipitation Kit (17-700 Merck). Briefly ...
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bioRxiv - Plant Biology 2022Quote: ... obtained by grinding in liquid nitrogen and using a SpectrumTM Plant Total RNA kit (Sigma-Aldrich, Merck KGaA, Darmstadt, DE) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: The protocol for detecting SINEUP RBPs was based on the protocol for the Magna ChIRP RNA Interactome kit (Merck Millipore) with the modification of cross-linking with 300 mJ/cm of 264 nm UV light (CL-1000 Ultraviolet Cross Link ...
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bioRxiv - Microbiology 2021Quote: Ago2 RIPs were performed on TREx-BCBL-1 RTA cells using EZ-Magna RIP RNA binding Immunoprecipitation Kit (Merck millipore) as per manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Total RNA was isolated from 100 mg of fresh root tissue sampled from three individual plants (biological replicates) using the Spectrum Plant RNA Purification Kit (Merck). RNA was treated with RNase-free DNase Set (Qiagen ...
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bioRxiv - Plant Biology 2022Quote: The m6A enrichment experiment was performed as described previously with minor modifications (Dominissini et al., 2013) and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Merck) was used following the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2022Quote: Hippocampal tissues from P40 WT mice were collected and lysed with IP lysis buffer in EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Merck-Millipore). YTHDF2 (Proteintech ...
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bioRxiv - Cancer Biology 2024Quote: ... total RNA was extracted (from TNBC cell lines or cryopreserved tumor tissue) using the GenElute mammalian total RNA miniprep kit (Sigma/Merck, RTN350-1KT) according to manufacturer’s instructions with an additional step of DNA digestion using the On-Column DNAse I digestion set according to manufacturer’s instructions (Sigma/Merck ...
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bioRxiv - Genomics 2022Quote: ... followed by quenching with final 1 x Glycine solution for 5 min at RT utilizing the Magna Nuclear RIP (Cross-Linked) Nuclear RNA-Binding Protein Immunoprecipitation Kit (Merck millipore, 17-10520). Cross-linked cells were then centrifuged at 800 x g at 4°C and washed three times with ice cold DPBS ...
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bioRxiv - Neuroscience 2019Quote: ... and transactivating RNAs (tracrRNA) (Merck) in combination with cas9 nuclease protein (Merck ...
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bioRxiv - Cell Biology 2023Quote: DNA-RNA Immunoprecipitation was performed using Anti-DNA-RNA Hybrid Antibody S9.6 (Merck Cat No MABE1095) as per Sanz and Chédin ...
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bioRxiv - Immunology 2023Quote: ... 1μl 20mM Tracr RNA (Sigma/Merck), 1μl Cas9 (diluted 1:3 in diluent B ...
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bioRxiv - Immunology 2019Quote: ... in combination with transactivating RNA (tracr) and synthetic guide RNAs specific to zebrafish egfra and erbb2 genes (Merck). Tyrosinase guide RNA was used as a control as described previously (Isles et al. ...