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Citations for Merck :
51 - 100 of 4575 citations for Protein Kinase A PKA Activity Assay Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... cells were fixed for 1 min in 4% formaldehyde and stained for AP activity using the Alkaline Phosphatase Detection Kit (Merck Millipore, Darmstadt, Germany).
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bioRxiv - Physiology 2023Quote: ... For citrate synthase activity, 15-30 µg protein was added to the reaction buffer (20 mM Tris-HCl, pH 8.0 (Merck), 0.42 mM acetyl-coenzyme A (Sigma) ...
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bioRxiv - Microbiology 2024Quote: ... The glycerol concentration in the medium was determined enzymatically using the recombinantly produced Saci_2033 glycerol kinase (see below) and ADP formation was quantitatively assayed spectrophotometrically as oxidation of NADH via pyruvate kinase (PK) (Merck, Germany) and lactate dehydrogenase (LDH ...
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bioRxiv - Cell Biology 2020Quote: Cellular Senescence Assay kit (Merck Millipore: KAA002) was used to detect senescent AML12 by SA β-Gal staining as per the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 μl MTT assay kit reagent (Merck) was then added to each well ...
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bioRxiv - Microbiology 2022Quote: ... and protein concentration was measured by Bradford assay (Merck, Gillingham, UK).
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bioRxiv - Biochemistry 2023Quote: In situ protein-protein interaction was evidenced using Duolink® Proximity Ligation Assay (Merck, DUO920082, DUO92004, DUO92008). Cells were fixed with formaldehyde and permeabilized using ice-cold 90% methanol ...
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bioRxiv - Cell Biology 2022Quote: Wound healing assays were performed in Corning® 6-well plates (Merck, UK). HDF and MSC were individually labelled with cell tracking dyes eBioscience™ CFSE (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: 200 μg Pol II was hyperphosphorylated by incubation with 280 units of human MAP Kinase 2/Erk2 Protein (Merck, cat. No, 14-550) in a total volume of 50 μl in kinase buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: RNA immunoprecipitation (RIP) assays were conducted using the EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Merck Chemicals (Shanghai Co., Ltd). The anti-YTHDC1 antibody for RIP was purchased from Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2022Quote: ... ATP:ADP ratio assays were performed using an ADP/ATP ratio assay kit (Merck, MAK135).
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bioRxiv - Genetics 2023Quote: ... Protein lysate was prepared in Radio-immunoprecipitation assay (RIPA) buffer (Merck Millipore) with a protease inhibitor cocktail (Roche ...
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bioRxiv - Microbiology 2023Quote: ... difficile using the succinate colorimetric assay kit (Merck) according to the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2023Quote: A multiplex assay kit (HNABTMAG-68K, Merck Millipore) was used to quantify Aβ40 ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein aggregates were removed by centrifugation (0.45 um filter plate, Merck, #MSHVN4550). From the soluble fraction the protein concentration was determined (BCA assay ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein aggregates were removed by centrifugation (0.45 um filter plate, Merck, #MSHVN4550). From the soluble fraction the protein concentration was determined (BCA assay ...
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bioRxiv - Molecular Biology 2020Quote: ... In each well of a 96 Well Corning Costar Assay Plate (Merck, Darmstadt, Germany) either 100 µL mAB 107 (for standardization ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein concentration of each lysate was determined using a BCA assay (Merck, 71285).
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bioRxiv - Cell Biology 2022Quote: ... Protein extracts were quantified using Direct Detect® Assay-free Cards (Merck; DDAC00010) or Pierce Bicinchoninic Bcid (BCA ...
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bioRxiv - Microbiology 2023Quote: ... and the protein concentration was determined by bicinchoninic acid (BCA) assay (Merck Millipore) and by UV-visible spectroscopy using an ε of 24 mM⁻¹·cm⁻¹ at 280 nm ...
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bioRxiv - Physiology 2023Quote: ... and protein concentration in the supernatant was determined by BCA assay (Merck B9643). Finally ...
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bioRxiv - Neuroscience 2021Quote: The activation of Caspase 1 in mouse brain samples were determined using Caspase 1 Colorimetric Assay Kit (YVAD, Merck Millipore, Burlington, MA) and following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... and a Lactate assay kit (MAK064, Merck, Darmstadt, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The live-dead cell viability assay kit (Merck Millipore), consisting of Calcein AM and Propidium iodide ...
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bioRxiv - Microbiology 2020Quote: ... A total of 50 μg of protein from each sample was transferred to a protein LoBind plate (Merck, NJ, USA). Protein was reduced with tris (2-carboxyethyl ...
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bioRxiv - Molecular Biology 2019Quote: Cells were plated in rectangular cell culture plates using Cell Comb™ Scratch Assay (Merck) and grown to 100% confluency ...
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bioRxiv - Cancer Biology 2021Quote: Intracellular protein was extracted from cells using the NucBuster Protein Extraction Kit (Merck) and quantified using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Final concentration was measured by Qubit™ RNA BR Assay Kit (Thermo Fisher Scientific. RNA integrity was assessed by gel electrophoresis with 1:1 formamide (Merck Millipore) and 1 µl of loading dye on 1.5% agarose gel ...
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bioRxiv - Physiology 2022Quote: ... Protein carbonylation was assessed with an Oxyblot protein oxidation detection kit (#S7150, Merck Millipore) following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... FK506 (inhibitor of FKBP activity, Sigma-Aldrich, Merck), VER (inhibitor of ATP-binding site of Hsp70 ...
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bioRxiv - Bioengineering 2021Quote: Bromocresol Purple (BCP) Albumin Assay Kit (Sigma-Aldrich, Merck, Germany) performed as per manufacturer’s instructions with plasma samples diluted 5-fold in ultrapure water ...
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bioRxiv - Molecular Biology 2023Quote: A Chromatin Immunoprecipitation (ChIP) Assay Kit (Merck Millipore 17-295) was used for all CTCF ChIP experiments following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... or 100 uM myosin light chain kinase inhibitor ML-7 (Merck 475880) and fixed for 20 min in 4% paraformaldehyde and 0.2% Tween 20 in PBS.
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bioRxiv - Neuroscience 2021Quote: ... mouse monoclonal anti-CaM Kinase II α subunit antibody (05-532, Merck), mouse monoclonal anti-GAD67 antibody (MAB5406 ...
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bioRxiv - Cell Biology 2023Quote: ... Aurora kinase inhibitor VX-680 (also known as MK-0457, Tozasertib) (Merck); [γ-32P] ATP (PerkinElmer ...
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bioRxiv - Immunology 2019Quote: Protein lysates from cells were obtained by lysis in radioimmunoprecipitation assay buffer (Merck Millipore, #R0278) with protease and phosphatase inhibitors (Roche ...
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bioRxiv - Cell Biology 2020Quote: ... the Akt/mTOR phosphoprotein magnetic bead panel 11-plex 96-well plate assay (Merck Millipore, Darmstadt, Germany). This assay uses paramagnetic beads (6.5 μm diameter ...
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bioRxiv - Cell Biology 2022Quote: Stress assays (wounding or uraemic serum addition) were performed in Corning® 6-well plates (Merck, UK). The HDF ...
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bioRxiv - Genetics 2020Quote: ... Chromatin immunoprecipitation was performed using Agarose ChIP Assay Kit (Merck Millipore). Briefly,107 cells were lysed by incubation on ice with 130 µl lysis buffer for 15 minutes ...
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bioRxiv - Cancer Biology 2020Quote: Chromatin immunoprecipitation assay was performed using a commercial kit (Merck-Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... and stained using the AldeRed ALDH detection assay kit (Merck SRC150) according to manufacturer specifications.
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bioRxiv - Physiology 2023Quote: ... glucose levels were determined using a Glucose (GO) assay kit (Merck). Blood insulin concentrations were measured by Rat Insulin ELISA kit (Mercodia) ...
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bioRxiv - Molecular Biology 2022Quote: Millipore Compartment Protein Extraction Kit (Merck-Millipore, Cat. #2145) was used to deplete cytosolic ...
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bioRxiv - Microbiology 2020Quote: ... To investigate the inhibitory activity of E-64 (Merck) and Z-VAD-fmk (Invivogen ...
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bioRxiv - Microbiology 2022Quote: ... and oxidase activity was determined using oxidase strips (Merck). Bacterial growth was evaluated on CM agar plates with different NaCl concentrations (0.5 - 5% (w/v ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cyclosporine A (inhibitor of Cyp activity, Sigma-Aldrich, Merck), FK506 (inhibitor of FKBP activity ...
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bioRxiv - Physiology 2022Quote: ... To detect total protein carbonylation by immunoblot we used the OxyBlot Protein Oxidation Detection kit (Merck) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... 500–1000 µg of total protein (estimated by Direct Detect® Infrared Spectrophotometer assay, Merck Millipore), were incubated overnight with 2 µg of antibody raised against an intracellular epitope in mouse NLGN1 (aminoacids 826 to 843 ...
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bioRxiv - Cancer Biology 2019Quote: The uncharged polystyrene 96-well plate were coated with different recombinant proteins-Collagen IV (Merck Millipore), P-Selectin ...
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bioRxiv - Cell Biology 2020Quote: ... ChIP assays were performed using a specific kit (Merck Millipore, Darmstadt, Germany). After crosslinking ...