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Citations for Merck :
151 - 200 of 1486 citations for Protein A G Magnetic Beads for IP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... and 20 µg protein extracts were separated with 10-15% SDS-PAGE gel and transferred to the PVDF membranes (Millipore ISEQ00010, Merck). Each membrane was blocked and slit into two parts ...
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bioRxiv - Biochemistry 2023Quote: ... 40 μg of protein was added to each well and run in Boric acid-Tris buffer (192 mM Boric acid, Merck; 1 mM EDTA ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitations were performed as previously described7 using Protein G Dynabeads (LifeTechnologies) coupled to Myc 4A6 antibody (Merck Millipore, 05-724) or FLAG M2 monoclonal antibody (Sigma ...
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bioRxiv - Immunology 2023Quote: ... MultiScreen® IP Filter Plates with hydrophobic polyvinylidene fluoride membrane (Merck Milipore, cat.: MAIPS4510) were pre-wet with 35% ethanol in sterile MiliQ water for 1 min and coated with 5 μg/mL of monoclonal mouse anti-pig IFN-γ antibody (clone P2F6 ...
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bioRxiv - Molecular Biology 2021Quote: ... and Milliplex Mouse Pituitary Magnetic Assay for GH (Merck) according to manufacturers’ instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... The glutathione-S-transferases (GST) fusion proteins were expressed in Escherichia coli and purified with glutathione-conjugated agarose beads (Merck) by standard procedures ...
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bioRxiv - Cell Biology 2023Quote: ... 250 µg of protein were incubated with 25 µl of pre-equilibrated anti-FLAG resin (M2 anti-FLAG beads, Merck) on a rotator at 4°C overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... Immunoprecipitation of cell lysates overexpressing FLAG-tagged or GST-tagged proteins were performed using anti-FLAG-M2 affinity beads (Merck) or glutathione-Sepharose beads (Cytiva).
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bioRxiv - Neuroscience 2023Quote: ... Protein G Dynabeads (catalogue # 10003D) were washed with 0.02% (v/v) PBS-Tween 20 (Tween-20: catalogue # P2285, Merck; 0.01M PBS) and incubated with 200µL of primary antibody mix (for antibody details see Supplementary Table 5 ...
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bioRxiv - Cell Biology 2024Quote: ... of cell lysates by incubating lysates with 0.5-2.5 µg of antibodies and 20 µl of protein G–Sepharose (Merck, GE17-0618-01) at 4°C for 2 h ...
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bioRxiv - Synthetic Biology 2022Quote: ... 20 g/L peptone (Difco) and 20 g/L glucose (Merck Millipore). Synthetic complete medium without uracil (SC-URA) ...
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bioRxiv - Cell Biology 2021Quote: ... Collagen G (Merck, Germany) gel is prepared according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... 4 g PFA (Merck) was dissolved in 80 ml PBS heated to 60 °C ...
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bioRxiv - Neuroscience 2022Quote: ... 25,000 cells were plated on ELISpot IP Filter Plates (PVDF membrane, 0.45 um, Merck; MSIPN4550) and fixed at 50°C for 1 h before treatment with 1 ug/ml proteinase K (Roche ...
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bioRxiv - Cell Biology 2020Quote: ... Stag-Tulp3 was then pulled down from the TEV digestion eluates by overnight 4°C rotational incubation using S-protein agarose beads (Merck, #69704). Beads were then eluted with Laemmli buffer and processed for SDS-PAGE in Novex Value 4-20% Tris-Glycine gels (Thermofisher) ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were pre-cleared at 4°C for 1 h on a rotating wheel using 30 µl Protein-S agarose beads (Merck Millipore) slurry ...
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bioRxiv - Physiology 2021Quote: ... Lysates were centrifuged (16,000 g, 10 min, 4°C) and supernatants measured for protein content using infrared spectroscopy (Merck Millipore, Darmstadt, Germany). Lysates were then reduced ...
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bioRxiv - Biochemistry 2023Quote: ... cells were harvested by centrifugation at 5,000 × g and 4°C for 5 min and lysed with BugBuster Protein Extraction Reagent (Merck Millipore, Burlington, MA) prepared in 20 mM sodium phosphate buffer (pH 7.4 ...
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bioRxiv - Microbiology 2023Quote: ... K2HPO4 2.9 g/L, VWR; di-ammonium citrate 0.7 g/L, Sigma-Aldrich; sodium acetate 0.26 g/L, Merck; glucose 1% (w/v), Merck ...
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bioRxiv - Microbiology 2023Quote: ... K2HPO4 2.9 g/l, VWR; di-ammonium citrate 0.7 g/l, Sigma-Aldrich; sodium acetate 0.26 g/l, Merck; glucose 1% (w/v), Merck ...
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bioRxiv - Immunology 2022Quote: Decidual cells were isolated as described above and incubated in the presence of fluorescence-labeled latex beads (50 beads per cell; latex beads, 1 µm, carboxylate-modified polystyrene, fluorescent yellow-green, Merck) for 2 hours at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... pH 2.0) for endogenous MDH2 IP and incubation with 250 μg/ml 3XFLAG peptide (Merck F3290) in the case of FLAG IP ...
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Regulatory T cells control type 1-driven immunopathology restraining GM-CSF-producing helper T cellsbioRxiv - Immunology 2024Quote: Treg cell-depletion was achieved by intraperitoneal (IP) injection 500 ng diphtheria toxin (DT) (Merck-Millipore) dissolved in of 150 µl of PBS on days 0 and 1 ...
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bioRxiv - Immunology 2020Quote: ... Paramagnetic bead (SP3 bead) and other general chemical reagents were from Merck, Dorset ...
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bioRxiv - Immunology 2022Quote: ... and purified by NiNTA beads (Ni-NTA beads, Merck, cat. 70666-4). IFNmod fused with SUMO was purified by the on-column cleavage using 50 mM Tris pH 8 ...
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bioRxiv - Microbiology 2020Quote: ... Immunoprecipitation of FLAG-tagged Hfq was performed by incubating each sample with 75 µl pre-washed (3 x resuspended in 1 ml lysis buffer and centrifuged at 10,000 rpm for 1 min) protein A sepharose beads (Merck/Sigma-Aldrich #P6649) for 45 min at 4 °C (rocking) ...
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bioRxiv - Bioengineering 2020Quote: Fibrinogen stock solutions were prepared by dissolving 1 g of human fibrinogen lyophilized from 20 mM citrate pH 7.4 solutions (Merck 341576, >90% clottable proteins) into distilled water as recommended by the manufacturer (final volume of app ...
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bioRxiv - Microbiology 2023Quote: ... Speed beads (400041, Merck) were used for instrument alignment and were run constantly during image acquisition for focusing and camera alignment ...
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bioRxiv - Bioengineering 2021Quote: ... 5.25 g of K2HPO4 (Merck) and 0.25 g of MgSO4 (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2021Quote: ... (M=100.04 g/mol, Merck) solvent and stirring for 24h ...
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bioRxiv - Physiology 2022Quote: ... Phosphomolybdic Acid Orange G (Merck AG ...
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bioRxiv - Microbiology 2022Quote: ... 8.77 g/L NaCl (Merck), 294 mg/L CaCl2(H2O)2 (Merck) ...
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bioRxiv - Cell Biology 2021Quote: ... TXA (Merck, 10 μg/g) or sodium chloride ...
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bioRxiv - Cell Biology 2020Quote: ... 0.01 g/ml CaCl2.2H2O (Merck) in PBS ...
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bioRxiv - Bioengineering 2020Quote: ... 5.25 g of K2HPO4 (Merck) and 0.25 g of MgSO4 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... 0.041 g/L KH2PO4 (Merck), 0.052 K2HPO4 (Merck) ...
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bioRxiv - Plant Biology 2021Quote: ... 0.1 g Myo-Inositol (Merck) and 0.8 or 1% Daishin agar (Duchefa ...
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bioRxiv - Microbiology 2022Quote: ... 5 g/l NaCl (Merck), 1 g/l K2HPO4-trihydrate (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... and Sera-Mag SpeedBead Carboxylate-Modified Magnetic Particles (Hydrophobic) (#GE24152105050350, Merck) for 10mins at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... and cultured in StemMACS iPS-Brew XF medium (Miltenyi Biotech) supplemented with 2 μM Thiazovivin (Merck Millipore), for 24 h after passaging with daily medium change ...
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bioRxiv - Molecular Biology 2020Quote: ... Chromatin-IP was carried using the ChIP assay kit (EZ-ChIP TM; Cat#17-295) from Merck Millipore ...
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bioRxiv - Immunology 2021Quote: ... in combination with multiscreen immunoprecipitation (IP) ELISpot polyvinylidene difluoride (PVDF) 96-well plates (Merck Millipore, Darmstadt, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... IP samples were resolved on 8%–12% SDS-PAGE and electroblotted onto Immobilon-P membranes (Merck Millipore) using a semidry transfer apparatus ...
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bioRxiv - Genetics 2022Quote: ... 50 μg of protein was used for gel electrophoresis in 10-14% SDS-PAGE gels and transferred to PVDF membranes (Merck Millipore, Billerica, MA, USA). After blocking in 5% defatted milk ...
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bioRxiv - Cell Biology 2020Quote: ... cells were washed in a magnetic separator (Merck Millipore, Burlington, MA, USA) with DMEM/F-12 ...
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bioRxiv - Biochemistry 2023Quote: ... A 1:1 mixture of magnetic hydrophobic and hydrophilic Seramag speedbeads (MERCK) were added at a 2:1 (w/w ...
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bioRxiv - Cell Biology 2023Quote: ... FLAG M2 beads (Merck M8823) were used ...
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bioRxiv - Biochemistry 2022Quote: ... 2.5 g/l D-glucose (Merck). U937 cells were differentiated to macrophages by 24 h incubation with 20 nM phorbol 12-myristate 13-acetate (PMA ...
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bioRxiv - Immunology 2020Quote: ... 0.20 g/L NaH2PO4 × H2O (Merck), 8.00 g/L NaCl (Sigma ...
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bioRxiv - Synthetic Biology 2021Quote: ... 4 g/l thiamine-HCl (Merck), and 40 g/l myo-inositol (Merck) ...