Labshake search
Citations for Merck :
401 - 410 of 410 citations for Protease since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... 10% glycerol) supplemented with 1 mg/ml lysozyme and one tablet of SIGMAFAST Protease Inhibitor Cocktail Tablets EDTA-Free (Merck KGaA, Germany), and disrupted by sonication (10 cycles of 30 sec at 40 % intensity in ice ...
-
bioRxiv - Cell Biology 2020Quote: ... All lysis buffers were supplemented with inhibitors of proteases (1/100 (v/v)) and phosphatases (1/50 (v/v)) (P8340 and P5726, Merck/Sigma-Aldrich, UK). Protein concentration was quantified using a BCA protein assay (23252 ...
-
bioRxiv - Cancer Biology 2023Quote: ... peptides were prepared by lysis of cells with a 5% SDS solution and Roche cOmplete™ Mini EDTA-free Protease Inhibitor Cocktail (Merck Darmstadt, Germany), followed by sonication (10 minutes ...
-
Variation of wine preference amongst consumers is influenced by the composition of salivary proteinsbioRxiv - Biochemistry 2023Quote: ... 120 μL supernatant was diluted with 120 μL 50 mM TEAB in 300 mM NaCl containing protease inhibitor cocktail (Merck & Co., New Jersey, USA). Proteins in 200 μL of the mixture were participated by 1 mL pre-chilled acetone at −20 °C overnight ...
-
bioRxiv - Biochemistry 2021Quote: ... 1 mM DTT) + 20 mM imidazole + protease inhibitors (10 μg/ml Pepstatin -P4265 Sigma-, 10 μg/ml Leupeptin −108975 Merck-, 1 mM AEBSF-A8456 Sigma-) and cells were lysed by lysozyme and sonication ...
-
bioRxiv - Developmental Biology 2021Quote: ... The calcium-free ringer solution was replaced with 1 mL of protease solution (72 μg/mL Liberase DH research grade from Merck/Sigma, 0.4U/mL DNaseI-Invitrogen). The embryos were incubated at 28.5C on an orbital shaker for 20 minutes ...
-
bioRxiv - Cell Biology 2019Quote: ... and resuspended in Lysis Buffer (Tris 10 mM pH 8, 2 mM EDTA) supplemented with Complete protease inhibitor (Roche, Merck, Sigma-Aldrich, l’Isle d’Abeau, France). After three cycles of freezing – thawing ...
-
bioRxiv - Molecular Biology 2023Quote: ... the eggs were transferred into centrifuge tubes containing 1 ml XBE2 + 10 µg/ ml protease inhibitor (aprotinin Sigma A6279, leupeptin Merck 108975, pepstatin Carl Roth 2936.1) + 100 µg/ ml cytochalasin D (Sigma C8273) ...
-
bioRxiv - Neuroscience 2020Quote: ... Cell lysis of all samples was performed after two washes with ice-cold PBS by incubating cells for 20 min in ice-cold 1% Triton X100 in PBS with protease inhibitor cocktails (Merck, Calbiochem set III, Cat. No. 539134), subsequent thorough scraping and sonication.
-
bioRxiv - Neuroscience 2020Quote: ... Bacteria were resuspended in (50mM HEPES, 250mM NaCl, 10% glycerol plus 1% Triton X-100, supplemented with protease inhibitors (Merck, Calbiochem set III, Cat. No. 539134) and subsequently sonicated ...