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Citations for Merck :
501 - 550 of 2050 citations for Primary Human Brain Microvascular Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were blocked using 2% normal goat serum and stained using the following primary antibodies in blocking solution overnight: anti-Ng2 (1:100, rabbit pAb, AB5320 Merck Millipore, Burlington, MA), anti-beta III tubulin (1:100 ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated o/n at 4°C with the primary antibodies (Rabbit-antiGFAP: MAB3402, Merck Millipore, 1:500; Mouse-antiNeuN: MAB377, Merck Millipore, 1:500) diluted in the blocking solution ...
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bioRxiv - Neuroscience 2024Quote: ... The tissue sections from the in vivo experiment were incubated with a rabbit primary antibody for GluR2/3 (Merck Millipore; AB1506; 1:200) overnight and then with a secondary goat anti-rabbit IgG (Cell Signaling Technology ...
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bioRxiv - Molecular Biology 2019Quote: Cells were plated in rectangular cell culture plates using Cell Comb™ Scratch Assay (Merck) and grown to 100% confluency ...
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bioRxiv - Developmental Biology 2021Quote: ... males were boosted with 100 units (U) of human chorionic gonadotropin (HCG: Chorulon®, Merck, NADA NO.140-927, Code No. 133754) and females were primed with 10U HCG ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... rat slices were exposed to 3 μM dihydroethidium (DHE) while in humans’ sections it was added 4 μM DHE (Merck, Darmstadt, Germany) for 30 minutes at 37ºC 51 ...
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bioRxiv - Neuroscience 2021Quote: ... The following primary antibodies were used: mouse monoclonal anti-SARM1 (Chen et al., 2011) (1:5000) and mouse anti-β-actin (Merck, A5316, 1:2000) as a loading control ...
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bioRxiv - Cancer Biology 2021Quote: ... blocked in 5% BSA and probed with primary antibodies overnight (anti-THEM6, ab121743, anti-CALR, ab22683, Abcam, Cambridge, UK; anti-mitochondria, MAB1273, Merck Millipore, Burlington, MA, USA). The next day ...
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bioRxiv - Molecular Biology 2022Quote: ... Organoid cells were counted using the Muse Cell Analyzer (Merck) and a total of 100,000 viable cells per well and in triplicates per treatment were seeded in conic v bottom 96-well plates (greiner bio-one) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were detached with non-enzymatic cell dissociation solution (Merck) or briefly (for Itgb4 staining only ...
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bioRxiv - Cell Biology 2020Quote: Eggs were collected from female Xenopus laevis frogs 16-18 hours after egg laying was induced by injection of 500 U of human chorionic gonadotropin (Chorulon from Merck, Sigma #CG10-10VL). Cytostatic factor-(CSF- ...
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bioRxiv - Bioengineering 2020Quote: Fibrinogen stock solutions were prepared by dissolving 1 g of human fibrinogen lyophilized from 20 mM citrate pH 7.4 solutions (Merck 341576, >90% clottable proteins) into distilled water as recommended by the manufacturer (final volume of app ...
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bioRxiv - Pathology 2021Quote: ... Cytokine and chemokine levels in monkey sera were measured using a MILLIPLEX MAP Non-Human Primate Cytokine Magnetic Bead Panel - Premixed 23 Plex - Immunology (Milliplex MAP kit, Merck Millipore, Burlington, MA), which includes the following 23 cytokines and chemokines ...
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bioRxiv - Molecular Biology 2022Quote: 200 μg Pol II was hyperphosphorylated by incubation with 280 units of human MAP Kinase 2/Erk2 Protein (Merck, cat. No, 14-550) in a total volume of 50 μl in kinase buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Developmental Biology 2023Quote: ... Leuplin for Injection Kit), a micro-infusion pump (ALZET Osmotic Pumps, iPRECIO SMP-200) with 15 IU/kg human FSH (hFSH, Merck Biopharma, Gonal-f) was embedded subcutaneously under anaesthesia and injected 7 µL/h for 10 days ...
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bioRxiv - Cell Biology 2019Quote: ... All mutations were confirmed by Sanger DNA sequencing and expression was confirmed by western blotting with anti-Myc primary antibody (1:2000 dilution; Merck Millipore #06-340, Billerica, MA).
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bioRxiv - Cell Biology 2022Quote: ... red blood cells were lysed using Red Cell Lysis buffer (Merck) and a cell count performed ...
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bioRxiv - Cancer Biology 2021Quote: ... Labeled cells and clones were filtered using a cell strainer (Merck) and seeded either as ‘one pot’ or ‘sequential layer’ 2D and 3D admixtures as detailed (Fig ...
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bioRxiv - Neuroscience 2019Quote: ... cells were seeded onto hanging PET cell culture inserts (Merck Millipore) at a density of 80,000 cells/insert ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells were passed through Flowmi cell strainers (40 μm, Merck) and counted using a Bürker chamber.
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bioRxiv - Cell Biology 2019Quote: ... and subsequently with 5IU of human chorionic gonadotropin (hCG) (Intervet, GesmbH) were mated and zygotes were isolated in M2 media (Merck Millipore, MR-015P-D) and cultured in KSOM medium (Cosmo Bio Co. ...
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bioRxiv - Molecular Biology 2024Quote: We used human APOL1 gene locus transgenic mice (BAC/APOL1 mice)17,45 with APOL1-G0 and APOL1-G1 heterozygotes (Merck & Co., Inc. (Rahway, NJ, USA)) ...
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bioRxiv - Neuroscience 2022Quote: ... The digested tissue was passed through a 40μm nylon cell strainer (Falcon) and red blood cells were lysed (Red Blood Cells Lysis buffer, Merck). After blocking with mouse FcR Blocking Reagent (MACS Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then trypsinized and placed in cell culture inserts (1 × 106 cells per insert) with PTFE membrane (Merck) in 6 well plates for overnight cultivation ...
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bioRxiv - Immunology 2022Quote: LDH activity was quantified in cell lysate of untreated cells and in all cell supernatants using the Cytotoxicity Detection Kit (LDH) from Merck, following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... Percentage of Ki67 negative and positive cells was determined from the fluorescence of cells in each sample analysed by Muse Cell Analyzer (Merck). Experiments were performed at least four times in duplicates for each treatment.
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bioRxiv - Cancer Biology 2019Quote: ... Percentage of phospho-AKT and phospho-ERK positive cells were determined from the fluorescence of cells in each sample analysed by Muse Cell Analyzer (Merck). Experiments were performed at least four times in duplicates for each treatment.
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bioRxiv - Developmental Biology 2021Quote: ... Cell survival was measured using Cell Proliferation Reagent WST-1 (Merck, USA) at a final dilution of 1:10 followed by incubation for half an hour and quantitation with a scanning multi-well spectrophotometer (SpectraMax-Molecular Devices ...
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bioRxiv - Cell Biology 2021Quote: ... and counting the number of cells using the Scepter Cell Counter (Merck), followed by seeding a known number of cells into 6-well plates containing growth medium ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cell viability was studied using Cell Counting Kit-8 (CCK8, Merck, Germany). VeroE6 cells were seeded at 10,000 cells per well of flat bottom 96-well plates (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were maintained at 28°C with Ex-Cell 420 media (Merck), with shaking at 120 rpm for suspension culture and/or grown as static adherent cells ...
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bioRxiv - Bioengineering 2021Quote: Culture of human BM-MSCs and NE-MSCs with biomaterials performed in Corning® Costar® ultra-low attachment 24 well plates (Merck KGaA, Darmstadt, Germany) with Gibco™ α-MEM (Thermo Fisher) ...
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bioRxiv - Biochemistry 2020Quote: hCMEC/D3 cells (Merck) were grown on pre-coated coverslips in a 12-well plate until 70% confluent ...
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bioRxiv - Microbiology 2021Quote: ... Vero E6 cells (Merck) were a gift from Christine Chable (CEMIPAI ...
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bioRxiv - Cancer Biology 2023Quote: COV362 cells (Merck, 07071910) were grown in DMEM (Corning ...
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bioRxiv - Neuroscience 2023Quote: SH-SY5Y cells (Merck), derived from the SK-N-SH neuroblastoma cell line ...
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bioRxiv - Systems Biology 2022Quote: ... Cell suspension samples were used for cell counting using Scepter™ 2.0 Automated Cell Counter with 60 μm sensors (Merck Millipore), for cell viability and cellular ATP assessments using CellTiter-Glo® Luminescent Cell Viability Assay (Promega ...
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bioRxiv - Microbiology 2022Quote: ... Three microliters of cell suspension (whole cell) or cell lysate were spotted on an Immobilon-PSQ transfer membrane (Merck Millipore Ltd.) and air dried ...
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bioRxiv - Neuroscience 2021Quote: HEK 293 cells were seeded in cell culture plates (Corning, Merck, Darmstadt, Germany) in the day before at the appropriate density ...
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A novel Hsp90 phospho-switch modulates virulence in the major human fungal pathogen Candida albicansbioRxiv - Microbiology 2020Quote: ... cells were diluted to 106 cells/ml in RPMI (Sigma-Aldrich/Merck # R7755) and incubated at 37°C for 24 hours in flat-bottom 96-well dishes for cell viability and biomass assays or on Thermanox™ coverslips for scanning electron microscopy ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Cell viability assays were carried out using WST-1 Cell Proliferation Reagent (Merck). A498 cells were plated onto clear 96-well plates at 4,000 cells per well and left to adhere for 24 hours ...
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bioRxiv - Developmental Biology 2022Quote: ... The cell suspension was subsequently filtered through a 40µm cell strainer (Merck CLS431750) and further diluted with 2ml FC media ...
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bioRxiv - Cell Biology 2023Quote: ... 1 x 105 cells of the hepatocyte cell line HepG2 (Merck, Darmstadt, Germany) were seeded in the wells of a 24-well plate 48 h before the experiment and incubated at 37°C in 2 ml HepG2 cell culture medium (Advanced DMEM ...
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bioRxiv - Cell Biology 2024Quote: ... cells were counted using a Scepter 2.0 Handheld Automated Cell Counter (Merck Millipore) and 60 μm Sensors (Merck Millipore) ...
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bioRxiv - Cell Biology 2022Quote: ... milk powder before incubation in primary and HRP-secondary antibodies (α-globin, sc-514378; β-globin, sc-21757; γ-globin, sc-21756 [Santa Cruz]; β-actin, A1978 [Merck]; BCL11A, ab19487 [Abcam]). Bands were visualized using enhanced chemiluminescence (G.E ...
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bioRxiv - Cancer Biology 2022Quote: ... stained with Muse Cell Cycle Reagent (The Muse® Cell Cycle Kit, Luminex, MCH100106) and analyzed with Muse Cell Analyzer (Merck, Darmstadt, Germany). Staining and cell cycle analysis procedure is detailed in Supplementary Information.
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bioRxiv - Immunology 2021Quote: ... red blood cells were lysed and cells were cultured in Iscove’s medium (Merck, Biochrom)+10% FBS (PAN Premium Lot ...
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bioRxiv - Microbiology 2020Quote: ... cells were dissociated by cell dissociation solution (Sigma-aldrich, Merck KGaA; cat. no. C5914). 2.5 μg/ml of human Fc tagged SARS-S1B and SARS2-S1B was preincubated with mAb at the indicated mAb:S1B molar ratios for 1 hour on ice and subjected to flow cytometry ...
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bioRxiv - Cell Biology 2022Quote: The cell cycle was determined using Muse Cell Cycle Kit (Luminex/Merck, Poznan, Poland) using the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: iPSC-derived RPE cells grown on 24-well PET hanging cell culture inserts (Merck) (pore size 0.4 μm ...