Labshake search
Citations for Merck :
101 - 150 of 1146 citations for Prestained Protein Standards since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... protein A sepharose beads (Merck) were incubated with the cleared supernatant for an hour to remove non-specific binding ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Standard ligation of restriction enzyme digest products was performed using the Roche Rapid Ligation Kit (Merck Life Sciences). All reagents were used in accordance with the manufacturer’s specifications.
-
bioRxiv - Microbiology 2019Quote: ... External linear matrix matched calibration was applied using single element manganese and iron standards (Merck-Millipore, Darmstadt, Germany), including quality control with LKSD1 and LKSD 4 certified lake sediment standards (Natural Resources Canada ...
-
bioRxiv - Neuroscience 2022Quote: ... as internal standard (IS) in 67% deionized water and 33% acetonitrile (ACN, 900667, CAS 75-05-8, Merck). The LTQ Orbitrap ELITE ETD (Thermo Scientific ...
-
bioRxiv - Cell Biology 2022Quote: ... the pieces were washed in buffer and submitted to standard procedures for embedding in paraffin (Paraplast® -Merck). Cross-sections of 5 µm thick pieces were subjected to hematoxylin-eosin staining ...
-
bioRxiv - Cell Biology 2024Quote: ... Standard immunostaining protocol was followed and the coverslips were mounted on glass slides using fluoroshield (#F6182, Merck, Germany). Images were captured using the 10X and 60X oil immersion objective of the Olympus FluoView 1000 confocal microscope (Olympus LifeScience ...
-
bioRxiv - Cell Biology 2020Quote: Proteins from 3T3-L1 fibroblast lysates extracted with Cytobuster protein extraction reagent (Novagen®, Merck). After cell disruption for 10 min at room temperature ...
-
bioRxiv - Cell Biology 2019Quote: ... protein lysates were cleared by centrifugation and protein concentration was measured using Coomassie reagent (Merck). Denatured ...
-
bioRxiv - Microbiology 2021Quote: ... These aliquots were kept on ice and were diluted with 50 μL of 0.1 M HCl (containing 250 μM Norvalin as internal standard, Merck). The samples were deproteinized by addition of 10 μL of cold 5-sulphosalicilic acid (SSA ...
-
bioRxiv - Molecular Biology 2022Quote: Chicken embryonal fibroblast cells (DF1; a kind gift from Dr Dylan Sweetman) were expanded in standard Dulbecco’s Modified Eagle Medium (DMEM; Merck Life Science UK Limited ...
-
bioRxiv - Systems Biology 2022Quote: Standard solutions of potassium (K+) and barium (Ba2+) were prepared from AnalaR salts (KCl and BaCl2) (Merck, Darmstadt, Germany). 18-crown-6 ether (99% pure ...
-
bioRxiv - Physiology 2022Quote: ... To detect total protein carbonylation by immunoblot we used the OxyBlot Protein Oxidation Detection kit (Merck) following the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2020Quote: ... Protein concentrations were estimated by the bicinchoninic acid (BCA) protein assay method (Sigma-Aldrich, Merck, USA). Proteins (50 μg ...
-
bioRxiv - Cell Biology 2020Quote: ... Lysates were measured for protein concentration using a Non-Interfering protein Assay kit (cat#488250, Merck Millipore ...
-
A novel Hsp90 phospho-switch modulates virulence in the major human fungal pathogen Candida albicansbioRxiv - Microbiology 2020Quote: ... 2.0 Protein Detection System (Merck Millipore), incubated with Clarity Western ECL (BioRad) ...
-
bioRxiv - Microbiology 2020Quote: ... carrying different versions of pUA1108 encoding folA or dfrA homologs was determined following the Clinical and Laboratory Standards Institute (CLSI) guidelines using microdilution tests in Mueller-Hinton broth (Merck) [42] ...
-
bioRxiv - Microbiology 2021Quote: Reaction products and standards were dissolved in 20% 1-propanol and separated on a 10 cm HPTLC Si-60 plates (Merck) using 1-propanol:acetone:water 9:6:4 (v:v:v ...
-
bioRxiv - Microbiology 2020Quote: ... 5 µl of standards or samples were injected onto SEQuant ZIC-pHILIC column (Merck, PEEK 150 × 2.1 mm, 5 µm). MS analysis was performed in negative-ion mode over the mass range from 200 to 1,000 m/z ...
-
bioRxiv - Cell Biology 2022Quote: ... and SDS-PAGE and immunoblots were performed by standard methods using a mouse monoclonal anti-MYC antibody (clone 4A6, 05-724, Merck).
-
bioRxiv - Microbiology 2021Quote: ... Amount of H2O2 produced was calculated by comparison to a standard curve that was generated using known concentrations of H2O2 (Merck).
-
bioRxiv - Biochemistry 2021Quote: ... were constructed using standard genetic engineering techniques with a His6 tag at the N-terminus using the pET28a vector (Novagen, Merck). The recombinant CAHS1 protein ...
-
bioRxiv - Cancer Biology 2020Quote: ... The cell pellets were digested with a standard volume of suprapur nitric acid and hydrogen peroxide (Merck Millipore, MA, USA) (2:1 v/v ...
-
bioRxiv - Microbiology 2022Quote: ... 1.5×104 conidia of each strain were inoculated in 300 µL of RPMI in the presence of 8 µg/mL voriconazole (European Pharmacopoeia, -EP- Reference Standard, Merck) and incubated for 48 hours at 37 °C with occasional shaking ...
-
bioRxiv - Synthetic Biology 2022Quote: ... Aliquots of our working standard were generated by spiking 50 μL of our generated VLPs into 950 μL of human plasma (Merck). The calibration curve samples and the aliquots of working standard were then tested using a cobas® 6800 system (Roche ...
-
bioRxiv - Synthetic Biology 2021Quote: ... GGA reactions were performed in a thermocycler according to a standard GGA protocol.52 The GGA products were transformed into NovaBlue cells (Merck), from which the plasmids were purified using the QIAprep Spin Miniprep Kit (Qiagen ...
-
bioRxiv - Developmental Biology 2021Quote: Genomic DNA was extracted from tumors using standard Phenol/Chlorophorm protocol and precipitated with Isopropanol in the presence of Pellet Paint (Merck). The amplification was done using 2 ng of DNA and KOD hot star (MercK) ...
-
bioRxiv - Microbiology 2019Quote: Standards of selected metabolites (Supplementary Table 1) were prepared at 10 µM in 80% acetonitrile (Hypergrade for LCMS LiChrosolv, Merck) and injected separately into a column connected to mass spectrometer interface ...
-
bioRxiv - Plant Biology 2022Quote: ... The difference in absorbance values were converted into PA equivalents using a standard curve of procyanidin B1 (Merck, Branchburg, NJ).
-
bioRxiv - Plant Biology 2023Quote: ... Cell debris was removed with centrifugation and 2+2 μl of extracts and a commercial pinosylvin standard (ArboNova) were applied on TLC plates Silica gel 60 F254 (Merck), which were developed with chloroform ...
-
bioRxiv - Immunology 2023Quote: ... The iron content was determined by comparing the samples against a standard curve of serially diluted ammonium ferric citrate (F5879, Merck).
-
bioRxiv - Plant Biology 2023Quote: ... the Immunoprecipitation (IP) was performed following a standard IP protocol and using the following antibodies: H3 (Reference: 07-690, Merck), H3K9me2 (Reference ...
-
bioRxiv - Neuroscience 2023Quote: The viability of SH-SY5Y cells following incubation with fibrils formed in the absence/presence of gangliosides was determined using a standard MTT cell proliferation assay (Merck).
-
bioRxiv - Microbiology 2024Quote: ... Detection was performed via refractive index (RI) and UV (210 nm) and compared with standards of ethanol (Uvasol for spectroscopy, Merck), acetaldehyde (Reagent plus ...
-
bioRxiv - Plant Biology 2023Quote: ... the Immunoprecipitation (IP) was performed following a standard IP protocol and using the following antibodies: H3 (Reference: 07-690, Merck), H3K9me2 (Reference ...
-
bioRxiv - Biochemistry 2023Quote: In situ protein-protein interaction was evidenced using Duolink® Proximity Ligation Assay (Merck, DUO920082, DUO92004, DUO92008). Cells were fixed with formaldehyde and permeabilized using ice-cold 90% methanol ...
-
bioRxiv - Genomics 2020Quote: ... The protein solutions were subsequently desalted and protein amount was estimated using Direct Detect cards (Merck Millipore, Darmstadt). All samples were freeze dried before further processing ...
-
bioRxiv - Cell Biology 2023Quote: Protein carbonyl formation by oxidative reactions was assessed with the OxyBlot Protein Oxidation Detection Kit (S7150, Merck Millipore), according to manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2021Quote: ... A standard curve drawn with serial dilution of pNPP and a known quantity of ALP from bovine intestinal mucosa (Merck KGaA). Then ...
-
bioRxiv - Cell Biology 2020Quote: Flp-In™T-REx™293 and HEK293 cell lines were grown under standard conditions (37°C, 5% CO2) in Dulbecco’s modified Eagle’s medium (Merck-Sigma-Aldrich, D5796). For Flp-In™T-REx™293 the medium was supplemented with 10% fetal bovine serum (FBS) ...
-
bioRxiv - Immunology 2021Quote: ... was used for m6A immunoprecipitation (m6A-IP) using an m6A antibody (Synaptic, 202003) following the standard protocol of the Magna MeRIP m6A Kit (Merck Millipore). RNA was enriched through RNA Clean & Concentration-5 (Zymo Research ...
-
bioRxiv - Neuroscience 2020Quote: Samples boiled in 6x Laemmli buffer were subjected to standard SDS-PAGE (10%) prior to being electrophoretically blotted onto Immobilon-P polyvinylidene difluoride membranes (Merck, UK). Ponceau S staining (Merck ...
-
bioRxiv - Neuroscience 2020Quote: Samples boiled in 6× Laemmli buffer were subjected to standard SDS-PAGE (10%) and electrophoretically blotted onto Immobilon-P polyvinylidene difluoride membranes (Merck, UK). Total protein was quantified using Ponceau S staining (Merck ...
-
bioRxiv - Physiology 2020Quote: ... Zn concentrations were measured by atomic absorption spectrometry (AAS) (NovAA 350; Analytik Jena AG) applying a certified AAS Zn standard material (Merck 109953, Merck Millipore) after microwave wet digestion (Ethos 1 ...
-
bioRxiv - Microbiology 2022Quote: ... cultures were maintained at 30 °C in MGY M9 medium (10 g/L glycerol, 1.25 g/L yeast extract, M9 salts) or Standard I Nutrient Agar (Merck, Darmstadt, Germany) supplemented with 1% glycerol ...
-
bioRxiv - Biochemistry 2021Quote: FLAG-CAHS1 was constructed using standard genetic engineering techniques with an N-terminal FLAG using a pT7-FLAG-1 vector (Sigma-Aldrich, Merck). The bacterial cells of E ...
-
bioRxiv - Immunology 2019Quote: ... and viral titers were determined in Vero cells using a standard plaque assay at day 5 post-infection with crystal violet staining (Merck Millipore). The viral titers were determined in aliquots of harvested medium ...
-
bioRxiv - Pathology 2021Quote: ... The tissues were cut into 6-µm slices and then stained for lipids according to the standard ORO staining protocol (ORO, Merck, Germany). The samples were rinsed with 60% isopropanol and stained with filtered ORO solution (0.5% in isopropanol followed by 60% dilution in distilled water ...
-
bioRxiv - Microbiology 2020Quote: ... rhizoxinica were grown at 30 °C in MGY M9 medium (10 g/L glycerol, 1.25 g/L yeast extract, M9 salts) or Standard I Nutrient Agar (Merck, Darmstadt, Germany) supplemented with 1% glycerol.
-
bioRxiv - Physiology 2021Quote: ... Resulting lipid films were dissolved in 1 mL of n-hexane containing a C19:0 as internal standard (1.03 μg mL−1, CAS number 1731-94-8, Merck, Darmstadt, Germany) with addition of 200 μL of a solution of potassium hydroxide (KOH ...
-
bioRxiv - Cancer Biology 2023Quote: ... Conjugated AB were eluted in 30 μL antibody stabilizer solution (Standard Biotools, CA, USA) supplemented with 0.05 %sodium azide (Merck, Darmstadt, Germany) to a total volume of 50 μL stored in low attachment 1 mL Eppendorf tubes.