Labshake search
Citations for Merck :
151 - 200 of 1262 citations for Prestained Protein Peptide Standards since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2024Quote: ... Peptides were then desalted using styrene divinylbenzene-reverse phase sulfonate Stage-tips (Merck, Darmstadt, Germany) according to the manufacturer’s protocol ...
-
bioRxiv - Developmental Biology 2021Quote: ... A standard Western blot protocol was used with anti-HA(12CA5) (1:1000, Merck #11583816001), anti-Actin(20-33 ...
-
bioRxiv - Physiology 2022Quote: ... 34.5 mg TSP (Trimethylsilyl propanoic acid; used as NMR standard) and 100 mg NaN3 (Merck) were added 59 ...
-
bioRxiv - Biochemistry 2022Quote: ... After the standard procedure of Ni-NTA purification (Ni-NTA beads, Merck, cat. 70666-4) and Sumo protease cleavage (in-house production ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Commercial standards of OCFAs (Odd Carbon Straight Chains Kit containing 9 FAs, OC9, Merck, Germany) were converted into their FAMEs using the same method employed with the yeast samples ...
-
bioRxiv - Plant Biology 2021Quote: ... Pure standards of (Z)-3-hexenyl acetate (Z3HAC, 98 %, CAS number 3681-71-8, Merck) was used in different concentrations ...
-
bioRxiv - Microbiology 2022Quote: ... Selenite solutions were prepared from a 1,000 mg/L standard solution (Tritrisol-Merck, Darmstadt, Germany). Appropriate dilutions were made to prepare the standards ...
-
bioRxiv - Biochemistry 2023Quote: ... After the standard Ni-NTA purification process using Ni-NTA beads (Merck, cat. 70666-4) and subsequent Sumo protease digestion (concentration of sumo protease at 1:200) ...
-
bioRxiv - Biophysics 2024Quote: ... phantoms were prepared following standard protocols 74 using agar mixed with intralipid (both Merck, UK) to mimic tissue-like scattering with red-coloured synthetic fibres (Smilco ...
-
bioRxiv - Physiology 2024Quote: ... The recovery rate for reference materials (Mercury ICP Standard 1000 mg L−1 Hg, Merck) was above 95%.
-
bioRxiv - Neuroscience 2021Quote: ... The Aβ stock solution was prepared by solubilizing the Aβ25-35 peptide (Sigma-Aldrich; Merck KGaA) in sterile deionized water to a concentration of 1 mM and then incubated at 37°C for 3 days to allow self-aggregation before treatment ...
-
bioRxiv - Neuroscience 2020Quote: ... The Aβ stock solution was prepared by solubilizing the Aβ25-35 peptide (Sigma-Aldrich; Merck KGaA) in sterile deionized water to a concentration of 1 mM and then incubated at 37°C for 3 days to allow self-aggregation before treatment ...
-
bioRxiv - Plant Biology 2020Quote: ... The digested peptides were further filtered with Amicon ultra centrifugal filters (10kD MWKO, Merck Millipore, MA), dried under vacuum extensively to remove residual primary amine from reagents ...
-
bioRxiv - Systems Biology 2022Quote: ... Peptide fractions were eluted using MeOH buffer containing trifluoroacetic acid (TFA) (Merck Sigma, Cat. No. 302031), FA or ABC ...
-
bioRxiv - Immunology 2022Quote: ... The proteolytically cleaved peptides were extracted and desalted using ZipTip μC18 tips (Merck Millipore, Darmstadt, Germany). The peptide lysates were re-suspended in 15 µl 0.1% formic acid and analysed by nanoliquid chromatography mass spectrometry (UltiMate 3000 RSLCnano ...
-
bioRxiv - Biophysics 2022Quote: ... Both recombinant and synthetic lyophilized peptides were dissolved in 6M guanidine thiocyanate (Merck KGaA, Darmstadt, Germany) and incubated overnight at 1250 rpm ...
-
bioRxiv - Cell Biology 2023Quote: ... pH 2.0) for endogenous MDH2 IP and incubation with 250 μg/ml 3XFLAG peptide (Merck F3290) in the case of FLAG IP ...
-
bioRxiv - Microbiology 2021Quote: ... the ICP-MS was calibrated with a series of multi-element standard solutions (Merck ME VI), and a series of Ca2+ standard solutions (SCP Science PlasmaCAL) ...
-
bioRxiv - Developmental Biology 2022Quote: ... Samples were stained with hematoxylin and eosin according to standard protocol and mounted with Depex (Merck). Samples were imaged using Pannoramic P1000 slide scanner (3DHistech).
-
bioRxiv - Biochemistry 2023Quote: ... All analysed metals were quantified using ICP multi-element standard solution IV (Merck KGaA, Darmstadt, Germany) as a standard.
-
bioRxiv - Cell Biology 2022Quote: ... Protein A and Protein G conjugated to HRP were purchased from Merck. DNA dye Hoechst 33342 was purchased from ThermoFisher Scientific and used at a final concentration of 5 μg/ml ...
-
bioRxiv - Immunology 2020Quote: ... Thirty µl of Protein G (Protein G Sepharose Fast Flow, Merck, UK) slurry per sample was added in addition to 0.01% sodium azide and incubated on a roller overnight at 4°C ...
-
bioRxiv - Immunology 2022Quote: ... Total protein concentration was quantified by means of BCA protein assay (Merck). 10-20 μg of protein was separated by size using a 4-12% Bis-Tris Plus gel ThermoFisher Scientific ...
-
bioRxiv - Cell Biology 2023Quote: ... Protein A and Protein G conjugated to HRP were purchased from Merck. DNA dye Hoechst 33342 was purchased from ThermoFisher Scientific and used at a final concentration of 5 µg/ml.
-
miR-125-chinmo pathway regulates dietary restriction dependent enhancement of lifespan in DrosophilabioRxiv - Molecular Biology 2020Quote: ... Labelled peptides were separated on Chromolith Caprod RP-18e HR capillary column (150 × 0.1 mm; Merck Millipore) using linear gradient of buffer B (98% ACN and 0.05% TFA in water ...
-
bioRxiv - Microbiology 2022Quote: ... 100 µg of the dried peptides were dissolved in 90 µl of 0.2 M EPPS buffer (MERCK)/10% acetonitrile ...
-
bioRxiv - Pathology 2019Quote: ... The extracted peptides were purified using C18 Zip-TIP pipette tips according to the manufacturer’s instructions (Merck Chemicals ...
-
bioRxiv - Biochemistry 2019Quote: Crude peptides were purified by reverse-phase high-performance liquid chromatography using a Chromolith Prep column (Merck) on a Prominence LC-20AP system (Shimadzu ...
-
bioRxiv - Biochemistry 2019Quote: Peptides were spotted at 40 µg per origin and dried on thin-layer cellulose plates from Merck. Electrophoresis was run in 1% ammonium carbonate pH 8.9 at constant 1 kV for 25 min 40,41 ...
-
bioRxiv - Immunology 2020Quote: ... MHC–peptide complexes were eluted by repeated addition of 0.2% trifluoroacetic acid (TFA, Merck, Whitehouse Station, NJ). Peptides were purified by ultrafiltration using centrifugal filter units (Amicon ...
-
bioRxiv - Biochemistry 2023Quote: ... the peptides were desalted and intact glycopeptides were enriched by ZIC-HILIC beads (Merck, Product no. 1.50458.0001) according to our previous study[25].
-
bioRxiv - Bioengineering 2022Quote: ... Protein concentrations were determined with the BCA protein assay kit (Novagen, Merck KGaA), and protein homogeneity was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ...
-
bioRxiv - Cancer Biology 2021Quote: Intracellular protein was extracted from cells using the NucBuster Protein Extraction Kit (Merck) and quantified using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) ...
-
bioRxiv - Neuroscience 2022Quote: ... Protein concentration was estimated using BCA protein assay kit (Merck, 71285-3, Germany).
-
bioRxiv - Neuroscience 2021Quote: ... protein concentration was quantified using a Sigma-Aldrich BCA protein assay kit (Merck). Subsequently ...
-
bioRxiv - Microbiology 2020Quote: Multi-element calibration standard for calibrating inductively coupled plasma mass spectrometry (ICP-MS) was purchased from Merck. LB medium and Tryptic Soy Broth were purchased from BD and Merck ...
-
bioRxiv - Plant Biology 2021Quote: ... Quantification of vicianin was carried out using a dilution series of an amygdalin standard (Sigma-Aldrich/Merck), as this cyanogenic di-glucoside is structurally comparable to vicianin and expected to behave similarly with respect to their degree of ionization and UV absorption.
-
bioRxiv - Cancer Biology 2020Quote: Total RNA was isolated following a standard phenol-chloroform extraction protocol using TRI- reagent (Merck, Darmstadt, Germany) with subsequent LiCl (Carl Roth ...
-
bioRxiv - Cell Biology 2023Quote: ... The cell proliferation assay was done using the standard MTT assay (Sigma-Aldrich/Merck, Catalog No. 11465007001). MTT assay was performed on each well following the manufacturer’s protocol.
-
bioRxiv - Molecular Biology 2023Quote: ... medium was changed and replaced with standard growth medium complemented with 200 µM biotin (Merck, B4639-1G) for 1 hour ...
-
bioRxiv - Cell Biology 2022Quote: ... Standard minimal medium (SMM) consisted of 0.67% yeast nitrogen base without amino acids (Merck, product number Y0626), supplemented with all standard amino acids [bought from Sigma (now Merck)] at 76 mg/l except leucine ...
-
bioRxiv - Physiology 2020Quote: ... Total proteins from the supernatant were quantified using a Bradford Protein Assay Kit (Merck) and subjected to electrophoresis on 15% SDS–PAGE gels ...
-
bioRxiv - Physiology 2022Quote: ... Protein carbonylation was assessed with an Oxyblot protein oxidation detection kit (#S7150, Merck Millipore) following the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: ... 500 μg of protein were added to washed agarose protein A beads (Merck Millipore) and incubated on a rotating wheel for 1 h at 4°C ...
-
bioRxiv - Microbiology 2021Quote: Peptides EcFtsN1-32-C and VmFtsN1-29-C were labelled with maleimide-Atto 495 (Merck Life Science UK). EcFtsA1-405 and VmFtsA1-396 were buffer exchanged into binding buffer (50 mM HEPES/KOH ...
-
bioRxiv - Microbiology 2021Quote: Peptides EcFtsN1-32-C and VmFtsN1-29-C were labelled with maleimide-Atto 495 (Merck Life Science UK). EcFtsA1-405 and VmFtsA1-396 were buffer exchanged into binding buffer (50 mM HEPES/KOH ...
-
bioRxiv - Microbiology 2020Quote: ... Peptides were dissolved in 2% acetonitrile containing 0.1% trifluoroacetic acid and desalted using C18 ZipTips (Merck Millipore, Germany). Each sample was independently analysed on a Q-Exactive hybrid quadrupole-orbitrap mass spectrometer (Thermo Scientific ...
-
bioRxiv - Biochemistry 2021Quote: ... The peptides were diluted to 50 µg/mL in 10 mM sodium acetate pH 5 (Merck, Darmstadt, Germany) and injected overflow cell two of each channel to a final signal of 3400 RU for L-CDP1 and 1300 RU for L-CDP7 ...
-
bioRxiv - Microbiology 2024Quote: ... and subsequently eluted in 150 μL of wash buffer with 500 ng/μL FLAG peptide (Merck, Darmstadt, Germany) by rotation on a rotator for 2 h at 4 °C ...
-
bioRxiv - Microbiology 2023Quote: ... protein A sepharose beads (Merck) were incubated with the cleared supernatant for an hour to remove non-specific binding ...