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Citations for Merck :
51 - 100 of 299 citations for Porphobilinogen deaminase HMBS Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Human heat-aggregated IgG immune complexes (ICs) were prepared by heating human IgG1 isotype control (Merck) for 20 min at 63°C.
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bioRxiv - Bioengineering 2019Quote: ... human fibronectin (Merck-Millipore, Watford, UK) was premixed with Laponite gels to a final concentration of 50 µg/mL ...
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bioRxiv - Microbiology 2019Quote: ... Binding of human FH (Merck Millipore) was detected with Alexa-Fluor-488 labeled human FH ...
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bioRxiv - Immunology 2022Quote: ... Purified C1q from human serum (Merck) was used as a control.
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bioRxiv - Systems Biology 2020Quote: ... anti-human MLKL (Merck Millipore, MABC604), phospho anti-human RPB1 S2 (Millipore ...
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α-catenin links integrin adhesions to F-actin to regulate ECM mechanosensing and rigidity-dependencebioRxiv - Cell Biology 2021Quote: ... Human plasma full-length fibronectin (Merck) was added to the dish at a final concentration of 10 μg/μl for a 1h incubation at 37°C ...
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bioRxiv - Immunology 2023Quote: Human IFNβ (IF014, Merck, KGaA, Darmstadt), IFNα2a (11100-1 ...
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bioRxiv - Immunology 2024Quote: ... Human MC lines HMC-1.1 (Merck) and HMC-1.2 (Merck ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 5 μg/ml human insulin (Merck), and 50 μM hydrocortisone (Cayman ...
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bioRxiv - Biochemistry 2021Quote: ... The His6-tag fusion proteins were purified with a cOmplete™ His-tag purification resin (Roche, Merck). The fusion proteins were cleaved by incubation with thrombin protease (Sigma-Aldrich ...
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Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: ... plasmid coding for 6x-His-Cas9 were transformed in Rosetta DE3 Novagen competent cells (Merck Millipore, USA). Protein production was induced using autoinduction medium (Formedium ...
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bioRxiv - Biochemistry 2023Quote: His-tagged proteins were labeled using Ni-NTA-ATTO 550 or Ni-NTA-ATTO 647 from Merck Group ...
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bioRxiv - Genetics 2023Quote: ... imidazole was removed from the eluted His-SIRT5 using ultra-0.5 centrifugal filter units (UFC501096, Merck Millipore). 500 µl of the recombinant SIRT5 elution fractionation was added to the ultra-0.5 centrifugal filter units and centrifuged at 14,000 x g for 15 min at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... The lysate was then incubated with 2 mL pre-washed HIS-Select Cobalt Affinity Gel (Merck, UK) with rotation for an hour ...
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bioRxiv - Cell Biology 2023Quote: ... to validate the gene expression the plasmid (vide-supra) was transfected into HEK293 cells using PEI (Sigma-Aldrich/Merck Cat. No. 49553-93-7) following an optimized version of the standard protocol(Rajendra ...
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Incidence of an intracellular multiplication niche amongst Acinetobacter baumannii clinical isolatesbioRxiv - Microbiology 2021Quote: A549 (human epithelial lung cell line) and EA.hy926 (human endothelial somatic cell line) cells were bought from Merck company and ATCC ...
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bioRxiv - Molecular Biology 2022Quote: CobB driven deacetylation of His-PrsAc was analyzed by Western blot with anti-acetyl lysine antibodies (Sigma/Merck) and mass spectrometry ...
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bioRxiv - Zoology 2020Quote: ... recombinant RhATG5 and RhATG5191-199Δ were affinity-purified using Ni-NTA His•Bind Resin (Merck-Millipore, Darmstadt, Germany). Recombinant RhATG5 and RhATG5191-199Δ were then incubated with 10µg μ-calpain (Merck ...
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Oxidative and non-oxidative active turnover of genomic methylcytosine in distinct pluripotent statesbioRxiv - Cell Biology 2020Quote: ... 10 μg/ml human holo-Transferrin (Merck), 12.5 mg/ml AlbuMAX I (Life Technologies ...
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bioRxiv - Cell Biology 2019Quote: ... and human plasma full-length fibronectin (Merck) was added to the dish at a final concentration of 10 µg/µl for a 1h incubation at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... human recombinant insulin (100 µg/mL; Merck), recombinant human insulin-like growth factor 1 (hIGF1 ...
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bioRxiv - Molecular Biology 2020Quote: ... Recombinant human TNFα (GF023) was from Merck. Purified anti-His tag mouse antibody was from BioLegend (USA) ...
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bioRxiv - Cell Biology 2021Quote: ... and human plasma full-length fibronectin (Merck) was added to the dish at a final concentration of 10 μg/μl for a 1h incubation at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... mouse anti-human α5 IgG1 (JBS5; Merck), mouse anti-human α5 IgG3 (P1D6 ...
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bioRxiv - Physiology 2022Quote: ... labelled human mitochondria primary antibody (Merck, MAB1273), 1:500 for 48 h at 4°C and washed with PBS overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... rat anti-human β1 IgG1 (AIIB2; Merck), mouse anti-human β1 IgG1 (Lia1/2 ...
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bioRxiv - Microbiology 2023Quote: ... mouse anti-human α5 IgG3 (P1D6; Merck), mouse IgG1 (JC1 ...
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Chromatin priming elements direct tissue-specific gene activity prior to hematopoietic specificationbioRxiv - Genomics 2023Quote: ... 180⍰µg/ml Human transferrin (Merck, T8158), 20% D4T Conditioned Media ...
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bioRxiv - Immunology 2023Quote: ... polyclonal anti-human PI3K p85 (Merck Millipore), anti-PI3 Kinase p110α (C73F8 ...
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bioRxiv - Cell Biology 2024Quote: ... or 5µg/cm2 human-plasma fibronectin (Merck) overnight in 4°C ...
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bioRxiv - Bioengineering 2020Quote: ... and the membrane was incubated with primary antibody (anti-his tag, 1:1000, Merck Millipore, Merck KGaA, Darmstadt, Germany) diluted in 2 % skim milk for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... rh-HI was prepared in either 20% acetic acid (v/v from acetic acid glacial, Merck KGaA, Darmstadt, Germany), 0.3 M NaCl (Merck KGaA ...
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bioRxiv - Cancer Biology 2023Quote: ... Cdk1 and Cdc25B cDNAs were subcloned in frame with the N-terminal His-tag into pRSETa (Novagen, Merck Biosciences). Cdc25B cDNA was also subcloned into pcDNA3 without (pCdc25B) ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were clarified by centrifugation (10,000 x g, 30 min, RT) and incubated for 1 hour with Ni-NTA His bind resin (Merck) rolling at RT ...
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bioRxiv - Microbiology 2023Quote: ... the cells were incubated with F(ab’)2-Goat anti-Human IgG-FITC (Merck Goat anti-Human IgG, F(ab’)2 ...
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bioRxiv - Physiology 2021Quote: Human thrombin was purchased from Merck (Cramlington, UK) and reconstituted in double distilled water (ddH2O) ...
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bioRxiv - Microbiology 2021Quote: ... or polyclonal anti-human antibody (I2136, Merck, Germany) was coupled simultaneously in four flow cells ...
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bioRxiv - Cell Biology 2022Quote: ... and anti-human nuclei (Merck, MAB4383, 1:250) were diluted in the blocking solution and incubated overnight on a shaker at 4◦C ...
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Kinetochore- and chromosome-driven transition of microtubules into bundles promotes spindle assemblybioRxiv - Cell Biology 2022Quote: ... human Ndc80 siRNA (HA12977117-004; Merck, Darmstadt, Germany), human ON-TARGET PRC1 siRNA (L-019491-00-0020 ...
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bioRxiv - Developmental Biology 2021Quote: ... 0.1% aprotinin and 0.1% Human Insulin Solution (Merck). Medium was changed every other day with EHT medium.
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bioRxiv - Cancer Biology 2019Quote: ... mouse anti-human nuclear antigen (1:100; Merck), Alexa Fluor 647-conjugated rat anti-CD45 (1:100 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HTF medium (EmbryoMax Human Tubal Fluid; Merck Millipore) was mixed 1:1 with mineral oil (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... Normal human epidermal keratinocytes were obtained from Merck. All lines were maintained in 3:1 DMEM (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... growth in LB and human serum (Merck #H4522) was measured ...
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bioRxiv - Neuroscience 2023Quote: ... R&D, AF2408), Goat-Anti Human SOX17 (1:100, R&D, AF1924) or Mouse Anti-Human Beta-Tubulin (1:1000, Merck, T8660) primary Chicken Anti-Human MAP2 (1:2000 ...
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bioRxiv - Molecular Biology 2023Quote: ... stained with anti-human IFN-γ and anti-human IL2 antibodies for 40 min at RT in 0.2% saponin (Merck, Germany) in PBS (Gibco ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 ug inactive MEK1 (C-terminally His-tagged) in a total volume of 40 μL buffer (20 mM Hepes pH 8 (MERCK, Sigma Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... and applied directly onto a column of 2 ml pre-equilibrated Ni-NTA beads (cOmplete His-Tag Purification Resin from Merck). After several washes with high salt (500 mM NaCl) ...
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bioRxiv - Molecular Biology 2022Quote: ... the supernatant was loaded on to a gravity flow-based Ni-NTA metal affinity column (2 ml beads, cOmplete His-Tag Purification Resin from Merck), equilibrated and washed with 10 column volumes of buffer A containing 5 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... The His6-tagged proteins were purified using Immobilised Metal Affinity Chromatography (IMAC) with Ni-NTA His•Bind® Resin (Merck). Fractions containing the protein of interest were then dialysed against low-salt (50mM Tris pH8.5 ...